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Search Results: 1 - 10 of 8174 matches for " Bruno Passet "
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Ubiquitous Gasp1 overexpression in mice leads mainly to a hypermuscular phenotype
Monestier Olivier,Brun Caroline,Heu Katy,Passet Bruno
BMC Genomics , 2012, DOI: 10.1186/1471-2164-13-541
Abstract: Background Myostatin, a member of the TGFβ superfamily, is well known as a potent and specific negative regulator of muscle growth. Targeting the myostatin signalling pathway may offer promising therapeutic strategies for the treatment of muscle-wasting disorders. In the last decade, various myostatin-binding proteins have been identified to be able to inhibit myostatin activity. One of these is GASP1 (Growth and Differentiation Factor-Associated Serum Protein-1), a protein containing a follistatin domain as well as multiple domains associated with protease inhibitors. Despite in vitro data, remarkably little is known about in vivo functions of Gasp1. To further address the role of GASP1 during mouse development and in adulthood, we generated a gain-of-function transgenic mouse model that overexpresses Gasp1 under transcriptional control of the human cytomegalovirus immediate-early promoter/enhancer. Results Overexpression of Gasp1 led to an increase in muscle mass observed not before day 15 of postnatal life. The surGasp1 transgenic mice did not display any other gross abnormality. Histological and morphometric analysis of surGasp1 rectus femoris muscles revealed an increase in myofiber size without a corresponding increase in myofiber number. Fiber-type distribution was unaltered. Interestingly, we do not detect a change in total fat mass and lean mass. These results differ from those for myostatin knockout mice, transgenic mice overexpressing the myostatin propeptide or follistatin which exhibit both muscle hypertrophy and hyperplasia, and show minimal fat deposition. Conclusions Altogether, our data give new insight into the in vivo functions of Gasp1. As an extracellular regulatory factor in the myostatin signalling pathway, additional studies on GASP1 and its homolog GASP2 are required to elucidate the crosstalk between the different intrinsic inhibitors of the myostatin.
Fidgetin-Like1 Is a Strong Candidate for a Dynamic Impairment of Male Meiosis Leading to Reduced Testis Weight in Mice
David L'H?te,Magalie Vatin,Jana Auer,Johan Castille,Bruno Passet,Xavier Montagutelli,Catherine Serres,Daniel Vaiman
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0027582
Abstract: In a previous work, using an interspecific recombinant congenic mouse model, we reported a genomic region of 23 Mb on mouse chromosome 11 implicated in testis weight decrease and moderate teratozoospermia (~20–30%), a Quantitative Trait Locus (QTL) called Ltw1. The objective of the present study is to identify the gene underlying this phenotype.
Transcriptomic Analysis Brings New Insight into the Biological Role of the Prion Protein during Mouse Embryogenesis
Manal Khalifé, Rachel Young, Bruno Passet, Sophie Halliez, Marthe Vilotte, Florence Jaffrezic, Sylvain Marthey, Vincent Béringue, Daniel Vaiman, Fabienne Le Provost, Hubert Laude, Jean-Luc Vilotte
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0023253
Abstract: The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.
Overexpression of miR-30b in the Developing Mouse Mammary Gland Causes a Lactation Defect and Delays Involution
Sandrine Le Guillou, Nezha Sdassi, Johann Laubier, Bruno Passet, Marthe Vilotte, Johan Castille, Denis Lalo?, Jacqueline Polyte, Stéphan Bouet, Florence Jaffrézic, Edmond-Paul Cribiu, Jean-Luc Vilotte, Fabienne Le Provost
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045727
Abstract: Background MicroRNA (miRNA) are negative regulators of gene expression, capable of exerting pronounced influences upon the translation and stability of mRNA. They are potential regulators of normal mammary gland development and of the maintenance of mammary epithelial progenitor cells. This study was undertaken to determine the role of miR-30b on the establishment of a functional mouse mammary gland. miR-30b is a member of the miR-30 family, composed of 6 miRNA that are highly conserved in vertebrates. It has been suggested to play a role in the differentiation of several cell types. Methodology/Principal Findings The expression of miR-30b was found to be regulated during mammary gland development. Transgenic mice overexpressing miR-30b in mammary epithelial cells were used to investigate its role. During lactation, mammary histological analysis of the transgenic mice showed a reduction in the size of alveolar lumen, a defect of the lipid droplets and a growth defect of pups fed by transgenic females. Moreover some mammary epithelial differentiated structures persisted during involution, suggesting a delay in the process. The genes whose expression was affected by the overexpression of miR-30b were characterized by microarray analysis. Conclusion/Significance Our data suggests that miR-30b is important for the biology of the mammary gland and demonstrates that the deregulation of only one miRNA could affect lactation and involution.
Prion Protein and Shadoo Are Involved in Overlapping Embryonic Pathways and Trophoblastic Development
Bruno Passet,Rachel Young,Samira Makhzami,Marthe Vilotte,Florence Jaffrezic,Sophie Halliez,Stéphan Bouet,Sylvain Marthey,Manal Khalifé,Colette Kanellopoulos-Langevin,Vincent Béringue,Fabienne Le Provost,Hubert Laude,Jean-Luc Vilotte
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041959
Abstract: The potential requirement of either the Prion or Shadoo protein for early mouse embryogenesis was recently suggested. However, the current data did not allow to precise the developmental process that was affected in the absence of both proteins and that led to the observed early lethal phenotype. In the present study, using various Prnp transgenic mouse lines and lentiviral vectors expressing shRNAs that target the Shadoo-encoding mRNA, we further demonstrate the specific requirement of at least one of these two PrP-related proteins at early developmental stages. Histological analysis reveals developmental defect of the ectoplacental cone and important hemorrhage surrounding the Prnp-knockout-Sprn-knockdown E7.5 embryos. By restricting the RNA interference to the trophoblastic cell lineages, the observed lethal phenotype could be attributed to the sole role of these proteins in this trophectoderm-derived compartment. RNAseq analysis performed on early embryos of various Prnp and Sprn genotypes indicated that the simultaneous down-regulation of these two proteins affects cell-adhesion and inflammatory pathways as well as the expression of ectoplacental-specific genes. Overall, our data provide biological clues in favor of a crucial and complementary embryonic role of the prion protein family in Eutherians and emphasizes the need to further evaluate its implication in normal and pathological human placenta biology.
Brain transcriptional stability upon prion protein-encoding gene invalidation in zygotic or adult mouse
Sead Chadi, Rachel Young, Sandrine Le Guillou, Ga?lle Tilly, Frédérique Bitton, Marie-Laure Martin-Magniette, Ludivine Soubigou-Taconnat, Sandrine Balzergue, Marthe Vilotte, Coralie Peyre, Bruno Passet, Vincent Béringue, Jean-Pierre Renou, Fabienne Le Provost, Hubert Laude, Jean-Luc Vilotte
BMC Genomics , 2010, DOI: 10.1186/1471-2164-11-448
Abstract: Only subtle transcriptomic differences resulting from the Prnp knockout could be evidenced, beside Prnp itself, in the analyzed adult brains following microarray analysis of 24 109 mouse genes and QPCR assessment of some of the putatively marginally modulated loci. When performed at the adult stage, neuronal Prnp disruption appeared to sequentially induce a response to an oxidative stress and a remodeling of the nervous system. However, these events involved only a limited number of genes, expression levels of which were only slightly modified and not always confirmed by RT-qPCR. If not, the qPCR obtained data suggested even less pronounced differences.These results suggest that the physiological function of PrP is redundant at the adult stage or important for only a small subset of the brain cell population under classical breeding conditions. Following its early reported embryonic developmental regulation, this lack of response could also imply that PrP has a more detrimental role during mouse embryogenesis and that potential transient compensatory mechanisms have to be searched for at the time this locus becomes transcriptionally activated.The pivotal role that the prion protein (PrP) plays in transmissible spongiform encephalopathies (TSE) is now well established [[1,2] for recent reviews]. The conversion of this host-encoded protein to an abnormal, partially proteinase K resistant, isoform is a hallmark of most TSEs and PrP is the only known constituent of mammalian prions [3]. The Prnp gene that encodes for PrP, is expressed in a broad range of vertebrate tissues but most abundantly in the central nervous system [4].Although PrP is evolutionary conserved, suggesting that it has an important role, its physiological function remains unclear even though its implication in neuroprotection, response to oxidative stress, cell proliferation and differentiation, synaptic function and signal transduction has been proposed [5,6]. Its temporal regulation led also to susp
The Population Structure of Acinetobacter baumannii: Expanding Multiresistant Clones from an Ancestral Susceptible Genetic Pool
Laure Diancourt,Virginie Passet,Alexandr Nemec,Lenie Dijkshoorn,Sylvain Brisse
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0010034
Abstract: Outbreaks of hospital infections caused by multidrug resistant Acinetobacter baumannii strains are of increasing concern worldwide. Although it has been reported that particular outbreak strains are geographically widespread, little is known about the diversity and phylogenetic relatedness of A. baumannii clonal groups. Sequencing of internal portions of seven housekeeping genes (total 2,976 nt) was performed in 154 A. baumannii strains covering the breadth of known diversity and including representatives of previously recognized international clones, and in 19 representatives of other Acinetobacter species. Restricted amounts of diversity and a star-like phylogeny reveal that A. baumannii is a genetically compact species that suffered a severe bottleneck in the recent past, possibly linked to a restricted ecological niche. A. baumannii is neatly demarcated from its closest relative (genomic species 13TU) and other Acinetobacter species. Multilocus sequence typing analysis demonstrated that the previously recognized international clones I to III correspond to three clonal complexes, each made of a central, predominant genotype and few single locus variants, a hallmark of recent clonal expansion. Whereas antimicrobial resistance was almost universal among isolates of these and a novel international clone (ST15), isolates of the other genotypes were mostly susceptible. This dichotomy indicates that antimicrobial resistance is a major selective advantage that drives the ongoing rapid clonal expansion of these highly problematic agents of nosocomial infections.
Harmonizing Budgeting and Accounting: The Case of Italy  [PDF]
Adriana Bruno
Open Journal of Accounting (OJAcct) , 2014, DOI: 10.4236/ojacct.2014.31005
Abstract: Many reforms have been undertaken in recent years; indeed, scholars have concentrated their attention on ac-counting techniques, financial management, and the potential for adding tools to support managerial reform agendas [1-3]. This paper extends prior research by focusing on the manner of diffusion ongoing reform process, through the adoption of the new version of Financial Management Reform process model [3]. The objective of this research is to investigate how a new accounting system has been introduced in the public sector in a processual and outcome-oriented analysis [4]. The study setting for this research is the Italian government, specifically the intermediate or regional level government, Campania Region, where a trial period is underway. The objective is to discover what obstacles and difficulties might exist when implementing a harmonized accounting and budgeting system while moving toward an accrual accounting system. The study reveals the main paradox in the implementation of the reform. This kind of study could add fresh knowledge to the literature and highlight common problems encountered when implementing accounting reforms. Additionally it could help set a standard to assist public entities in developing and implementing accounting guidelines.
A Few Reflections on the Reasons Why Cooperative Firms Have Failed to Gain a Firm Foothold  [PDF]
Bruno Jossa
Open Journal of Business and Management (OJBM) , 2015, DOI: 10.4236/ojbm.2015.33027
Abstract: After the inception of the cooperative movement in the mid-19th century, it was widely held that capitalistic businesses would soon be replaced by a system of worker-controlled firms and a wealth of policy proposals called for a major impulse to cooperation in later years also. But cooperation has not met with the hoped-for success. How do we account for this? To our day, employee management specialists have been unable to provide a satisfactory explanation for the fact that the policy proposal to introduce democratic firm control still carries little consensus despite the collapse of the Soviet model of communism. In this paper, we give some answers to the question and emphasise the idea that progress in the direction of a generalised system of cooperative firms would amount to a considerable improvement over capitalism, but will hardly be made without the effective contribution of intellectuals and political parties.
Competition in a Democratic Firm System: Failures and Constraints  [PDF]
Bruno Jossa
Modern Economy (ME) , 2017, DOI: 10.4236/me.2017.811092
Abstract: Considering that businesses face bankruptcy when their aggregate costs exceed their revenues, the cancellation of the largest production cost item—wages and salaries—in an employee-managed firm system is an effective safeguard against bankruptcy. For this and other reasons, the author argues that risks of insolvency are unlikely to scare democratic firms into accepting the capitalistic logic of cut-throat competition.
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