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Search Results: 1 - 10 of 35782 matches for " Borja Hernández-Breijo "
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Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma
Borja Hernández-Breijo,Jorge Monserrat,Sara Ramírez-Rubio,Eva P Cuevas
World Journal of Gastroenterology , 2011, DOI: 10.3748/wjg.v17.i34.3899
Abstract: AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) by localized application into HepG2 tumor in vivo. METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 °C incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the efficacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase. RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO. CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localized treatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method.
Fundamentos didácticos de la ense?anza de la Historia en la Educación Médica Superior
Barrera Breijo,Martha Elena; Hernández Rodríguez,Ileana María; Díaz Lobo,Luis Manuel;
Revista de Ciencias M??dicas de Pinar del R?-o , 2012,
Abstract: teaching the history of cuba in medical universities responds to a need of the country in order to train health professionals, having not only a high scientific level but also prepared to comprehend the current events to identify with the people. a literature review and a classification of professors of history at the medical university in pinar del rio were the starting points of this study; which allowed the definition of the didactic basis where the teaching of the subject must be supported nowadays, constituting at the same time the theoretical base to be applied in the whole context of the medical training since the historical formation of the medical students is assumed as an essential pillar to the model of health professionals.
Fundamentos didácticos de la ense anza de la Historia en la Educación Médica Superior Didactic basis to teach History of Cuba in Higher Medical Education
Martha Elena Barrera Breijo,Ileana María Hernández Rodríguez,Luis Manuel Díaz Lobo
Revista de Ciencias M??dicas de Pinar del R?-o , 2012,
Abstract: La ense anza de la Historia de Cuba en las Universidades de Ciencias Médicas responde a una necesidad del país de formar profesionales de la salud, no solo con un elevado nivel científico sino además preparados para comprender su contemporaneidad e identificados con su pueblo. El trabajo se realizó a partir una revisión bibliográfica y de la caracterización de los profesores de Historia que trabajan en la Universidad de Ciencias Médicas de Pinar del Río lo que permitió definir los fundamentos didácticos en los que hoy debe sustentarse la ense anza de la Historia de Cuba y que constituyen basamento teórico para aplicar en todo contexto de formación médica donde se asume que la formación histórica del estudiante de las carreras de las Ciencias Médicas es pilar esencial en el modelo de este profesional. Teaching the History of Cuba in Medical Universities responds to a need of the country in order to train health professionals, having not only a high scientific level but also prepared to comprehend the current events to identify with the people. A literature review and a classification of professors of History at the Medical University in Pinar del Rio were the starting points of this study; which allowed the definition of the didactic basis where the teaching of the subject must be supported nowadays, constituting at the same time the theoretical base to be applied in the whole context of the medical training since the historical formation of the medical students is assumed as an essential pillar to the model of health professionals.
Fundamentos teóricos para el dise o de una estrategia metodológica para la preparación de los profesores de Historia en las universidades Theoretical basis to the design of a methodological strategy to the training of professors of History in the universities
Martha Elena Barrera Breijo,Ileana María Hernández Rodríguez,Maite Reinoso Díaz
Revista de Ciencias M??dicas de Pinar del R?-o , 2013,
Abstract: Introducción: la preparación de los docentes que imparten Historia en la universidades requiere una especial atención por lo que aportan estas clases a la formación de los futuros profesionales. Objetivo: fundamentar el dise o de una estrategia metodológica que garantice la preparación de los profesores para impartir la Historia en el contexto de la educación superior. Material y método: investigación cualitativa dirigida a la preparación metodológica de los profesores de Historia de la Carrera de Medicina de la Universidad de Ciencias Médicas de Pinar del Río. Sustentada en el método dialéctico materialista empleó métodos teóricos: el histórico-lógico y enfoque sistémico. De los métodos empíricos se aplicó una encuesta y una guía de observación a clases. Se utilizó la estadística descriptiva y se empleó el criterio de expertos. Resultados: el 37,9 % (11) de los docentes reconocen sus dificultades en el conocimiento de la Didáctica de la Historia. El 47,3% (12) participó en cursos especializados, el 6,8 % (2) transitó por figuras superiores del postgrado. El 47,3% (12) conceden importancia al conocimiento del modelo del profesional lo que se corresponde con que el 20,6 % (6), de ellos, conocen los elementos que forman parte de dicho modelo. Conclusiones: la estrategia metodológica determinada garantiza que, las acciones propias para su desarrollo, el sistema de trabajo metodológico y la elaboración de un curso de superación, constituyan sus procedimientos esenciales en función de la preparación metodológica del claustro de Historia en las universidades y garantiza impartir la disciplina en función del modelo del profesional. Introduction: the teaching staff of History subject in universities requires a special training because of the contribution of this subject to the future medical professionals. Objective: to support the design of a methodological strategy that guaranties the training of professors to teach History subject in higher education contexts. Material and method: qualitative research towards the training of History subject professors in Medical Studies at the Medical University in Pinar del Rio, which was supported on materialist-dialectic methods using historic-logical and systemic approaches. A survey was applied together with an observation guide to lectures. Descriptive statistics was used with the criterion of experts. Results: 37,9% (11) of the teaching staff recognized their difficulties in learning the Didactics of History subject; 47,3% (12) participated in specialized courses, 6,8% (2) passed higher postgraduate courses. The 47,3%
El papel de la ense anza de la historia en el desarrollo comunicativo del profesional de la salud The Role of History teaching in the communicative development of the Health Professional
Martha E Barrera Breijo,Ileana M Hernández Rodríguez,Belkis Ferro González,Ana Ibis Garriga Due?as
Revista de Ciencias M??dicas de Pinar del R?-o , 2007,
Abstract: El presente trabajo introduce el tema en cuestión a partir del análisis de diferentes postulados teóricos sobre la comunicación y la importancia de la ense anza de la Historia con estos propósitos, en el contexto de la formación médica, que sirvió de base para el análisis documental y la elaboración de dos instrumentos que se aplicaron corroborando la necesidad de crear vías para el desarrollo de la comunicación, y la efectividad de la experiencia aplicada desde el Programa de Historia de Cuba III en 4to a o de la carrera de Medicina respectivamente. Para el desarrollo de estos propósitos se fundamentaron teóricamente las potencialidades de la ense anza de la asignatura y se ofreció un modelo recurrente que constituyó el estilo metodológico con que se impartieron las 16 actividades docentes del programa para dar cumplimiento al objetivo propuesto, demostrando que el dise o de las estrategias de ense anza aprendizaje debe estar encaminado a construir eslabones para el desarrollo de la comunicación, donde la ense anza de la Historia constituya un fuerte puntal en este propósito, además de contribuir a solucionar los problemas de comunicación que afloren en el período de formación médica e incorpore habilidades, métodos y conocimientos al desarrollo del método clínico, cuyos resultados se evidenciaron en el análisis cualitativo y cuantitativo del instrumento aplicado. The present work is based on the teaching of History from the analysis of different theoretical postulates on communication and the importance of History teaching in the medical context, which was used as a base for the documented analysis and the creation of two instruments which were applied, demonstrating the necessity of means for the development of communication and the effectiveness of the applied training from the Cuban History III in the 4 academic year of Medical major. With these purposes the possibilities of the subject teaching were based theoretically and it was offered a recurrent model which constituted the methodological style used for teaching 16 teaching activities of the program fulfilling the proposed objective which demonstrated that the design of the teaching- learning strategies must be directed to create links for the development of communication where the History teaching is a strong support and it also contributes to solve the communication problems which emerge in the medical formation period and gives training , methods and knowledges of the clinical method development whose results were evidenced in the qualitative and quantitative analysis of the applied instrume
Problemas clínicos durante el mantenimiento del donante potencial de órganos Clinical problems during the maintenance of potential organ-donors
Omar Martínez Mompeller,Ariadna García Rodríguez,Alina Breijo Puentes,Juan A Prieto Hernández
Revista de Ciencias M??dicas de Pinar del R?-o , 2004,
Abstract: Se realizó un estudio descriptivo y retrospectivo de los donantes de órganos para trasplantes en el Hospital Abel Santamaría Cuadrado de Pinar del Río en el período comprendido entre enero del 2000 y diciembre del 2003 para conocer los principales problemas durante el mantenimiento de los 23 donantes reales. Hubo un incremento del número de casos con el transcurso de los a os. El trauma cráneo encefálico fue la causa principal de muerte encefálica. La hipotensión arterial y las arritmias fueron los eventos hemodinámicos más reportados. Los disturbios hidroelectrolíticos más relevantes fueron la hipopotasemia y la hipernatremia. La alcalosis mixta fue la alteración del equilibrio ácido - básico de mayor incidencia. La hipotermia, la poliuria y la hiperglicemia constituyeron otras alteraciones también presentes. Demostramos la importancia de un estrecho seguimiento del donante para prevenir y/o tratar los problemas clínicos que se presentan en este tipo de paciente. La información recogida fue procesada y analizada con la utilización de frecuencias absolutas y porcentajes, los resultados obtenidos se mostraron en tablas para una mejor comprensión de los mismos permitiéndonos arribar a conclusiones finales y hacer recomendaciones pertinentes. A descriptive and retrospective study on donors of organs for transplantations was performed at Abel Santamaría Hospital in Pinar del Río in the period of January 2000 and December 2003 aimed at assessing the most significant disorders during the management of 23 potential donors. There was an increase in the number in cases in years. Cranioencephalic trauma was the main cause of encephalic death. Hypotension and dysrrythmias were the most relevant hemodynamic disorders as well as electrolyte imbalance (hypokalemia and hypernatremia); mixed alkalosis was the most significant disorder of the acid-base balance, hypothermia, polyuria and hyperglycemia were other present disorders. The importance of a close follow-up of the donors to prevent and/or manage clinical disorders of these kinds of patients was shown. The information recorded was processed and analyzed using absolute frequencies and percentages, the results obtained were registered in tables in order to understand conclusions and recommendations.
Outcome of the Use of a Synthetic Mesh in the Repair of Burst Abdomen as Compared with Simple Suture  [PDF]
Manuel López-Cano, José A. Pereira, Xavier Feliu, Lourdes Hernández, Daniel Andrada, Esther Gil, Borja Villanueva, Manuel Armengol-Carrasco
International Journal of Clinical Medicine (IJCM) , 2015, DOI: 10.4236/ijcm.2015.63016
Abstract:
Aim: To assess the outcome (postoperative morbidity and mortality and/or development of incisional hernia) of patients with burst abdomen (BA) of midline laparotomy without intestinal fistula comparing early closure of the abdominal wall with a running suture alone vs. running suture plus reinforcement with a non-absorbable synthetic mesh. Methods: Retrospective analysis of the medical records of all patients undergoing surgical repair of a BA in three hospitals between 2006 and 2011. Risk factors, postoperative complications and the incidence of incisional hernia (IH) after a minimum of 12-month follow-up period were compared between groups. Results: A total of 109 patients were treated for BA, 18 patients treated by non-standard procedures were excluded from analysis. Of the remaining 91 patients, 56 belong to the mesh group and 35 to the suture group. The overall rate of postoperative complications was high in both groups (79.1%). Mortality rate was higher in the suture group than in the mesh group (31.4% vs. 14.3%; P = 0.06). IH was also more frequent in the suture group (36.4% vs. 17.6%; P = 0.057). Conclusions: Patients with BA and without intestinal fistula could be candidates for surgical repair using a running suture and a non-absorbable polypropylene mesh in the “onlay” position.
Problemas clínicos durante el mantenimiento del donante potencial de órganos
Martínez Mompeller,Omar; García Rodríguez,Ariadna; Breijo Puentes,Alina; Prieto Hernández,Juan A; Ceballo Morejón,Yosbel; Martínez Fabregas,Armando;
Revista de Ciencias M??dicas de Pinar del R?-o , 2004,
Abstract: a descriptive and retrospective study on donors of organs for transplantations was performed at abel santamaría hospital in pinar del río in the period of january 2000 and december 2003 aimed at assessing the most significant disorders during the management of 23 potential donors. there was an increase in the number in cases in years. cranioencephalic trauma was the main cause of encephalic death. hypotension and dysrrythmias were the most relevant hemodynamic disorders as well as electrolyte imbalance (hypokalemia and hypernatremia); mixed alkalosis was the most significant disorder of the acid-base balance, hypothermia, polyuria and hyperglycemia were other present disorders. the importance of a close follow-up of the donors to prevent and/or manage clinical disorders of these kinds of patients was shown. the information recorded was processed and analyzed using absolute frequencies and percentages, the results obtained were registered in tables in order to understand conclusions and recommendations.
El papel de la ense?anza de la historia en el desarrollo comunicativo del profesional de la salud
Barrera Breijo,Martha E; Hernández Rodríguez,Ileana M; Ferro González,Belkis; Garriga Due?as,Ana Ibis; Rodríguez Maqueira,Alexis;
Revista de Ciencias M??dicas de Pinar del R?-o , 2007,
Abstract: the present work is based on the teaching of history from the analysis of different theoretical postulates on communication and the importance of history teaching in the medical context, which was used as a base for the documented analysis and the creation of two instruments which were applied, demonstrating the necessity of means for the development of communication and the effectiveness of the applied training from the cuban history iii in the 4 academic year of medical major. with these purposes the possibilities of the subject teaching were based theoretically and it was offered a recurrent model which constituted the methodological style used for teaching 16 teaching activities of the program fulfilling the proposed objective which demonstrated that the design of the teaching- learning strategies must be directed to create links for the development of communication where the history teaching is a strong support and it also contributes to solve the communication problems which emerge in the medical formation period and gives training , methods and knowledges of the clinical method development whose results were evidenced in the qualitative and quantitative analysis of the applied instrument.
CXCR4+ and SDF-1+ Bone Marrow Cells Are Mobilized into the Blood Stream in Acute Myocardial Infarction and Acute Ischemia  [PDF]
José Luis Aceves, Rafael Vilchis, María Antonieta Medina, Monserrat Borja, Silvia Cortes, Guillermo Díaz, Armando Castro, Alexis Gómez, José J. Parra, Martha Alvarado, Manuel López Hernández, Virna Poveda, Felipe Masso, Luis F. Monta?o
World Journal of Cardiovascular Diseases (WJCD) , 2014, DOI: 10.4236/wjcd.2014.47045
Abstract: Cell therapy has shown beneficial effects on ventricular function and tissue regeneration in patients with acute and chronic myocardial infarction, although with diverse grades of variability in the results, possibly by proportion, subtype and cell cycle status. Objective: Identify and phenotypically characterize, via CXCR4 and SDF-1 expression, the bone marrow cell subpopulations that are mobilized into the bloodstream in patients with Acute Myocardial Infarction (AMI) and Acute Ischemia (AI) such as acute angina and Chronic Ischemia (CI) such as chronic stable angina, and also determine the cell cycle status of these cells. Method: Patients with AMI and AI were recruited in the ICCU, and patients with CI in the departments of cardiology and cardiovascular surgery. The quantification of cellular subpopulations was made by cytofluorometry with a FACS caliburcyto fluorometry (Becton Dickinson) with specific FITC-labeled anti human monoclonal antibodies against CD34, CD133, CD117, CD48, CXCR4, SDF-1 and Ki67 (Becton Dickinson). Serum concentration of IL-6 and IL-8 were determined by a sequential solid phase chemiluminescent assay performed in a SIEMENS IMMULITE 1000 Analyzer. Statistical analysis was made with the SPSS version 20.0 for Windows. A p value < 0.05 was considered as statistical significant. Results: We analyzed 174 patients. 67 had Acute Myocardial Infarction, 55 Acute Ischemia and 52 Chronic Ischemia. Total cellularity of bone marrow and SDF-1+ cells was significantly higher in patients with AMI (14.6 ± 1.5 × 103/ml) than that in AI (9.2 ± 1.3 × 103/ml) and CI (6.6 ± 1.1 × 103/ml) patients (p < 0.001). There were no significant differences in the amount of CD34+, CD117+, CD133+ and CD48+ cells between AMI (49.9 ± 3.9, 45 ± 4.7, 43.2 ± 3.7, 35.4 ± 6.7 respectively) and AI (36.7 ± 2.5, 36 ± 3.2, 33.7 ± 5.1, 32 ± 5 respectively) patients (p = 0.22 to 0.39), but interestingly in AMI and AI patients, cells were CXCR4+ in almost half of these mobilized cells, although the proportion was significantly higher in AMI patients (46.8% ± 7.1% to 55.7% ± 6.3% vs 23% ± 1.6% to 28.4% ± 2.1%, p = 0.03 to 0.05). A similar behavior was observed with the Ki67 antibody (29.9% ± 2.1% to 36.1% ± 6.3% vs 10% ± 1.2% to 24% ± 1.1%, p = 0.001 to 0.05). Bivariate analysis of the results showed a significant correlation of the cell proportion in AMI but not in AI and CI patients (p = 0.001 to 0.05; 0.12 to
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