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Search Results: 1 - 10 of 12194 matches for " CAO Weidong "
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Homologous Rearranged DNA can Change Phenotype and Genotype of the Host by Transgenic Method and a QTL Related to Weight was Obtained from it
Zheming Cao,Weidong Ding
Advance Journal of Food Science and Technology , 2013,
Abstract: The research study aim at looking for a simple way to obtain mutant while know what change in the genome of the host. We rearrange carp genomic DNA by digestion, ligation and addition of adaptor and then transferred the homologous rearranged DNA into carp eggs. The results showed that introduction of the homologous rearranged DNA slightly decreased the hatchability of fertilized eggs. PCR products with primers against adaptors amplified from offspring carps had different sizes compared with those amplified from the parent carps, indicating that shuffled genomic DNA has been incorporated into the genomes of offspring. Different size of PCR fragments were obtained after amplification of DNA from two small-size carps that has ceased to develop. Four clones of introducing DNA were sequenced and most of them were microsatellite DNA. Based on one of these sequences, we designed three forward and one reverse primer to amplify the genomic DNA from normal carps and we found that the amplified sequences were homologous rearranged DNA. Four transgenic fish with large body weight were selected as the father and hybridized with common female carp. We gained four groups of offspring. The muscle tissue was chosen as the sample for amplification of introducing DNA fragments. The separation of introducing DNA in three groups is confusing but clear in one group. Further analysis on the group with clear separation shows that the introducing sequence can make the weight of the host drift to the large direction and lower the differentiation between individuals with such sequence. The sequence has no coding function and no region similar to the known regulatory sequence. The study shown that the homologous rearranged DNA can be integrated into the genome of the host and make impact on the host both in genotype and phenotype.
Efficacy and Safety of Dexmedetomidine during Anesthesia Induction of Patients with Intracranial Tumor: A Preliminary Observational Trial  [PDF]
Jiangbei Cao, Wenzhu Shi, Weidong Mi, Hong Zhang
Pharmacology & Pharmacy (PP) , 2013, DOI: 10.4236/pp.2013.48084
Abstract: Background: The efficacy and safety of dexmedetomidine during the anesthesia induction of intracranial tumor patients remain unknown. We wondered whether loading infusion of dexmedetomidine 1 μg/kg over 10 min to intracranial tumor patients was as efficient and safe as to those abdominal disease patients. Methods: Patients aged 18-60 years, male or female, ASA I or II, scheduled for intracranial tumor resection (Group N, n = 30) or abdominal operation (Group A, n = 30) were enrolled in this observational trial. Dexmedetomidine was administrated with a loading dosage of 1 μg/kg over 10 min following with continuous infusing of 0.5 μg/kg/h. Fentanyl, propofol and rocuronium were sequentially administered for anesthesia induction. Heart rate (HR), blood pressure (BP), pulse oxygen saturation (SpO2), bispectral index (BIS) and other adverse effects were recorded from the beginning of loading infusion of dexmedetomidine to the end of endotracheal intubation. Results: Among with loading infusion, HR and BIS value decreased and were significantly lower at the end of infusion than before infusion (P < 0.01), but BP did not (P > 0.05). One patient of Group N dropped out from this trial because of a serious headache. 14 of 29 patients during dexmedetomidine loading infusion suffered hypoxemia (SpO2 < 90%) in Group N, which was higher than 6 of 30 of in Group A (P < 0.05). No other side effects were recorded.
GCS overexpression is associated with multidrug resistance of human HCT-8 colon cancer cells
Min Song, Weidong Zang, Baohua Zhang, Jing Cao, Guanrui Yang
Journal of Experimental & Clinical Cancer Research , 2012, DOI: 10.1186/1756-9966-31-23
Abstract: The cell proliferation and cell toxicity were measured with Cell Counting Kit-8 (CCK-8). The mRNA levels of GCS and MDR1 were detected by semiquantitative reverse transcription-PCR amplification, the protein levels of GCS, caspase-3 and P-gp proteins were indicated by Western blotting. The apoptosis rates of cells were measured with flow cytometry.The relative mRNA levels of GCS in HCT-8, HCT-8/VCR, HCT-8/VCR- sh-mock and HCT-8/VCR-sh-GCS were 71.4 ± 1.1%, 95.1 ± 1.2%, 98.2 ± 1.5%, and 66.6 ± 2.1% respectively. The mRNA levels of MDR1 were respectively 61.3 ± 1.1%, 90.5 ± 1.4%, 97.6 ± 2.2% and 56.1 ± 1.2%. The IC50 of Cisplatin complexes were respectively 69.070 ± 0.253 μg/ml, 312.050 ± 1.46 μg/ml, 328.741 ± 5.648 μg/ml, 150.792 ± 0.967 μg/ml in HCT-8, HCT-8/VCR, HCT-8/VCR-sh-mock and HCT-8/VCR-sh-GCS. The protein levels of caspase-3 were 34.2 ± o.6%, 93.0 ± 0.7%, 109.09 ± 0.7%, 42.7 ± 1.3% respectively. The apoptosis rates of cells were 8.77 ± 0.14%, 12.75 ± 0.54%, 15.39 ± 0.41% and 8.49 ± 0.23% respectively.In conclusion, our research indicated that suppression of GCS restores the sensitivity of multidrug resistance colon cancer cells to drug treatment.Multidrug resistance (MDR) is one of the main impediments to the successful treatment of colon cancer [1]. Furthermore, colorectal tumors which obtain resistance to one drug are often resistant to several other drugs as well [2]. The underlying mechanisms are complicated [3]. One reason for MDR relates to P-glycoprotein (P-gp) and other transporters which are expressed in some cancer cells and could strengthen the efflux of diverse chemotherapeutic agents from cells [2]. Elevated levels of these MDR proteins, which belong to the ATP-binding cassette (ABC) transporter family, strengthen cellular efflux and reduce the effectiveness of anticancer drugs [4]. One method to measure P-glycoprotein efflux has been set up to o determine tumor response to chemotherapy [1]. To conquer drug resistance, inhibitors of MDR protein
Plasmid-encoded NP73-102 modulates atrial natriuretic peptide receptor signaling and plays a critical role in inducing tolerogenic dendritic cells
Zhang Weidong,Cao Xueqin,Chen Dongqing,Wang Jia-wang
Genetic Vaccines and Therapy , 2011, DOI: 10.1186/1479-0556-9-3
Abstract: Background Atrial natriuretic peptide (ANP) is an important endogenous hormone that controls inflammation and immunity by acting on dendritic cells (DCs); however, the mechanism remains unclear. Objective We analyzed the downstream signaling events resulting from the binding of ANP to its receptor, NPRA, and sought to determine what aspects of this signaling modulate DC function. Methods We utilized the inhibitory peptide, NP73-102, to block NPRA signaling in human monocyte-derived DCs (hmDCs) and examined the effect on DC maturation and induced immune responses. The potential downstream molecules and interactions among these molecules involved in NPRA signaling were identified by immunoprecipitation and immunoblotting. Changes in T cell phenotype and function were determined by flow cytometry and BrdU proliferation ELISA. To determine if adoptively transferred DCs could alter the in vivo immune response, bone marrow-derived DCs from wild-type C57BL/6 mice were incubated with ovalbumin (OVA) and injected i.v. into C57BL/6 NPRA-/- knockout mice sensitized and challenged with OVA. Lung sections were stained and examined for inflammation and cytokines were measured in bronchoalveolar lavage fluid collected from parallel groups of mice. Results Inhibition of NPRA signaling in DCs primes them to induce regulatory T cells. Adoptive transfer of wild type DCs into NPRA-/- mice reverses the attenuation of lung inflammation seen in the NPRA-knockout model. NPRA is associated with TLR-2, SOCS3 and STAT3, and inhibiting NPRA alters expression of IL-6, IL-10 and TGF-β, but not IL-12. Conclusions Modulation of NPRA signaling in DCs leads to immune tolerance and TLR2 and SOCS3 are involved in this induction.
The Regularized Low Pass Filter  [PDF]
Weidong Chen
Journal of Signal and Information Processing (JSIP) , 2014, DOI: 10.4236/jsip.2014.51003

In this paper the low pass filter is discussed in the noisy case. And a regularized low pass filter is presented. The convergence property of the regularized low pass filtering algorithm is proved in theory and tested by numerical results.

Soft Bipolar Depression Progress in China  [PDF]
Weidong Jin
Open Journal of Depression (OJD) , 2015, DOI: 10.4236/ojd.2015.44006
Abstract: Object: To introduce soft bipolar progress in China. Methods: We introduced soft bipolar concept into Chinese psychiatry and some studies about soft bipolar had been carried out by Chinese psychiatrist according to our soft bipolar criteria. Results: These studies include as following: 1) the proportion of bipolar disorder with depressive episode for the first time; 2) unipolar depression and bipolar depression compared in psychopathology; 3) the difference in personality and temperament between unipolar and bipolar; 4) family history of bipolar disorder; 5) antidepressants and soft bipolar. All these were used for establishing Chinese advising diagnostic criteria of soft bipolar disorder. It indicated that concept of soft bipolar was not only receipted, but also studied. Conclusion: Some progress of soft bipolar in China has been done.
Resource Transaction and the Disadvantage of Vulnerable Youth  [PDF]
Weidong Wu
Chinese Studies (ChnStd) , 2018, DOI: 10.4236/chnstd.2018.71008
Abstract: Under the background of social transition and globalization, vulnerable youth in China is quite disadvantaged in the public activities. Based on the ecological system perspective, this paper examines the situation of vulnerable youth in Tianjin, one of the five municipalities directly under the Central Government in China, by the qualitative-quantitative mixed method. Findings show that the disadvantage of vulnerable youth is caused by the shortage of economy resource, culture resource and information resource, which the groups can not obtain sufficiently from family system, school system, work system and community system by successful transaction. Some suggestions for social policies and further research are set forth accordingly.
Intestinal Absorption and First-Pass Metabolism of Polyphenol Compounds in Rat and Their Transport Dynamics in Caco-2 Cells
Zenghui Teng, Chengjun Yuan, Feng Zhang, Menglei Huan, Weidong Cao, Kangchu Li, Jingyue Yang, Dayong Cao, Siyuan Zhou, Qibing Mei
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029647
Abstract: Background Polyphenols, a group of complex naturally occurring compounds, are widely distributed throughout the plant kingdom and are therefore readily consumed by humans. The relationship between their chemical structure and intestinal absorption, transport, and first-pass metabolism remains unresolved, however. Methods Here, we investigated the intestinal absorption and first-pass metabolism of four polyphenol compounds, apigenin, resveratrol, emodin and chrysophanol, using the in vitro Caco-2 cell monolayer model system and in situ intestinal perfusion and in vivo pharmacokinetic studies in rats, so as to better understand the relationship between the chemical structure and biological fate of the dietary polyphenols. Conclusion After oral administration, emodin and chrysophanol exhibited different absorptive and metabolic behaviours compared to apigenin and resveratrol. The differences in their chemical structures presumably resulted in differing affinities for drug-metabolizing enzymes, such as glucuronidase and sulphatase, and transporters, such as MRP2, SGLT1, and P-glycoprotein, which are found in intestinal epithelial cells.
The Total Shift and Evolution of the Yangze River Delta Container Port System

LIANG Shuangbo,CAO Youhui,CAO Weidong,WU Wei,

地理科学进展 , 2008,
Abstract: It is interesting to examine the validity of the Hayuth model in the context of the Yangtze River Delta,especially when considering the differences between the coastal container port ranges and rivers container port ranges or different port categories.In this paper,a distinction between small ports(average container traffic for the period 1990-2006 of less than 100000TEU),medium-sized ports(between 100000 and 400000TEU) and large container ports(at least 400000TEU).Then,share-shift analysis models are used to measure the total shift and the evolution of the Yangtze River Delta container port system from 1990 to 2006.By calculating the concerned statistics,the author holds that there are four obvious features:(1) From 1994 to 1998,Shanghai was one of the major winners in terms of the total shift,but in the other periods,it lost some TEU.(2)Ningbo port has been the major winner in terms of the total shift since 1990 and shows the best performances in the period of 1990-1994 and 1998-2006.(3)In the mass,the coastal container port range is superiorior,along rivers container port range is inferior.The net volume of containers shifted between the respective ranges reached an expectionally high level in the third period.In this period the coastal container port range won a potential growth of approximately 607046 TEU to ports situated in the other ranges.(4)In competition,large container port ranges have won much TEU since 1994;middle container port ranges lost throughout some TEU(from a positive total shift of 22109TEU in the first period to a negative shift in the last);small container port ranges have won much TEU since 1990.A systematic analysis has been carried out on the formation of the total shift change.Finally,this paper holds that there are three development stages for this container port system:initial container port development,hub or load center container port and large deep-sea direct container port.
Therapeutic Effects of Astragaloside IV on Myocardial Injuries: Multi-Target Identification and Network Analysis
Jing Zhao, Pengyuan Yang, Fan Li, Lin Tao, Hong Ding, Yaocheng Rui, Zhiwei Cao, Weidong Zhang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044938
Abstract: Astragaloside IV (AGS-IV) is a main active ingredient of Astragalus membranaceus Bunge, a medicinal herb used for cardiovascular diseases (CVD). In this work, we investigated the therapeutic mechanisms of AGS-IV at a network level by computer-assisted target identification with the in silico inverse docking program (INVDOCK). Targets included in the analysis covered all signaling pathways thought to be implicated in the therapeutic actions of all CVD drugs approved by US FDA. A total of 39 putative targets were identified. Three of these targets, calcineurin (CN), angiotensin-converting enzyme (ACE), and c-Jun N-terminal kinase (JNK), were experimentally validated at a molecular level. Protective effects of AGS-IV were also compared with the CN inhibitor cyclosporin A (CsA) in cultured cardiomyocytes exposed to adriamycin. Network analysis of protein-protein interactions (PPI) was carried out with reference to the therapeutic profiles of approved CVD drugs. The results suggested that the therapeutic effects of AGS-IV are based upon a combination of blocking calcium influx, vasodilation, anti-thrombosis, anti-oxidation, anti-inflammation and immune regulation.
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