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Search Results: 1 - 10 of 19470 matches for " CRISTINA VALLETA; CHADA BARACAT "
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Genetic polymorphism of GSTM1 in women with breast cancer and interact with reproductive history and several clinical pathologies
Biological Research , 2005, DOI: 10.4067/S0716-97602005000200017
Abstract: due to the conflicting results regarding the association between breast cancer and the gstm1 null mutation, our aim was to research this association in a brazilian population and correlations with smoking, reproductive history and several clinical pathologies. a case-control study was performed on 105 women with breast cancer and 278 controls. extraction of dna was accomplished according to the protocol of the gfx? kit and polymorphism analysis by the pcr technique. the control and experimental groups were compared and statistical analysis assessed by x2 or fisher's exact test. the deletion in the gstm1 gene in the breast cancer group had a prevalence of 32 (30.4%) individuals with the presence of null mutation. in the control group, the null mutation was present in 104 (37.4%) women. upon comparison of the two groups, no statistically significant difference of the gstm1 gene was observed, with an odds ratio (or) of 0.74, 95%, confidence interval (ci) 0.45 - 1.20, p = 0.277. the results conclusively show that single gene gstm1 polymorphisms do not confer a substantial risk of breast cancer to its carriers. furthermore, in this study no correlation was found between gsts and smoking, reproductive history and several clinical pathologies with respect to cancer risk.
A??o da melatonina sobre a apoptose e fator de crescimento endotelial vascular no córtex da adrenal de ratas pinealectomizadas
Fuchs, Luiz Fernando Portugal;Guimar?es, Camila Rennó;Maganhin, Carla Cristina;Sim?es, Ricardo Santos;Baracat, Maria Candida Pinheiro;Soares Júnior, José Maria;Baracat, Edmund Chada;
Revista Brasileira de Ginecologia e Obstetrícia , 2010, DOI: 10.1590/S0100-72032010000800003
Abstract: purpose: to evaluate the reactivity of vegf-a and cleaved caspase-3 in the adrenal gland cortex of female pinealectomized rats treated with melatonin. methods: forty adult female rats were divided into 4 groups (g) of 10 animals: gi - no surgical intervention, with vehicle administration; gii - sham pinealectomized with vehicle administration; giii - pinealectomized with vehicle administration; giv - pinealectomized with melatonin administration (10 μg/animal) during the night. after 60 days of treatment, all animals were anesthetized, and the adrenal glands were removed and fixed in 10% formaldehyde (phosphate buffered) for histological processing and paraffin embedding. sections (5 μm thick) were collected on silanized slides and submitted to imunnohistochemical methods for the detection of cleaved caspase-3 (apoptosis) and of vascular endothelial growth factor (vegf-a) in the adrenal cortex. the data obtained were submitted to analysis of variance (anova) complemented by the tukey-kramer test (p<0.05). results: reactivity to cleaved caspase-3 was noted in the zona glomerulosa of the adrenal glands in all studied groups. there were no significant differences in the zona glomerulosa; however, the zona fasciculata (15.51±3.12*, p<0.05) and the zona reticularis (8.11±1.90*, p<0.05) presented the smallest percentage of apoptosis in the pinealectomized group (giii). the reactivity to the vegf-a was stronger in the zona glomerulosa and weaker in the zona reticularis in all groups. we found a stronger vegf-a reactivity in the zona fasciculata in the pinealectomized group (giii). conclusions: the pineal gland affects the arrangement of the zona glomerulosa and reticularis of the adrenal glands, which are related to the production of sex hormones.
A comparison of CA125, HE4, risk ovarian malignancy algorithm (ROMA), and risk malignancy index (RMI) for the classification of ovarian masses
Anton, Cristina;Carvalho, Filomena Marino;Oliveira, Elci Isabel;Maciel, Gustavo Arantes Rosa;Baracat, Edmund Chada;Carvalho, Jesus Paula;
Clinics , 2012, DOI: 10.6061/clinics/2012(05)06
Abstract: objective: differentiation between benign and malignant ovarian neoplasms is essential for creating a system for patient referrals. therefore, the contributions of the tumor markers ca125 and human epididymis protein 4 (he4) as well as the risk ovarian malignancy algorithm (roma) and risk malignancy index (rmi) values were considered individually and in combination to evaluate their utility for establishing this type of patient referral system. methods: patients who had been diagnosed with ovarian masses through imaging analyses (n = 128) were assessed for their expression of the tumor markers ca125 and he4. the roma and rmi values were also determined. the sensitivity and specificity of each parameter were calculated using receiver operating characteristic curves according to the area under the curve (auc) for each method. results: the sensitivities associated with the ability of ca125, he4, roma, or rmi to distinguish between malignant versus benign ovarian masses were 70.4%, 79.6%, 74.1%, and 63%, respectively. among carcinomas, the sensitivities of ca125, he4, roma (pre-and post-menopausal), and rmi were 93.5%, 87.1%, 80%, 95.2%, and 87.1%, respectively. the most accurate numerical values were obtained with rmi, although the four parameters were shown to be statistically equivalent. conclusion: there were no differences in accuracy between ca125, he4, roma, and rmi for differentiating between types of ovarian masses. rmi had the lowest sensitivity but was the most numerically accurate method. he4 demonstrated the best overall sensitivity for the evaluation of malignant ovarian tumors and the differential diagnosis of endometriosis. all of the parameters demonstrated increased sensitivity when tumors with low malignancy potential were considered low-risk, which may be used as an acceptable assessment method for referring patients to reference centers.
Efeitos da isoflavona e dos estrogênios conjugados eqüinos sobre a qualidade de vida na pós-menopausa
Vieira, Lúcia Helena Laprano;Soares Júnior, José Maria;Nunes, Márcia Gaspar;Sim?es, Ricardo Santos;Kaari, Cristina;Haidar, Mauro Abi;Baracat, Edmund Chada;
Revista Brasileira de Ginecologia e Obstetrícia , 2007, DOI: 10.1590/S0100-72032007000500005
Abstract: purpose: to analyze the isoflavone and estrogen effects on the postmenopausal quality of life. methods: this is a randomized and double-blind study with 79 postmenopausal patients, 12 months of amenorrhea, 40 years old or more and body mass index (bmi) above 30 kg/m2. the participants were randomly divided into two treatment groups: gecp received orally two capsules, every 12 hours, one contained 0.625 mg conjugated equine estrogen and another placebo (n=33); gecs received two capsules of 150 mg extract of soy, with 60 mg isoflavone (n=32). both treatments were administered for six months. the quality menopause specific questionnaire of life was applied before and after one, three and six months of treatment. the parameters of gynecological cancer risk were evaluated. anova and the tukey test were used for data analysis. results: there was a reduction in the values of the vasomotor parameters after six months of treatment, 1.6±0.8 and 2.4±1.6, compared to before therapy, 4.0±2.2 and 4.2±2.3 in gecp and gecs, respectively. the psychological aspects showed reduction in values after six months of therapy, 2.5±1.2 and 2.9±1.4, compared to before treatment, 3.6±1.6 and 4.1±1.9 in gecp and gecs, respectively. similar results were obtained on the physical aspects and in the sexual symptoms. conclusions: isoflavones may positively act on life quality of postmenopausal women. this effect was similar to conjugated equine estrogen.
Melatonina: modulador de morte celular
Ferreira, Cecília da Silva;Maganhin, Carla Cristina;Sim?es, Ricardo dos Santos;Gir?o, Manoel Jo?o Batista Castello;Baracat, Edmund Chada;Soares-Jr, José Maria;
Revista da Associa??o Médica Brasileira , 2010, DOI: 10.1590/S0104-42302010000600024
Abstract: apoptosis or programmed death is a biological phenomenon, which is essential for the development and maintenance of a cell population. in this process, senescent or damaged cells are eliminated after activation of a cell death program involving participation of pro-apoptotic molecules (fas, fas-l, bax, caspases 2, 3, 6, 7, 8 and 9). molecule activation causes typical morphological changes, such as cell shrinkage, loss of adhesion to the extracellular matrix and neighboring cells, chromatin condensation, dna fragmentation and formation of apoptotic bodies. anti-apoptotic molecules (bcl-2, flip) block the emergence and evolution of these cell changes and prevent cell death. the balance between molecules pro and anti-apoptotic ensures tissue homeostasis. when apoptosis is out of control, it contributes to the emergence of several neoplastic, autoimmune and neurodegenerative diseases. several inducing and inhibitors of apoptosis agents are recognized as potential weapons in the fight against diseases related to proliferation and cell death disorders among which stand out hormones. melatonin has been reported as important anti-apoptotic agent in various tissues by reducing cell calcium uptake, modulating expression of anti-oxidants and decreasing pro-apoptotic protein, such as bax. the knowledge of new agents capable to act on the course pf apoptosis is important and of great value for developing further therapies against many diseases. thus, the objective of this review was to elucidate the main aspects of cell death by apoptosis and the role of melatonin in this process.
Rat pinealectomy: a modified direct visual approach
Maganhin, Carla Cristina;Sim?es, Ricardo Santos;Fuchs, Luiz Fernando Portugal;Oliveira-Filho, Ricardo Martins;Sim?es, Manuel de Jesus;Evêncio Neto, Joaquim;Baracat, Edmund Chada;Soares Júnior, José Maria;
Acta Cirurgica Brasileira , 2009, DOI: 10.1590/S0102-86502009000400013
Abstract: purpose: to report a new, direct visual approach for rat pinealectomy. methods: eighty adult female rats (rattus norvegicus albinus epm-1 strain) were weighted and anesthetized intraperitoneally with 15 mg/kg xylazine and 30 mg/kg ketamine. the animal was fastened to a dissection table, an incision was made in the skin and the subcutaneous tissue, bringing the lambda into view. the skullcap was opened with a dental drill, bringing the cerebral hemispheres and the superior sagittal sinus into view. the pineal gland, located under the venous sinus, was removed in a single piece using tweezers. next, the bone fragment was returned to its place and the surgical layers were sutured. results: this new technique is easy to be done, avoids bleedings and removes only the pineal gland without damage to the remaining encephalon. in addition it makes possible the achievement of a sham surgery, allowing the pineal gland to remain intact. conclusion: the proposed technique intends to facilitate studies aiming to better understanding the complexity and importance of the pineal gland on reproductive and other body systems.
Parametros da urofluxometria e do estudo fluxo/press?o em pacientes uroginecológicas
Oliveira, Emerson de;Bezerra, Leonardo Robson Pinheiro Sobreira;Araújo, Maíta Poli de;Takano, Cláudia Cristina;Castro, Rodrigo de Aquino;Sartori, Marair Gracio Ferreira;Baracat, Edmund Chada;Gir?o, Manoel Jo?o Batista Castello;
Revista da Associa??o Médica Brasileira , 2008, DOI: 10.1590/S0104-42302008000200016
Abstract: objective: the purpose was to evaluate urine flow rates in an urodynamic study (us) of patients with symptoms of lower urinary tract dysfunction and to record effects of final urodynamic diagnosis and age on these variables. methods: retrospective analysis of 205 women who had undergone urogynecological investigation. patients were divided into three age groups: a (< 45 years); b (45 - 60 years) and c (> 60 years). comportment of urine flow rates and opening detrusor pressure with increasing age were studied and resulting charts recorded. comparison of maximum and average urine flow rates, opening detrusor pressure and detrusor pressure at the maximal flow according to final urodynamic diagnostic were also made. results: there was a decline of urine flow rates with increase of age, which was more significant between groups a x c and b x c. there was also a decline in opening detrusor pressure with aging, which was significant between groups a x c. when analyzing opening detrusor pressure according to final urodynamic diagnosis, a significant decline of this variable was observed in patients with urinary stress incontinence and intrinsic sphincter deficiency in relation to those who were asymptomatic. conclusion: urine flow rates decreased with age. women with urinary stress incontinence and intrinsic sphincter deficiency void at a lower detrusor pressure.
O cuidado psicológico ao médico em reprodu??o assistida: um enquadre diferenciado
Miranda, Keith Laura;Serafini, Paulo Cesar;Baracat, Edmund Chada;
Estudos de Psicologia (Campinas) , 2012, DOI: 10.1590/S0103-166X2012000100008
Abstract: health professionals have to deal with human suffering, even if they don't deal directly with interventions in the psychology area. this study was based on psychoanalysis, with the aim of understanding the aspects of human suffering which emerge with doctors in the exercise of their work with assisted human reproduction, considering the collective model concerning the difficult situations that they have to deal with in their clinics. twenty-two doctors working in hospitals and clinics in the state of s?o paulo, in both private and public facilities, volunteered to participate, ignoring sex, age and time since graduation. the drawing and storytelling thematic was used as the dialogical procedure. the interview was characterized as a special situation with the expression and communication of emotions generated in sessions related to the proposed theme. it was found that there was a need for the proposition of facilitating spaces for emotional expression, promoting the containment of human suffering and the occurrence of the transforming life reflection experiences of reality.
Novos conhecimentos sobre a flora bacteriana vaginal
Linhares, Iara Moreno;Giraldo, Paulo Cesar;Baracat, Edmund Chada;
Revista da Associa??o Médica Brasileira , 2010, DOI: 10.1590/S0104-42302010000300026
Abstract: the aim of this review is to update knowledge about the vaginal ecosystem, non-cultivation methods for bacterial identification (gene amplification), the lactobacillus species that comprise normal vaginal flora and influence of host genetics on bacterial interactions with local innate and acquired immune defenses. a medline (pubmed) search from 1997-2009 for relevant articles was performed and the most informative articles were selected. non-culture techniques (gene amplification) allow a comprehensive analysis of the vaginal ecosystem's composition. in the majority of women in the reproductive age there is a predominance of one or more species of lactobacillus: l. crispatus, l. inners and l gasseri. however, in other apparently healthy women there is a deficiency or complete absence of lactobacilli. instead, there is a substitution by other lactic acid-producing bacteria: atobium, megasphaera and/or leptotrichia species. the infectivity and/or proliferation of pathogenic bacteria in the vagina is suppressed by lactic acid production, by products of endogenous bacteria and by activation of local innate and acquired immunity. vaginal epithelial cells produce several compounds with anti-microbial activity. these cells have toll-like receptors on their membrane that recognize molecular patterns associated with pathogens. recognition leads to production of pro-inflammatory cytokines and stimulation of antigen-specific immunity. the production of igg and iga antibodies is also triggered in the endocervix and vagina in response to infection. vaginal flora composition and the immune mechanisms constitute important defenses. criteria of normal and abnormal flora have to be reviewed and genetic polymorphism can explain variations in flora composition. this new knowledge should be included in the clinical practice of gynecologists and obstetricians to improve patients care.
Evaluation of single nucleotide polymorphisms in microRNAs (hsa-miR-196a2 rs11614913 C/T) from Brazilian women with breast cancer
Linhares José Juvenal,Azevedo Marcos,Siufi Adalberto Abra?o,de Carvalho Cristina Valleta
BMC Medical Genetics , 2012, DOI: 10.1186/1471-2350-13-119
Abstract: Background Emerging evidence has shown that miRNAs are involved in human carcinogenesis as tumor suppressors or oncogenes. Single nucleotide polymorphisms (SNPs) located in pre-miRNAs may affect the processing and therefore, influence the expression of mature miRNAs. Previous studies generated conflicting results when reporting association between the hsa-miR-196a2 rs11614913 common polymorphism and breast cancer. Methods This study evaluated the hsa-miR-196a2 rs11614913 SNP in 388 breast cancer cases and 388 controls in Brazilian women. Polymorphism was determined by real-time PCR; control and experimental groups were compared through statistical analysis using the X2 or Fisher’s exact tests. Results The analysis of the SNPs frequencies showed a significant difference between the groups (BC and CT) in regards to genotype distribution (χ2: p = 0.024); the homozygous variant (CC) was more frequent in the CT than in the BC group (p = 0.009). The presence of the hsa-miR-196a2 rs11614913 C/T polymorphism was not associated with histological grades (p = 0.522), axillary lymph node positive status (p = 0.805), or clinical stage (p = 0.670) among the breast cancer patients. Conclusions The results of this study indicated that the CC polymorphic genotype is associated with a decreased risk of BC and the presence of the T allele was significantly associated with an increased risk of BC.
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