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Experimental murine schistosomiasis mansoni: estabilishment of the chronic phase of the disease
Borojevic, Radovan;
Memórias do Instituto Oswaldo Cruz , 1992, DOI: 10.1590/S0074-02761992000800026
Abstract: after the acute hyperergic phase of schistosomal infection, the chronic phase of the disease corresponds to the estabilishment of a relative equilibrium between the host and the parasite. this involves: (1) a shift from the predominance of the th2 response observed in the acute phase, to the predominance of the th1 response in the chronic phase of the disease, with modification of lymphokine and immunoglobulin secretions patterns. (2) redistribution of hosts responses to parasite, with predominance of systemic controls in the acute phase, and a shift towards local tissue responses in the chronic phase. this redistribution relieves the hyperergic responses involving the whole body of the host, and delimits cellular and molecular reactions to parasites to only those tissues that are directly involved by the adult parasites and their eggs. mobilization of eosinophil granulocytes in schistosomal periovular granulomas is one of examples of this redistribution.
Extracellular matrix: understanding the complexity
Borojevic R.
Brazilian Journal of Medical and Biological Research , 1999,
麦类作物学报 , 1982, DOI: 10.7606/j.issn.1009-1041.1982.01.007
Abstract: 为了鉴定叶锈菌(Pucciniareconditatritici)的抗性,作者以早期实验中按数量性状作为突变体,从Sanpastore中选出的突变品系M_(12)、M_(13)、M_(14)为材料,以S.pastore为对照,进行了抗小麦叶锈菌的实验(菌种统一编号为8号小种和13号小种)。并在人工接种发病的小区内测定其反应型及严重度。通过温室内一次鉴定和田间三次鉴定的结
Terapias celulares do miocárdio com células da medula óssea: critérios de qualidade e perspectivas
Rossi, Maria Isabel D.;Borojevic, Radovan;
Revista Brasileira de Hematologia e Hemoterapia , 2009, DOI: 10.1590/S1516-84842009005000033
Abstract: cell therapy may provide a novel therapeutic option for cardiac patients, modifying myocardium remodeling processes and preventing post-infarction heart failure. currently clinical studies predominantly use bone marrow mononuclear cells isolated by density gradient centrifugation of iliac crest bone marrow aspirates. although this revolutionary new strategy seems to be safe and to improve myocardial function, negative data have emerged challenging the future of cell-based therapy for heart repair. here we discuss some laboratory data that might explain, at least in part, variations in outcomes using similar protocols. analysis of the correlation between the cell composition of the mononuclear fraction of bone marrow aspirates and the clinical outcome of the therapy has indicated that cells of the lymphocyte lineage are not beneficial in myocardial regeneration. a proposal of selection to eliminate these cells may improve cell therapy of infarcted myocardium.
Postoperative Radiotherapy in Early and Operable Breast Cancer
Jasmina Mladenovic,Nenad Borojevic,Dusan Mileusnic
Acta Medica Saliniana , 2012, DOI: 10.5457/ams.170.10
Abstract: Breast radiotherapy (RT) has changed over the passed few decades mainly due to changes in locoregional treatment of the breast cancer and improvements in technology of radiotherapy equipment. It has now become the standard part of the breast conserving procedure, as well as in patients who underwent mastectomy with T3 and/or 4 or more positive nodes in axilla. In treatment of ductal carcinoma in situ, postoperative RT after lumpectomy is almost always required, because it reduces ipsilateral invasive and DCIS recurrence by approximately 50-60%. For invasive breast cancer RT decreases the locoregional relapse rate by 70%. The indication for postoperative RT and the definition of the target volumes depend on the prognostic factors and surgical procedures. The overview by the Early Breast cancer Trialists’ Collaborative Group (EBCTCG) demonstrates for the first time, that postoperative RT is not only important in achieving loco-regional control, but also has significant influence on long-term survival. This benefit of postoperative RT is achieved by proper indication of RT and more importantly by using modern RT techniques that can avoid the serious late side-effects.
麦类作物学报 , 1990, DOI: 10.7606/j.issn.1009-1041.1990.04.054
Abstract: 以现代小麦品种为基础,运用基因-性状概念选择杂交亲本,对培育高产品种起到了良好效果。用意大利品种作为半矮秆株型的Rht矮生基因源,以美国品种作为早熟、穗丰产性好及抗病基因的供体,再用苏联矮秆品种作为耐寒与优质基因供体,通过单交、三交及渐近杂交法选育小麦新品种最为有效。在1960-1985的25年间,南斯拉夫的诺维萨德地区的育种工作取得了卓越的成效,共登记了137个品系,其中73个(即53%)是作为新品种进行登记的,即平均每年有2.92个品种准予登记。
Long-term culture of cholangiocytes from liver fibro-granulomatous lesions
Luciana B Chiarini, Christina M Takiya, Radovan Borojevic, Alvaro NA Monteiro
BMC Gastroenterology , 2006, DOI: 10.1186/1471-230x-6-13
Abstract: We have isolated a cholangiocyte cell line from Schistosoma-induced liver granulomas using a combination of methods including selective adhesion and isopyknic centrifugation in Percoll.The cell line was characterized by morphological criteria in optical and transmission electron microscopy, ability to form well differentiated ductular structures in collagen gels and by a positive staining for cytokeratin 18 and cytokeratin 19. To our knowledge, this is the first murine cholangiocyte cell line isolated from schistosomal fibrosis reported in the literature.After 9 months and 16 passages this diploid cell line maintained differentiated characteristics and a high proliferative capacity. We believe the method described here may be a valuable tool to study bile duct changes during hepatic injury.Extensive bile duct proliferation is a key feature of the tissue reaction to clinical and experimental forms of liver injury and in many cases, this proliferation may affect liver function [1,2]. It has long been appreciated that bile duct epithelial cells can be isolated and cultured in vitro from human [3-7] and animal liver tissue [8-12]. Cultures of bile duct epithelia have been derived from normal, cholestatic, or carcinogen-treated livers [10,11]. Although experimental infection of mice by Schistosoma mansoni is a well studied model of liver fibrosis with bile duct hyperplasia [13](Fig. 1A–F), cholangiocytes have not yet been isolated from schistosomal livers and characterized in vitro. In experimental schistosomiasis, a spectrum of pathologic changes of the intrahepatic bile ducts can be observed, such as hyperplasic epithelial lining made up of hypertrophic cells or cells with nuclei disposed in variable height and periductular fibrosis [13]. The origin and nature of these bile duct cells remain unknown since there has been no systematic study of the cells implicated in bile duct hyperplasia during Schistosoma infection.Since certain chronic disorders of the biliary tract
Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines
Anderson Teodoro, Felipe Oliveira, Nathalia Martins, Guilherme de Maia, Renata Martucci, Radovan Borojevic
Cancer Cell International , 2012, DOI: 10.1186/1475-2867-12-36
Abstract: Human cell lines were treated with lycopene (1–5?μM) for 48 and 96?h. Cell viability was monitored using the method of MTT. The cell cycle was analyzed by flow cytometry, and apoptotic cells were identified by terminal deoxynucleotidyl transferase-mediated dUTP nick labeling (TUNEL) and by DAPI.Our data showed a significant decrease in the number of viable cells in three cancer cells lines (HT-29, T84 and MCF-7) after 48?h treatment with lycopene, and changes in the fraction of cells retained in different cell cycle phases. Lycopene promoted also cell cycle arrest followed by decreased cell viability in majority of cell lines after 96?h, as compared to controls. Furthermore, an increase in apoptosis was observed in four cell lines (T-84, HT-29, MCF-7 and DU145) when cells were treated with lycopene.Our findings show the capacity of lycopene to inhibit cell proliferation, arrest cell cycle in different phases and increase apoptosis, mainly in breast, colon and prostate lines after 96?h. These observations suggest that lycopene may alter cell cycle regulatory proteins depending on the type of cancer and the dose of lycopene administration. Taken together, these data indicated that the antiproliferative effect of lycopene was cellular type, time and dose-dependent.Diets high in fruits and vegetables are associated with reduced rates of cancer and coronary heart disease. Lycopene, a major carotenoid component of tomato, exhibited potential anticancer activity in many types of cancer [1,2]. Epidemiological studies reported statistically significant inverse association between tomato consumption and risk of several types of cancer such as lung, prostate and colon cancer [3-5].Despite epidemiological and small clinical trial evidence suggesting a possible protective effect of lycopene, the mechanism of its action including cell cycle arrest and induction of apoptosis, remain poorly understood [6,7]. Lycopene has been proposed to negatively affect cancer cells or developmen
TGF β1 and PDGF AA override Collagen type I inhibition of proliferation in human liver connective tissue cells
Alvaro T Geremias, Marcelo A Carvalho, Radovan Borojevic, Alvaro NA Monteiro
BMC Gastroenterology , 2004, DOI: 10.1186/1471-230x-4-30
Abstract: In this study we address whether cells from fibrotic liver patients respond to normal controls of proliferation. We compared cell proliferation of primary human liver connective tissue cells (LCTC) from patients with liver fibrosis and skin fibroblasts (SF) in the presence of collagens type I and IV; TGF-β, PDGF AA and combinations of collagen type I and TGF-β or PDGF AA.Our results indicate that despite displaying normal contact and collagen-induced inhibition of proliferation LCTC respond more vigorously to lower concentrations of PDGF AA. In addition, we show that collagen type I synergizes with growth factors to promote mitogenesis of LCTC but not SF.The synergistic interaction of growth factors and extracellular matrix proteins may underlie the development of chronic liver fibrosis.In normal liver, connective tissue cells are rare and mostly restricted to the periportal and pericentrovenular spaces and to the Glisson's capsule. A minor population of connective tissue cells is present inside the hepatic lobule, in the perisinusoidal space. They are known as hepatic stellate cells (HSC) and are considered to be one of the major contributors for fibrogenesis in liver [1]. Chronic and acute injuries to liver tissue induce a marked expansion of the connective tissue cells and concomitantly an abnormal deposition of extracellular matrix proteins [2]. Extensive studies of experimental models and humans have shown that these cells are of the myofibroblast lineage, characterized by the expression of smooth muscle α-actin [3-5]. In fact, there is now increasing evidence that are several populations of myofibroblasts in the diseased liver in addition to those derived from HSC [6,7]. The origin of these cells is still debated. In experimental models, it is considered that an acute liver injury is followed by activation and increase of stellate cells, while chronic injuries elicit activation of similar cells that can be either of lobular or periportal origin [7]. In humans,
Milky spots reactions to schistosomal mansoni infection
Lenzi, H. L.;Oliveira, D. N.;Borojevic, R.;Lenzi, J. A.;
Memórias do Instituto Oswaldo Cruz , 1992, DOI: 10.1590/S0074-02761992000900017
Abstract: milky spots (ms), considered by the authors as a coelomatic lympho-myelopoietic organ (clmo), present a strong reactivity during experimental schistosomal mansoni infection, characterized by an increase of lymphocytes, macrophages, plasmocytes, mast cells, neutrophils and expression of eosinophil metaplasia. intraperitoneal injection of purified schistosoma mansoni (sm) eggs provoked a rise in the number and size of ms, which developed the sessile marginal and pedunculated types. the authors conclude that egg antigens are, at least partially, responsible for ms reactivity during sm infection.
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