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Search Results: 1 - 10 of 18321 matches for " CECILIA ANNA SEUMAHU "
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Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis
CECILIA ANNA SEUMAHU,ANTONIUS SUWANTO,IMAN RUSMANA,DEDY DURYADI SOLIHIN
HAYATI Journal of Biosciences , 2012,
Abstract: Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile.
The Dynamics of Bacterial Communities During Traditional Nata de Coco Fermentation
CECILIA ANNA SEUMAHU,ANTONIUS SUWANTO,DEBORA HADISUSANTO,MAGGY THENAWIJAYA SUHARTONO
Microbiology Indonesia , 2007,
Abstract: One of the important problems in traditional Nata de Coco (Nata) fermentation is production inconsistency due to strain or genetic variability reflecting mixed microbial communities involved in this process. This research was aimed at examine the population dynamics of the bacterial community during the fermentation processes. Samples were collected daily for six days from fermentation media derived from “good” and “bad” Nata fermentation. We compared the levels of bacterial diversity through amplified 16S-rRNA (ARDRA). DNA was extracted directly from the fermentation media and 16S-rRNA gene was amplified employing Universal Bacterial Primers. The amplicons were cloned into pGEM-T Easy vector, and restriction enzymes HaeIII and RsaI were used to generate ARDRA profiles. ARDRA phylotypes of DNA extracted from the fermentation medium obtained from different Nata qualities were compared. Phylotype profiles demonstrated unique bacterial community profiles for different conditions of Nata quality, which could be developed as a parameter to monitor Nata quality during fermentation. In this research we found that the dynamics of the bacterial population involved in Nata fermentation were a crucial factor for determining traditional Nata quality.
Chlamydia prevention in Sweden—A case study of potential key factors in successful response  [PDF]
Charlotte Deogan, Cecilia Moberg, Lene Lindberg, Anna M?nsdotter
Open Journal of Preventive Medicine (OJPM) , 2013, DOI: 10.4236/ojpm.2013.31009
Abstract: Background: After a continuous increase of Chlamydia trachomatis (chlamydia) in Sweden, a general reduction in reported cases was seen in 2009. However, the number and decrease of chlamydia cases varied largely between geographical regions. Aim: The aim of the present study was to identify potential key factors of successful regional prevention of chlamydia and other sexually transmitted infections (STIs). Methods: A multiple case study was performed including seven Swedish counties. Data was collected via surveys and interviews with key informants, county council registry data, survey data on condom use, and surveillance data on reported cases of chlamydia. In a case comparison, factors of prevention structure and prevention activities were identified and rated as strengths or weaknesses compared to standard preventive measures. Potential key factors were identified by examining prevention strengths corresponding to high condom use and decrease of chlamydia cases. Results: Differences were found in prevention structure and activities across counties. Identified potential key factors were; adequate investments in STI prevention, suitable organizational structure, strong leadership, managing regional STI-networks, research connection, multiple local collaborations with health care and community, high testing coverage and strategic risk approach.
The Rhythm of the Unit Is the Pace of Life: A Study of Everyday Activities and Sleep in Swedish Residential Care  [PDF]
Amanda Hellstr?m, Anna Condelius, Ania Willman, Cecilia Fagerstr?m
Open Journal of Nursing (OJN) , 2015, DOI: 10.4236/ojn.2015.58073
Abstract: Social and physical activities can improve sleep in older people. However, living in a residential care facility has been associated with a limited potential for activities and increased inactivity, reflected in poor sleep among residents. In turn, poor sleep can impair physical and mental functions. This paper explores sleep habits and everyday activities at three residential care facilities and investigates the link between sleep habits and everyday activities from the perspective of the residents. Data were collected through observations of daily life and interviews with residents. The results showed that fixed times during the day reduced daytime sleep and motivated them to go to the day room, thus enabling social interaction. More impaired residents spent more time in the day room napping or being less active. The residents stated that going outdoors was a desired activity, thought to improve sleep. However, the activity did not occur to the extent the residents wished for. Maintaining mobility and influence over daily activities together with regularity seemed to improve sleep. Awareness among staff of the need for stimulating and enriching activities, as well as access to bright light is requisite. Specific consideration should be given to residents who have difficulties communicating their wishes and/or impaired mobility.
Trapping-detrapping fluctuations in organic space-charge layers
Anna Carbone,Cecilia Pennetta,Lino Reggiani
Physics , 2010, DOI: 10.1063/1.3271769
Abstract: A trapping-detrapping model is proposed for explaining the current fluctuation behavior in organic semiconductors (polyacenes) operating under current-injection conditions. The fraction of ionized traps obtained from the current-voltage characteristics, is related to the relative current noise spectral density at the trap-filling transition. The agreement between theory and experiments validates the model and provides an estimate of the concentration and energy level of deep traps.
Applicability of in Vitro Models in Predicting the in Vivo Bioavailability of Lycopene and β-Carotene from Differently Processed Soups  [PDF]
Marie Alminger, Cecilia Svelander, Anna Wellner, Rebeca Martinez-Tomas, Lucy Bialek, Elvira Larque, Francisca Perez-Llamas
Food and Nutrition Sciences (FNS) , 2012, DOI: 10.4236/fns.2012.34068
Abstract: Presently, there is no clear consensus on the best approach to estimate carotenoid bioavailability. The best alternative would be to use human studies, but they are labour-intensive and expensive and can only be used to investigate a limited number of samples. Hence, a number of in vitro: models have been developed to study pre-absorptive processes and factors affecting bioavailability. The question is, however, how well the results obtained by the various methods correlate to each other and to the in vivo situation. In the present paper, we have compared in vivo data from two human studies on differently processed soups containing carrots, tomato and broccoli, with results obtained by in vitro characterisation of the same soups. In vitro bioaccessibility was estimated by a static in vitro digestion investigating matrix release and micellarization of carotenoids and by uptake studies in a human intestinal cell line (Caco-2). In vivo data was obtained from clinical studies measuring total plasma carotenoid concentrations in human subjects after 4 weeks daily consumption of the soups. Comparison of the in vitro and in vivo results indicate that the combination of a two-step in vitro digestion and Caco-2 cells seems to be a useful tool for estimation of β-carotene bioaccessibility and screening of factors governing the release of β-carotene from this type of food. For lycopene the in vitro and in vivo results were less consistent, suggesting that reliable prediction of lycopene bioavailability might be more problematic.
Nutrient Analysis Critical Control Point (NACCP): Hazelnut as a Prototype of Nutrigenomic Study  [PDF]
Laura Di Renzo, Alberto Carraro, Daniela Minella, Roberto Botta, Cecilia Contessa, Chiara Sartor, Anna Maria Iacopino, Antonino De Lorenzo
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.51011
Abstract:

The aim of this study is to apply the Nutrient Analysis Critical Control Point (NACCP) process to ensure that the highest nutrient levels in food can determine a beneficial effect on the health of the consumer. The NACCP process involves a sequence of analysis and controls that depart from raw material production to the evaluation of the effect of nutrition on health. It is articulated through the following points: 1) identification of nutrient level in the food; 2) identification of critical control points (environmental, genetic data, chemical and physical data, production technology, distribution and administration); 3) establishing critical limits that can impoverish and damage the nutrient; 4) establishing measures to monitor; 5) establishing corrective actions. We selected as biomarkers the total phenolic content (TPC) and total antioxidant capacity (TAC) of a genotyped Italian hazelnut cultivars (Corylus e avellana L.). We performed a clinical study evaluating: a) nutritional status; b) clinical-biochemical parameters; c) low density lipoprotein oxidation (LDL-ox); d) the expression level changes of oxidative stress pathway genes in the blood cell at baseline and after 40 g/die of hazelnut consumption. In this study, we found a significant lowering (p ≤ 0.005) of LDL oxidized proteins, in association with the consumption of 40 g/d of hazelnuts. Also, we found a significant variation (p ≤ 0.005) of gene expression of antioxidant and pro-oxidant genes, between the intake of dietary with and without hazelnuts. This results support the hypothesis that the NACCP process could be applied to obtain significant benefits in terms of primary prevention and for contributing to the amelioration of food management at the consumer level.

The 2011 WPATH Standards of Care and Penile Reconstruction in Female-to-Male Transsexual Individuals
Gennaro Selvaggi,Cecilia Dhejne,Mikael Landen,Anna Elander
Advances in Urology , 2012, DOI: 10.1155/2012/581712
Abstract: The World Professional Association for Transgender Health (WPATH) currently publishes the Standards of Care (SOC), to provide clinical guidelines for health care of transsexual, transgender and gender non-conforming persons in order to maximize health and well-being by revealing gender dysphoria. An updated version (7th version, 2011) of the WPATH SOC is currently available. Differences between the 6th and the 7th versions of the SOC are shown; the SOC relevant to penile reconstruction in female-to-male (FtM) persons are emphasized, and we analyze how the 2011 WPATH SOC is influencing the daily practice of physicians involved in performing a penile reconstruction procedure for these patients. Depending by an individual’s goals and expectations, the most appropriate surgical technique should be performed: the clinic performing penile reconstruction should be able to offer the whole range of techniques, such as: metoidioplasty, pedicle and free flaps phalloplasty procedures. The goals that physicians and health care institutions should achieve in the next years, in order to improve the care of female-to-male persons, consist in: informing in details the individuals applying for penile reconstruction about all the implications; referring specific individuals to centers capable to deliver a particular surgical technique; implementing the surgery with the most updated refinements. 1. Background 1.1. Gender Dysphoria Gender dysphoria (GD) refers to discomfort or distress that is caused by a discrepancy between a person’s gender identity and that person’s sex assigned at birth (and the associated gender role and/or primary and secondary sex characteristics) [1, 2]. When the gender dysphoria reaches a significant level of distress, it can meet the criteria for a formal diagnosis: transsexualism in ICD 10 or gender identity disorder (GID) in DSM IV [3, 4]. The diagnosis is based on the person’s description of incongruence between gender identity and phenotype, and the assessment of this by mental health professionals. There is currently a political movement against inclusion of transsexualism in diagnostic manuals in order not to view transsexualism as a psychiatric disorder, or as a diagnosis per se. The delineation of conditions involving GD started in Germany in the 19th century [5], but it was Harry Benjamin’s publication “The transsexual phenomenon” on the subject in 1966 that led to the widespread use of the term transsexualism [6]; the first diagnostic criteria were outlined in 1968. He also proposed a treatment protocol for transsexual persons who
Orbital Dynamics of a Simple Solar Photon Thruster
Anna D. Guerman,Georgi V. Smirnov,Maria Cecilia Pereira
Mathematical Problems in Engineering , 2009, DOI: 10.1155/2009/537256
Abstract: We study orbital dynamics of a compound solar sail, namely, a Simple Solar Photon Thruster and compare its behavior to that of a common version of sailcraft. To perform this analysis, development of a mathematical model for force created by light reflection on all sailcraft elements is essential. We deduce the equations of sailcraft's motion and compare performance of two schemes of solar propulsion for two test time-optimal control problems of trajectory transfer.
Implications of Advancing Paternal Age: Does It Affect Offspring School Performance?
Anna C. Svensson, Kathryn Abel, Christina Dalman, Cecilia Magnusson
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024771
Abstract: Average paternal age is increasing in many high income countries, but the implications of this demographic shift for child health and welfare are poorly understood. There is equivocal evidence that children of older fathers are at increased risk of neurodevelopmental disorders and reduced IQ. We therefore report here on the relationship between paternal age and a composite indicator of scholastic achievement during adolescence, i.e. compulsory school leaving grades, among recent birth cohorts in Stockholm County where delayed paternity is notably common. We performed a record-linkage study comprising all individuals in Stockholm County who finished 9 years of compulsory school from 2000 through 2007 (n = 155,875). Data on school leaving grades and parental characteristics were retrieved from administrative and health service registers and analyzed using multiple linear regression. Advancing paternal age at birth was not associated with a decrease in school leaving grades in adolescent offspring. After adjustment for year of graduation, maternal age and parental education, country of birth and parental mental health service use, offspring of fathers aged 50 years or older had on average 0.3 (95% CI ?3.8, 4.4) points higher grades than those of fathers aged 30–34 years. In conclusion, advancing paternal age is not associated with poorer school performance in adolescence. Adverse effects of delayed paternity on offspring cognitive function, if any, may be counterbalanced by other potential advantages for children born to older fathers.
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