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Search Results: 1 - 10 of 53993 matches for " Brigitte David-Watine "
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Silencing Nuclear Pore Protein Tpr Elicits a Senescent-Like Phenotype in Cancer Cells
Brigitte David-Watine
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0022423
Abstract: Background Tpr is a large coiled-coil protein located in the nuclear basket of the nuclear pore complex for which many different functions were proposed from yeast to human. Methodology/Principal Findings Here we show that depletion of Tpr by RNA interference triggers G0–G1 arrest and ultimately induces a senescent-like phenotype dependent on the presence of p53. We also found that Tpr depletion impairs the NES [nuclear export sequence]-dependent nuclear export of proteins and causes partial co-depletion of Nup153. In addition Tpr depletion impacts on level and function of the SUMO-protease SENP2 thus affecting SUMOylation regulation at the nuclear pore and overall SUMOylation in the cell. Conclusions Our data for the first time provide evidence that a nuclear pore component plays a role in controlling cellular senescence. Our findings also point to new roles for Tpr in the regulation of SUMO-1 conjugation at the nuclear pore and directly confirm Tpr involvement in the nuclear export of NES-proteins.
Actin polymerisation at the cytoplasmic face of eukaryotic nuclei
Sylvia Münter, Jost Enninga, Rafael Vazquez-Martinez, Erwan Delbarre, Brigitte David-Watine, Ulf Nehrbass, Spencer L Shorte
BMC Cell Biology , 2006, DOI: 10.1186/1471-2121-7-23
Abstract: Using a fluorescent conjugate of the actin binding drug cytochalasin D (CD-BODIPY) we provide evidence that polymerising actin accumulates in vicinity to the NE. In addition, both transiently expressed fluorescent actin and cytoplasmic micro-injection of fluorescent actin resulted in accumulation of actin at the NE-membrane. Consistent with the idea that the cytoplasmic phase of NE-membranes can support this novel pool of perinuclear actin polymerisation we show that isolated, intact, differentiated primary hepatocyte nuclei support actin polymerisation in vitro. Further this phenomenon was inhibited by treatments hindering steric access to outer-nuclear-membrane proteins (e.g. wheat germ agglutinin, anti-nesprin and anti-nucleoporin antibodies).We conclude that actin polymerisation occurs around interphase nuclei of living cells at the cytoplasmic phase of NE-membranes.The nuclear envelope (NE) consists of two adjacent membranes, the inner (INM) and the outer nuclear membrane (ONM). The ONM is functionally connected to the endoplasmic-reticulum (ER) and contains numerous ribosomes, while the INM contains a unique set of transmembrane proteins and maintains close contact with chromatin in the nuclear matrix through the nuclear lamina network [1,2]. The ONM/ER comprising a single continuous membrane system is connected to the INM through nuclear pore complexes (NPC), which constitute the unique gateway for macromolecular transport across the nuclear-cytoplasmic boundary [3,4]. The cytoskeletal protein actin may play a role in NE function. This view has received support from recent evidence suggesting that the NE-membrane system is physically connected to the cytoplasmic microfilament network. For example, ONM proteins containing giant spectrin repeats or SUN (for Sad1p, UNC-84 homology) domains are involved in nuclear anchorage and migration, probably via actin filament interactions [5,6]. In metazoan cells, the integral ONM protein nesprin (member of the Syne/ANC-1
Further comments on Prognostic factors of small-cell lung cancer in Okayama Lung Cancer Study Group Trials. How about a more precise laboratory technique?
Watine J
Acta Medica Okayama , 1999,
Coyote Predation Effects on White-Tailed Deer Fawns  [PDF]
Lauren N. Watine, William M. Giuliano
Natural Resources (NR) , 2016, DOI: 10.4236/nr.2016.711050
Abstract: Coyotes (Canis latrans) are a relatively new predator to the southeastern United States, and may be negatively impacting white-tailed deer (Odocoileus virginianus; hereafter, deer) populations. Our objectives were to evaluate the impacts of coyotes on deer fawns by assessing deer fawn survival and cause-specific mortality, and gain an understanding of factors affecting fawn survival and coyote predation. We captured and radio collared 30 fawns in the Red Hills region of Florida and Georgia, USA (2012-2013). Fawns were monitored for 12 weeks for survival and cause-specific mortality, and we quantified habitat and environmental characteristics of birth sites. Predation (n = 19; 95%) was the leading cause of fawn mortality (n = 20; 67%), with coyote predation (n = 14; 74%) being the most important type of predation. Survival rates for all fawns were greater (P = 0.048) where coyotes were removed compared to non-removal sites, with 50% and 25% of fawns surviving to 12 weeks on coyote-removal and non-removal sites, respectively. Survival rates of fawns ultimately predated by coyotes were greater (P = 0.096) on coyote-removal than non-removal sites, with 40% and 50% of fawns predated by coyotes within 12 weeks on coyote-removal and non-removal sites, respectively. Survival of all fawns and those predated by coyotes was lower when fawns were born at sites with greater hardwood basal area, total basal area, and canopy closure; and survival improved if born in or near hardwood, natural pine, and managed (planted) pine cover types. Increased canopy cover within 10 m of the birth site was selected by adult females for birth sites of all fawns and those that were predated by coyotes. Compared with fawns that lived, all dying fawns and those predated by coyotes had less shrub cover within 5 m and less grass cover at and within 10 m of the birth site. Coyote removal increased fawn daily survival rates, and habitat played a role in fawn survival.
Factors Determining Coyote (Canis latrans) Diets  [PDF]
Lauren N. Watine, William M. Giuliano
Open Journal of Ecology (OJE) , 2017, DOI: 10.4236/oje.2017.713045
Abstract: Although studies have documented the potential for coyote (Canis latrans) food use to negatively affect wildlife populations and domesticated animals, they are often equivocal, possibly because most are of small spatial extent, and little is known of factors determining coyote diets. Our objectives were to quantify the diet and identify factors determining coyote food use, particularly game species and livestock, over a large spatial and temporal extent. Contents of gastrointestinal tracts were identified from 263 coyotes opportunistically obtained from hunters, trappers, and as road-kills throughout Florida, 2011-2015. We employed logistic regression in an information-theoretic framework to understand determinants of coyote food use. Coyotes were opportunistic and omnivorous foragers with a diverse diet of vegetation, insects, birds, reptiles, amphibians, and more than 25 species of mammals (including important game species and livestock). They commonly consumed 11 food items (Virginia opossum [Didelphis virginiana], non-mast vegetation, feral hog [Sus scrofa], northern raccoon [Procyon lotor], insects, rabbits (Sylvilagus spp.), skunks [Mephitis mephitis and Spilogale putorius], white-tailed deer (Odocoileus virginianus), mast, birds, and cows [Bos taurus]). Food use was determined by coyote age, sex, and body mass, season of the year, deer hunting and fawning seasons, livestock calving season, and coyote collection method and location/region. As coyotes expand their range and numbers, conservationists may find it useful to understand how this opportunistic and adaptable predator uses available food sources to reduce conflict across the landscape.
Florida Turkey Nest Site Selection and Success  [PDF]
William M. Giuliano, Lauren N. Watine, John M. Olson, Mitchell Blake, Holly Ober
Natural Resources (NR) , 2016, DOI: 10.4236/nr.2016.711051
Abstract: Changing landscapes and land-use practices are altering habitat for Florida wild turkeys (Meleagris gallopavo osceola). However, an understanding of habitat determinants of nest success is lacking for this unique turkey subspecies, potentially limiting conservation success. We examined female wild turkey nest site selection and nest success at microhabitat and patch levels using logistic regression in an Information-Theoretical framework in Florida, 2008-2010. We captured and radio-equipped adult female turkeys, and followed birds to nests. Nests were monitored to document success, and habitat was measured at multiple levels at nest and random sites. Females selected nest sites in dense vegetation (i.e., increased saw palmetto cover [Serenoa repens] and higher palm stem densities) that may have provided lateral and vertical cover for concealment at the microhabitat level (i.e., area within 7 m of the nest), while selecting for a more open habitat (i.e., decreasing hardwood and conifer stem densities) at the patch level (i.e., area within 28 m of the nest). Similarly, successful nests were in more dense vegetation at the nest site (i.e., increased saw palmetto cover) in an otherwise more open habitat (i.e., lower basal area) than unsuccessful nests. Habitat management that creates patches of dense shrub vegetation such as saw palmetto within an open landscape may be best for Florida wild turkey nesting habitat and success.
Characteristics of a primate model of focal motor cortical seizures suitable for preclinical testing of therapies like DBS  [PDF]
Shivadatta Prabhu, Brigitte Piallat, Annaelle Devergnas, Thomas Blauwblomme, Ariana Sherdil, Nathalie Chivoret, Olivier David, Stéphan Chabardès
World Journal of Neuroscience (WJNS) , 2014, DOI: 10.4236/wjns.2014.41006

Background and objective: Generating and characterizing primate models of epileptic seizures are important for understanding pathophysiology of diseases and establishing preclinical efficacy of novel therapies like Deep Brain Stimulation. A focal motor epilepsy model is described here. Method: Seizures were obtained after intracortical penicillin injection into the motor strip through a cannula in two awake monkeys and electrocorticograms were recorded by epidural screws. Seizures were analyzed and compared for number, average duration of each seizure and total duration of ictal activity. Pharmaco-resistance for antiepileptic drug was tested by administration of Diazepam during seizures. Results: A motor status with seizures mimicking those seen in Kojevnikov’s syndrome was easily generated several minutes after penicillin injection and lasted 24 h on an average. The model thus characterized appears stable and consistent. There is no significant variation between experiments in individual primate as well as between two specimens. Diazepam though reduced the total duration of seizures, failed to abolish behavioural seizures. Conclusion: This model represents a good alternative model for preclinical research aiming at testing novel therapies because seizures are obtained on demand, last up to 24 h after a single penicillin injection, are stable and resistant to Diazepam.

Phenotypic plasticity of body pigmentation in Drosophila: correlated variations between segments
Patricia Gibert, Brigitte Moreteau, Samuel M Scheiner, Jean R David
Genetics Selection Evolution , 1998, DOI: 10.1186/1297-9686-30-2-181
Combinatorial pseudo-Triangulations
David Orden,Francisco Santos,Brigitte Servatius,Herman Servatius
Mathematics , 2003, DOI: 10.1016/j.disc.2005.09.045
Abstract: We prove that a planar graph is generically rigid in the plane if and only if it can be embedded as a pseudo-triangulation. This generalizes the main result of math.CO/0307347 which treats the minimally generically rigid case. The proof uses the concept of combinatorial pseudo-triangulation, CPT, in the plane and has two main steps: showing that a certain ``generalized Laman property'' is a necessary and sufficient condition for a CPT to be ``stretchable'', and showing that all generically rigid plane graphs admit a CPT assignment with that property. Additionally, we propose the study of combinatorial pseudo-triangulations on closed surfaces.
Which Are the Factors Associated to Child Mistreatment? Use of a Routinely Collected Specific Dataset for Surveillance in a Belgian Pediatric Unit  [PDF]
Christelle Senterre, Brigitte Vanthournout
Health (Health) , 2014, DOI: 10.4236/health.2014.612171
Abstract: Introduction: Child abuse is a serious health problem with compelling evidence that the phenomenon is common throughout the world. The Center “SOS enfants ULB” is a specialized team, established in a pediatric ward, which had mission to prevent and to treat the situations of children victims of physical, sexual, psychological abuse or of neglect. Our objectives were to describe the characteristics of 439 children hospitalized for (suspicion of) maltreatment and to investigate different factors potentially associated with the children at risk of neglect or maltreatment, the children victims of neglect and the physically maltreated children. Methods: Chi square test and multinomial logistic regression models with clustered robust standard error were applied to assess the relationship between the three types of mistreatment and the potential associated factors. Results: Maltreatment was observed for almost one in two children (48.7%) and approximately four on ten (41.5%) were considered at risk. Physical abuse was the most prevalent (57.9%) among the maltreated children and neglect was the second most prevalent (37.4%) form of maltreatment. Regarding the criteria leading to hospitalisation, at least one protective criterion was observed for a little more than eight children on ten. The parental criteria have shown that social problems and conjugal conflicts were the most prevalent for this category of hospitalisation criteria. Conclusion: These data, concerning the hospitalized children for which there is (suspicion of) mistreatment, collected by this team make important contribution to describing child maltreatment and its associated factors. Despite the fact that these hospitalized cases may be probably more serious than those who were not reported, every event, however small it may be, should be known for better organizing each level of prevention.
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