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Search Results: 1 - 10 of 472073 matches for " Carla María Lourdes; Sepúlveda Tusek "
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Biotecnología para el desarrollo y sostenibilidad agroindustrial del Noroeste Argentino
Castagnaro,Atilio Pedro; Filippone,María Paula; Noguera,Aldo Sergio; Perera,María Francisca; Vellicce,Gabriel Ricardo; Mamaní,Alicia Inés; Ontivero,Marta Inés; Arias,Marta Eugenia; Racedo,Josefina; García,María Gabriela; Paz,Nora; Díaz,María Elena; Sendín,Lorena Noelia; Pardo,Esteban Mariano; Orce,Ingrid Georgina; Rocha,Carla María Lourdes; Sepúlveda Tusek,Mlena; Martínez Zamora,Gustavo; Salazar,Sergio Miguel; Dantur,Karina Inés; Rigano,Luciano; Torres,Pablo; Chalfoun,Nadia Regina; Mara?o,María Rosa; Vojnov,Adrián; Ploper,Leonardo Daniel; Díaz Ricci,Juan Carlos;
BAG. Journal of basic and applied genetics , 2011,
Abstract: in 1994, a group whose aims were both scientific research and technological development was set up. this team was inspired by the increasing demand of the agro-industrial sector in the north west of argentina. besides financial considerations inherent to the productive process, social and environmental issues were borne in mind when research had to be carried out. the conceptual premises of scientific work were the study, preservation and a judicious use of biodiversity; equally important was the relentless search for phytosanitary management alternatives which were friendly to both humans and the environment. thus, in 1997, the frst national program of strawberry genetic breeding in argentina started at insibio (conicet-unt), tucumán. this institute coordinated this program on a national level. thanks to an agreement between insibio and the estación experimental agroindustrial obispo colombres (eeaoc), in 2002, the eeaoc's biotechnology department, a unit associated to insibio, was founded. one of the main goals of this department was both to develop and adapt technologies to support institutional ongoing programs which intended not only to increase productivity but also to confer more sustainability to those agro-industries related to sugar and alcohol, soybean and citrus. and most importantly, a new scientific and technological team constituted by professionals working with other groups from different research centers nationwide carne into existence. their common goal has been the improvement of agro-industrial productive processes in order to help build a society which is fair and equitable for both the present and the future.
Evaluación de la variación somaclonal en vitroplantas de ca a de azúcar mediante marcadores moleculares Evaluation of somaclonal variation in in vitro produced sugarcane plants through molecular markers
María F. Perera,María G. García,Aldo S. Noguera,Milena Sepúlveda Tusek
Revista Industrial y Agrícola de Tucumán , 2010,
Abstract: El cultivo in vitro de tejidos vegetales puede producir variación somaclonal, fenómeno que consiste en modificaciones genéticas en las células y tejidos cultivados. Esto puede limitar la aplicación de dicha técnica para la micropopagación masiva, especialmente si la variación provoca un cambio fenotípico de importancia agronómica. En este trabajo se optimizó una metodología basada en la comparación de perfiles de marcadores moleculares AFLP (del inglés "Amplified Fragment Length Polymorphism"), para la detección de la variación somaclonal en vitroplantas de ca a de azúcar. Para la optimización de la técnica de AFLP en ca a de azúcar, se utilizaron plantas de seis genotipos propagados convencionalmente y dos tipos de muestras: hojas tiernas y meristemas. La variación somaclonal fue evaluada en líneas de vitroplantas de los mismos genotipos al final del cultivo in vitro, luego de seis meses de micropropagación. Con las 19 combinaciones de cebadores utilizadas, se diferenciaron los perfiles moleculares de los seis genotipos. En los plantines micropropagados se detectaron perfiles diferenciales en las variedades LCP85-384 y TUCCP77-42 con 3 de las 19 combinaciones de cebadores utilizadas. Este resultado muestra la validez de la técnica para detectar variantes somaclonales, y deja en evidencia la diferencia de susceptibilidad de los genotipos al cultivo in vitro. Esto permitió ajustar la metodología de micropropagación para cada genotipo multiplicado y asegurar la pureza genética de cada vitroplanta. In vitro culture of plant tissue can produce somaclonal variation, which consists of genetic modifications in cultured cells and tissues. This may constrain the use of this technique in massive micropopagation, especially if such change causes an agronomically relevant phenotypical modification. In this work, a methodology based on the comparison of AFLP (Amplified Fragment Length Polymorphism) molecular marker profiles was developed for detecting somaclonal variation in in vitro propagated sugarcane plants. To optimize AFLP technique application to sugarcane plants, six conventionally propagated genotypes and two types of samples (tender leaves and meristems) were used. Somaclonal variation was determined in micropropagated lines of these genotypes after six months of micropropagation. Molecular profile differentiation of the selected genotypes was achieved with 19 primer combinations. Differential profiles were detected in LCP85-384 and TUCCP77-42 micropropagated lines with 3 of the 19 primer combinations. This result demonstrated that the technique can be use
Evaluación de la variación somaclonal en vitroplantas de ca?a de azúcar mediante marcadores moleculares
Perera,María F.; García,María G.; Noguera,Aldo S.; Sepúlveda Tusek,Milena; Filippone,María P.; Castagnaro,Atilio P.;
Revista industrial y agr?-cola de Tucum??n , 2010,
Abstract: in vitro culture of plant tissue can produce somaclonal variation, which consists of genetic modifications in cultured cells and tissues. this may constrain the use of this technique in massive micropopagation, especially if such change causes an agronomically relevant phenotypical modification. in this work, a methodology based on the comparison of aflp (amplified fragment length polymorphism) molecular marker profiles was developed for detecting somaclonal variation in in vitro propagated sugarcane plants. to optimize aflp technique application to sugarcane plants, six conventionally propagated genotypes and two types of samples (tender leaves and meristems) were used. somaclonal variation was determined in micropropagated lines of these genotypes after six months of micropropagation. molecular profile differentiation of the selected genotypes was achieved with 19 primer combinations. differential profiles were detected in lcp85-384 and tuccp77-42 micropropagated lines with 3 of the 19 primer combinations. this result demonstrated that the technique can be used to detect somaclonal variants and that there are different susceptibility levels to in vitro culture among genotypes. therefore, micropropagation methodology was adjusted to each multiplied genotype so as to ensure genetic purity of in vitro propagated plants.
La pertinencia del Examen único de ingreso al bachillerato
Rivera Figueroa, Antonio;Guerrero Maga?a, María de Lourdes;Sepúlveda López, Armando;de Alaizola Arizmendi, I?aqui;
Perfiles educativos , 2006,
Abstract: is a multiple choice test, like that of the ceneval, the best form to select students for high school admission? in trying to respond this and other similar questions, we present in this paper the results of the analysis to the included mathematics and mathematical abilities items in the 2004 preparation guide to take the exani-i test. the preparation guide is available on the ceneval internet web page. in this paper, we also report the results of the application of these items to different students groups. the analysis of these results has shown that the exani-i test can be discriminating specific students, not because their lack of mathematical knowledge or their poor mathematical ability, but by another type of factors like the item and test design, or gender aspects.
La pertinencia del Examen único de ingreso al bachillerato
Antonio Rivera Figueroa,María de Lourdes Guerrero Maga?a,Armando Sepúlveda López,I?aqui de Alaizola Arizmendi
Perfiles educativos , 2006,
Abstract: Un examen de opción múltiple como el que elabora el CENEVAL, es la mejor forma de hacer la selección de los estudiantes al bachillerato? Tratando de responder a esta y otras preguntas, en este artículo presentamos los resultados de un análisis que realizamos a los reactivos de las áreas de Matemáticas y Habilidades Matemáticas de la Guía de examen del a o 2004, que sirve para preparar el EXANI-I y ha sido puesta a disposición del público general. Se reportan también los resultados obtenidos por diferentes grupos de estudiantes a los que fueron aplicados estos mismos reactivos. El análisis de estos resultados muestra que el EXANI-I puede estar discriminando a ciertos grupos de estudiantes, no por su falta de conocimiento matemático o limitada habilidad matemática, sino por otro tipo de factores, como lo es el dise o mismo de los reactivos.
The Importance of Affect in Teaching and Coursebooks for Young Learners in Chile
María Teresa Sepúlveda L.
Humanising Language Teaching , 2009,
Abstract:
Nutritional support in children under metabolic stress Soporte nutricional en el ni o con estrés metabólico
María Elsy Sepúlveda Hincapié
Iatreia , 1999,
Abstract: A review is presented on metabolic changes that occur in children under stress, and on the organs that are affected by the response to it; also considered are the different resources that are presently available in order to maintain nutrition in these children and to improve their probabilities of recovery. Se hace una revisión acerca de los cambios metabólicos que ocurren en el ni o durante el estrés y los órganos que se afectan en su respuesta; se tienen en cuenta además, los diferentes recursos que actualmente se pueden utilizar con el fin de mantener la nutrición del ni o durante este tipo de situación, mejorando sus posibilidades de recuperación.
Seminario Teoría, Historia y Crítica de la Traducción: El traductor como figura histórica
María Victoria Tipiani,Sebastián García Sepúlveda
Mutatis Mutandis : Revista Latinoamericana de Traducción , 2010,
Abstract:
Physical and rheological characterization of seeds of three legume trees
Estévez,Ana María; Escobar,Berta; Sepúlveda,Marcela;
Idesia (Arica) , 2012, DOI: 10.4067/S0718-34292012000100010
Abstract: the chilean algarrobo ("prosopis chilensis (mol) stuntz), the tara (caesalpinea spinosa (mol) kuntze) and the honey locust (gleditsia triacanthos l) have an interesting potential for the development of arid zones. the endosperm of the seeds of these species has a galactomannan-type gum which has the property of thickening and stabilizing emulsions. this study evaluates the physical characteristics of the pods and seeds, and the rheological properties of the gums of these species. we studied fruits of five ecotypes of algarrobo (coquimbo and metropolitan regions), of tara from the coquimbo region and of honey locust (three-spined acacia) from the metropolitan region. we measured the dimensions of the pods, number of seeds per pod, seed weight, gum yield and color parameters of the cotyledon and gum, and the rheological properties of the gums extracted. gums were extracted manually and dried at 50° c. the seeds of algarrobo weighed 0.04 g; their gum yield varied between 25.5% and 31.5%. the seeds of tara weighed 0.14 g and those of the acacia 0.16 g, with a gum yield of 31.0% and 40.1%, respectively. the gums had high luminosity and high contribution of yellow, along with low contribution of red. the dispersions of all the studied gums showed a non-newtonian herschel-bulkley behavior corresponding to an inelastic fluid, independent of time. the apparent viscosity of the gum of algarrobo fluctuated between 0.020 and 0.12; that of tara between 0.06 and 1.3, and that of honey locust between 0.034 and 0.08 pa*s. the gums of these species have rheological characteristics which would allow their addition to foods.
Variabilidad genética de bovinos Senepol en Colombia por marcadores moleculares Variabilidade genética de gado Senepol na Col mbia por marcadores moleculares Genetic variability of Senepol cattle in Colombia using molecular markers
Jeannie C Sepúlveda,Paula A ángel-Marín
Revista Colombiana de Ciencias Pecuarias , 2012,
Abstract: El ganado Senepol fue introducido en Colombia mediante el uso de la inseminación artificial y transferencia de embriones de un peque o núcleo de los animales. Objetivo: estimar la variabilidad genética del ganado Senepol de Colombia por medio de marcadores microsatélites y estimar las frecuencias alélicas y genotípicas de SNPs en los genes que codifican para la calpastatina (CAST1), calpaína (CALP316) y leptina (PB). Métodos: 412 muestras de sangre de animales pertenecientes a 28 fincas fueron analizados para los STRs: INRA32, BM2113, ETH10, BM1824, INRA037, ETH225, INRA064, SPS115, TGLA126 y TGLA122 y los tres SNPs. Resultados: los microsatélites y los SNPs fueron polimórficos. El número de alelos de los microsatélites variaron entre 4 (BM1824) y 11 (INRA37), la heterocigosidad observada varió entre 0.21 (INRA64) y 0.89 (BM2113). La probabilidad de exclusión para el total de microsatélites fue mayor que 99.99%, indicando que el conjunto de microsatélites pueden ser usados para pruebas de filiación. Conclusiones: a pesar de ser una población peque a y cerrada, este núcleo presenta una alta variabilidad genética y baja consanguinidad. O gado Senepol foi introduzido na Col mbia mediante o uso da insemina o artificial e a transferência de embri es de um núcleo pequeno de animais. Objetivo: estimar a variabilidade genética do gado Senepol da Col mbia mediante marcadores microsatélites e estimar as frequências alélicas e genotípicas dos SNPs dos genes de calpastatina (CAST1), calpaina (CALP316) e leptina (PB). Métodos: 412 amostras de sangue de animais pertencentes a 28 rebanhos foram analisadas para os STRs INRA32, BM2113, ETH10, BM1824, INRA037, ETH225, INRA064, SPS115, TGLA126 e TGLA122 e os três SNPs. Resultados: os microsatélites e os SNPs foram polimórficos. O número de alelos dos microsatélites variaram entre 4 (BM1824) e 11 (INRA37), a heterocigosidade observada variou entre 0,21 (INRA64) e 0,89 (BM2113). A probabilidade de exclus o para o total de microsatélites polimórficos foi maior que 99.99%, indicando que o conjunto de microsatélites podem ser usados para testes de filia o. Conclus es: embora seja uma popula o pequena e fechada, o núcleo apresenta uma alta variabilidade genética e baixa consanguinidade. The Senepol beef cattle breed was introduced into Colombia through the use of artificial insemination and embryo transfer from a small nucleus of animals. Objective: to estimate the genetic variability of Senepol cattle in Colombia by heterologous microsatellites and to estimate gene and genotypic frequencies of single nucleotide polymorphic
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