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Search Results: 1 - 10 of 42503 matches for " CEN Ying-hua "
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Dissimilatory Fe (Ⅲ) Reduction and its Applications in Contaminants Treatment
异化Fe(Ⅲ)还原及其在污染治理中的作用

XU Mei-Ying,GUO Jun,CEN Ying-Hua,SUN Guo-Ping,
许玫英
,郭俊,岑英华,孙国萍

微生物学通报 , 2007,
Abstract: 细菌的异化Fe(Ⅲ)还原指以Fe(Ⅲ)为末端电子受体在无氧条件下氧化有机物的产能过程,在生物地球化学循环中起着重要的作用。系统综述了异化Fe(Ⅲ)还原细菌与多种代谢反应相耦联的Fe(Ⅲ)还原过程、还原不溶性Fe(Ⅲ)氧化物的机制,及其与Fe(Ⅲ)还原相关的分子生物学的研究进展。介绍了国内外有关Fe(Ⅲ)还原在环境污染治理中的研究现状及其发展趋势。
Study on the Biofilm Characteristics in Biotricking Filter Treating Odour Gas with Large Flow and Low Concentration
净化低浓度大风量恶臭气体的生物滴滤池中生物膜研究

ZHANG Tian-Tian,LI Jian-Jun,CEN Ying-Hua,SUN Guo-Ping,
张甜甜
,李建军,岑英华,孙国萍

微生物学通报 , 2007,
Abstract: 采用内装塑料片、塑料丝、海绵块的中空鱼网状塑料球为填料的生物滴滤池,对某垃圾压缩站产生的低浓度大风量的含氨臭气进行了近1年的连续脱臭试验。研究了有关的净化效果与生物膜特性。在进口氨气浓度0.8mg/m3~1.5mg/m3,风量8000m3/h,停留时间2.5s,氨气去除率为90%以上,达到国家一级排放水平。系统添加营养液时净化效果从75%提高到90%。3种填料的生物膜量、脱氢酶活性及硝化活性速率的排列顺序是:塑料片>塑料丝>海绵,并从工艺及填料方面探讨进一步提高净化效果的技术措施。
Recent Developments in Biological Waste Gas Purification
生物净化废气技术的进展

ZHANG Tian-Tian,LI Jian-Jun,CEN Ying-Hua,SUN Guo-Ping,
张甜甜
,李建军,岑英华,孙国萍

微生物学通报 , 2007,
Abstract: Biological treatment provides an cost effective and environmentally friendly alternative for many waste gas emissions.Biological waste air treatment is achieved at ambient temperatures,it does not generate secondary pollutants,such as nitrogen oxides,it has become in many instances the method of choice for the control of low concentrations of odors,volatile organic compounds,or hazardous air pollutants in large air streams.A significant body of knowledge and experience has been generated on biological waste gas purification.Examples of waste gas treatment are presented in this paper,such as at municipal wastewater treatment plants,emission from live stock industries,sulfur emissions from industries.This paper discusses the problem of how to prevent biomass clogging and introduces the application of molecular biotechnology in biologeical treatment.The development trend of the Biological treatment are discussed.We hope the biological treatments can gain widely application in China.
Study of Microbial Ecology in Biotrickling Filter Used to Treat Waste Air Containing Toluene
处理甲苯废气的生物滴滤池中微生物生态学研究

LI Jian-Jun,CHEN Jin-Lin,CEN Ying-Hua,SUN Guo-Ping,
李建军
,陈进林,岑英华,孙国萍

微生物学通报 , 2007,
Abstract: Denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rDNA was used to assess changes of biodiversity in a biotrickling filter used to treat waste air containing toluene. The results shown that along with gradually increase of removing capacity for toluene, microbial community structure in packing materials sampled from biotrickling filter also changed markedly over periods of experiment. Under selective pressure of toluene, the number of microbial species decreased but relative abundance of some predominant species increased,and microbial spatial location along the height of biotrickling filter tend to be identical.
Microbial community composition and phylogenetic analysis of deodor biofilter under different pH conditions
不同pH条件下除臭生物滤池微生物种群结构的分子生态分析

CHEN Tong-sheng,LI Jian-jun,CEN Ying-Hua,SUN Guo-ping,
陈桐生
,李建军,岑英华,孙国萍

微生物学报 , 2005,
Abstract: Molecular biological methods were applied to analyze the genetic diversity of microbe community in biofilter. V3 variable fragments of genes coding for 16S rRNA were amplified by Polymerase Chain Reaction. Then PCR-DGGE combined technique were used to analyze the microbial population composition and phylogeny of deodorant biofilter. The results indicate that there are prominent differences in microbial diversity and abundance between acid and neutral conditions. Owing to the selective pressure of extreme acidity, there is less diversity of microbe compared with that in neutral environment. It is demonstrated the obvious distinction of spacial diversity in different biofilter bed as well. PCR and T-vector cloning sequencing results shows that the sulfur-oxidizing bacteria are the predominant population. The research offers the valid scientific basis for better treatment of odorant gas and the theoretical foundation for application of bio-deodorization.
Properties of a triphenylmethane dyes decolorization enzyme (TpmD) from Aeromonas hydrophila strain DN322
细菌脱色酶TpmD的酶学特性研究

REN Sui-zhou,GUO Jun,CEN Ying-hua,SUN Guo-ping,
任随周
,郭俊,王亚丽,岑英华,孙国萍

微生物学报 , 2006,
Abstract: A novel bacterial enzyme for decolorization of triphenylmethane dyes from Aeromonas hydrophila strain DN322 was purified and named TpmD.The basic properties of this enzyme including molecular weight,isoelectric point Km as well as the optimum temperature and pH were determined and the enzyme was identified as an NADH/NADPH-dependent oxygenase in previous research.Based on previous results,the effect of different inhibitor including Vc,metyrapone,rotenone,antimycin A and NaN-3 as well as the effect of FAD and FMN on the activity of TpmD were measured.The results indicated that the activity of the decolorization enzyme was inhibited by Vc and metyrapone in a concentration-dependent manner,but wasn't inhibited by rotenone,antimycin A and NaN-3.The activity of the decolorization enzyme was not enhanced by addition of FAD or FMN.The solution of the enzyme protein displayed only a single peak at 408nm in the Soret region,a characteristic peak of porphyrin,but did not show the characteristic peak of the cytochrome P450 proteins at 450nm in sodium dithionite(DTN)-reduced enzyme solution after treatment with carbon monoxide.The amino acid sequence of N-terminal of TpmD provided further evidence that the enzyme is an oxygenase.All these results suggest that decolorization enzyme TpmD is a new hemo-containing oxygenase.The decolorization enzyme would be a good material for further research of the enzymological mechanism of triphenylmethane dyes decolorization by bacteria.
Recent Developments in Biological Waste Gas Purification
生物净化废气技术的进展

ZHANG Tian-Tian,LI Jian-Jun,CEN Ying-Hua,SUN Guo-Ping,
张甜甜
,李建军,岑英华,孙国萍

微生物学报 , 2007,
Abstract: Biological treatment provides an cost effective and environmentally friendly alternative for many waste gas emissions. Biological waste air treatment is achieved at ambient temperatures, it does not generate secondary pollutants, such as nitrogen oxides, it has become in many instances the method of choice for the control of low concentrations of odors, volatile organic compounds, or hazardous air pollutants in large air streams. A significant body of knowledge and experience has been generated on biological waste gas purification. Examples of waste gas treatment are presented in this paper, such as at municipal wastewater treatment plants, emission from live stock industries, sulfur emissions from industries. This paper discusses the problem of how to prevent biomass clogging and introduces the application of molecular biotechnology in biologeical treatment.The development trend of the Biological treatment are discussed.We hope the biological treatments can gain widely application in China.
Cloning and analysis of genes encoding 2-naphthoate monooxygenase and NADH: flavin oxidoreductase
2-萘酸加单氧酶基因及NADH:黄素还原酶基因的克隆与分析

LI Xiao-bo,CEN Ying-hua SUN Guo-ping,
李小波
,岑英华,孙国萍

微生物学报 , 2005,
Abstract: In Burkholderia sp.JT1500, a key step of 2-naphthoate biodegradation pathway is carried out by 2-naphthoate monooxygenase(Nmo) in which 2-naphthoate is oxidized to 1-hydroxy-2-naphthoate. A gene cluster of 4.8kb from Burkholderia sp. JT1500 was cloned and sequenced, four open reading frames named orfB, orfC, orfD and orfA were identified in this region. Sequence alignment showed that orfA had a high homology of nucleotide acid composition to monooxygenase genes from both Japonicum USDA 110 and Ralstonia eutropha HF 39, orfB had some homology to the component of flavin reductase genes from Bordetlla pertussis Tohama I, Ralstonia solanacearum GMI1000 and Bordetella bronchiseptica RB50.Enzyme activity analysis showed that the cell extracts of recombinant E.coli S- A (only harboring orfA) showed very low oxygenase oxidation activity as detected by NADH decreasing, while the cell extracts of recombinant S- B (only harboring orfB) did not show any oxidation activity at all. But when the cell extracts of S- B and S- A were mixed, which showed very strong oxidation activity when flavin (FMN or FAD) provided; the recombinant S- B A cells harboring both orfB and orfA genes also showed strong oxidation activity when flavin provided; weak flavin deoxidization activity could be detected from the cell extracts of E.coli S- B under anaerobic conditions. Based on above message, a conclusion was drawn that Nmo is consisted of two components: a flavin oxidoreductase (NmoB) and a monooxygenase (NmoA). First NmoB uses NADH to reduce flavin and supplies reduced flavin to NmoA to catalyze O- 2 oxidizing 2-NAT. NmoB is NmoA's coupling protein.
Study of Microbial Ecology in Biotrickling Filter Used to Treat Waste Air Containing Toluene
处理甲苯废气的生物滴滤池中微生物生态学研究

LI Jian-Jun,CHEN Jin-Lin,CEN Ying-Hua,SUN Guo-Ping,
李建军
,陈进林,岑英华,孙国萍

微生物学报 , 2007,
Abstract: 利用PCR-DGGE技术分析了处理甲苯废气的生物滴滤池生物多样性,结果表明:在运行过程中,随着生物滴滤池对甲苯去除能力的不断增加,填料当中的微生物种群也发生了明显的变化。在甲苯的选择压力下,随时间的迁延,微生物种类减少,优势种群的相对丰度增加,处于不同层面填料上的微生物分布也趋向于一致。
Application of stable carbon isotope techniques to research into water stress
碳稳定同位素技术在植物水分胁迫研究中的应用

CHEN Ying-Hua,
陈英华

生态学报 , 2004,
Abstract: 植物体的碳稳定同位素组成主要由植物本身的生物学特性决定 ,但环境胁迫对其影响也十分明显。综述了碳稳定同位素技术在研究植物水分利用效率、生物量高低及判断历史气候依据等研究领域的进展 ,阐明了植物体的 δ1 3C值对干旱、盐分及其它环境因素的变化所引起的水分胁迫的响应 ,并对碳稳定同位素对水分胁迫的响应机理进行了归纳和推断
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