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Search Results: 1 - 10 of 221382 matches for " Campos Carlos Augusto de Martino "
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Detection of Mycoplasma pulmonis in laboratory rats
Barreto Maria Lucia,Nascimento Elmiro Rosendo do,Campos Carlos Augusto de Martino,Nascimento Maria da Gra?a Fichel do
Brazilian Journal of Microbiology , 2002,
Abstract: This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises. The ELISA test was carried out in 20 animals of both colonies. In the conventional controlled premises, 92.86% (13/14) were positive for M. pulmonis, and 7.14% (1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. The results confirmed that M. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the ELISA positivity rated higher than culture.
Detec??o do Grupo Mycoplasma mycoides por imunoperoxidase indireta (IPI) e PCR-REA em conduto auditivo de bovinos
Santos, Sandra Batista dos;Nascimento, Elmiro Rosendo do;Faccini, Jo?o Luiz Horacio;Barreto, Maria Lúcia;Almeida, Juliana Ferreira de;Pereira, Virginia Léo de Almeida;Campos, Carlos Augusto Martino;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000500015
Abstract: mycoplasma mycoides cluster (mmc) was diagnosed by polimerase chain reaction-restriction endonuclease analysis (pcr-rea) and indirect immunoperoxidase (ipi), both, carried out in flushing from external ear canal, collected from bovine at slaughter time in the state of rio de janeiro, southeastern, brazil. a total of 60 bovines were randomly selected. sterile syringes (60ml) loaded with buffer solution (pbs, ph 7.2) were used for the ear canal flushing. the obtained samples were stored in glycerol (1:2) and frozen at -20oc until use. these specimens were diluted up to 10-5, inoculated in liquid and solid modified hayflick′s media and incubated at 37oc for 2-3 days. the plates were kept in a microaerophilia condition and examined every two days under a stereomicroscope for the presence of typical colonies “fried-egg”. in this study, 35 strains selected in agreement with their biochemistry and physiologic proprieties, were used. from the 60 cultivated samples, 48 (80.00%) were positive for mycoplasma spp. under ipi the prevalence obtained for mmc was 20.0% (12/60) while by pcr-rea it was 41.7% (25/60). the ipi typing of these isolates resulted in 58.3% (7/12) for m.mycoides mycoides lc and 41.7% (5/12) for m. capricolum. pcr-rea for mmc was confirmed by the amplicon size of 785bp, compatible with this group. the kappa value for the association between these two tests was 0.14 (p>0.05). after restriction analysis with alui in all mmc strains the fragments size obtained were of 81, 98, 186 and 236bp, but not of 370bp that is compatible with mycoides mycoides mycoides sc of bovine type. the presence of mycoplasmas species in the ear canal of asymptomatic bovines represent a risk of subsequent propagation of mycoplasma spp. among bovine herds in brazil.
Detection of Mycoplasma pulmonis in laboratory rats
Barreto, Maria Lucia;Nascimento, Elmiro Rosendo do;Campos, Carlos Augusto de Martino;Nascimento, Maria da Gra?a Fichel do;Lignon, Gilberto Brasil;Lira, Marie Luce Flores;Silva, Ricardo G.;
Brazilian Journal of Microbiology , 2002, DOI: 10.1590/S1517-83822002000300015
Abstract: this work was conducted on rats in two premises located in niterói and rio de janeiro, brazil. one is classified as conventional controlled and the other, conventional. the objective of the present study was to detect the presence of mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. in the conventional controlled premises, 16 rats of wistar-furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of hooded lister strain. the clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. from 28 rats, 57.14% (16/28) were culture positive for m. pulmonis, being 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises. the elisa test was carried out in 20 animals of both colonies. in the conventional controlled premises, 92.86% (13/14) were positive for m. pulmonis, and 7.14% (1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. the results confirmed that m. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the elisa positivity rated higher than culture.
Desenvolvimento de mudas de citros cultivadas em vaso em resposta à aduba??o NPK
Bernardi, Alberto Carlos de Campos;Carmello, Quirino Augusto de Camargo;Carvalho, Sérgio Alves de;
Scientia Agricola , 2000, DOI: 10.1590/S0103-90162000000400022
Abstract: the objective of this research was to evaluate the npk fertilizer effect on 'valencia' sweet orange nursery tree (citrus sinensis) development, budded on rangpur lime rootstocks (c. limonia) in a protected environment using containers with pinus bark, vermiculite and perlite substrates. the experiment consisted of a complete (1/5) 53 factorial randomized block design. treatments comprised five concentrations in g per plant of n: 1.25; 6.25; 11.25; 16.25; 21.25; five concnetrations of k: 0.42; 3.75; 6.22; 9.34; 12.45; and five concentration of p: 0.19; 0.89; 1.59; 2.29; 2.99. nitrogen and k were applieded weekly, and 37.5% of the total fertilization was applied to rootstocks. all p was supplied at planting. leaf, root, stem, shoot and total dry matter production, shoot/root ratio, leaf area, plant height, root system volume; leaf area dry matter ratio stem diameter were evaluated. response functions were adjusted and nutrient rates for maximum yield are presented. results showed that fertilizer level that lead to the best 'valencia' sweet orange on rangpur lime nursery tree response was (g per plant): n, 9.85; p, 2.86; k, 7.99.
Macronutrientes em mudas de citros cultivadas em vasos em resposta à aduba??o NPK
Bernardi, Alberto Carlos de Campos;Carmello, Quirino Augusto de Camargo;Carvalho, Sérgio Alves de;
Scientia Agricola , 2000, DOI: 10.1590/S0103-90162000000400026
Abstract: 'valencia' sweet orange nursery trees (citrus sinensis) budded on rangpur lime rootstocks (c. limonia) were grown in containers using pine bark, vermiculite and perlite as substracts with the objective of evaluating the npk fertilization effect on macronutrient levels. the experiment was a complete (1/5) 53 factorial randomized block design. treatments comprised five concentrations in g per plant of n: 1.25; 6.25; 11.25; 16.25; 21.25; five concnetrations of k: 0.42; 3.75; 6.22; 9.34; 12.45; and five concentration of p: 0.19; 0.89; 1.59; 2.29; 2.99. nitrogen and k were applieded weekly, and 37.5% of the total fertilization was applied to rootstocks. all p was supplied at planting. young and old leaves, root and stem total nutrient concentrations of n, p, k, ca, mg, s and npk accumulation were evaluated. tissue n concentration was directly and p and k concentrations were inversely related to nitrogen fertilization. calcium, mg and s concentrations increased up to the intermediate n fertilization rates. k fertilizer inhibited ca and mg absorption by plants. rootstock npk accumulated as 30% in roots and 70% in shoot.
Macronutrientes em mudas de citros cultivadas em vasos em resposta à aduba o NPK
Bernardi Alberto Carlos de Campos,Carmello Quirino Augusto de Camargo,Carvalho Sérgio Alves de
Scientia Agricola , 2000,
Abstract: Mudas de laranjeira 'Valência' (Citrus sinensis) sobre o porta-enxerto de limoeiro 'Cravo' (C. limonia) foram cultivadas em vasos com substrato de casca de Pinus, vermiculita e perlita com o objetivo de avaliar-se os efeitos do fornecimento de N, P e K sobre os teores de macronutrientes do porta-enxerto e das mudas. Utilizou-se um esquema de fatorial fracionário (1/5)53 com um delineamento em blocos ao acaso. Os tratamentos consistiram em 5 doses (em g por planta) de N: 1,25; 6,25; 11,25; 16,25; 21,25; 5 doses de K: 0,42; 3,75; 6,22; 9,34; 12,45; e 5 doses de P: 0,19; 0,89; 1,59; 2,29; 2,99. O N e K foram fornecidos semanalmente, sendo 37,5% da dose para o porta-enxerto. O P foi fornecido totalmente no plantio. Determinaram-se os teores totais de N, P e K, Ca, Mg e S nas folhas novas e velhas, raízes e caule e a extra o de N, P e K. Os teores de N relacionaram-se diretamente e os de P e K inversamente com a aduba o nitrogenada. Os teores de Ca, Mg e S relacionaram-se positivamente até as doses intermediárias de N utilizadas. Houve efeito inibitório do fertilizante potássico sobre a absor o de Ca e Mg. A acumula o de NPK pelos porta-enxertos foi em torno de 30% pelas raízes e 70% pela parte aérea.
Desenvolvimento de mudas de citros cultivadas em vaso em resposta à aduba o NPK
Bernardi Alberto Carlos de Campos,Carmello Quirino Augusto de Camargo,Carvalho Sérgio Alves de
Scientia Agricola , 2000,
Abstract: O objetivo deste trabalho foi avaliar o efeito da aduba o NPK sobre o desenvolvimento de mudas de laranjeira 'Valência' (Citrus sinensis) sobre o porta-enxerto de limoeiro 'Cravo' (C. limonia) em vasos com substrato de casca de Pinus, vermiculita e perlita. Utilizou-se um esquema de fatorial fracionário (1/5)53 com um delineamento em blocos ao acaso. Os tratamentos consistiram em 5 doses (em g por planta) de N: 1,25; 6,25; 11,25; 16,25; 21,25; 5 doses de K: 0,42; 3,75; 6,22; 9,34; 12,45; e 5 doses de P: 0,19; 0,89; 1,59; 2,29; 2,99. O N e o K foram fornecidos semanalmente, sendo 37,5% da dose para o porta-enxerto. O P foi fornecido totalmente no plantio. Avaliaram-se a produ o de material seco das folhas, raízes, caule, parte aérea e total; área foliar, rela o raízes/parte aérea; altura de plantas; volume radicular, rela o área foliar/material seco para os porta-enxertos e as mudas, e o diametro do caule para os porta-enxertos. Fun es de resposta foram ajustadas e os níveis de nutrientes foram calculados. Os resultados mostraram que as doses de fertilizantes que levaram às melhores respostas para produ o de mudas de laranjeira 'Valência'/'Cravo' sobre o porta-enxertos de limoeiro 'Cravo' foram: N, 9,85; P, 2,86; K, 7,99 g por plantas.
The growth dynamics in Brachiaria species according to nitrogen dose and shade
Paciullo, Domingos Sávio Campos;Fernandes, Priscila Beligoli;Gomide, Carlos Augusto de Miranda;Castro, Carlos Renato Tavares de;Sobrinho, Fausto de Souza;Carvalho, Carlos Augusto Brand?o de;
Revista Brasileira de Zootecnia , 2011, DOI: 10.1590/S1516-35982011000200006
Abstract: it was studied the effects of shading (0, 36 and 54%) and of four nitrogen doses (0, 50, 100 and 150 mg n/dm3 of soil) on the morphogenetic traits and on the tillering of grasses of brachiaria genus (b. decumbens, b. ruziziensis and b. brizantha cv. marandu and cv. xaraes), cultivated in pots with 5 kg substrate. it was used a complete random design, in a 3 × 4 × 4 factorial scheme with three replications. nitrogen promoted increase in leaf appearance rate and in average leaf blade length, whereas shading increased stem elongation rate. leaf elongation rate and tillering varied with the interaction among shading percentages and nitrogen doses. shading increased response efficiency of leaf elongation rate to nitrogen doses, but reduced for tillering, evidencing priority existing tillering growth in detriment to appearance of new tillers when there is light restriction for the plants. phenotypic adjustments, in response to light reduction, indicate that the studied grasses present tolerance to moderate shading.
Associa??o de fases meióticas e estádios dos micrósporos com características morfológicas de bot?es florais de piment?o
Picoli, Edgard Augusto de Toledo;Carvalho, Carlos Roberto de;Fári, Miklos;Otoni, Wagner Campos;
Ciência e Agrotecnologia , 2003, DOI: 10.1590/S1413-70542003000300029
Abstract: in the present study, morphological characters adopted for floral bud selection used for androgenesis induction were associated with pepper (capsicum annuum l. cv. azeth) meiotic phases and microspore stages. floral buds were harvested from greenhouse-grown plants and separated into six classes according to size relationships between calyx and corolla, and anthocyanin pigmentation in anthers. after sorting by size, buds were fixed using a methanol: acetic acid (3:1) solution at -20oc. cytogenetic preparations were mounted using an adaptation of an air-drying technique and staining through a phosphate buffer giemsa solution. bud observations were accomplished under a stereo-microscope and the cytogenetic preparations in an optic microscope. recordings of floral buds and meiotic phases were performed with an image digitizing computer system. variations on the meiotic phases were observed within each class. although the approach based on anthocyanin pigmentation on anthers has been applied to another pepper varieties, it was not a reliable trait for determination of the microspore stage. meiotic phases were citogeneticaly identified, although bud stage could not be associated with it. on the other hand, buds having the same calyx and corolla sizes presented a larger number of microspores in a suitable stage for anther culture.
ASSESSMENT OF IVERMECTIN THERAPEUTIC EFFICACY ON THIRD-STAGE LARVAE OF Lagochilascaris minor IN MICE EXPERIMENTALLY INFECTED
BARBOSA, Carlos Augusto Lopes;CAMPOS, Dulcinéa Maria Barbosa;OLIVEIRA, Jayrson Araújo de;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1998, DOI: 10.1590/S0036-46651998000300002
Abstract: in this study we evaluated the potential action of ivermectin on third-stage larvae, both at migratory and encysted phases, in mouse tissues after experimental infection with lagochilascaris minor. study groups i and ii consisted of 120 mice that were orally administered 1,000 parasite eggs. in order to assess ivermectin action upon migratory larvae, group i (60 mice) was equally split in three subgroups, namely i-a, i-b, and i-c. on the 7th day after inoculation (dai), each animal from the subgroup i-a was treated with 200 μg/kg ivermectin while subgroup i-b was given 1,000 μg/kg, both groups received a single subcutaneous dose. to assess the drug action on encysted larvae, group ii was equally split in three subgroups, namely ii-a, ii-b, ii-c. on the 45th dai each animal was treated with ivermectin at 200 μg/kg (subgroup ii-a) and 1,000 μg/kg (group ii-b) with a single subcutaneous dose. untreated animals of subgroups i-c and ii-c were used as controls. on the 60th dai all animals were submitted to larva search. at a dose of 1,000 μg/kg the drug had 99.5% effectiveness on third-stage migratory larvae (subgroup i-b). ivermectin efficacy was lower than 5% on third-stage encysted larvae for both doses as well as for migratory larvae treated with 200μg/kg.
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