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Search Results: 1 - 10 of 109457 matches for " CHEN Zhao-an "
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Electron transport pathways of hydrogen photobiological production metabolism in CCCP-treated Tetraselmis subcordiformis

JI Chao-Fan,YU Xing-Ju,XUE Song,CHEN Zhao-An,ZHANG Wei,

微生物学通报 , 2011,
Abstract: The CCCP-treated marine microalga Tetraselmis subcordiformis could photo-produce hydrogen for sevaral days. Effect of photosynthetic inhibitors on hydrogen production helped to draw the network of hydrogen metabolism by CCCP-treated T. subcordiformis: at the beginning of hydrogen production, electrons provided to hydrogenase are mainly from photolysis and photofermentation via PS I and electron chain; thereafter, fermentative hydrogen production is considered as the most responsible for providing electrons to hydrogenase, which is independent of photosystem electron transfer chain. Fermentative metabolites assay indicated that light and DBMIB inhibited fermentative ethanol produc-tion that is the competitive pathway for the cellular reducing power, resulting in higher photo-hydrogen yield.
Subcellular Localization and Identification of Hydrogenase Isolated from the Marine Green Alga Platymonas subcordiformis Using Immunoprecipitation and MALDI-TOF MS

GUO Zhen,CHEN Zhao-An,YU Xing-Ju,JIN Mei-Fang,LI Wei,ZHANG Wei,

生物工程学报 , 2007,
Abstract: A marine unicellular green alga,Platymonas subcordiformis,was demonstrated to photobiologically produce hydrogen gas from seawater.The objective of this study was to localize and identify the hydrogenase isolated from P.subcordifo rmis.Adaptation in the presence of inhibitors of protein biosynthesis indicat ed that the hydrogenase was much more inhibited by cycloheximide than that by ch loramphenicol.The result suggested that the hydrogenase isolated from P.subc ordiformis is probably synthesized in cytoplasmic ribosomes.Both Western blot analysis and immunogold electron microscopy demonstrate that the P.subcordif ormis hydrogenase is mainly located in the chloroplast stroma.The proteins th at reacted specifically with the antibodies against the iron hydrogenase isolate d from Chlamydomonas reinhardtii were concentrated by immunoprecipitation.T he separated protein bands were cut out of the SDS-PAGE gel,in-gel digested b y trypsin,and analyzed by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry(MALDI-TOF MS).Mascot was employed for analysis of the MALDI data using the public databases NCBInr.The hydrogenase isolated from P.subcordiformis was identified to be the Fe-hydrogenase.
Optimization of Culture Medium and Photosynthetic Characteristics of Platymonas subcordiformis

LIU Yuan,CHEN Zhao-an,LU Hong-bin,LIU Chang-fa,JIN Mei-fang,GUO Zhen,ZHANG Wei,

过程工程学报 , 2007,
Abstract: 以均匀实验设计方法优化亚心形扁藻(Platymonas subcordiformis)培养基,采用U10*(108)均匀设计实验考察NO3--N, PO43--P, 缓冲体系(Tris-HAc)三因素对亚心形扁藻生长的影响,同时也考察了与其光合作用相关的PSII光化学活性、藻液pH、集光色素3个指标的变化. 结果表明,在NO3--N 9.5 mmol/L, PO43--P 0.34 mmol/L, Tris-HAc 6.7 mmol/L的优化条件下,亚心形扁藻生长迅速,15 d藻细胞密度增殖到(8.28±0.51)×106 mL-1. Tris-HAc体系能够维系藻细胞在最优pH条件下增殖,高浓度氮源利于PSII光化学活性保持恒定和集光色素的积累,从而提高了光能利用效率,促进藻细胞生长.
Relationships between Accumulation and Dying of Cyanobacteria and Black Spot

SHEN Ai-chun,XU Zhao-an,WU Dong-hao,

水生态学杂志 , 2012,
Abstract: Though water environmental of Taihu Lake was improved through many years integrated management,most of the area was eutrophicated.Under proper condition,black spot might be induced because large area algal bloom was still possible.Based on in situ enclose,impacts of large amount of blue-green algae accumulation on water environment was analyzed as well as its relationship with black spot forming.The results showed that,anoxia or hypoxia would be induced and the mean concentration of DO was 0.2 mg /L during the later period of the experiment.TN,NH 4+-N,and TP concentrations increased rapidly in short time when large mounts of blue-green algae dead,with the peak concentration of 16,48 and 46 times higher than the background values.Algal salvage was an effective measure to avoid water environment degrading.The caused black matters could also be induced because of algal death.Abundant polluted sediment was key factor in forming black spot,while wind-wave disturbance was necessary in order to induce typical black spot.
Improved algorithm of quasi-congruence in discrete-event system

ZHANG Ren-yuan,GAN Yong-mei,CHAO Wu-jie,WANG Zhao-an,

控制理论与应用 , 2012,
Abstract: We investigate the algorithm for reducing the time-complexity in computing the optimal quasi-congruence of discrete-event system (DES). Based on the algorithms provided by Paige, Tarjan and Ferandez, the algorithm related to the relational coarsest partition problem is adopted to figure out the optimal quasi-congruence with time complexity O(mlogn). This algorithm is applicable when the DES is complicated, especially when the number of observable events is small. Compared with the algorithm with time complexity O(mn) proposed by Ramadge and Wonham, this algorithm is less time-consuming. In addition, the improvement of boundary conditions in computing the optimal quasi-congruence is discussed. The effectiveness of the proposed algorithm is validated by simulation results.
Study on Context Awareness Application Framework Based on State Machine

YU Zhao-An,ZHANG Shen-Sheng,ZHANG-Yong,TONG Hong-Xia,

计算机科学 , 2006,
Abstract: Context awareness is one of important research areas in the pervasive computing research. Many researchers have proposed some context awareness application frameworks in order to facilitate the development of context awareness applications. However, many context awareness application frameworks tend to let application subscribe or query related context information directly. Developers should try to pay attention to many kinds of context information and relationship among them and define many similar rules to identify different actions. Aiming at solving these problems, a concept of environmental state in the information space is introduced and a kind of improved context awareness application framework based on state machine is presented on the base of frameworks research. Also, some components are designed to support context awareness application development based on state machine.
Optimal Generator Portfolio in Day-Ahead Market under Uncertain Carbon Tax Policy  [PDF]
Shengyuan Chen, Ming Zhao
American Journal of Operations Research (AJOR) , 2011, DOI: 10.4236/ajor.2011.14031
Abstract: The global liberalization of energy market and the evolving carbon policy have profound implication on a producer’s optimal generator portfolio problem. On one hand, the daily operational flexibility from a well- composed generator portfolio enables the producer to implement a more aggressive bidding strategy in the liberalized day-ahead market on a daily basis; on the other hand, the evolving carbon policy demands the long term robustness of a generator portfolio: it should be able to generate stable cash flow under different stages of the evolving carbon tax policy. It is computationally very challenging to incorporate the daily bidding strategy into such a long term generator portfolio study. We overcome the difficulty by a powerful vertical decomposition. The long term uncertainty of carbon tax policy is simulated by scenarios; while the daily electricity price fluctuation with jumps is modeled by a more complicated Markov Regime Switching model. The proposed model provides the senior executives an efficient quantitative tool to select an optimal generator portfolio in the deregulated market under evolving carbon tax policy.
Psychosocial Factors for Women Requesting Cesarean Section  [PDF]
Yajuan Zhao, Shuangyun Chen
International Journal of Clinical Medicine (IJCM) , 2013, DOI: 10.4236/ijcm.2013.49071
Abstract: Background: Rates of caesarean section are progressively increasing in many parts of the world. As a result of psychosocial factors, there has been an increasing tendency for pregnant women without justifiable medical indications for caesarean section to ask for this procedure in China. The psychosocial factors for requesting cesarean section were analyzed in our study. Methods: A self-made questionnaire and the State-Trait Anxiety Inventory (STAI) and Self-rating Depression Scale (SDS) were administered to evaluate lying-in women’s psychosocial state. Results: The proportion of lying-on women’s age and education degree was different significantly between the two groups (p < 0.01). In the study groups, the proportion of lying-on women who were in lower economic degree and in her first para was markerly higher than control (88.62% vs. 63.24%; 13.32vs. 3.42%, p < 0.01). In the study groups, the proportion of women who felt fearful, anxious, depressed and lacking confidence was higher than control. The ratio of women with university education in the CS group was higher than vaginal delivery group. The proportion of lying-on women without correct delivery knowledge was higher than control, but the difference was not significant (p > 0.05). By logistic regression, we found that for primipara, higher education degree, anxiety and lacking confidence were the dangerous factors for cesarean, while lower economic degree was a defendant factor. Conclusion: There were five main psychosocial factors such as education degree and economic state, parity, anxiety and confidence of lying-on women affecting the choice of the delivery way. The nulliparous women who feel anxious without confidence in nice economic state, with better education have higher risk to choose cesarean.
The Spread and Reception of Don Quixote in China  [PDF]
Guoen Chen, Hongying Zhao
Advances in Literary Study (ALS) , 2014, DOI: 10.4236/als.2014.22011

The reception of Don Quixote in China started in the early 20th century, with its media Japanese literature and Russian literature and its initiating point Chinese social reforms as well as intellectuals’ self-reflections on their role. Zhou Zuoren was the very first to make a scientific criticism of the novel Don Quixote while Lu Xun took the lead in exploring the essence of Don Quixote’s spirit, defining it as mixing realism with idealism, which would have a negative impact on its readers. Lu Xun was thus inspired to create a character named AQ, eternally charming readers. Feiming, as an explorer of the truth of humanity, orientalized Don Quixote’s spirit in the form of parody. Don Quixote has become a universal symbol of the human spirit, has aroused the attention of Chinese intellectuals, and has even directly “engaged in” the controversies in the Chinese literary world. By the end of 20th century, translations and research on Don Quixote attained new achievements, Don Quixote again winning recognition as an essential theoretical category in helping researchers in the literary world elaborate on the phenomenon of human spirit.

The Network Educational Resources Construction on Meteorology Based on Data Mining  [PDF]
Rui Chen, Yang Zhao
Open Journal of Social Sciences (JSS) , 2015, DOI: 10.4236/jss.2015.36001

With the vigorous development of the modern network education technology, the construction of network resource developed towards digital, intelligent and personalized, in order to satisfy the distance learners of different learning needs. By analyzing the problems currently learners in the use of the repository for learning generated by the proposed use of data mining techniques to further planning and construction of the repository, and on how to improve the organizational structure of the repository, and improve the utilization of the repository, personalized to meet the current in-depth research and analysis of learning problems.

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