oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 4 )

2019 ( 229 )

2018 ( 1796 )

2017 ( 1680 )

Custom range...

Search Results: 1 - 10 of 121439 matches for " CHEN XIAO QING "
All listed articles are free for downloading (OA Articles)
Page 1 /121439
Display every page Item
Isolation and Characterization of Pepsin-Solubilized Collagen from the Skin of Black Carp (Mylopharyngdon piceus)  [PDF]
Guo Ping Wu, Xiao Ming Wang, Li Ping Lin, Su Hua Chen, Qing Qing Wu
Advances in Bioscience and Biotechnology (ABB) , 2014, DOI: 10.4236/abb.2014.57076
Abstract:

To make more effective use of underutilized resources, pepsin-solubilized collagen (PSC) was successfully extracted from the skin of black carp (Mylopharyngdon piceus) with a yield of 45.7% based on dry weight. The PSC comprising two identical α1-chains and one α2-chain with no disulfide bond was characterized as type I, and it contained 195 imino acid residues vs. 1000 amino acid residues. The collagen showed an absorption edge around 218 nm, which was lower than the maximum absorption wavelength of other PSC. The denaturation temperature of PSC was 25.6°C, which was lower than that of porcine collagen by approximately 11°C. The isoelectric point (pI) was estimated to be 8.23, and the collagen was soluble at an acidic pH as well as below 40 g/L NaCl. It is thought that the high yield and stability of PSC from the skin of black carp warrant its application as a new source of collagen for industrial purposes.

Extraosseous Ewing's sarcoma arising from the pterygomandibular space  [PDF]
Wei Li, Jian Tao Huang, Xiao Qing Chen, Rong Hua Shi, Lei Jiang, Yun Fu Zhao
Open Journal of Stomatology (OJST) , 2011, DOI: 10.4236/ojst.2011.13018
Abstract: Extraosseous Ewing’s sarcoma (EES) arising in head and neck is extremely rare. We report on a rare case of EES originating from the pterygomandibular space. A 15-year-old boy was seen with a rapidly growing mass in the right upper neck, difficulty in opening mouth, dysphagia, numbness in the right lower lip and buccal skin. Clinical and radiological examinations indicated that a soft tissue mass origi-nated from the pterygomandibular space with the submandibular space and mouth floor extension. Histological and immunohistochemical evaluations of the biopsy specimen revealed poorly differentiated small blue round cells with positive stains for CD99 and neuron-specific enolase. According to the clinical manifestation, CT and MRI findings, histological pattern and the results of the immunohistochemical studies, the final diagnosis was EES. Our patient was treated with chemotherapy and radiotherapy. The lesion recurred locally after 10 months and he died of multiple distant metastases 22 months later. Early and confident diagnosis coupled with combined surgical excision and modern chemotherapy/radiotherapy appears to be the most effective treatment plan.
Comment on "Vortex Liquid Crystal in Anisotropic Type II Superconductors"
Xiao Hu,Qing-Hu Chen
Physics , 2003, DOI: 10.1103/PhysRevLett.92.209701
Abstract: This is a Comment on "Vortex Liquid Crystal in Anisotropic Type II Superconductors" by E. W. Carlson et al. in PRL, vol.90, 087001 (2003) [cond-mat/0209175].
Nonequilibrium Phase Transitions of Vortex Matter in Three-Dimensional Layered Superconductors
Qing-Hu Chen,Xiao Hu
Physics , 2002, DOI: 10.1103/PhysRevLett.90.117005
Abstract: Large-scale simulations on three-dimensional (3D) frustrated anisotropic XY model have been performed to study the nonequilibrium phase transitions of vortex matter in weak random pinning potential in layered superconductors. The first-order phase transition from the moving Bragg glass to the moving smectic is clarified, based on thermodynamic quantities. A washboard noise is observed in the moving Bragg glass in 3D simulations for the first time. It is found that the activation of the vortex loops play the dominant role in the dynamical melting at high drive.
RP-HPLC-DAD detrmination of flavonoids: separation of quercetin, luteolin and apigenin in Marchantia Convoluta
Xiao-qing Chen,Jian-bo Xiao
Iranian Journal of Pharmaceutical Research , 2005,
Abstract: High performance liquid chromatography coupled with photodiode array detector (HPLCDAD)has been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as Chinese herbal drugs and products from factories. This work was designed, therefore, to develop an HPLC-DAD system to separate quercetin, luteolin and apigenin and to quantify them in extractive solutions from Marchantia convoluta. Flavonoids were analyzed on a Kromasil RP-C18 column; using a mobile phase, consisted of methanolacetonitrile-acetic acid-phosphoric acid-H2O (200:100:10:10:200, V/V); under the following conditions: detecting wavelength, 352 nm; flow rate, 0.60 ml/min; the sensitivity, 0.05 AUFS and the volume of injecting sample, 6.0 μl. The HPLC system was operated at ambient temperature (28±1°C). The method showed linearity for quercetin, luteolin and apigenin in the range 2.0-20.8, 2.2-24.0 and 1.6-20.0 μg/ml respectively, and the R.S.D. of the slope of the three lines was, respectively, 0.33%, 1.21% and 2.49% for quercetin, luteolin and apigenin.The aqueous and ethanol 80% extractive solutions showed linear response 1.5-15 μl/ml and ethanol 50% extractive solution in range 1.0-10 μl/ml. Precision and accuracy were determined for ethanol 80% extractive solution, in concentration of 10 μl/ml. The recoveries were 95.92-98.10%, 92.18-95.13% and 98.72-103.19% for quercetin, luteolin and apigenin respectively.RSD of results was 2.83-3.62%. The HPLC method showed an excellent performance in separating the flavonoids quercetin, luteolin and apigenin in Marchantia convoluta extracts, since the presence of interference has been previously evaluated and the mobile phase waschose carefully.
Effect of erythropoietin on dynamic changes of TLR4,NF-κB,and IL-6 in rat model with necrotizing enterocolitis
Xiao-qing CHEN,Hua XU,Xiao-ping LUO
Medical Journal of Chinese People's Liberation Army , 2011,
Abstract: Objective To explore the protective effect and the mechanism of erythropoietin(EPO) on dynamic expressions of TLR-4,NF-κB,and IL-6 in the intestinal tissue of neonatal rat model with necrotizing enterocolitis(NEC).Methods A total of 90 SD rats(3 days old) were divided randomly into 3 groups(30 each) as follows: normal control group,NEC group,and EPO group.The NEC rat model was established by feeding the subjects with rat-milk substitutes after separation from the mother rats.The subjects were then exposed to hypoxia and cold stress.The EPO-treated rats were fed with the substitute of rat-milk containing EPO(0.1U/ml),and were exposed to hypoxia and cold stress as well.The rats in the control group were raised by their mothers and received no intervention.Ten rats from each group were sacrificed at 24,48,and 72h,respectively.The last 3cm of the terminal ileum was excised and the histological changes of the intestinal tissues were scored after H-E staining.Scores ≥2 were considered NEC.Fluorescence quantitative PCR was used to evaluate the dynamic levels of TLR4 mRNA and NF-κB mRNA.ELISA was used to determine the expression of IL-6.Results The TLR4 mRNA expression in the NEC group increased at 24h,peaked at 48h,and slightly decreased at 72h.The expressions in all time points increased significantly compared with those in the control group(P < 0.01).The rats in the EPO group expressed high levels of TLR4 mRNA at 24h.However,the expression rapidly declined later,and was significantly lower at 48 and 72h compared with those in the NEC group(P < 0.01).The expressions of NF-κB mRNA and IL-6 were significantly higher in the NEC group than in the control group at 48 and 72 h(P < 0.01).The expressions considerably increased in the EPO group at 48h,then declined at 72h,and were significantly lower than those in the NEC group(P < 0.01).No apparent pathological change was found in all groups at the time points of 24 and 48h.However,tissue lesion was observed in the NEC and EPO groups at 72 h,and the histological score was significantly higher in the NEC group(2.83±0.56) than in the EPO group(1.53±0.35,P < 0.01).Conclusions In the intestinal tract of the neonatal rat with NEC,innate immune response initiates earlier than the acquired immune response.Inflammatory response appears ahead of the tissue lesion,implying that the inflammatory response induces lesion.The enteral administration of human milk with a physiologic concentration of EPO may downregulate the TLR4 mRNA expression and inhibit the release of inflammatory mediators,thereby reducing intestinal tissue inju
Effect of erythropoietin on intestinal injury and bacterial translocation in neonatal rat model of necrotizing enterocolitis
Xiao-qing CHEN,Xiao-ping LUO
Medical Journal of Chinese People's Liberation Army , 2012,
Abstract: Objective  To observe the influence of erythropoietin (EPO) on intestinal histopathological changes and bacterial translocation (BT) in neonatal rat model of necrotizing enterocolitis (NEC), and explore the protective effect of EPO against NEC. Methods  Seventy-five three-day-old SD rat pups were randomly divided into three groups (25 in each group): normal control group, NEC model group and EPO intervention group. The rat pups in normal control group were placed together with their mothers and breast fed, receiving no other intervention. NEC model group rats were separated from their mothers, housed in an incubator, and gavaged with rat-milk substitute, then experienced hypoxia (breathing 100% nitrogen gas for 90s) and cold stress (4℃ for 10min) three times daily for 3 days. EPO intervention group rats were fed with the substitute of rat-milk supplemented with 0.1U/ml of EPO, and they were also given hypoxia and cold stress similar to that of the NEC model group. Blood samples were obtained via cardiac puncture, and 2-cm-length of terminal ileum proximal to the ileocecal valve were obtained from the animals on the 4th day. The histopathological changes in terminal ileum were scored after hematoxylin-eosin (HE) staining, and the scores ≥2 were defined as NEC. To determine the incidence of bacterial translocation, 16S rRNA real-time fluorescence quantitative PCR was used to detect the bacterial DNA in blood samples. Results  Compared with the NEC model group, the mean rank-sum rate of the intestinal histopathological score (39.4583 vs 53.8696), NEC incidence [25%(6/24) vs 57%(13/23)] and bacterial translocation rate [17% (4/24) vs 65%(15/23)] in EPO intervention group were significantly lowered (P < 0.05, P < 0.01). Conclusion  Enteral EPO administration is not only effective for reduction of the severity and incidence of NEC, but also for decrease of the bacterial translocation rate in neonatal rat models.
COMPARISON OF VOLATILE COMPONENTS FROM MARCHANTIA CONVOLUTA OBTAINED BY MICROWAVE EXTRACTION AND PHYTOSOL EXTRACTION
YAN,CHEN; XING YU,JIANG; XING,TONG; XIAO QING,CHEN;
Journal of the Chilean Chemical Society , 2008, DOI: 10.4067/S0717-97072008000200016
Abstract: the volatile oils from marchantía convoluta were obtained by microwave extraction (me) and phytosol extraction (pe) methods. the attained extracts were analyzed by gas chromatography with mass spectrometric detector (gc-ms). the compounds were identified according to their retention indices and mass spectra (el, 70 ev). a total of fourteen compounds were identified in me extract accounting for 80.72% of total peak area. isolongifolene (24.588%), 1, 2-benzenedicarboxylic acid, butyl cyclohexyl ester (10.768%), pyrene (9.328%) and hexadecanoic acid, ethyl ester (8.570%) were the major compounds identified from me extract. the analytical results of me extract were compared with pe method. the me extract was found to be markedly different from that of pe extract. a total of seven compounds in pe extract were identified accounting for 74.275% total peak area. 1-phenanthrenecarboxylic acid, 1,2,3,4,4a,9,10, 10a-octahydro-1, 4a-dimethyl-7-(1-methylethyl) methyl ester were the major compounds
COMPARISON OF VOLATILE COMPONENTS FROM MARCHANTIA CONVOLUTA OBTAINED BY MICROWAVE EXTRACTION AND PHYTOSOL EXTRACTION
CHEN YAN,JIANG XING YU,TONG XING,CHEN XIAO QING
Journal of the Chilean Chemical Society , 2008,
Abstract: The volatile oils from Marchantía convoluta were obtained by microwave extraction (ME) and phytosol extraction (PE) methods. The attained extracts were analyzed by gas chromatography with mass spectrometric detector (GC-MS). The compounds were identified according to their retention indices and mass spectra (El, 70 eV). A total of fourteen compounds were identified in ME extract accounting for 80.72% of total peak area. Isolongifolene (24.588%), 1, 2-benzenedicarboxylic acid, butyl cyclohexyl ester (10.768%), pyrene (9.328%) and hexadecanoic acid, ethyl ester (8.570%) were the major compounds identified from ME extract. The analytical results of ME extract were compared with PE method. The ME extract was found to be markedly different from that of PE extract. A total of seven compounds in PE extract were identified accounting for 74.275% total peak area. 1-Phenanthrenecarboxylic acid, 1,2,3,4,4a,9,10, 10a-octahydro-1, 4a-dimethyl-7-(1-methylethyl) methyl ester were the major compounds
(3S,NR)-3-Hydroxymethyl-2-methyl-2-(3-methylbut-2-en-1-yl)-1,2,3,4-tetrahydroisoquinolinium bromide–1,1′-bi-2-naphthol (1/1)
Xiao-Qing Wu,Qing-Chun Huang,Xiao-Zhen Chen,Guo-You Li
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536810007944
Abstract: In the title compound, C16H24NO+·Br ·C20H14O2, the N-heterocyclic six-membered ring assumes a half-chair conformation. The two naphthalene ring systems are nearly perpendicular to one another, making a dihedral angle of 89.5 (2)°. Intermolecular O—H...Br hydrogen bonding helps to stabilize the crystal structure.
Page 1 /121439
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.