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Exérese do segmento vertical do canalículo lacrimal na síndrome do olho seco: estudo preliminar
Forno, Eliana;Buzalaf, Flavio;
Arquivos Brasileiros de Oftalmologia , 2005, DOI: 10.1590/S0004-27492005000200009
Abstract: purpose: to demonstrate the efficacy and possible complications of a surgical technique that includes the removal of the vertical portion of the lacrimal canaliculus in patients with dry eye syndrome. methods: a study was performed on six canaliculi of six eyes (four patients). three patients had dry eye, associated with primary sj?gren syndrome. one of the four patients developed keratoconjuntivitis sicca due to lacrimal gland removal. the criteria included: patients with symptoms of dry eye that did not improve even with the continuous use of artificial tears, low results (less than 5 mm) with the schirmer test, rose bengal staining and cases of recanalization after thermal occlusion with electrocauterium. the lacrimal puncta were examined after 7, 15, 30, 60, 90 and 180 days after surgery. results: none of the canaliculus recanalized at this time. punctate keratitis, corneal filaments, schirmer and rose bengal tests improved in 5 eyes. difuse punctate keratitis was present in the patient with lacrimal gland removal even 2 months after of surgery. eyelid margin complications were not observed. conclusion: this technique is simple and effective in permanent lacrimal canaliculus occlusion. we did not note any complications compared to other procedures.
O aniversário do Pasquim: 22 anos em 5
Márcia Neme Buzalaf
Discursos Fotográficos , 2009,
Abstract: Resenha: Pasquim 40 anos: Edi o Comemorativa. Rio de Janeiro: Desiderata, 2009, 40 p. e Antologia do Pasquim - volume III:1973-1974, de Jaguar e Sérgio Augusto (Org.). Rio de Janeiro: Desiderata, 2009, 376 p.
Coriorretinite esclopetária: relato de casos
Buzalaf, Flavio;Helal Jr, John;Nakashima, Yoshitaka;
Arquivos Brasileiros de Oftalmologia , 2003, DOI: 10.1590/S0004-27492003000100018
Abstract: the purpose of this paper is to warn the ophthalmologist that he/she may be facing a case of chorioretinitis sclopetaria when a patient presents with trauma due to shotgun reaching the orbit. three cases, two female and one male, with ages between 15 and 25 years, assisted at the emergency room of the "hospital das clínicas" of the university of s?o paulo, are described.
A fluoreta??o da água de abastecimento público e seus benefícios no controle da cárie dentária: cinqüenta anos no Brasil
Ramires,Irene; Buzalaf,Marília Afonso Rabelo;
Ciência & Saúde Coletiva , 2007, DOI: 10.1590/S1413-81232007000400027
Abstract: fluoridation of public water supplies is among the most important public health measures for control of dental caries. through a review of the literature, this study intends to reaffirm the importance and scope of fluoridation for caries control, as this is acknowledged as one of the most effective ways of ensuring the constant presence of fluoride in the oral cavity, which is vital for controlling caries. water fluoridation is rated as an important factor for reducing caries, meaning that it should be maintained and also monitored, ensuring adequate fluoride levels for controlling caries while avoiding dental fluorosis.
Coriorretinite esclopetária: relato de casos
Buzalaf Flavio,Helal Jr John,Nakashima Yoshitaka
Arquivos Brasileiros de Oftalmologia , 2003,
Abstract: O objetivo deste trabalho é prevenir o oftalmologista, ao se deparar com trauma por projétil de arma de fogo atingindo a órbita, de que é possível estar diante de um quadro de coriorretinite esclopetária. Foram descritos três casos, dois do sexo feminino e um do masculino, com idades variando entre 15 e 25 anos, atendidos no pronto-socorro do Hospital das Clínicas da Faculdade de Medicina da Universidade de S o Paulo.
Iron supplementation reduces the erosive potential of a cola drink on enamel and dentin in situ
Kato, Melissa Thiemi;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2012, DOI: 10.1590/S1678-77572012000300004
Abstract: iron has been suggested to reduce the erosive potential of cola drinks in vitro.objective: the aim of this study was to evaluate in situ the effect of ferrous sulfate supplementation on the inhibition of the erosion caused by a cola drink. material and methods: ten adult volunteers participated in a crossover protocol conducted in two phases of 5 days, separated by a washout period of 7 days. in each phase, they wore palatal devices containing two human enamel and two human dentin blocks. the volunteers immersed the devices for 5 min in 150 ml of cola drink (coca-colatm, ph 2.6), containing ferrous sulfate (10 mmol/l) or not (control), 4 times per day. the effect of ferrous sulfate on the inhibition of erosion was evaluated by profilometry (wear). data were analyzed by paired t tests (p<0.05). results: the mean wear (±se) was significantly reduced in the presence of ferrous sulfate, both for enamel (control: 5.8±1.0 μm; ferrous sulfate: 2.8±0.6 μm) and dentin (control: 4.8±0.8 μm; ferrous sulfate: 1.7±0.7 μm). conclusions: the supplementation of cola drinks with ferrous sulfate can be a good alternative for the reduction of their erosive potential. additional studies should be done to test if lower ferrous sulfate concentrations can also have a protective effect as well as the combination of ferrous sulfate with other ions.
Effect of one-bottle adhesive systems on the fluoride release of a resin-modified glass ionomer
Wang, Linda;Buzalaf, Marília Afonso Rabelo;Atta, Maria Teresa;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000100003
Abstract: a dhesive systems associated to resin-modified glass ionomer cements are employed for the achievement of a higher bond strength to dentin. despite this benefit, other properties should not be damaged. this study aimed at evaluating the short-time fluoride release of a resin-modified glass ionomer cement coated with two one-bottle adhesive systems in a ph cycling system. four combinations were investigated: g1: vitremer (v); g2: vitremer + primer (vp); g3: vitremer + single bond (vsb) and g4: vitremer + prime & bond 2.1 (vpb). sb is a fluoride-free and pb is a fluoride-containing system. after preparation of the vitremer specimens, two coats of the selected adhesive system were carefully applied and light-cured. specimens were immersed in demineralizing solution for 6 hours followed by immersion in remineralizing solution for 18 hours, totalizing the 15-day cycle. all groups released fluoride in a similar pattern, with a greater release in the beginning and decreasing with time. vp showed the greatest fluoride release, followed by v, with no statistical difference. vsb and vpb released less fluoride compared to v and vp, with statistical difference. regardless the one-bottle adhesive system, application of coating decreased the fluoride release from the resin-modified glass ionomer cements. this suggests that this combination would reduce the beneficial effect of the restorative material to the walls around the restoration.
Uso da fáscia temporal na suspens o frontal: descri o da técnica cirúrgica - Relato de caso
Nunes Tania Pereira,Sardinha Mariluze,Cardoso Laura,Buzalaf Flávio
Arquivos Brasileiros de Oftalmologia , 2004,
Abstract: OBJETIVOS: Familiarizar o oftalmologista com a anatomia da regi o temporal, descrever a técnica cirúrgica da retirada da fáscia temporal e da suspens o frontal e analisar as vantagens e desvantagens da fáscia temporal na suspens o frontal. MéTODOS: Revis o do prontuário de uma paciente com blefaroptose grave que foi submetida à suspens o frontal com fáscia temporal. Revis o da anatomia da fossa temporal e das técnicas cirúrgicas. RESULTADOS: Bom resultado estético e funcional foi conseguido no caso descrito. CONCLUS O: A fáscia temporal é boa op o na suspens o frontal com algumas vantagens: é um tecido autógeno, de fácil obten o e mínima morbidade no pós-operatório.
Renal Proteome in Mice with Different Susceptibilities to Fluorosis
Juliane Guimar?es Carvalho, Aline de Lima Leite, Camila Peres-Buzalaf, Fernanda Salvato, Carlos Alberto Labate, Eric T. Everett, Gary Milton Whitford, Marília Afonso Rabelo Buzalaf
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0053261
Abstract: A/J and 129P3/J mouse strains have different susceptibilities to dental fluorosis due to their genetic backgrounds. They also differ with respect to several features of fluoride (F) metabolism and metabolic handling of water. This study was done to determine whether differences in F metabolism could be explained by diversities in the profile of protein expression in kidneys. Weanling, male A/J mice (susceptible to dental fluorosis, n = 18) and 129P3/J mice (resistant, n = 18) were housed in pairs and assigned to three groups given low-F food and drinking water containing 0, 10 or 50 ppm [F] for 7 weeks. Renal proteome profiles were examined using 2D-PAGE and LC-MS/MS. Quantitative intensity analysis detected between A/J and 129P3/J strains 122, 126 and 134 spots differentially expressed in the groups receiving 0, 10 and 50 ppmF, respectively. From these, 25, 30 and 32, respectively, were successfully identified. Most of the proteins were related to metabolic and cellular processes, followed by response to stimuli, development and regulation of cellular processes. In F-treated groups, PDZK-1, a protein involved in the regulation of renal tubular reabsorption capacity was down-modulated in the kidney of 129P3/J mice. A/J and 129P3/J mice exhibited 11 and 3 exclusive proteins, respectively, regardless of F exposure. In conclusion, proteomic analysis was able to identify proteins potentially involved in metabolic handling of F and water that are differentially expressed or even not expressed in the strains evaluated. This can contribute to understanding the molecular mechanisms underlying genetic susceptibility to dental fluorosis, by indicating key-proteins that should be better addressed in future studies.
Prostaglandin and the Suppression of Phagocyte Innate Immune Responses in Different Organs
Alexandra Medeiros,Camila Peres-Buzalaf,Felipe Fortino Verdan,C. Henrique Serezani
Mediators of Inflammation , 2012, DOI: 10.1155/2012/327568
Abstract: The local and systemic production of prostaglandin E2 (PGE2) and its actions in phagocytes lead to immunosuppressive conditions. PGE2 is produced at high levels during inflammation, and its suppressive effects are caused by the ligation of the E prostanoid receptors EP2 and EP4, which results in the production of cyclic AMP. However, PGE2 also exhibits immunostimulatory properties due to binding to EP3, which results in decreased cAMP levels. The various guanine nucleotide-binding proteins (G proteins) that are coupled to the different EP receptors account for the pleiotropic roles of PGE2 in different disease states. Here, we discuss the production of PGE2 and the actions of this prostanoid in phagocytes from different tissues, the relative contribution of PGE2 to the modulation of innate immune responses, and the novel therapeutic opportunities that can be used to control inflammatory responses. 1. General Considerations Prostaglandins (PGs) are lipid mediators derived from arachidonic acid (AA) metabolism via the activation of the cyclooxygenase (COX) pathway, that regulates inflammation, immune response, hematopoiesis, tissue injury and repair, and bone resorption. PGs are found in most tissues and organs, and the variety of effects that they can elicit reflects the presence of specific PG receptors in many cell types. Upon cell activation by microbial products, cytokines, and opsonins, cytosolic phospholipase A2 (PLA2) is activated and recruited to hydrolase plasma cell phospholipids. Once it is released from the membrane, AA is rapidly converted into PGs by cells expressing prostaglandin H synthase (COX). At least two COX isoforms exist, the constitutive (COX-1) and inducible (COX-2) isoforms. COX-1 is expressed in many cell types distributed throughout the body, whereas COX-2 expression is highly restricted under basal conditions and upregulated during inflammation in different cell types [1] (see Figure 1). COX proteins are the major targets of nonsteroidal anti-inflammatory drugs (NSAIDs). Figure 1: Prostanoid biosynthesis and receptors. Upon cell stimulation, PLA2 is activated, and (AA) is released from the cellular membranes. AA is then metabolized by COX-1 or COX-2 in different cellular compartments and further metabolized by different synthases, which leads to the generation of different prostanoids. Once the product is formed, different prostanoids are transported outside the cells to bind to their respective receptors. (PG prostaglandin; Tx thromboxane; P G J 2 15-deoxy-Δ 12,14-prostaglandin J 2 ; Cox-1/2 cyclooxygenase-1/2; PGDS, PGES,
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