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Search Results: 1 - 10 of 54527 matches for " CUI Feng-jie "
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A novel bacteriophage KSL-1 of 2-Keto-gluconic acid producer Pseudomonas fluorescens K1005: isolation, characterization and its remedial action
Wen-Jing Sun, Chang-Feng Liu, Lin Yu, Feng-jie Cui, Qiang Zhou, Si-lian Yu, Lei Sun
BMC Microbiology , 2012, DOI: 10.1186/1471-2180-12-127
Abstract: The phage KSL-1 of Pseudomonas fluorescens K1005 was isolated from abnormal 2KGA fermentation broth. It belonged to the Siphoviridae family with a hexagonal head diameter of about 99?nm and a non-contractile tail of about 103?nm?×?39?nm. The genome size of phage KSL-1 was estimated to be approximately 53 kbp. Its optimal MOI to infect P. fluorescens K1005 was about 0.001. One-step growth curve gave its latent and burst periods of 90?min and 75?min with a burst size of 52 phage particles per infected cell. This phage was stable with a pH range of 7.0–10.0, and sensitive to thermal treatment. Finally, a simple remedial action was proposed by feeding fresh seed culture. Compared with the infected 2KGA fermentation, the remedial experiments restored 2KGA fermentation performance by increasing the produced 2KGA concentration to 159.89?g/L and shortening the total fermentation time of 80?h with the productivity and yield of 2.0?g/L.h and 0.89?g/g. The obtained data proved that this method was effective to combat the phage infections problems during the 2KGA fermentation.The phage KSL-1 was a novel bacteriophage specifically infecting Pseudomonas fluorescens K1005. The remedial action of feeding fresh seed culture to the infected broth was an easily-operating and effective method to maintain a high 2KGA yield and avoid the draft of infected broth.
Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
Da-Ming Huang,Tian-Zhen Zhang,Feng-Jie Cui,Wen-Jing Sun,Li-Ming Zhao,Meng-Yi Yang,Ya-Juan Wang
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/917232
Abstract: A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were set as follows: detection wavelength of 280 nm, mobile phase of water and methanol gradient elution, temperature for the chromatographic column of 30°C, flow rate of mobile phase of 0.8 mL/min, sample injection volume of 5 μL, and elution time of 40 min. The MS conditions were set as follows: the flow rate of sheath gas, aux gas, and sweep gas were kept at 35 arb, 5 arb, and 0 arb, respectively. The temperature of capillary was held at 300°C, and capillary voltage was set at 30.00 V. Tube lens were performed at 100.00 V. The proposed method was validated by linearity (2 ≥ 0.9910), average recovery (94.93%, RSD1.21%), precision (RSD ≤ 1.31%), limit of detection, and limit of quantification (LOD 0.1~0.12 mg/L, LOQ 0.5~0.67 mg/L), which proved to be affordable for simultaneously determining canrenone and its bio-transformed product 11-α-hydroxy-canrenone.
Rapid differentiation and identification of five species of Salmonella by FT-IR spectroscopy
傅立叶变换红外光谱技术对5种沙门氏菌的快速分类鉴定

YANG Li-jun,LI Zhao-jie,SONG Xiao-hu,WANG Jing,LIU Yu-min,CUI Feng-jie,
杨丽君
,李兆杰,宋晓华,王静,刘玉敏,崔凤杰

微生物学通报 , 2013,
Abstract: Objective] To establish a standard fourier transform infrared (FT-IR) spectral library and a FT-IR method of differentiation and identification of five species of Salmonella, viz Salmonella typhimurium, Salmonella enteritidis, Salmonella choleraesuis, Salmonella arizonae and Salmonella potsdam. Methods] FT-IR fingerprint absorption spectra of five species of Salmonella were collected and analyzed by chemometric methods. Results] A standard FT-IR spectral library was created and could be used to compare with those of the target Salmonella and identify them; two cluster models of principal component analysis (PCA) and hierarchical cluster analysis (HCA) were established and could differentiate and identify well the five species of Salmonella. Conclusion] As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy is an effective tool to differentiate and identify five species of Salmonella studied here.
Comparisons of regulating blood glucose effects of Ginkgo biloba leaf extract with and without biotransformation by Hericium erinaceus
银杏叶提取物猴头菌转化前后降血糖作用的比较研究

ZHANG Zhi-Cai,LIAN Bin,CUI Feng-Jie,HUANG Da-Ming,CHANG Wei-Zhong,
张志才
,连宾,崔凤杰,黄达明,常为众

微生物学报 , 2008,
Abstract: The regulatory effects of blood glucose, lipid metabolism and free radical elimination were compared in streptozotocin-induced hyperglycemic rats among the following treatments: Ginkgo biloba leaf extract (EGB), the biotransformation of EGB by Hericium erinaceus, a cultural filtrate of H.erinaceus, and the cultural filtrate of H.erinaceus plus EGB, together with the normal control, the model control and the positive control (Metformin). The best results were obtained from the biotransformation treatment group, which could significantly reduce the levels of blood glucose and fructosamine. However, the treatment did not increase the blood insulin level. The EGB transformed products could obviously increase the serum superoxide dismutase activity and reduce the malondialdehyde level, but the reduction of malondialdehyde was not obvious as compared with that of the other treatment groups. This study provides a useful information on improving the medical properties of the herb extracts by biotransformation.
FIELD COLLECTED PLANT SPECTRUM DENOISING BY LOGARITHM TRANSFORM AND WAVELET TRANSFORM
对数变换与小波变换用于野外采集植物波谱降噪

ZHOU Guang-Zhu,WANG Cui-Zhen,YANG Feng-Jie,LI Yin-Ming,
周广柱
,王翠珍,杨锋杰,李寅明

红外与毫米波学报 , 2009,
Abstract: The objects' spectrum is often contaminated by noise when it is collected in the open air.According to the principle of the spectrum collection,the noise was considered as one kind of multiplicative compound noise.By theoretical derivation,the combination of logarithm transform and wavelet transform was introduced into noise reduction.Multiplicative noise simulation test was carried out.And the results show that the spatial correlation algorithm is best suited for spectral data denoising,modulus maxima algo...
Comparisons of regulating blood glucose effects of Ginkgo biloba leaf extract with and without biotransformation by Hericium erinaceus
银杏叶提取物猴头菌转化前后降血糖作用的比较研究

ZHANG Zhi-Cai,LIAN Bin,CUI Feng-Jie,HUANG Da-Ming,CHANG Wei-Zhong,
张志才
,连宾,崔凤杰,黄达明,常为众

菌物学报 , 2008,
Abstract: The regulatory effects of blood glucose, lipid metabolism and free radical elimination were compared in streptozotocin-induced hyperglycemic rats among the following treatments: Ginkgo biloba leaf extract (EGB), the biotransformation of EGB by Hericium erinaceus, a cultural filtrate of H.erinaceus, and the cultural filtrate of H.erinaceus plus EGB, together with the normal control, the model control and the positive control (Metformin). The best results were obtained from the biotransformation treatment group, which could significantly reduce the levels of blood glucose and fructosamine. However, the treatment did not increase the blood insulin level. The EGB transformed products could obviously increase the serum superoxide dismutase activity and reduce the malondialdehyde level, but the reduction of malondialdehyde was not obvious as compared with that of the other treatment groups. This study provides a useful information on improving the medical properties of the herb extracts by biotransformation.
Evolutionary Patterns in the Sequence and Structure of Transfer RNA: Early Origins of Archaea and Viruses
Feng-Jie Sun,Gustavo Caetano-Anollés
PLOS Computational Biology , 2008, DOI: 10.1371/journal.pcbi.1000018
Abstract: Transfer RNAs (tRNAs) are ancient molecules that are central to translation. Since they probably carry evolutionary signatures that were left behind when the living world diversified, we reconstructed phylogenies directly from the sequence and structure of tRNA using well-established phylogenetic methods. The trees placed tRNAs with long variable arms charging Sec, Tyr, Ser, and Leu consistently at the base of the rooted phylogenies, but failed to reveal groupings that would indicate clear evolutionary links to organismal origin or molecular functions. In order to uncover evolutionary patterns in the trees, we forced tRNAs into monophyletic groups using constraint analyses to generate timelines of organismal diversification and test competing evolutionary hypotheses. Remarkably, organismal timelines showed Archaea was the most ancestral superkingdom, followed by viruses, then superkingdoms Eukarya and Bacteria, in that order, supporting conclusions from recent phylogenomic studies of protein architecture. Strikingly, constraint analyses showed that the origin of viruses was not only ancient, but was linked to Archaea. Our findings have important implications. They support the notion that the archaeal lineage was very ancient, resulted in the first organismal divide, and predated diversification of tRNA function and specificity. Results are also consistent with the concept that viruses contributed to the development of the DNA replication machinery during the early diversification of the living world.
Evolutionary Patterns in the Sequence and Structure of Transfer RNA: A Window into Early Translation and the Genetic Code
Feng-Jie Sun, Gustavo Caetano-Anollés
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002799
Abstract: Transfer RNA (tRNA) molecules play vital roles during protein synthesis. Their acceptor arms are aminoacylated with specific amino acid residues while their anticodons delimit codon specificity. The history of these two functions has been generally linked in evolutionary studies of the genetic code. However, these functions could have been differentially recruited as evolutionary signatures were left embedded in tRNA molecules. Here we built phylogenies derived from the sequence and structure of tRNA, we forced taxa into monophyletic groups using constraint analyses, tested competing evolutionary hypotheses, and generated timelines of amino acid charging and codon discovery. Charging of Sec, Tyr, Ser and Leu appeared ancient, while specificities related to Asn, Met, and Arg were derived. The timelines also uncovered an early role of the second and then first codon bases, identified codons for Ala and Pro as the most ancient, and revealed important evolutionary take-overs related to the loss of the long variable arm in tRNA. The lack of correlation between ancestries of amino acid charging and encoding indicated that the separate discoveries of these functions reflected independent histories of recruitment. These histories were probably curbed by co-options and important take-overs during early diversification of the living world.
The ancient history of the structure of ribonuclease P and the early origins of Archaea
Feng-Jie Sun, Gustavo Caetano-Anollés
BMC Bioinformatics , 2010, DOI: 10.1186/1471-2105-11-153
Abstract: To study the evolution of this complex, we constructed rooted phylogenetic trees of RPR molecules and substructures and estimated RPP age using a cladistic method that embeds structure directly into phylogenetic analysis. The general approach was used previously to study the evolution of tRNA, SINE RNA and 5S rRNA, the origins of metabolism, and the evolution and complexity of the protein world, and revealed here remarkable evolutionary patterns. Trees of molecules uncovered the tripartite nature of life and the early origin of archaeal RPRs. Trees of substructures showed molecules originated in stem P12 and were accessorized with a catalytic P1-P4 core structure before the first substructure was lost in Archaea. This core currently interacts with RPPs and ancient segments of the tRNA molecule. Finally, a census of protein domain structure in hundreds of genomes established RPPs appeared after the rise of metabolic enzymes at the onset of the protein world.The study provides a detailed account of the history and early diversification of a fundamental ribonucleoprotein and offers further evidence in support of the existence of a tripartite organismal world that originated by the segregation of archaeal lineages from an ancient community of primordial organisms.With few exceptions [1], ribonuclease P (RNase P) is one of two universal ribozymes (the other is the ribosome) that are present in all living organisms. This ribonucleoprotein is generally composed of an RNA subunit, the RNase P RNA (RPR), and one or more protein subunits, the RNase P proteins (RPPs) [2]. RNase P functions as a phosphodiesterase carrying out the 5' endonucleolytic cleavage of transfer RNA (tRNA) precursor transcripts (pre-tRNA) to form mature functional tRNAs [3-5]. Regions of the RPR that contribute to the recognition of the substrate cleavage sites [the tRNA pseudouridine (TΨC) loop and CCA tail] are well studied. Remarkably, the catalytic function can be conducted by the RNA subunit indepen
Differentiation of two strains of Escherichia coli by FT-IR spectroscopy
傅立叶变换红外光谱技术对两株大肠杆菌的鉴别

WANG Jing,YANG Li-ju,LI Zhao-jie,LIU Yu-min,SHI Wen-chun,CUI Feng-jie,SONG Xiao-hua,CONG Wei-hong,
王静
,杨丽君,李兆杰,刘玉敏,时文春,崔凤杰,宋晓华,丛伟红

微生物学通报 , 2012,
Abstract: Objective] To differentiate two strains of Escherichia coli from different sources by fourier transform infrared spectroscopy (FT-IR). Methods] FT-IR fingerprint absorption spectra of two strains of E. coli from different sources were collected and analyzed by chemometric methods. Results] Two cluster models of principal component analysis (PCA) and hierarchical cluster analysis (HCA) were established and could differentiate well the two strains of E. coli. Conclusion] As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy can be used to differentiate microorganisms at the strain level from different sources.
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