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Search Results: 1 - 7 of 7 matches for " Busarawan Sriwanthana "
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A phase I trial of Gynostemma pentaphyllum Makino in healthy volunteers
Pranee Chavalittumrong,Busarawan Sriwanthana,Rungrueng Kijphati,Bongkod Jitjuk
Songklanakarin Journal of Science and Technology , 2007,
Abstract: We conducted a phase I trial of Gynostemma pentaphyllum, grown in northern Thailand, to evaluate its safety in three groups of healthy volunteers. Fourteen, fifteen and fourteen volunteers respectively received the water extract of G. pentaphyllum in capsules at the doses of 50, 200 and 400 mg twice daily for two months. There were no major adverse events reported from any of the three groups throughout the study. Significant changes in hematological parameters, natural killer cell activities and the numbers of CD3+, CD4+ and CD8+ cells were not seen during taking the extract. Some biochemical parameters were significantly different from baseline data. Those values were, however, within normal limits and did not result in clinically significant conditions. Our results suggested that the water extract of G. pentaphyllum at the doses of 50, 200 or 400 mg twice daily given to healthy volunteers for two months was safe.
Safety of the aqueous extract of Portulaca grandiflora Hook in healthy volunteers
Pranee Chavalittumrong,Busarawan Sriwanthana,Archawin Rojanawiwat,Rungrueng Kijphati
Songklanakarin Journal of Science and Technology , 2007,
Abstract: A phase 1 clinical trial was performed in 16 healthy volunteers to primarily investigate safety of Portulaca grandiflora (P. grandiflora) as well as to preliminarily assess its efficacy on the immune system. The volunteers received 500 mg/day of P. grandiflora aqueous extract (250 mg capsule twice daily) for 2 months. No major side effects were reported from any of the subjects throughout the study. It was found that some significant changes in biochemical parameters were within normal limits. Hematological and immunological parameters were not altered after oral administration of P. grandiflora. Our results indicated that the P. grandiflora aqueous extract at the dose of 500 mg/day given to normal volunteers for 2 months was safe.
In vitro effects of Thai medicinal plants on human lymphocyte activity
Busarawan Sriwanthana,Weena Treesangsri,Bongkod Boriboontrakul,Somchit Niumsakul
Songklanakarin Journal of Science and Technology , 2007,
Abstract: We assessed the effects of Cleistocalyx nervosum var paniala, Gynostemma pentaphyllum, Gynura procumbens, Houttuynia cordata, Hyptis suaveolens, Portulaca grandiflora, Phytolacca americana and Tradescantia spathacea on lymphocyte proliferation and the effects of C. nervosum, G. pentaphyllum, H. suaveolens and P. grandiflora on natural killer (NK) cells activity. All of the extracts significantly stimulated human lymphocyte proliferative responses at various concentrations depending on each extract. The extracts of C. nervosum and H. suaveolens were significantly enhanced NK cells activity while those of G. pentaphyllum and P. grandiflora did not alter NK cells function. Our results suggested that the extracts of those plants have stimulating activity on human lymphocytes and could be clinically useful for modulating immune functions of the body.
Development of a Novel In Silico Docking Simulation Model for the Fine HIV-1 Cytotoxic T Lymphocyte Epitope Mapping
Masahiko Mori, Kei Matsuki, Tomoyuki Maekawa, Mari Tanaka, Busarawan Sriwanthana, Masaru Yokoyama, Koya Ariyoshi
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041703
Abstract: Introduction Class I HLA's polymorphism has hampered CTL epitope mapping with laborious experiments. Objectives are 1) to evaluate the novel in silico model in predicting previously reported epitopes in comparison with existing program, and 2) to apply the model to predict optimal epitopes with HLA using experimental results. Materials and Methods We have developed a novel in silico epitope prediction method, based on HLA crystal structure and a peptide docking simulation model, calculating the peptide-HLA binding affinity at four amino acid residues in each terminal. It was applied to predict 52 HIV best–defined CTL epitopes from 15-mer overlapping peptides, and its predictive ability was compared with the HLA binding motif-based program of HLArestrictor. It was then used to predict HIV-1 Gag optimal epitopes from previous ELISpot results. Results 43/52 (82.7%) epitopes were detected by the novel model, whereas 37 (71.2%) by HLArestrictor. We also found a significant reduction in epitope detection rates for longer epitopes in HLArestrictor (p = 0.027), but not in the novel model. Improved epitope prediction was also found by introducing both models, especially in specificity (p<0.001). Eight peptides were predicted as novel, immunodominant epitopes in both models. Discussion This novel model can predict optimal CTL epitopes, which were not detected by an existing program. This model is potentially useful not only for narrowing down optimal epitopes, but predicting rare HLA alleles with less information. By introducing different principal models, epitope prediction will be more precise.
The Effect of HLA Polymorphisms on the Recognition of Gag Epitopes in HIV-1 CRF01_AE Infection
Busarawan Sriwanthana, Masahiko Mori, Mari Tanaka, Sei Nishimura, Toshiyuki Miura, Panita Pathipvanich, Pathom Sawanpanyalert, Koya Ariyoshi
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041696
Abstract: Introduction The design of a globally effective vaccine rests on the identification of epitopes capable of eliciting effective cytotoxic T lymphocyte (CTL) responses across multiple HIV clades in different populations. This study aims to discern the effect of HLA polymorphisms and the cross-clade reactivity or clade-specificity of epitopes in Thailand where HIV-1 CRF01_AE is circulating. Materials and Methods 14 peptides based on consensus HIV-1 CRF01_AE amino acid sequences were designed for use in IFN-γ ELISpot assays and 51Cr release assays among 66 HIV-1 CRF01_AE-infected Thai patients. For ELISpot responders carrying HLA alleles currently unknown to restrict CRF01_AE epitopes, in silico epitope-HLA prediction was performed. Results 29/66 (43.9%) patients recognized at least one peptide. In total 79 responses were seen against all 14 peptides. 28/79 (35.4%) of the responses were in patients with HLA alleles previously reported to restrict CRF01_AE epitopes, 24/79 (30.4%) responses were in individuals with HLA alleles previously reported to restrict epitopes of HIV clades other than CRF01_AE, and the remaining 27/79 (34.2%) responses were not associated with HLA alleles previously known to restrict HIV epitopes. In silico epitope prediction detected 19 novel, epitope-HLA combinations, and 11/19 (57.9%) were associated with HLA-C alleles. We further confirmed a novel HLA restriction of a previously identified HIV-1 Gag epitope [p24122–130: PPIPVGDIY (PY9)] by HLA-B*40:01 with a standard 51Cr release assay. Discussion CTL recognition sites in HIV-1 Gag were similar among different clades but the HLA restriction differed in Thai patients. This disparity in HLA restriction along different populations illustrated the importance of clade- and population-specific HLA analysis prior to CTL vaccine design.
Unique CRF01_AE Gag CTL Epitopes Associated with Lower HIV-Viral Load and Delayed Disease Progression in a Cohort of HIV-Infected Thais
Masahiko Mori, Busarawan Sriwanthana, Nuanjun Wichukchinda, Chetsada Boonthimat, Naho Tsuchiya, Toshiyuki Miura, Panita Pathipvanich, Koya Ariyoshi, Pathom Sawanpanyalert
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0022680
Abstract: Cytotoxic T Lymphocytes (CTLs) play a central role in controlling HIV-replication. Although numerous CTL epitopes have been described, most are in subtype B or C infection. Little is known about CTL responses in CRF01_AE infection. Gag CTL responses were investigated in a cohort of 137 treatment-na?ve HIV-1 infected Thai patients with high CD4+ T cell counts, using gIFN Enzyme-Linked Immunospot (ELISpot) assays with 15-mer overlapping peptides (OLPs) derived from locally dominant CRF01_AE Gag sequences. 44 OLPs were recognized in 112 (81.8%) individuals. Both the breadth and magnitude of the CTL response, particularly against the p24 region, positively correlated with CD4+ T cell count and inversely correlated with HIV viral load. The breadth of OLP response was also associated with slower progression to antiretroviral therapy initiation. Statistical analysis and single peptide ELISpot assay identified at least 17 significant associations between reactive OLP and HLA in 12 OLP regions; 6 OLP-HLA associations (35.3%) were not compatible with previously reported CTL epitopes, suggesting that these contained new CTL Gag epitopes. A substantial proportion of CTL epitopes in CRF01_AE infection differ from subtype B or C. However, the pattern of protective CTL responses is similar; Gag CTL responses, particularly against p24, control viral replication and slow clinical progression.
HLA-Associated Immune Pressure on Gag Protein in CRF01_AE-Infected Individuals and Its Association with Plasma Viral Load
Goragoch Gesprasert,Nuanjun Wichukchinda,Masahiko Mori,Teiichiro Shiino,Wattana Auwanit,Busarawan Sriwanthana,Panita Pathipvanich,Pathom Sawanpanyalert,Toshiyuki Miura,Prasert Auewarakul,Arunee Thitithanyanont,Koya Ariyoshi
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0011179
Abstract: The human leukocyte antigen (HLA)-restricted cytotoxic T-lymphocyte (CTL) immune response is one of the major factors determining the genetic diversity of human immunodeficiency virus (HIV). There are few population-based analyses of the amino acid variations associated with the host HLA type and their clinical relevance for the Asian population. Here, we identified HLA-associated polymorphisms in the HIV-1 CRF01_AE Gag protein in infected married couples, and examined the consequences of these HLA-selected mutations after transmission to HLA-unmatched recipients.
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