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Search Results: 1 - 10 of 215985 matches for " Carlos E Solarte Portilla "
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Characterization and genetic evaluation of Holstein cattle in Nari o, Colombia Caracterización y evaluación genética del ganado Holstein en Nari o, Colombia Avalia o e caracteriza o do gado Holandês, no departamento de Nari o, Col mbia
Carlos E Solarte Portilla,Gema L Zambrano Burbano
Revista Colombiana de Ciencias Pecuarias , 2012,
Abstract: Objective: to characterize and genetically evaluate the Holstein population located in the high tropics of Nari o province, Colombia, in order to propose an improvement program that meets the region's needs and conditions. Methods: first, values of heritability (h2), repeatability (R), and genetic correlations (Г) between milk production (PL), milk fat percentage (PG), milk protein percentaje (PP), open days (DIAB), number of services per conception (SPC), calving interval (IEP), and several shape or phenotypic variables were estimated. Historical information was collected from 296 farms between 1999 and 2006. Subsequently, the milk production control was conducted until 2010. An Animal Model was used to estimate the genetic parameters and genetic value. Results: a group of 22 males and 350 females was identified for the highest genetic values for productive and reproductive traits as well as anatomical conformation. Conclusion: intensive dissemination of this animal group to initiate the Holstein breeding improvement process in the dairy areas of Nari o was recommended. Objetivo: caracterizar y evaluar genéticamente la población Holstein en el Trópico Alto de Nari o, para proponer un programa de mejoramiento acorde con las necesidades y condiciones de esta región. Métodos: primero se estimaron los valores de heredabilidad (h2), repetibilidad (R), y correlaciones genéticas (Г) entre las variables producción de leche (PL), porcentaje de grasa en leche (PG), porcentaje de proteína en leche (PP), días abiertos (DIAB), numero de servicios por concepción (SPC), intervalo entre partos (IEP), y algunas variables de conformación o tipo. La información histórica se recolectó en 296 fincas entre los a os 1999 y 2006. Posteriormente se efectuó el control de producción hasta el a o 2010. Para la estimación de los parámetros y del valor genético se utilizó un Modelo Animal. Resultados: se identificó un grupo de 22 machos y 350 hembras con los valores genéticos más altos para los rasgos productivos, reproductivos, y de conformación anatómica. Conclusión: se recomendó la difusión intensiva de este grupo de animales con el fin de iniciar el proceso de mejoramiento genético del ganado Holstein en la cuenca lechera de Nari o. Objetivo: caracterizar e avaliar geneticamente o gado Holandês no Trópico Alto de Nari o (Col mbia), com o intuito de propor um programa de melhoramento que esteja de acordo com as necessidades e condi es da regi o. Métodos: estimaram-se os valores de herdabilidade (h2), repetibilidade (R) e correla es genéticas (Г) entre as variáveis: produ o de
Kappa casein genotypes and curd yield in Holstein cows
Zambrano Burbano,Gema L; Eraso Cabrera,Yohana M; Solarte Portilla,Carlos E; Rosero Galindo,Carol Y;
Revista Colombiana de Ciencias Pecuarias , 2010,
Abstract: the objective of this study was to relate kappa casein (csn3) genotypes with curd yield (rc) and total milk protein (ptp) in holstein cows located in the high tropics in narino, colombia. twenty seven animals were used to establish the mentioned relationships. the genotype of each animal was determined by pcr - sscp. variables were analyzed using a linear model which included the fix effects of genotype, lactation stage, and their interaction. age of the cow and fat percentage in milk were used as covariates. the results for rc indicate no interaction between genotype and lactation stage. age was not statistically significant (p>0.05), while fat percent and genotype were significant (p<0.05). the tukey - kramer test indicated differences between the bb genotype, compared to homozygous aa and heterozygous ab. the bb genotype resulted in the best performance, requiring the least amount of milk to produce one kg of curd. as for protein content, differences were significant (p<0.05) for the effect of genotype and lactation stage: the homozygous bb had the highest percentage of milk protein during the final (third) stage of lactation.
Kappa casein genotypes and curd yield in Holstein cows Relación entre los genotipos de la kappa caseína de vacas holstein y el rendimiento en cuajada Rela es entre os genótipos da kappa caseína em vacas holstein a rendimento de requeij o
Gema L Zambrano Burbano,Yohana M Eraso Cabrera,Carlos E Solarte Portilla,Carol Y Rosero Galindo
Revista Colombiana de Ciencias Pecuarias , 2010,
Abstract: The objective of this study was to relate kappa casein (CSN3) genotypes with curd yield (RC) and total milk protein (PTP) in Holstein cows located in the high tropics in Narino, Colombia. Twenty seven animals were used to establish the mentioned relationships. The genotype of each animal was determined by PCR - SSCP. Variables were analyzed using a linear model which included the fix effects of genotype, lactation stage, and their interaction. Age of the cow and fat percentage in milk were used as covariates. The results for RC indicate no interaction between genotype and lactation stage. Age was not statistically significant (p>0.05), while fat percent and genotype were significant (p<0.05). The Tukey - Kramer test indicated differences between the BB genotype, compared to homozygous AA and heterozygous AB. The BB genotype resulted in the best performance, requiring the least amount of milk to produce one kg of curd. As for protein content, differences were significant (p<0.05) for the effect of genotype and lactation stage: the homozygous BB had the highest percentage of milk protein during the final (third) stage of lactation. El presente estudio tuvo como objetivo determinar las relaciones entre los genotipos para Kappa Caseína (CSN3), el rendimiento industrial en cuajada (RC) y el porcentaje total de proteína (PTP) en vacas Holstein del Trópico Alto de Nari o-Colombia. El genotipo de cada animal fue determinado molecularmente con la técnica PCR - SSCP. Para establecer las relaciones antes indicadas se utilizaron 27 unidades experimentales. Las variables fueron analizadas mediante un modelo lineal en el que se incluyeron los efectos fijos del genotipo, el tercio de lactancia, la interacción entre estos dos factores y como covariables, la edad del animal y el porcentaje de grasa en la leche. Los resultados para RC indicaron que no existe interacción entre los genotipos y el tercio de lactancia. La edad del animal no fue estadísticamente significativa (p>0.05), mientras que la covariable porcentaje de grasa y el genotipo resultaron significativos (p<0.05). La prueba estadística de Tukey - Kramer indicó diferencias entre el genotipo BB, respecto al homocigoto AA y al heterocigoto AB, siendo el primero el de mejor rendimiento, al requerir la menor cantidad de leche para producir un kilogramo de cuajada. En cuanto al porcentaje de proteína, se encontraron diferencias estadísticamente significativas (p<0.05) únicamente por efecto del genotipo y del tercio de lactancia, siendo el homocigoto BB el que presentó mayor porcentaje de proteína en el tercer tercio
Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, caviidae) in Colombia
Burgos-Paz, William;Cerón-Mu?oz, Mario;Solarte-Portilla, Carlos;
Genetics and Molecular Biology , 2011, DOI: 10.1590/S1415-47572011005000057
Abstract: the aim was to establish the genetic diversity and population structure of three guinea pig lines, from seven production zones located in nari?o, southwest colombia. a total of 384 individuals were genotyped with six microsatellite markers. the measurement of intrapopulation diversity revealed allelic richness ranging from 3.0 to 6.56, and observed heterozygosity (ho) from 0.33 to 0.60, with a deficit in heterozygous individuals. although statistically significant (p < 0.05), genetic differentiation between population pairs was found to be low. genetic distance, as well as clustering of guinea-pig lines and populations, coincided with the historical and geographical distribution of the populations. likewise, high genetic identity between improved and native lines was established. an analysis of group probabilistic assignment revealed that each line should not be considered as a genetically homogeneous group. the findings corroborate the absorption of native genetic material into the improved line introduced into colombia from peru. it is necessary to establish conservation programs for native-line individuals in nari?o, and control genealogical and production records in order to reduce the inbreeding values in the populations.
Efecto del tama?o de camada y número de parto en el crecimiento de cuyes (Cavia porcellus Rodentia: caviidae)
Burgos-Paz,William; Solarte-Portilla,Carlos; Cerón-Mu?oz,Mario;
Revista Lasallista de Investigación , 2010,
Abstract: guinea pig is a species with a great potential to produce animal origin protein, given its precocity and fast growth, but just a few reports evaluate the growth of this species and its reproductive characteristics. one way to describe the growth of guinea pigs is by the use of mathematic non-lineal models, previously used to study many other species, given their easy calculations and data adjustment. objective. to describe the growth curve of female guinea pigs by the use of a non linear model and evaluate the correlation of the breed's size and the delivery number on the parameters of the curves, weight and maturity at different ages. materials and methods. 2505 weight records of 501 female guinea pigs were analyzed in the delivery and the weaning, at four, eight and twelve weeks of age. the curve was taken by the use of a von bertalanffy's non linear model. the significance of the independent variables was determined, maturity at 90 (m90) and 120 days (m120), and the weight at 15 (p15), 30 (p30), 60 (p60) and 90 days (p90) by the use of a mixed model that included the effects of the breeds' size, delivery number, contemporary group and common environment. results. the variance analysis determined that there is a correlation between the breed's size and the delivery number for the β1, m90, m120, p15, p30 p60 y p90 variables; while there was no correlation for the weight at 120 (p120) and 180 days (p180), and the animals had a similar growth. conclusions. von bertalanffy's non linear model was useful to adjust the weights of the female guinea pigs and the modeling of the growth of that species. on the other hand, it was evidenced that a higher number of deliveries brought a faster maturity of young guinea pigs.
Identificación de polimorfismos del gen de la Kappa caseína bovina: Nari o-Colombia
Carlos Eugenio Solarte Portilla,Carol Yovanna Rosero Galindo,Heiber Cárdenas Henao,William Orlando Burgos Paz
Revista Lasallista de Investigación , 2009,
Abstract: Introducción. El uso de tarjetas para la colección, almacenamiento y conservación de sangre no es frecuente en investigaciones de biología molecular, donde se prefiere la recolección y conservación en tubos Vacuntainer con acido etilen-diamino-tetraacético como anticoagulante. Sin embargo, por las condiciones topográficas de la zona de estudio, el uso de metodologías convencionales, con trans-porte refrigerado de las muestras, resulta poco apropiado por las dificultades de acceso a las fincas y las grandes distancias entre hato y hato. Objetivo. Estandarizar la técnica (reacción en cadena de la polimerasapolimorfismos de cadena simple) para la identificación de polimorfismos del gen de la kappa- caseína en ejemplares de las razas Holstein, Normando, Jersey y Pardo Suizo en la cuenca lechera del Trópico Alto de Nari o. Material y métodos. Se tomaron muestras de sangre de 1087 ejemplares, utilizando tarjetas para la recolección, almacenamiento y preservación del tejido y su posterior análisis en el laboratorio. Resultados. Para obtener productos amplificados de alta calidad fue suficiente un disco de 1.2 mm y un tiempo de separación electroforética de 16 horas a 160V. Conclusión. Esta investigación permite concluir que las tarjetas fueron un medio eficiente, ya que facilitan el transporte y almacenamiento de tejido sanguíneo y evitan el uso de refrigeración para la conservación de la muestra durante períodos largos de tiempo.
Efecto del tama o de camada y número de parto en el crecimiento de cuyes (Cavia porcellus Rodentia: caviidae)
William Burgos-Paz,Carlos Solarte-Portilla,Mario Cerón-Mu?oz
Revista Lasallista de Investigación , 2010,
Abstract: Introduccion. El cuy es una especie con un gran potencial para la produccion de proteina de origenanimal, gracias a su alta precocidad y rapido crecimiento pero son pocos los reportes que evaluan el crecimiento de esta especie y su relacion con caracteristicas productivas. Una forma para describir el crecimiento es mediante modelos matematicos no lineales, los cuales se han empleado en multiples especies, dada su facilidad de calculo y ajuste de los datos. Objetivo. Describir la curva de crecimiento de cuyes hembra mediante un modelo no lineal, y evaluar la relacion que tiene el tamano de camada (TC) y numero de parto (NP) en los parametros de las curvas, peso y madurez a diferentes edades. Materiales y metodos. Se analizaron 2505 registros de pesaje de 501 cuyes hembra, en el nacimiento, el destete, a cuatro, ocho y doce semanas de vida del animal. La curva se llevo a cabo mediante el modelo no lineal de Von Bertalanffy. Se determino la significancia de las variables dependientes, madurez a los 90 (M90) y 120 dias (M120), ademas del peso a los 15 (P15), 30 (P30) 60 (P60) y 90 dias (P90), mediante un modelo mixto que incluyo los efectos de tamano de camada, numero de parto, grupo contemporaneo y ambiente comun. Resultados. El analisis de varianza permitio determinar que existe una relacion entre TC y NP, para las variables à1, M90, M120, P15, P30 P60 y P90, mientras que para el peso a los 120 (P120) y 180 dias (P180), no se encontro relacion y los individuos mostraron un crecimiento similar. Conclusiones. El modelo de Von Bertalanffy permitió ajustar los pesos de cuyes hembra y la modelación del crecimiento en esta especie. Por otro lado, se observó que al aumentar el número de partos, las crías alcanzan más rápido su madurez.
Caracterización molecular de tres líneas de Cavia porcellus mediante la aplicación de AFLP
Solarte-Portilla,Carlos; Cárdenas-Henao,Heiber; Rosero-Galindo,Carol; Burgos-Paz,William;
Revista Colombiana de Ciencias Pecuarias , 2007,
Abstract: molecular markers are a powerful tool to determine genetic variability within and among populations, but for the cavia porcellus there are no reports on the use of these techniques. three populations, two native and another one, genetically improved which was obtained by crossing native and peruvian animals and submitted to genetic selection through several generations were analyzed by means of aflp markers. five primer’s combinations recommended for rodenthia were used, but only one allowed to establish significant differences (p<0.01) according to unbiased nei′s distance value. both native populations were grouped in a cluster genetically distant from the genetically improved animals. this showed that foreign animals absorbed the native populations. the average heterosigosity between 0.48% and 14.48% and the percentage of polymorphisms between 0.00% and 39.65% allow to conclude that there was a low variability between the populations, but the population genetically improved was the most polymorphic. the low variability within the improved animals it can be explained because of the intensive selection procedures use with them, whereas within the native populations can be explained because of their very low populations effective size. these results suggest that there is a need to restate the genetic improvement and preservation programs of the native cavia porcellus in the southwest region of colombia.
Polimorfismos en la longitud de fragmentos amplificados (AFLP′s) a partir de muestras de sangre almacenadas en tarjetas FTA? para la especie Cavia porcellus Lin. (Rodentia: Caviidae)
Burgos-Paz,William; Rosero-Galindo,Carol; Cárdenas-Henao,Heiber; Solarte-Portilla,Carlos;
Revista Colombiana de Ciencias Pecuarias , 2007,
Abstract: a methodology that includes the use of fta? (whatman bioscience, cambridge) to collect and store animals` blood samples and the procedures to extract and to get aflp markers is presented in this paper. a review of the literature indicates that there are no reports concerning both aspects for the cavia porcellus case. to reach our goal blood samples of three populations - two native ones and other genetically improved- were obtained through heart puncture. this blood was stored in the fta cards in order to extract, purify, amplify and analyze their dna forms. all of the animals came from "botana" farm of the universidad de nari?o, located in pasto, colombia. for amplifying the aflp one, three and five 1.2 mm fta disks of approximately 75 ng of dna per disk where used. the tests indicated that the best products to amplify and to visualize the aflp where those ones obtained from samples of three fta disks per animal. this suggests that 75 ng of dna per animal is enough to generate aflp of high quality in the cavia porcellus` genome. we recommend the use of fta cards to carry out genetic analyses in the cavia porcellus, including the methodology modifications presented in this paper.
Polimorfismos en la longitud de fragmentos amplificados (AFLP′s) a partir de muestras de sangre almacenadas en tarjetas FTA para la especie Cavia porcellus Lin. (Rodentia: Caviidae) Amplified Fragments Length Polymorphisms (AFLP′s) from blood samples stored in FTA cards for Cavia porcellus
William Burgos-Paz,Carol Rosero-Galindo,Heiber Cárdenas-Henao,Carlos Solarte-Portilla
Revista Colombiana de Ciencias Pecuarias , 2007,
Abstract: Una manera eficaz de establecer el grado de variabilidad entre y dentro de poblaciones, es a través del análisis de polimorfismos de ADN con marcadores moleculares como los AFLP`s. En este artículo se presenta una metodología que combina la utilización de tarjetas de FTA (Whatman Bioscience, Cambridge) para colección y conservación de muestras de sangre, con los procedimientos de extracción de ADN y obtención de marcadores AFLP′s, aspectos sobre los cuales no existen antecedentes para la especie Cavia porcellus. Se utilizaron muestras de ADN procedentes de tres poblaciones, dos criollas y una mejorada genéticamente obtenida a partir de un pie de cría procedente del Perú y sometida a selección en Colombia durante varias generaciones. Todos los animales procedieron de la Granja "Botana", propiedad de la Universidad de Nari o, Pasto-Colombia. Para la detección de polimorfismos en la longitud de los fragmentos (AFLP`s) se utilizaron uno, tres y cinco discos FTA de 1.2 mm, cada disco con aproximadamente 25 ng de ADN. Los ensayos indicaron que los mejores productos de amplificación, para la visualización de AFLP′s, se obtuvieron de muestras con tres discos de FTA por individuo, lo que sugiere que con esta metodología, 75 ng de ADN por animal son suficientes para detectar polimorfismos de alta calidad en el genoma de Cavia porcellus. Se recomienda el uso de las tarjetas de FTA para el estudio genético de poblaciones de Cavia porcellus, con las modificaciones metodológicas descritas en este artículo para marcadores AFLP′s. A methodology that includes the use of FTA (Whatman Bioscience, Cambridge) to collect and store animals` blood samples and the procedures to extract and to get AFLP markers is presented in this paper. A review of the literature indicates that there are no reports concerning both aspects for the Cavia porcellus case. To reach our goal blood samples of three populations - Two native ones and other genetically improved- were obtained through heart puncture. This blood was stored in the FTA cards in order to extract, purify, amplify and analyze their DNA forms. All of the animals came from "Botana" farm of the Universidad de Nari o, located in Pasto, Colombia. For amplifying the AFLP one, three and five 1.2 mm FTA disks of approximately 75 ng of DNA per disk where used. The tests indicated that the best products to amplify and to visualize the AFLP where those ones obtained from samples of three FTA disks per animal. This suggests that 75 ng of DNA per animal is enough to generate AFLP of high quality in the Cavia porcellus` genome. We recom
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