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Search Results: 1 - 10 of 112277 matches for " CHEN Ming-Jie "
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The Application and Discussion of Network Technology in the "Change Open-hearth Furnace to Converter" Project of No.1 St eel-making Plant of WISGCO
网络计划技术在武钢一炼钢厂平炉改转炉工程中的应用与探讨

CHEN Ming-jie,TAN Jian-wu,
陈明杰
,谭建武

系统工程理论与实践 , 2002,
Abstract: The application of network iechnology in the "change open-hearth furnace to converter" project of WISGCO, not only undertaked the accomplish of the WISGCO policy aim ahead of schedule in 1998, but also achieved great economic and social efficiency.
A NEW PREDICATIVE NEURAL NETWORK METHOD FOR INSECT FORECAST
一种新的昆虫神经网络预测预报方法

WAN XIN-HUI,CHEN KAI-ZHOU,NIU MING-JIE,
文新辉

系统科学与数学 , 1995,
Abstract: The insect forecast is a difficult problem in plant protection. This paper has analysed the main factors which influence the insect density and made a new neural network predicative model. This method has a capacity for learning and can solve the forecasting problem which the traditional method could not. We have used this method to forecast the insect density in cotton field.The result shows that this method is better than other predicative methods and very useful in practice.
Expression of cold-induced gene Cor1 in Volvariella volvacea during low temperature treatment
草菇冷诱导基因Cor1在低温下表达变化的研究

QIAO Na,WANG Hong,CHEN Ming-Jie,
乔 娜
,汪 虹,陈明杰

菌物学报 , 2009,
Abstract: 将草菇菌株V23和耐低温诱变草菇菌株VH3的菌丝在4℃下处理不同时间,分别提取RNA,反转录获得cDNA作为模板,构建含Cor1基因和GPD内标基因片段的质粒作为标准品,采用荧光定量PCR技术研究Cor1基因在低温下的表达变化.结果表明草菇V23菌株的Cor1基因未经低温处理的表达量最大,低温处理后表达量持续下降,在6h达到最低值,8h开始该基因的表达量上升;而VH3菌株Cor1基因低温处理后表达量明显增加,2h达到最大值,4h下降,但6h开始上升,10h又下降.通过推测Cor1基因表达与草菇耐低温特性有相关性,为今后利用草菇自身的抗寒机制进行遗传改造提供理论依据.
Preparation and Characterization of Cu(In,Ga)Se2 Thin Films by Selenization of Cu0.8Ga0.2 and In2Se3 Precursor Films
Jiang Liu,Da-Ming Zhuang,Ming-Jie Cao,Chen-Yue Wang,Min Xie,Xiao-Long Li
International Journal of Photoenergy , 2012, DOI: 10.1155/2012/149210
Abstract: Se-containing precursor films with two different compositions were prepared by magnetron sputtering from and targets, and then were selenized using Se vapor. The effects of precursor composition and selenization temperature on the film properties were investigated. The results show that Se phase plays a critical role in film growth and electrical properties of CIGS films. The Cu-rich films exhibit different surface morphology and better crystallinity, as compared to the Cu-poor films. All the CIGS films exhibit p-type conductivity. The resistivity of the Cu-rich films is about three orders of magnitude lower than that of the Cu-poor films, which is attributed to the presence of p-type highly conductive Se phase. 1. Introduction Cu( )Se2 (CIGS) thin films have received considerable attention in recent years because of their application in solar cells, which have many advantages in high conversion efficiency [1, 2], the possibility of low-cost production [3, 4], and long-term stability [5, 6]. The world-record efficiency for small-area CIGS-based thin film solar cells has recently surpassed 20% [7]. One of the special qualities of the CIGS material is its variable band-gap [8]. The bandgap of chalcopyrite CIGS absorber could vary from 1.04?eV in CuInSe2 to 1.68?eV in CuGaSe2 by substituting indium for gallium. The addition of small amounts of Ga could not only raise the bandgap to more suitably match the AM1.5 solar spectrum, more importantly, but could also improve the electrical properties of CIGS films [9–11]. However, the CIGS films with higher gallium concentration show a significant loss of efficiency relative to lower gallium content films [12]. The optimal [Ga]/[In + Ga] ratio of CIGS absorber for high efficiency CIGS solar cell is considered to be 0.2~0.3 from a number of experimental results [13–15]. A variety of processing techniques have been developed to prepare high quality CIGS thin films. The two most reported processes are the coevaporation from elemental sources and the selenization of metallic precursors. The coevaporation process consists of three stages, which all need precise control over the deposition rate of each element. The selenization of metallic precursors is a two-stage process, which involves the deposition of Cu-In-Ga metallic precursors in the first step followed by their selenization using H2Se gas or Se vapor [16]. The presence of low-melting metal indium in Cu-In-Ga metallic precursors could always result in rough morphologies of the CIGS films and loss of indium from films during the ramp-up and selenization stages
DsHsp90 Is Involved in the Early Response of Dunaliella salina to Environmental Stress ?
Si-Jia Wang,Ming-Jie Wu,Xiang-Jun Chen,Yan Jiang,Yong-Bin Yan
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms13077963
Abstract: Heat shock protein 90 (Hsp90) is a molecular chaperone highly conserved across the species from prokaryotes to eukaryotes. Hsp90 is essential for cell viability under all growth conditions and is proposed to act as a hub of the signaling network and protein homeostasis of the eukaryotic cells. By interacting with various client proteins, Hsp90 is involved in diverse physiological processes such as signal transduction, cell mobility, heat shock response and osmotic stress response. In this research, we cloned the dshsp90 gene encoding a polypeptide composed of 696 amino acids from the halotolerant unicellular green algae Dunaliella salina. Sequence alignment indicated that DsHsp90 belonged to the cytosolic Hsp90A family. Further biophysical and biochemical studies of the recombinant protein revealed that DsHsp90 possessed ATPase activity and existed as a dimer with similar percentages of secondary structures to those well-studied Hsp90As. Analysis of the nucleotide sequence of the cloned genomic DNA fragment indicated that dshsp90 contained 21 exons interrupted by 20 introns, which is much more complicated than the other plant hsp90 genes. The promoter region of dshsp90 contained putative cis-acting stress responsive elements and binding sites of transcriptional factors that respond to heat shock and salt stress. Further experimental research confirmed that dshsp90 was upregulated quickly by heat and salt shock in the D. salina cells. These findings suggested that dshsp90 might serve as a component of the early response system of the D. salina cells against environmental stresses.
An efficient method for DNA extraction from compost
堆肥中微生物总DNA的高效提取

HE Li-hong,ZHAO Yong,CHEN Ming-jie,PAN Ying-jie,
何丽鸿
,赵勇,陈明杰,潘迎捷

微生物学报 , 2006,
Abstract: An efficient method for obtaining DNA from compost which contained high levels of organic matter was developed. The protocol consisted of washing with phosphate-EDTA before extraction, cell lysis with hot-SDS and enzymes (lysozyme, lywalzyme, proteinase K), removing humic acid and other inhibitors with PVPP and precipitation with PEG-8000. The compost total DNA was extracted from four different composts, the DNA yield was 63.54 +/- 12.08 to approximately 106.50 +/- 28.36 microg/g of dry compost. Molecular size of DNA obtained using this protocol was about 23kb and contained low protein and humic acid contamination with the A260/A280 ratios exceeding 1.6 and A260/A230 ratios reaching 1.8. Usually, additional purification steps such as agarose gel electrophoresis, gel permeation chromatography, or affinity chromatography were needed to get PCR-amplifiable DNA, but the DNA obtained using this protocol could directly be used to PCR-amplification and restriction enzyme digestion. Just like purity of DNA template, lower DNA yield also appears to introduce a bias towards lower community diversity. In this study compared the purified DNA the direct DNA reveals higher microbial community diversity assessed by denaturing gradient gel electrophoresis(DGGE) of amplified V3 region of 16S rDNA.
Distribution of a specific SCAR marker among Lentinula edodes protoplast monokaryons for strain 135
香菇135菌株特异SCAR标记在其原生质体单核中的分布

ZHANG Mei-Yan,TAN Qi,CHEN Ming-Jie,PAN Ying-Jie,
张美彦
,谭琦,陈明杰,潘迎捷

微生物学报 , 2008,
Abstract: Refrigerated mycelium and fresh fruitbody tissue-isolated mycelium of Lentinula edodes strain 135 were used to prepare protoplast monokaryons. Fifty-eight and 83 monokaryons were isolated respectively and their mating types were determined. PCR amplification employing specific primers was used to establish the distribution among the protoplast monokaryons of a SCAR marker specific for strain 135. Protoplast monokaryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The outcome is a regular distribution pattern that demonstrates the stable inheritance of the SCAR marker and is fundamental to identification of offspring arising from crosses between protoplast monokaryons of this particular mating type and other karyons.
SELECTION FOR COLD-RESISTENT STRAIN BY COMPLEX MUTAGENESIS OF VOLVARIELLA VOLVACEA AND IDENTIFICATION OF THE STRAIN
草菇耐低温菌株的诱变选育与鉴定

HAN Ye-Jun,CHEN Ming-Jie CAO Hui PAN Ying-Jie,
韩业君
,曹晖,陈明杰,潘迎捷

菌物学报 , 2004,
Abstract: 在草菇V23原生质体形成和再生的最佳条件下制备原生质体。分别用紫外线(UV),60Co-γ射线,硫酸二乙酯(DES)对原生质体进行复合诱变。初筛、复筛获得耐常规低温(4℃)10d的菌株VH。栽培实验证明:VH在26℃下的生物学效率明显优于V23(CK),且差异极显著。同工酶分析表明:VH过氧化物同工酶,酯酶同工酶谱带数量及酶活性均发生变化;RAPD实验显示:VH相对于V23的变异系数为0.213。VH菇体正常,味道鲜美,食用后无毒副作用。氨基酸组成分析表明:除总氨基酸和谷氨酸含量VH高于V23外,其它氨基酸差异不明显。VH遗传性状稳定,是具有潜在应用价值的优良菌株。
DETECTING IN 9 EXTRACELLULAR ENZYME ACTIVITIES OF AGROCYBE AEGERITA STRAINS
柱状田头菇生长发育中9种胞外酶活性的测定

WANG Nan,SHEN Feng,TAN Qi,CHEN Ming-Jie,PAN Ying-Jie,
王南
,沈锋,谭琦,陈明杰,潘迎捷

菌物学报 , 2000,
Abstract: 柱状田头菇Agrocybe aegerita作为一种新开发的食用菌,越来越受到消费者的喜爱和研究者的关注,但在其栽培生产中遇到的产量相对较低的问题,一直未能得到很好的解决,致使柱状田头菇的栽培难以得到大规模的推广。本研究对不同来源的柱状田头菇在其6个不同生长发育阶段中,9种与培养料中主要组分分解相关的胞外酶活性的变化进行了测定,结果表明:柱状田头菇属褐腐菌,对非木质纤维素的利用能力最强,对纤维素的利用能力较强,对木质素的利用能力较差,但柱状田头菇具有漆酶活性。
Cloning and sequence analysis of cold induced genes in Chinese straw mushroom, Volvariella volvacea
草菇冷诱导相关基因的克隆及序列分析

SUN Xiao-Hong,FENG Ai-Ping,CHEN Ming-Jie,PAN Ying-Jie,
孙晓红
,冯爱萍,陈明杰,潘迎捷

菌物学报 , 2006,
Abstract: 利用差异显示技术分离获得草菇低温特异DNA片段,经与正常草菇和低温诱导草菇cDNA分别southern杂交验证后,得到低温特异性片段。采用PCR标记技术对获得的低温特异性片段进行DIG标记,以此为探针,对低温处理的草菇cDNA文库进行筛选,获得4个阳性克隆,分别进行测序。序列同源性比较分析发现,Cor3基因与s-腺苷-L-高半胱氨酸水解酶有很高的同源性,Cor4基因与40S核糖体蛋白S9有很高的同源性,这两个基因可能与草菇的低温自溶现象有关。Cor1基因与脉孢菌的保守假设蛋白(conservedhypotheticalprotein)有同源性,Cor2基因与辅酶A连接酶有同源性。半定量RT-PCR验证发现Cor1和Cor2基因在正常情况下没有表达,低温处理后有表达,Cor3和Cor4基因在正常情况下有表达,低温处理后表达量增加。
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