oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 160 )

2018 ( 1521 )

2017 ( 1362 )

2016 ( 1407 )

Custom range...

Search Results: 1 - 10 of 111665 matches for " CHEN Yong-Sheng "
All listed articles are free for downloading (OA Articles)
Page 1 /111665
Display every page Item
Cellular responding kinetics based on a model of gene regulatory networks under radiotherapy  [PDF]
Jin-Peng Qi, Yong-Sheng Ding, Shi-Huang Shao, Xian-Hui Zeng, Kuo-Chen Chou
Health (Health) , 2010, DOI: 10.4236/health.2010.22021
Abstract: Radiotherapy can cause DNA damage into cells, triggering the cell cycle arrest and cell apop-tosis through complicated interactions among vital genes and their signal pathways. In order to in-depth study the complicated cellular res- ponses under such a circumstance, a novel mo- del for P53 stress response networks is pro- posed. It can be successfully used to simulate the dynamic processes of DNA damage trans-ferring, ATM and ARF activation, regulations of P53-MDM2 feedback loop, as well as the toxins degradation. Particularly, it has become feasible to predict the outcomes of cellular response in fighting against genome stresses. Consequently, the new model has provided a reasonable framework for analyzing the complicated regu-lations of P53 stress response networks, as well as investigating the mechanisms of the cellular self-defense under radiotherapy.
Immunity of Foot-and-Mouth Disease Serotype Asia 1 by Sublingual Vaccination
Hao-tai Chen, Yong-sheng Liu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0063839
Abstract: Foot-and-mouth disease virus (FMDV) causes vesicular disease of cloven-hoofed animals, with severe agricultural and economic losses. Here we present study using a sublingual (SL) route with the killed serotype Asia 1 FMDV vaccine. Guinea pigs were vaccinated using a commercially available vaccine formulation at the manufacturer’s recommended full, 1/4, and 1/16 antigen doses. Animals were challenged with homologous FMDV Asia1 strain at various times following vaccination. All control guinea pigs exhibited clinical disease, including fever, viremia, and lesions, specifically vesicle formation in feet. Animals vaccinated with the 1/16 and 1/4 doses were protected after challenge at days 7, 28, and 35 post vaccination. These data suggest that effective protection against foot-and-mouth disease can be achieved with 1/16 of the recommended vaccine dose using SL vaccination, indicating that the sublingual route is an attractive alternative for the administration of the FMDV vaccine.
Batch-normalized Maxout Network in Network
Jia-Ren Chang,Yong-Sheng Chen
Computer Science , 2015,
Abstract: This paper reports a novel deep architecture referred to as Maxout network In Network (MIN), which can enhance model discriminability and facilitate the process of information abstraction within the receptive field. The proposed network adopts the framework of the recently developed Network In Network structure, which slides a universal approximator, multilayer perceptron (MLP) with rectifier units, to exact features. Instead of MLP, we employ maxout MLP to learn a variety of piecewise linear activation functions and to mediate the problem of vanishing gradients that can occur when using rectifier units. Moreover, batch normalization is applied to reduce the saturation of maxout units by pre-conditioning the model and dropout is applied to prevent overfitting. Finally, average pooling is used in all pooling layers to regularize maxout MLP in order to facilitate information abstraction in every receptive field while tolerating the change of object position. Because average pooling preserves all features in the local patch, the proposed MIN model can enforce the suppression of irrelevant information during training. Our experiments demonstrated the state-of-the-art classification performance when the MIN model was applied to MNIST, CIFAR-10, and CIFAR-100 datasets and comparable performance for SVHN dataset.
Active volatiles of cabernet sauvignon wine from Changli County  [PDF]
Yong-Sheng Tao, Hua Li
Health (Health) , 2009, DOI: 10.4236/health.2009.13029
Abstract: This study investigated the contribution of vola-tile compounds to the overall aroma of Cabernet Sauvignon wines from Changli County (China). Wine samples were collected from vintages from 2000 to 2005. Volatile compounds were ex-tracted by PDMS solid-phase micro-extraction fi- bers and identified by Gas Chromatography-Ma- ss Spectrometry (GC-MS). A total of 65 volatile compounds were identified and quantified, in-cluding higher alcohols, ethyl and acetate esters, and fatty acids. According to their odor active values (OA-Vs), 21 volatile compounds were con- sidered to be the powerful impact odorants of Cabernet Sauvignon wines from Changli. Odor descriptions of impact volatiles suggested Cab-ernet Sauvignon red wines from Changli County as having a complex aroma, which included not only pleasant floral and fruity odors, but also cheese, clove flavors, and grassy and smoky aromas.
Software model checking based on hierarchical unit partition
基于层次单元划分的软件模型检测技术

CHEN Chen,CHEN Yong-sheng,
陈晨
,陈永生

计算机应用 , 2008,
Abstract: This paper reviewed some prevalent trends in this domain in recent years, then proposed a software model checking scenario, which was based on hierarchical unit partition and heuristic search. It has three phases, which are preprocess, unit partition and state space search. There is on-the-fly method in this scenario to improve the performance of model checking. Experiments prove that this model checking scenario works well on solving state explosion problem.
The expression, purification and activity detect of quinolinic acid phosphoribosyl transferase and nicotinic acid phosphoribosyl transferase from Escherichia coli
大肠杆菌来源的喹啉酸磷酸核糖转移酶和烟酸磷酸核糖转移酶的表达纯化及酶活性的初步检测

CHEN Yong-Sheng,ZHOU Qiang,ZHAO Sheng-Yin,
陈永升
,周强,赵圣印

微生物学通报 , 2012,
Abstract: Objective] The expression, purification of quinolinic acid phosphoribosyl transferase (QPRT) and nicotinic acid phosphoribosyl transferase (NaPPT) from Escherichia coli (E. coli) were undertaken in prokaryotic expression system and activity detection of QPRT and NaPPT in vitro. Methods] The NadC encoding QPRT and PncB encoding NaPPT were cloned and then ligated into pET28a and pRSETB to generate the expression plasmid pET28a-NadC and pRSETB-PncB, respectively. The plasmids were expressed in E. coli BL21(DE3) and enzymes were purified in vitro. Enzymes activity were detected by analyzing the reaction mixture on a high performance liquid chromatography (HPLC) system. Results] QPRT and NaPPT were expressed and purified, the results indicate that quinolinic acid can be transformed into nicotinic acid through regioselective decarboxylation catalyzed by recombinant QPRT and NaPPT sequentially.
Enhancement of CTLs induced by DCs loaded with ubiquitinated hepatitis B virus core antigen
Jian-Hua Chen,Yong-Sheng Yu,Xiao-Hua Chen,Hong-Hong Liu
World Journal of Gastroenterology , 2012, DOI: 10.3748/wjg.v18.i12.1319
Abstract: AIM: To investigate whether hepatitis B virus (HBV) could induce a hepatitis B virus core antigen (HBcAg)-specific cytotoxic T lymphocyte (CTL) response in vitro by dendritic cells (DCs) transduced with lentiviral vector-encoding ubiquitinated hepatitis B virus core antigen (LV-Ub-HBcAg). METHODS: Recombinant LV-Ub-HBcAg were transfected into highly susceptible 293 T cells to obtain high virus titres. Bone marrow-derived DCs isolated from BALB/c mice were cultured with recombinant granulocyte-macrophage colony-stimulating factor and recombinant interleukin (IL)-4. LV-Ub-HBcAg, lentiviral vector-encoding hepatitis B virus core antigen (LV-HBcAg), lentiviral vector (LV) or lipopolysaccharide were added to induce DC maturation, and the DC phenotypes were analyzed by flow cytometry. The level of IL-12 in the supernatant was detected by enzyme-linked immunosorbent assay (ELISA). T lymphocytes were proliferated using Cell Counting Kit-8. DCs were cultured and induced to mature using different LVs, and co-cultured with allogeneic T cells to detect the secretion levels of IL-2, IL-4, IL-10 and interferon-γ in the supernatants of T cells by ELISA. Intracellular cytokines of proliferative T cells were analyzed by flow cytometry, and specific CTL activity was measured by a lactate dehydrogenase release assay. RESULTS: LV-Ub-HBcAg-induced DCs secreted more IL-12 and upregulated the expression of CD80, CD86 and major histocompatibility class II. DCs sensitised by different LVs effectively promoted cytokine secretion; the levels of IL-2 and interferon-γ induced by LV-Ub-HBcAg were higher than those induced by LV-HBcAg. Compared with LV-HBcAg-transduced DCs, LV-Ub-HBcAg-transduced DCs more efficiently stimulated the proliferation of T lymphocytes and generated HBcAg-specific cytotoxic T lymphocytes. CONCLUSION: LV-Ub-HBcAg effectively induced DC maturation. The mature DCs efficiently induced T cell polarisation to Th1 and generated HBcAg-specific CTLs.
Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification
Hao-tai Chen, Jie Zhang, Yong-sheng Liu, Xiang-tao Liu
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-489
Abstract: Foot-and-mouth disease virus (FMDV) is a member of the genus Aphthovirus of the family Picornaviridae, which is divided into seven serotypes with no cross-protection conferred among the serotypes [1]. FMDV serotypes O, A, C are widely distributed worldwide, whereas FMDV serotypes SAT 1, SAT 2, SAT 3 are normally restricted to Africa and FMDV serotype Asia 1 to Asia [2,3]. Due to the aggressive nature of foot-and-mouth disease (FMD), outbreaks usually result in severe economic losses and impact on both national and international trade within the livestock and animal products [4-6]. Rapid and accurate diagnosis of any suspected FMD cases is of utmost urgency to control this veterinary infection given the extreme contagiousness of the causative virus.Conventional laboratory diagnosis of FMD was made by enzyme-linked immunosorbent assay (ELISA) detection of specific viral antigens and by observation of cytopathogenic effects in cell culture [4,7-9]. Alternatively, conventional reverse transcriptase polymerase chain reaction (RT-PCR) [5,10-14] and real-time RT-PCR [6,15-18] were developed to complement primary diagnostic techniques for the FMDV infection. These assays were time-consuming and laborious, which required centralized laboratory facilities and clinical specimen submissions, resulted in the delay of FMDV diagnosis. Given these problems, a rapid, simple, and practical assay to detect FMDV in animal and its products was therefore required in clinical practice.A novel nucleic acid amplification method, termed reverse transcription loop-mediated isothermal amplification (RT-LAMP), which was applied successfully to the detection of influenza A virus, Newcastle disease virus and classical swine fever virus, porcine reproductive and respiratory syndrome virus [19-22]. In this study, RT-LAMP assay was developed a diagnostic method for the typing of FMDV serotype Asia 1, and then the sensitivity and specificity of the RT-LAMP assay were evaluated using the clincal sampl
Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification
Hao-tai Chen, Jie Zhang, Yong-sheng Liu, Xiang-tao Liu
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-510
Abstract: Foot-and-mouth disease virus (FMDV) is a member of the genus Aphthovirus of the family Picornaviridae, which is divided into seven serotypes with no cross-protection conferred among the serotypes [1]. Due to the aggressive nature of foot-and-mouth disease (FMD), outbreaks usually result in severe economic losses and impact on both national and international trade within the livestock and animal products [2]. Rapid and accurate diagnosis of any suspected FMD cases is of utmost urgency to control this veterinary infection given the extreme contagiousness of the causative virus.Conventional laboratory diagnosis of FMD was made by ELISA detection of specific viral antigens and by observation of cytopathic effects in cell culture [3]. Alternatively, the conventional reverse transcriptase polymerase chain reaction (RT-PCR) [4] and real-time RT-PCR [2,5] were developed to complement primary diagnostic techniques for the FMDV infection. These assays were time-consuming and laborious, which required centralized laboratory facilities and clinical specimen submissions, resulted in the delay of FMDV diagnosis. Given these problems, a rapid, simple and practical assay to detect FMDV in animal and its products was therefore required in clinical practice.A reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied successfully to the detection of many animal viruses [6-9]. In this study, we evaluated the potential of RT-LAMP for the development of a simple and rapid detection system for FMDV RNA.The strains O/CHA/1999, A/CHA/2009, C/SU/1958, Asia 1/CHA/2005 were developed RT-LAMP method and then the stain O/CHA/1999 was to test the detection limit. The serotypes O and Asia 1 were propagated in IBRS-2 cells, and the serotypes A and C were inoculated in 3-day suckling mice, respectively.Other field isolates including classical swine fever virus (CSFV), swine vesicular disease virus (SVDV), porcine reproductive and respiratory syndrome virus (PRRSV) and Japane
H.264/SVC parameter optimization based on quantization parameter, mgs fragmentation, and user bandwidth distribution
Xu CHEN, Ji-hong ZHANG, Wei LIU, Yong-sheng LIANG and Ji-qiang FENG
EURASIP Journal on Advances in Signal Processing , 2013, DOI: 10.1186/1687-6180-2013-10
Abstract: In the situation of limited bandwidth, how to improve the performance of scalable video coding plays an important role in video coding. The previously proposed scalable video coding optimization schemes concentrate on reducing coding computation or trying to achieve consistent video quality; however, the connections between coding scheme, transmission environments, and users' accesses manner were not jointly considered. This article proposes a H.264/SVC (scalable video codec) parameter optimization scheme, which attempt to make full use of limited bandwidth, to achieve better peak signal-to-noise ratio, based on the joint measure of user bandwidth range and probability density distribution. This algorithm constructs a relationship map which consists of the bandwidth range of multiple users and the quantified quality increments measure, QPe, in order to make effective use of the video coding bit-stream. A medium grain scalability fragmentation optimization algorithm is also presented with respect to user bandwidth probability density distribution, encoding bit rate, and scalability. Experiments on a public dataset show that this method provides significant average quality improvement for streaming video applications.
Page 1 /111665
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.