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Search Results: 1 - 10 of 7333 matches for " Bucio Martha Irene "
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Comportamiento biológico de Triatoma pallidipennis (Hemiptera: Reduviidae) en el estado de Morelos, México Biological behavior of Triatoma pallidipennis (Hemiptera: Reduviidae) in the State of Morelos, México
Norma Bautista,Gloria Rojas,Irene De Haro,Martha Bucio
Boletín chileno de parasitología , 2001,
Abstract: T. pallidipennis was found naturally infected in localities of domestic, peridomestic and wild areas in the State of Morelos, México. In agreement with the triatomines high potentiality like vectors of T. cruzi, protozoan that causes Chagas' disease and the knowledge of these bugs in México, the aim of the present research was to collect triatomines for assessment of T. cruzi infection, to characterize and compare life cycles, identification of feeding preferences and observe indexes of experimental infectivity. Seven isolates of T. cruzi were obtained, from differents localities which curves of parasites showed different behaviors. Histopathological study, carried out by counting of amastigotes nests, which affected the gastrocnemius muscle and heard was observed. The life cycles of T. pallidipennis was completed in 131 days. The feeding preferences were rats, cats and pigs.
Comportamiento biológico de Triatoma pallidipennis (Hemiptera: Reduviidae) en el estado de Morelos, México
Bautista,Norma; Rojas,Gloria; De Haro,Irene; Bucio,Martha; Salazar Schettino,Paz María;
Boletín chileno de parasitología , 2001, DOI: 10.4067/S0365-94022001000200004
Abstract: t. pallidipennis was found naturally infected in localities of domestic, peridomestic and wild areas in the state of morelos, méxico. in agreement with the triatomines high potentiality like vectors of t. cruzi, protozoan that causes chagas' disease and the knowledge of these bugs in méxico, the aim of the present research was to collect triatomines for assessment of t. cruzi infection, to characterize and compare life cycles, identification of feeding preferences and observe indexes of experimental infectivity. seven isolates of t. cruzi were obtained, from differents localities which curves of parasites showed different behaviors. histopathological study, carried out by counting of amastigotes nests, which affected the gastrocnemius muscle and heard was observed. the life cycles of t. pallidipennis was completed in 131 days. the feeding preferences were rats, cats and pigs.
First Case of Natural Infection in Pigs: Review of Trypanosoma cruzi Reservoirs in Mexico
Salazar-Schettino Paz María,Bucio Martha Irene,Cabrera Margarita,Bautista Jacobo
Memórias do Instituto Oswaldo Cruz , 1997,
Abstract: An epidemiological research project was performed in the State of Morelos including collection of samples for blood smears and culture, serological tests, and xenodiagnoses from a total of 76 domestic and peridomestic mammals. Two strains of Trypanosoma cruzi were isolated by haemocultures; one from a pig (Sus scrofa), the first case of natural infection reported in Mexico, and the other from a dog (Canis familiaris). This study summarizes current information in Mexico concerning confirmed reservoirs of T. cruzi
Efecto de la temperatura y humedad relativa en la germinación de esporangios de Bremia lactucae Regel Effect of temperature and relative humidity on the germination of Bremia lactucae Regel sporangia
Ricardo Yá?ez López,Juan ángel Quijano Carranza,Carlos Manuel Bucio Villalobos,María Irene Hernández Zul
Revista mexicana de ciencias agrícolas , 2012,
Abstract: El propósito de este trabajo realizado en 2009, fue estudiar el efecto de la temperatura, y la humedad relativa, en la germinación de esporangios de Bremia lactucae Regel. El efecto de estas variables sobre la germinación de esporangios in vitro, se evaluó en cámaras de incubación mediante 21 tratamientos formados por los niveles de temperatura de 5, 10, 15, 20, 25, 30, 35 oC en combinación con humedades de 70, 80, 90%. La germinación se evaluó a las 24 h de establecer el tratamiento. Se realizó un análisis estadístico para determinar la importancia de estas variables y su interacción sobre la germinación de esporangios e inferir sobre el rango óptimo de temperatura y humedad. Se encontró que existe efecto significativo de la interacción entre estas variables sobre la germinación, presentándose esta desde los 5 oC hasta los 25 oC. El tratamiento que presentó el mayor porcentaje de germinación fue el de 10 oC en combinación con humedad relativa 90%. Con base en una análisis de superficie de respuesta. Se concluyó que el óptimo de germinación de esporangios se consigue con temperaturas de entre 9.5 y 12.5 oC con una humedad de 90%. The purpose of this work, performed in 2009, was to study the effect of temperature and relative humidity on the germination of Bremia lactucae Regel sporangia. The effect of these variables on the in vitro germination of sporangia was evaluated in incubation chambers, with 21 treatments formed by the temperature levels of 5, 10, 15, 20, 25, 30, 35 oC combined with humidities of 70, 80, 90%. Germination was evaluated 24 h after the treatment was established. A statistical analysis was carried out to determine the importance of these variables and their interaction on the germination of sporangia and infer on the optimum temperature and humidity ranges. We found that there is a significant effect of the interaction between these variables on germination, which appears starting at 5 oC until 25 oC. The treatment that presented the highest percentage of germination was 10 oC, in combination with a relative humidity of 90%. Based on an surface response analysis, we concluded that the optimum of sporangia germination can be obtained with with temperatures ranging from 9.5 to 12.5 oC with a relative humidity of 90%.
Seroprevalencia de anticuerpos contra Trypanosoma cruzi y su asociación con factores de riesgo en menores de 18 a?os de Veracruz, México
Salazar,Paz María; Rojas,Gloria; Bucio,Martha; Cabrera,Margarita; García,Guadalupe; Ruiz,Adela; Guevara,Yolanda; Tapia,Roberto;
Revista Panamericana de Salud Pública , 2007, DOI: 10.1590/S1020-49892007000700001
Abstract: objectives: to determine the prevalence of trypanasoma cruzi antibodies among the population under 18 years of age in the state of veracruz, mexico, and to identify risk factors associated with housing conditions and vector presence and distribution. methods: a cross-sectional, epidemiological study of the population under 18 years of age living in 10 of the 11 health districts of the state of veracruz, mexico, from 2000 to2001. presence of t. cruzi antibodies was determined by hemagglutination inhibition test (hai) and enzyme-linked immunosorbent assays (elisa) of serum samples spotted on filter paper, and confirmed by hai, elisa, and indirect immunofluorescence assay (ifa) on serum samples. a questionnaire was administered to determined housing conditions, and entomological indices for triatomines were calculated for both the intra- and peridomiciliary areas. the results were analyzed using bifactorial and multifactorial unconditional logistic regression. results: of the 150 individuals presenting an initial reaction, 14 were confirmed positive (5 by hai, elisa and ifa; 6 by hai and elisa, and 3 by elisa and ifa), for a total prevalence rate of 0.91% (95% ci: 0.85-0.94). all of the confirmed-positive cases were from five of 14 health districts, with the tuxpan district having the highest prevalence rate: 5.2% (95%ci: 1.2-9.0). risk factors included having noticed cracks in the house walls and gaps in the roof. the only species of vector captured was triatoma dimidiata. the entomologic indices for infestation, colonization, and natural infection were: 10.9%, 50.0%, and 9.0%, respectively. conclusions: active, vector-borne transmission of t. cruzi was confirmed in the state of veracruz, mexico, with an overall antibody seroprevalence rate of 0.19% among the population under 18 years of age. seroprevalence rates among this segment of the population, which serves as a sentinel group, should be closely monitored to determine if more stringent methods of vector control
Insecticide and community interventions to control Triatoma dimidiata in localities of the State of Veracruz, Mexico
Wastavino, Gloria Rojas;Cabrera-Bravo, Margarita;García De La Torre, Guadalupe;Vences-Blanco, Mauro;Ruiz Hernández, Adela;Bucio Torres, Martha;Guevara Gómez, Yolanda;Mesa, Alejandro;Salazar Schettino, Paz María;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000400015
Abstract: three different interventions to control triatoma dimidiata in the state of veracruz were implemented: x-1 = whole dwelling spraying, x-2 = middle wall spraying, x-3 = household cleaning. cyfluthrin was sprayed 3 times with 8 month intervals. after each spraying, insects were collected and sent to the laboratory to be recorded and to determine genus and species of the adult triatomine bugs, and nymphs were counted. trypanosoma cruzi presence was determined. with x-1, the infestation, colonization, and natural infection indexes were reduced to 0% in the 3 localities, with respect to t0. with x-2, the infestation index was reduced to 10% at t3 in 3 localities; the colonization index was reduced to 0% in only 1 locality at t3, and the natural infection index was reduced to 0% at t3. with x-3 the 3 indexes were not effectively reduced but they decreased with respect to the baseline study. insecticide application to the whole dwelling is a more efficient intervention than its application to only the lower half of the walls and to the cleaning of houses.
Evaluación de la función y sincronía de la contracción ventricular en pacientes con enfermedad de Chagas en estadio de latencia
Nivardo Sobrino, Ayax;Jiménez-ángeles, Luis;Bialostozky, David;Vázquez, Clara;Martínez, Irma;Salazar-Schettino, Paz Maria;Bucio-Torres, Martha;Ruiz-Hernández, Adela;Cabrera-Bravo, Margarita;
Archivos de cardiología de México , 2009,
Abstract: objective: to compare the left ventricular function and the ventricular synchrony in patients with chagas disease in latency stage respect to a control group. methods: we analyze a prospective, comparative, transversal and non randomized study of the left ventricular function (lvf) and the ventricular contraction synchronicity (vcs) in 36 subjects with positive serology for chagas disease (18 males and 18 females), with mean of 15 ± 5 years old. the findings were compared with respect to 23 control volunteers (11 males and 12 females) with mean of 28 ± 5 years old. lvf and vcs were evaluated using equilibrium radionuclide angiography images (erna). the comparison of both chagas and control populations was carried out by t student test for independent samples, considering a statistically significant value of p < 0.05. results: the parameters of the ventricular function and the ventricular synchronicity in subjects with positive serology for chagas disease were not statistically different with respect to the parameters of the control group. however, although they have a homogeneous contraction, the mean time of contraction for the right and the left ventricle is statistically smaller with respect to the control group. conclusions: in clinically incipient stages of chagas disease we do not found abnormalities in the ventricular function and the ventricular synchronicity. it's necessary to consider the follow up of the studied populations using indices for the identification of abnormalities of the autonomic nervous system.
Diagnóstico del virus rábico en quirópteros Rabies virus diagnosis in bats
Dámasa Irene López Santa Cruz,Jusayma González Arrebato,Martha Morales Leslie
Revista Cubana de Medicina Tropical , 2013,
Abstract: Introducción: los quirópteros son un importante reservorio y vector del virus rábico en el mundo. La enfermedad invariablemente lleva a la muerte, pero puede ser prevenida mediante la vacunación. Objetivo: detectar virus rábico a través de la técnica de inmunofluorescencia directa en quirópteros. Métodos: en el Centro Provincial de Higiene Epidemiología y Microbiología de la Provincia La Habana en el período de 2004 a 2009, se recibieron 651 muestras de cerebros de quirópteros para detectar el virus de la rabia. Se analizaron empleando el método de inmunofluorescencia directa. Resultados: la presencia del virus se confirmó en 5 muestras de cerebro de murciélagos. Se obtuvo 0,76 % de positividad. Conclusión: se demuestra que en Cuba circula el virus de la rabia en murciélagos y que aunque el porcentaje de positividad es bajo, se deben tomar las precauciones establecidas cuando se observen cambios de conducta en esta especie. Introduction: the bats are an important reservoir and vector of rabies virus in the world. The infection invariably leads to death, but can be prevented with vaccination. Objective: to detect rabies virus through direct immunofluorescence in bats. Method: the provincial Center of Hygiene, Epidemiology and Microbiology of Havana received 651 bat brain samples from 2004 to 2009 to test for rabies virus, using the direct immunofluorescence method. Results: the presence of virus was confirmed in 5 bat brain samples. Positivity rate was 0.76 %. Conclusions: it was demonstrated that rabies virus circulates in Cuba in bats and that although the positivity rate is low, so the established precautions should be taken when behavioral changes are observed in this species.
Análise da contamina o por Salmonella em ovos do tipo colonial através da rea o em cadeia da polimerase
Fl?res Maristela Lovato,Nascimento Vladimir Pinheiro do,Kader Ivonyr Irene Tróglio Abdel,Cardoso Martha
Ciência Rural , 2003,
Abstract: A identifica o de poedeiras comerciais infectadas por salmonelas tem sido um dos pontos fortes da profilaxia e conseqüente redu o de surtos de salmonelose em humanos associados ao consumo de ovos, sendo que a análise dos ovos pode ser mais um dos pontos de detec o da infec o, que, muitas vezes, cursa sem sinais clínicos. A Rea o em Cadeia da Polimerase (PCR) parece ser uma estratégia útil para detec o de Salmonella, pois vários autores têm utilizado a PCR para verificar a presen a da bactéria em carnes, fezes, tecidos, sangue, leite e ovos, com diferentes metodologias de manipula o das amostras. Foram analisados 360 ovos, procedentes de dez propriedades rurais, produtoras de ovos tipo colonial, no distrito de Camobi, em Santa Maria - RS. Os ovos foram divididos em grupos de seis, totalizando sessenta amostras. O exame bacteriológico foi realizado conforme metodologia preconizada pelas normas técnicas e a metodologia de extra o de DNA pelo fenol-clorofórmio. A PCR foi realizada para a amplifica o de um fragmento de DNA de 284 pb. A análise dos resultados n o demonstrou diferen a significativa entre a PCR e o bacteriológico. Todas as amostras positivas ao bacteriológico foram positivas na PCR, sendo que essa última detectou duas amostras a mais, devido a sua alta sensibilidade e especificidade, especialmente quando é sabido que os ovos apresentam uma popula o microbiana mista que, muitas vezes, impede o isolamento adequado das salmonelas no bacteriológico pela competi o com a flora bacteriana normalmente presente.
Risk Factors for Soil-Transmitted Helminth Infections during the First 3 Years of Life in the Tropics; Findings from a Birth Cohort
Stefanie K. Menzies,Alejandro Rodriguez,Martha Chico,Carlos Sandoval,Nely Broncano,Irene Guadalupe,Philip J. Cooper
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002718
Abstract: Background Soil-transmitted helminths (STH) infect more than 2 billion humans worldwide, causing significant morbidity in children. There are few data on the epidemiology and risk factors for infection in pre-school children. To investigate risk factors for infection in early childhood, we analysed data prospectively collected in the ECUAVIDA birth cohort in Ecuador. Methods and Findings Children were recruited at birth and followed up to 3 years of age with periodic collection of stool samples that were examined microscopically for STH parasites. Data on social, demographic, and environmental risk factors were collected from the mother at time of enrolment. Associations between exposures and detection of STH infections were analysed by multivariable logistic regression. Data were analysed from 1,697 children for whom a stool sample was obtained at 3 years. 42.3% had at least one STH infection in the first 3 years of life and the most common infections were caused by A. lumbricoides (33.2% of children) and T. trichiura (21.2%). Hookworm infection was detected in 0.9% of children. Risk of STH infection was associated with factors indicative of poverty in our study population such as Afro-Ecuadorian ethnicity and low maternal educational level. Maternal STH infections during pregnancy were strong risk factors for any childhood STH infection, infections with either A. lumbricoides or T. trichiura, and early age of first STH infection. Children of mothers with moderate to high infections intensities with A. lumbricoides were most at risk. Conclusions Our data show high rates of infection with STH parasites during the first 3 years of life in an Ecuadorian birth cohort, an observation that was strongly associated with maternal STH infections during pregnancy. The targeted treatment of women of childbearing age, in particular before pregnancy, with anthelmintic drugs could offer a novel approach to the prevention of STH infections in pre-school children.
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