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β-lactamase Molecular Classes Linked to Production Ways from Clinical Isolates in Burkina Faso/West Africa
Boukare Zeba,Jacques Simpore,Odile Germaine Nacoulma
Journal of Applied Sciences , 2007,
Abstract: β-lactamase production ways and inhibition patterns were investigated in cell suspensions of clinical isolates. The purpose of this research was prior to investigate the different β-lactamase molecular classes occurring in Burkina Faso owing to the local practice of β-lactam antibiotics. The use of specific inhibitors enabled to draw up an inhibition profile and consequently to assign an enzyme to accurate molecular class of β-lactamase. At the same time, β-lactamase expression ways were explored and correlated to the inhibition profiles. The current results pointed out two main groups of organisms. The first group of isolates secretes β-lactamases mainly by inducible way. Interestingly this bacterial group carries molecular class C of β-lactamase. The second group of isolates uses partial inducible and partial constitutive way for enzyme expression. These bacteria mostly carry molecular class A of β-lactamases. The sole exception encountered during these investigations is an organism, expressing exclusively by the constitutive way an enzyme that is found to belong to molecular class B of β-lactamases.
Antimicrobial Resistance and Serotype Distribution of Streptococcus pneumoniae Strains Causing Childhood Infection in Burkina Faso
Leonard C. Bere,Jacques Simpore,Simplice D. Karou,Boukare Zeba
Pakistan Journal of Biological Sciences , 2009,
Abstract: In Burkina Faso, a Western African country, reports on pneumococci carriage, resistance patterns and serotypes are inconsistent. The present study was conducted in order to evaluate these parameters. Thus 860 nasopharyngeal swabs were collected from children attending vaccination centers for pneumococci isolation, identification and serotype determination. The susceptibility to 16 antibiotics was assayed as recommended by the National Committee for Clinical Laboratory Standard (NCCLS). The results revealed that the majority of children were of 2 to 24 months age and 73.4% of children were well vaccinated. A carriage rate of 50.6% was recoded among the children. The main serotypes were: 6 (22.22%); 23 (16.67%); 7 and 9 (3.70%); 4, 11, 14, 15, 20 and 24 (1.85%). Serotypes19, 23, 6, 7 and 18 were linked to penicillin resistance. Globally, high resistance rates to: amikacin, tetracyclin, pefloxacin, cotrimoxazol and penicillins (resistance rates greater than 25%) were recorded; however the following antibiotics remained active on the strains: rifampicin, ceftriaxone, erythromycin, spectinomycin, chloramphenicol, vancomycin, lincomycin and ciprofloxacin.
Major Enzymatic Factors Involved in Bacterial Penicillin Resistance in Burkina Faso
Boukare Zeba,Martin Kiendrebeogo,Aline Lamien,Jean-Denis Docquier
Pakistan Journal of Biological Sciences , 2007,
Abstract: Many clinical species of bacteria were isolated from biological samples such as urines, blood and wound in Saint Camille medical centre of Ouagadougou. Among the concerned species, the most important members were Escherichia coli and Klebsiella pneumoniae. These β-lactamases producing isolates were directly screened by PCR to identify the nature of the amplified genes responsible for penicillin destroying activity. Therefore specific TEM and SHV primers were used. The PCR products were sequenced. The sequencing results indicated that the parental forms blaTEM-1 and blaSHV-1 were the most common determinants of β-lactamase found, respectively in Escherichia species and Klebsiella pneumoniae. The bacterial susceptibility analysis by MICs measurement clearly correlated the presence of concerned β-lactamase determinants and their resistance patterns. This study is part of a set of investigations carried out by our laboratory to assess the β-lactamase incidence in the failure of β-lactam therapy. In particular, the purpose of this study was to determine the precise nature of β-lactamase supporting the low susceptibility of host bacteria towards penicillins.
Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso
Aline Lamien-Meda,Charles Euloge Lamien,Moussa M.Y. Compaoré,Roland N.T. Meda,Martin Kiendrebeogo,Boukare Zeba,Jeanne F. Millogo,Odile G. Nacoulma
Molecules , 2008, DOI: 10.3390/molecules13030581
Abstract: A total of fourteen (14) species of wild edible fruits from Burkina Faso wereanalyzed for their phenolic and flavonoid contents, and their antioxidant activities usingthe DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic andtotal flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana andLannea microcarpa. Significant amounts of total phenolics were also detected in the otherfruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit alsoshowed the highest antioxidant activity using the three antioxidant assays. Fruits with highantioxidant activities were also found to possess high phenolic and flavonoid contents.There was a strong correlation between total phenolic and flavonoid levels and antioxidantactivities.
Overview of -lactamase incidence on bacterial drug resistance
B Zeba
African Journal of Food, Agriculture, Nutrition and Development , 2004,
Abstract: The bacterial drug resistance is its ability to grow in presence of high concentration of an antibiotic. The massive and often unsuitable use of antibiotic tends to select the resistant mutants that are then disseminated. The ß-lactam antibiotics represent an antibiotic family widely used because of their remarkable tolerance by animal organism. This practice generated the resistance from many bacteria in clinical and environmental spaces. One of the main drug resistance mechanism used by bacteria is the production of inactivation enzymes named ß-lactamases. This mini review attempts to pinpoint the ß- lactamase incidence on the failure of clinical treatment using ß-lactam antibiotic anywhere.
Electrochemical Sensors Based on Modification of Carbon Fiber Microelectrode by Nickel Phthalocyanine Polymer for 3-Methyl-4-Nitrophenol Analysis in Water  [PDF]
Yibor Fabrice Roland Bako, Boukare Kabore, Issa Tapsoba
Materials Sciences and Applications (MSA) , 2017, DOI: 10.4236/msa.2017.811058
Abstract: 3-methyl-4-nitrophenol (MNP) is the main by-product of the organophosphate insecticide fenitrothion (FT), used in locust control. MNP is highly toxic because it is an endocrine disruptor and then may cause adverse in the biological systems. Then, it is necessary to develop analytical methods for determination of this pollutant in the environment. In this sense, we reported herein the development of an electrochemical sensor for the detection of 3-methyl-4-nitrophenol (MNP), one of the metabolites of fenitrothion (FT), by using naked and modified carbon fiber microelectrode (CFME) by nickel tetrasulfonated phthalocyanine polymer (CFME/p-NiTSPc). The voltammogram showed that MNP presents one irreversible anodic peak corresponding to the oxidation of the phenol group at 0.9 V vs Ag/AgCl. The effect of pH of the buffer on the peak current and SWV parameters such as frequency, scan increment and pulse amplitude were investigated in order to optimize the electrochemical response of the sensor. The obtained results lead to the following optimum value: pH = 6; frequency = 25 Hz, pulse amplitude = 50 mV, scan increment = 10 mV. With these optimum values, the calibration curves show that the peak current varied linearly upon MNP concentration leading to a limit of detection (LoD) for naked CFME close to 3 μg/L whereas for CFME modified by p-NiTSPc, it reaches 0.75 μg/L. This results prove that the presence of p-NiTSPc increasing the sensitivity of the sensor could be used to monitor 3-methyl-4-nitrophenol residue in real matrix.
Using Topology of the Metabolic Network to Predict Viability of Mutant Strains
Zeba Wunderlich, Leonid Mirny
Genome Biology , 2006, DOI: 10.1186/gb-2005-6-13-p15
Abstract: We reconcile these seemingly conflicting results by showing that the topology of E. coli's metabolic network is, in fact, sufficient to predict the viability of knock-out strains with accuracy comparable to FBA on a large, unbiased dataset of mutants. This surprising result is obtained by introducing a novel topology-based measure of network transport: synthetic accessibility. We also show that other popular topology-based characteristics like node degree, graph diameter, and node usage (betweenness) fail to predict the viability of mutant strains. The success of synthetic accessibility demonstrates its ability to capture the essential properties of the metabolic network, such as the branching of chemical reactions and the directed transport of material from inputs to outputs.Our results (1) strongly support a link between the topology and function of biological networks; (2) in agreement with recent genetic studies, emphasize the minimal role of flux re-routing in providing robustness of mutant strains.Additional data files 1, 2 and 3.
Fictive-friendship and the Fourth Gospel
Zeba A. Crook
HTS Theological Studies/Teologiese Studies , 2011, DOI: 10.4102/hts.v67i3.997
Abstract: The phenomena of friendship and giftship in antiquity have been the focus of much anthropological interest, yet those terms are still used much too broadly, wherein any one can be friends and anything exchanged is a gift. This article argued that proper friendship requires equality of exchange and status. When inequality of exchange is present, we will almost always also have inequality of status. These two things together naturally and necessarily result in the absence of frank speech. At this point, proper friendship (defined by frank speech) and the exchange of gifts (defined by equality of value) are impossible, and we have fictivefriendship, a term I have introduced in this article. Fictive-friendship refers to the practice, often but not exclusively amongst elites, of using friendship language to mask relationships of dependence (patronage and clientage). I closed my argument by looking at two examples of fictive-friendship in the Gospel of John. How to cite this article: Crook, Z.A., 2011, ‘Fictivefriendship and the Fourth Gospel’, HTS Teologiese Studies/Theological Studies 67(3), Art. #997, 7 pages. http://dx.doi.org/10.4102/hts.v67i3.997
Prospects of Family Planning in India: An Empirical Analysis
Zeba Nisar,Tarique Hassan
Indian Streams Research Journal , 2012,
Abstract: This paper uses the NFHS-3 (2005-06) survey data on family planning to examine the prospects of family planning among different religious groups of the country by emphasizing on the issues related to the perception and knowledge of contraception, and the means of exposures to family planning messages, and its needs at the present scenario. The study reveals that the extent of knowledge of contraceptive methods has been highest in the religion of Buddhism and Jainism, while, the Christians witnessed the lowest knowledge of contraceptive methods among all the major religious communities. Moreover, there were significant differentials in the adoption of sterilization between each of the religious groups, highlighting the lowest use of sterilization by Muslim women and highest by Buddhist women. In addition, the frequency of use of any modern method at the time of sex is lowest in Muslims.
Food Insecurity And Child Labour In Aligarh City: A Case Study
Syed Naushad Ahmed,Zeba Nisar
Indian Streams Research Journal , 2012,
Abstract: The present study is undertaken at micro level in order to examine the level of food insecurity and to analyze the socio-economic conditions of child labour. This paper is based on the analysis of primary data, collected with the help of questionnaire to the respondents, carried out during the month of January and February, 2011. On the basis of purposive sampling method, 90 households have been selected from two selected wards of Aligarh city for detailed study. The survey has covered those vulnerable households which provide a classic case to assess the current status of household food insecurity and child labour. Ward is considered as the smallest unit of study. These wards of Aligarh city have the threat of food insecurity and household economic pressure which compel children to enter into low-wage, hazardous work environment that proves detrimental to their educational and health prospects.
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