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Search Results: 1 - 10 of 212 matches for " Brondani "
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Health Technology Assessment Fireside: Antibiotic Prophylaxis and Dental Treatment in Canada
Mario A. Brondani
Journal of Pharmaceutics , 2013, DOI: 10.1155/2013/365635
Abstract: Objectives. This paper discusses the controversies surrounding the antibiotic prophylaxis preceding dental interventions within the following research question: how effective is dental antibiotic prophylaxis in preventing comorbidity and complications in those at risk? Methods. A synthesis of the available literature regarding antibiotic prophylaxis in dentistry was conducted under the lenses of Kazanjian’s framework for health technology assessment with a focus on economic concerns, population impact, social context, population at risk, and the effectiveness of the evidence to support its use. Results. The papers reviewed show that we have been using antibiotic prophylaxis without a clear and full understanding of its benefits. Although the first guideline for antibiotic prophylaxis was introduced in 1990, it has been revised on several occasions, from 1991 to 2011. Evidence-based clinical guidelines are yet to be seen. Conclusions. Any perceived potential benefit from administering antibiotic prophylaxis before dental procedures must be weighed against the known risks of lethal toxicity, allergy, and development, selection, and transmission of microbial resistance. The implications of guideline changes and lack of evidence for the full use of antibiotic prophylaxis for the teaching of dentistry have to be further discussed. 1. Introduction Antibiotic prophylaxis is understood as a preventive health measure to minimize harmful interaction between the oral bacteria in the bloodstream with matrix molecules and platelets at body sites (e.g., organs, tissues), which could lead to generalized septicemia [1]. “Because it is not possible to predict when a susceptible patient will develop an infection, prophylactic antibiotics are recommended when these patients undergo procedures that might produce bacteremia” [2]. A prophylactic antibiotic is then given prior to the dental procedure on the basis of its activity against oral bacteria, its toxicity, and its cost. This review paper discusses the controversies surrounding this prophylaxis as a health technology within the following research question: how effective is dental antibiotic prophylaxis in preventing comorbidity and complications in those at risk? In order to address this research question, a synthesis of the available literature on the issue under the lenses of Kazanjian’s framework for health technology assessment (HTA) [3, 4] is performed. 2. The Framework for Health Technology Assessment In 2004, Kazanjian presented a framework for health technological decisions with the five overlapping components
A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus
Rosana PV Brondani, Emlyn R Williams, Claudio Brondani, Dario Grattapaglia
BMC Plant Biology , 2006, DOI: 10.1186/1471-2229-6-20
Abstract: The consensus map covers ~90% of the recombining genome of Eucalyptus, involves 234 mapped EMBRA loci on 11 linkage groups, an observed length of 1,568 cM and a mean distance between markers of 8.4 cM. A compilation of all microsatellite linkage information published in Eucalyptus allowed us to establish the homology among linkage groups between this consensus map and other maps published for E. globulus. Comparative mapping analyses also resulted in the linkage group assignment of other 41 microsatellites derived from other Eucalyptus species as well as candidate genes and QTLs for wood and flowering traits published in the literature. This report significantly increases the availability of microsatellite markers and mapping information for species of Eucalyptus and corroborates the high conservation of microsatellite flanking sequences and locus ordering between species of the genus.This work represents an important step forward for Eucalyptus comparative genomics, opening stimulating perspectives for evolutionary studies and molecular breeding applications. The generalized use of an increasingly larger set of interspecific transferable markers and consensus mapping information, will allow faster and more detailed investigations of QTL synteny among species, validation of expression-QTL across variable genetic backgrounds and positioning of a growing number of candidate genes co-localized with QTLs, to be tested in association mapping experiments.Eucalypts are the most widely planted hardwood trees in the world occupying globally more than 18 million hectares [1]. While E. globulus is the premier species for temperate zones plantations in Portugal, Spain, Chile and Australia, elite hybrid clones involving E. grandis and E. urophylla are extensively used by the pulp and paper industry in tropical regions of Brazil, South Africa, India and Congo because of its wood quality, rapid growth, canker disease resistance and high volumetric yield [2].Genetic mapping became
Numerical experimental comparison of dam break flows with non-Newtonian fluids
Minussi, Roberta Brondani;Maciel, Geraldo de Freitas;
Journal of the Brazilian Society of Mechanical Sciences and Engineering , 2012, DOI: 10.1590/S1678-58782012000200008
Abstract: the dam-break flow involving non-newtonian fluids is a type of flow commonly observed in nature as well as in common industrial processes. experiments of non-newtonian dam-break flows were conducted in horizontal channels and aqueous solutions of carbopol 940 were used, which were modeled by the herschel-bulkley constitutive equation. their flows were filmed and the frames were compared with numerical simulations. two particular results were analyzed: the front wave evolution with time and its stop distance. the cfx software was employed and the simulations were conducted with the vof method. both results, numerical and experimental, were compared with shallow water approximation solutions found in literature. the numerical code, which uses complete momentum equations, showed better agreement with the experiments than those using shallow water equations. it seems that the hypotheses used by the shallow water approximated equations are not appropriate for the first instants of the flow, just after the dam-break and errors are introduced. probably, these errors are propagated producing the differences encountered.
A história infantil como recurso na compreens o do processo saúde-doen a pela crian a com HIV El cuento infantil como recurso en la comprensión del proceso salud-enfermedad por ni os con HIV A story for children to help children with HIV understand the health-disease process
Jeanine Porto Brondani,Eva Neri Rubim Pedro
Revista Gaúcha de Enfermagem , 2013,
Abstract: O estudo analisou como uma história infantil, contendo quest es relacionadas à Síndrome da Imunodeficiência Adquirida, contribui para a compreens o do processo saúde-doen a pela crian a com o Vírus da Imunodeficiência Humana. Foi realizado, em Porto Alegre, RS, entre maio e dezembro de 2011. Participaram cinco crian as, com idades entre sete e nove anos, e seus cuidadores. Utilizou-se grupo focal e entrevista, e os dados foram submetidos à análise temática de conteúdo. Emergiram duas categorias: Identifica o com a história e a rela o com o processo saúde-doen a e Compreens o da história e do processo saúde-doen a. Os resultados demonstraram que a história infantil é um recurso para conversar com as crian as sobre o processo saúde-doen a sem revelar o diagnóstico, levando a uma compreens o de si e do tratamento. Considera-se que esse recurso pode ser uma estratégia para auxiliar os cuidadores e profissionais da saúde a iniciar o processo de revela o do diagnóstico. El estudio analizó cómo el cuento infantil con cuestiones sobre el SIDA contribuye a la comprensión del proceso salud-enfermedad por el ni o con VIH. Fue realizado en clínica pediátrica de Porto Alegre/RS, de mayo a diciembre de 2011. Participaron cinco ni os entre siete y nueve a os y sus cuidadores. Los datos fueran recogidos en grupo focal y entrevista y analizados por medio del análisis temático de contenido. Emergieron dos categorías: La identificación con la historia y la relación con el proceso salud-enfermedad; y La comprensión de la historia y del proceso salud-enfermedad. Los resultados demostraron que el cuento infantil es un recurso para hablar con ni os sobre el proceso salud-enfermedad, sin revelar el diagnóstico, llevándolos a una comprensión de su situación y la importancia del tratamiento. Consideramos que ese recurso puede ser una estrategia para ayudar a cuidadores y a profesionales de salud para iniciar el proceso de revelación. This study analyzed how a story for children related to AIDS contributed to the understanding of the health-disease process of children with HIV. It was conducted at the Pediatric Clinic in Porto Alegre/RS from May to December 2011. The participants were five children aged between seven and nine years and their caregivers. The data were collected by a focal group through interviews and submitted to thematic content analysis. Two categories were found: identification with the story, relationship with the health-disease process, understanding of the story, and the health-disease process. The results demonstrated that stories for children are resour
Concentra es de ácido indolbutírico no enraizamento de Cryptomeria japonica
Dagma Kratz,Ivar Wendling,Gilvano Ebling Brondani
Journal of Biotechnology and Biodiversity , 2011,
Abstract: Cryptomeria japonica is a conifer of great load that detaches for the fast growth and good lumber potential. In spiteof could being propagated by seeds, the vegetative propagation of Cryptomeria japonica is justified for themaintenance of the desirable characteristics of selected plus trees. In function of this and the lack of informationabout vegetative propagation out of Japan, this work had as objective to verify the effect of indolbutiric acid (IBA)and alcohol on rooting of cuttings of three adult clones of Cryptomeria japonica. The cuttings from three trees withnine years old, after prepared, received phytosanitary treatment for the clear, followed by the treatment withdifferent concentrations of IBA (T1 = 0 mg L-1; T2 = water + alcohol (1:1 v/v); T3 = 2,000 mg L-1 IBA; T4 = 4,000mg L-1 IBA; T5 = 8,000 mg L-1 IBA). The best results showed that the studied clones presents variation in clones interms of the evaluated characteristics and has low level of rooting independent of the tested concentrations of IBA.the concentration with maximum technical efficiency is around 4,000 mg L-1 IBA, with 22.9% of rooting for clone 2.
Determination of genetic variability of traditional varieties of Brazilian rice using microsatellite markers
Brondani, Claudio;Borba, Tereza Cristina Oliveira;Rangel, Paulo Hideo Nakano;Brondani, Rosana Pereira Vianello;
Genetics and Molecular Biology , 2006, DOI: 10.1590/S1415-47572006000400017
Abstract: the rice (oryza sativa) breeding program of the rice and bean research center of the brazilian agricultural company empresa brasileira de pesquisa agropecuária (embrapa) is well established and provides new cultivars every year to attend the demand for improved high yielding varieties with tolerance to biotic and abiotic stresses. however, the elite genitors used to compose new populations for selection are closely related, contributing to the yield plateau reached in the last 20 years. to overcome this limit, it is necessary to broaden the genetic basis of the cultivars using diverse germplasm such as wild relatives or traditional varieties, with the latter being more practical because they are more easily crossed with elite germplasm to accelerate the recovery of modern plant types in the breeding lines. the objective of our study was to characterize the allelic diversity of 192 traditional varieties of brazilian rice using 12 simple sequence repeat (ssr or microsatellite) markers. the germplasm was divided into 39 groups by common name similarity. a total of 176 alleles were detected, 30 of which (from 23 accessions) were exclusive. the number of alleles per marker ranged from 6 to 22, with an average of 14.6 alleles per locus. we identified 16 accessions as a mixture of pure lines or heterozygous plants. dendrogram analysis identified six clusters of identical accessions with different common names and just one cluster with identical accessions with the same common name, indicating that ssr markers are fundamental to determining the genetic relationship between landraces. a subset of 24 landraces, representatives of the 13 similarity groups plus the 11 accessions not grouped, was the most variable set of genotypes analyzed. these accessions can be used as genitors to increase the genetic variability available to rice breeding programs.
Development of microsatellite markers for the genetic analysis of Magnaporthe grisea
Brondani, Claudio;Brondani, Rosana Pereira Vianello;Garrido, Lucas da Ressurrei??o;Ferreira, Márcio Elias;
Genetics and Molecular Biology , 2000, DOI: 10.1590/S1415-47572000000400009
Abstract: an ag microsatellite-enriched genomic dna library was constructed for magnaporthe grisea (anamorph pyricularia grisea), the causal agent of rice blast. seventy-two dna clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new pcr-based molecular markers to be used in genetic studies of the fungus. twenty-four of these clones were selected to design primer pairs for the pcr amplification of microsatellite alleles. single spore cultures of m. grisea isolated from rice and wheat in brazil, colombia and china were genotyped at three microsatellite loci. isolates from southern brazil were predominantly monomorphic at the tested ssr loci, indicating a low level of genetic variability in these samples. however, seven alleles were observed at the mgm-1 locus in isolates from central brazil and at least nine alleles were detected at the same locus in a sample of colombian isolates. polymorphism analysis at ssr loci is a simple and direct approach for estimating the genetic diversity of m. grisea isolates and a powerful tool for studying m. grisea genetics.
Genetic map of the common bean using a breeding population derived from the Mesoamerican gene pool
Luciana Gomes Ferreira,Gláucia Salle Cortopassi Buso,Rosana Pereira Vianello Brondani,Cláudio Brondani
Crop Breeding and Applied Biotechnology , 2010,
Abstract: The mapping population consisted of 94 F2 generation plants derived from a cross between the CNFC 7812and CNFC 8056 lines, with different protein contents, 24% and 19% respectively. Seven hundred and fifty-two molecularmarkers were tested among the parents and four individuals from the segregant population. A total of 101 loci were used todevelop the genetic map. The polymorphism rate was 8.3% and 23.2% for the microsatellite and RAPD markers, respectively.The sizes of the linkage groups ranged from 6.7 to 139.0 cM , presenting a mean of 49.4 ± 36.8. The map length was 840.7cM and the mean group length was 45.9 cM. The average distance between the framework loci was 16.1 cM. This map wascompared with international reference bean maps and results were discussed. The construction of the genetic map fromparents of the same center of origin and the commercial grain type were discussed.
Development of microsatellite markers for the genetic analysis of Magnaporthe grisea
Brondani Claudio,Brondani Rosana Pereira Vianello,Garrido Lucas da Ressurrei??o,Ferreira Márcio Elias
Genetics and Molecular Biology , 2000,
Abstract: An AG microsatellite-enriched genomic DNA library was constructed for Magnaporthe grisea (anamorph Pyricularia grisea), the causal agent of rice blast. Seventy-two DNA clones containing microsatellite repeats were isolated and sequenced in order to develop a series of new PCR-based molecular markers to be used in genetic studies of the fungus. Twenty-four of these clones were selected to design primer pairs for the PCR amplification of microsatellite alleles. Single spore cultures of M. grisea isolated from rice and wheat in Brazil, Colombia and China were genotyped at three microsatellite loci. Isolates from southern Brazil were predominantly monomorphic at the tested SSR loci, indicating a low level of genetic variability in these samples. However, seven alleles were observed at the MGM-1 locus in isolates from Central Brazil and at least nine alleles were detected at the same locus in a sample of Colombian isolates. Polymorphism analysis at SSR loci is a simple and direct approach for estimating the genetic diversity of M. grisea isolates and a powerful tool for studying M. grisea genetics.
Community-Based Research among Marginalized HIV Populations: Issues of Support, Resources, and Empowerment
Mario Brondani,Nardin R. Moniri,R. Paul Kerston
Interdisciplinary Perspectives on Infectious Diseases , 2012, DOI: 10.1155/2012/601027
Abstract: A research question was posed to us by a local HIV-resource organization interested in exploring the educational and service needs of those unreached. In order to properly address this inquiry, we developed a community-based participatory research by training peer-led volunteers to facilitate focus-group discussions within Aboriginal and refugees participants following an interview guide. We gathered Aboriginal people and refugees separated into three focus groups each, enrolling a total of 41 self-identified HIV-positive, 38 males. The discussions were tape recorded upon consent and lasted between 59 and 118 minutes. We analyzed the thematic information collected interactively through constant comparison. The qualitative data leading to categories, codes, and themes formed the basis for the spatial representation of a conceptual mapping. Both groups shared similar struggles in living with HIV and in properly accessing local nonmedical HIV resources and discussed their concerns towards the need for empowerment and support to take control of their health. 1. HIV Resource Services and Marginalized Communities The available literature has been quite prolific on issues around the medical side of HIV, particularly the uptake of HIV antiretroviral therapy adherence, cost, availability, and side effects [1–4]. Very few have discussed access and use of nonmedical HIV resources services [5]. Vancouver, Canada, has experienced a decrease in new HIV diagnoses [6], while new HIV cases amongst Aboriginal peoples and other populations continue to rise [7, 8]. New immigrants and refugees from HIV-endemic countries add to this pool when arriving to Canada [9, 10]. The strategic plan of the BC Center for Excellence in HIV/AIDS (BC-CfE) and reports from the BC Center for Disease Control have highlighted that different HIV-marginalized population groups in Vancouver remain at risk for further health struggles and isolation unmitigated by stigma and fear [5, 9]. Our understanding of marginalised communities encompass those who are disadvantaged in terms of the structures and conditions that shape their lives, health outcomes, and social positions in society [11]. In order to utilize the full advantage of HIV/AIDS information including printed media, support groups, and drop-ins, these marginalised groups must be aware of the existence of these resource services. However, there seems to be a gap between the resources that are being offered and those accessing them [2, 12]. Based on the current trend of HIV infection identified in the BCCDC and BC-CfE reports, different HIV
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