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Search Results: 1 - 10 of 36736 matches for " Bomin Yan "
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Antisense Oligonucleotide Against Clusterin Regulates Human Hepatocellular Carcinoma Invasion Through Transcriptional Regulation of Matrix Metalloproteinase-2 and E-Cadherin
Dong Chen,Yan Wang,Kejun Zhang,Xuelong Jiao,Bomin Yan,Jun Liang
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms130810594
Abstract: Secreted clusterin (sCLU) has been shown to be overexpressed in metastatic hepatocellular carcinoma (HCC) tissue, and its overexpression in HCC cells increases cell migration and the formation of liver metastatic tumor nodules in vivo. In this study, we tested the hypothesis that sCLU plays a role in the invasiveness of human HCC and may be associated with its metastatic spread. HCCLM3, a human hepatocellular carcinoma cell line, was transiently transfected with an antisense oligonucleotide (ASO) against sCLU (OGX-011). HepG2 liver hepatocellular cells were transiently transfected with the pc.DNA3.1-sCLU plasmid to overexpress sCLU, and subsequently evaluated for effects on invasion and the expression of molecules involved in invasion. We observed that suppression of the sCLU gene significantly reduced the invasive capability of the highly invasive HCCLM3 cells, and vice versa in the low invasive HepG2 cell line. The results revealed that knockdown of sCLU by OGX-011 resulted in a significant increase in the expression of E-cadherin and a decrease in matrix metalloproteinase-2 ( MMP-2) gene transcription. Overexpression of sCLU by transfection with pc.DNA3.1-sCLU significantly decreased the expression of E-cadherin and increased MMP-2 gene transcription. These data were further verified by reverse transcription-PCR and Western blot analysis. A significant reduction in MMP-2 expression and an increase in E-cadherin expression in sCLU-knockdown HCCLM3 cells were observed, as well as a significant increase in MMP-2 expression and a decrease in E-cadherin expression in HepG2 cells overexpressing sCLU. These data indicate a role for sCLU in augmenting MMP-2 transcription and decreasing E-cadherin expression. Our data show the involvement of sCLU in human HCC invasion, and demonstrate that silencing sCLU gene expression inhibits the invasion of human HCC cells by inhibiting MMP-2 expression and promoting E-cadherin expression. Thus, OGX-011 could be an effective therapeutic agent for HCC.
Correction: Liang, J., et al. Antisense Oligonucleotide Against Clusterin Regulates Human Hepatocellular Carcinoma Invasion Through Transcriptional Regulation of Matrix Metalloproteinase-2 and E-Cadherin. Int. J. Mol. Sci. 2012, 13, 10594-10607
Dong Chen,Yan Wang,Kejun Zhang,Xuelong Jiao,Bomin Yan,Jun Liang
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14036516
Abstract: The original version of the paper reports that “ OGX-011 is a second generation 21-mer oligonucleotide with a 20-O-(2-methoxy)-ethyl modification, generously provided by OncoGenex Technologies (OncoGenex, Vancouver, Canada)” [1] (p. 10602). OGX-011 was not provided by OncoGenex Technologies directly. Therefore, we would like to correct the wording to: “OGX-011 was obtained without the benefit of an agreement with OncoGenex, or The University British Columbia, or any other party”. The authors would like to apologize for any inconvenience this may have caused to the readers of this journal.
RNA Interference Targeting Slug Increases Cholangiocarcinoma Cell Sensitivity to Cisplatin via Upregulating PUMA
Kejun Zhang,Dong Chen,Xingang Wang,Shaoyan Zhang,Jigang Wang,Yuan Gao,Bomin Yan
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12010385
Abstract: Slug is an E-cadherin repressor and a suppressor of PUMA (p53 upregulated modulator of apoptosis) and it has recently been demonstrated that Slug plays an important role in controlling apoptosis. In this study, we examined whether Slug’s ability to silence expression suppresses the growth of cholangiocarcinoma cells and/or sensitizes cholangiocarcinoma cells to chemotherapeutic agents through induction of apoptosis. We targeted the Slug gene using siRNA (Slug siRNA) via full Slug cDNA plasmid (Slug cDNA) transfection of cholangiocarcinoma cells. Slug siRNA, cisplatin, or Slug siRNA in combination with cisplatin, were used to treat cholangiocarcinoma cells in vitro. Western blot was used to detect the expression of Slug, PUMA, and E-cadherin protein. TUNEL, Annexin V Staining, and cell cycle analysis were used to detect apoptosis. A nude mice subcutaneous xenograft model of QBC939 cells was used to assess the effect of Slug silencing and/or cisplatin on tumor growth. Immunohistochemical staining was used to analyze the expression of Slug and PUMA. TUNEL was used to detect apoptosis in?vivo. The results showed that PUMA and E-cadherin expression in cholangiocarcinoma cells is Slug dependent. We demonstrated that Slug silencing and cisplatin both promote apoptosis by upregulation of PUMA, not by upregulation of E-cadherin. Slug silencing significantly sensitized cholangiocarcinoma cells to cisplatin through upregulation of PUMA. Finally, we showed that Slug silencing suppressed the growth of QBC939 xenograft tumors and sensitized the tumor cells to cisplatin through PUMA upregulation and induction of apoptosis. Our findings indicate that Slug is an important modulator of the therapeutic response of cholangiocarcinoma cells and is potentially useful as a sensitizer in cholangiocarcinoma therapy. One of the mechanisms is the regulation of PUMA by Slug.
Two Important Features of the 156 Deadliest Tropical Cyclones (1851-1996) in the North Atlantic  [PDF]
Bomin Chen, Jiqing Tan, Huiyi Fan
Atmospheric and Climate Sciences (ACS) , 2019, DOI: 10.4236/acs.2019.91005
Abstract: Damages and deaths due to a tropical cyclone (TC) can easily occur at three places belong to a TC: the small ring area (SRA) of maximum sustaining wind around TC eye, the large ring area (LRA) of the rotating wind field around SRA and the skin air belt (SKAB) around LRA. However, weather forecasters only use the intensity of the SRA of a TC as the proxy to gauge the whole TC intensity, which led to many “non-major” TCs proved to be “deadly” TCs. This is called as “over-generalization problem” (OGP). Here we introduce a new way to measure the intensity of a TC. After investigating 156 deadly TCs in North Atlantic, we found an important feature: 83.3% of the top 156 deadliest TCs usually made landfalls along “five major tracks”. Furthermore, we found that the new intensity of those “deadliest” TCs kept almost unchanged with the increasing of time intervals at earlier stage from the genesis points, whereas the new intensity of those “safe” TCs increased in a line with much steeper slope. Using these two features, weather forecasters can have two more options to identify those “non-major” and “deadly” TCs by SSHS in future.
Clusterin confers gmcitabine resistance in pancreatic cancer
Qingfeng Chen, Zhengkun Wang, Kejun Zhang, Xiaoyi Liu, Weihong Cao, Lei Zhang, Shuhua Zhang, Bomin Yan, Yaoguang Wang, Chunping Xia
World Journal of Surgical Oncology , 2011, DOI: 10.1186/1477-7819-9-59
Abstract: Immunohistochemistry for clusterin was performed on 50 primary pancreatic cancer tissues and 25 matched backgrounds, and clusterin expression in 5 pancreatic cancer cell lines was quantified by Western blot and PT-PCR. The correlation between clusterin expression level and gmcitabine IC50 in pancreatic cancer cell lines was evaluated. The effect of an antisense oligonucleotide (ASO) against clusterin(OGX-011) on gmcitabine resistance was evaluated by MTT assays. Xenograft model was used to demonstrate tumor growth.Pancreatic cancer tissues expressed significantly higher levels of clusterin than did normal pancreatic tissues (P < 0.01). Clusterin expression levels were correlated with gmcitabine resistance in pancreatic cancer cell lines, and OGX-011 significantly decreased BxPc-3 cells resistance to gmcitabine (P < 0.01). In vivo systemic administration of AS clusterin and gmcitabine significantly decreased the s.c. BxPC-3 tumor volume compared with mismatch control ODN plus gmcitabine.Our finding that clusterin expression was significantly higher in pancreatic cancer than in normal pancreatic tissues suggests that clusterin may confer gmcitabine resistance in pancreatic cancer cells.Pancreatic cancer is resistant to almost all cytotoxic drugs. Currently, gmcitabine appears to be the only clinically active drug but, because of pre-existing or acquired chemoresistance of most of the tumor cells, it failed to significantly improve the outcome of pancreatic carcinoma patients [1].Clusterin, also known as testosterone-repressed prostate message-2 (TRPM-2), apolipoprotein J (ApoJ), sulfated glycoprotein-2 (SGP-2), and complement lysis inhibitor(CLI), was first isolated from ram rete testes fluid and plays important roles in various pathophysiological processes, such as tissue remodeling, lipid transport, complement regulation, and apoptosis [2]. Initially clusterin has been regarded as a marker for cell death because its expression is up-regulated in various normal and m
The “Ocean Stabilization Machine” May Represent a Primary Factor Underlying the Effect of “Global Warming on Climate Change”  [PDF]
Yanjun Mao, Jiqing Tan, Bomin Chen, Huiyi Fan
Atmospheric and Climate Sciences (ACS) , 2019, DOI: 10.4236/acs.2019.91009
Abstract: Contemporary references to global warming pertain to the dramatic increase in monthly global land surface temperature (GLST) anomalies since 1976. In this paper, we argue that recent global warming is primarily a result of natural causes; we have established three steps that support this viewpoint. The first is to identify periodic functions that perfectly match all of the monthly anomaly data for GLST; the second is to identify monthly sea surface temperature (SST) anomalies that are located within different ocean basin domains and highly correlated with the monthly GLST anomalies; and the third is to determine whether the dramatically increasing (or dramatically decreasing) K-line diagram signals that coincide with GLST anomalies occurred in El Niño years (or La Niña years). We have identified 15,295 periodic functions that perfectly fit the monthly GLST anomalies from 1880 to 2013 and show that the monthly SST anomalies in six domains in different oceans are highly correlated with the monthly GLST anomalies. In addition, most of the annual dramatically increasing GLST anomalies occur in El Niño years; and most of the annual dramatically decreasing GLST anomalies occur in La Niña years. These findings indicate that the “ocean stabilization machine” might represent a primary factor underlying the effect of “global warming on climate change”.
The Analysis on the Features of the Atmospheric Circulation in Preceding Winters for the Summer Drought and Flooding in the Yangtze and Huaihe River Valley
The Analysis on the Features of the Atmospheric Circulation in Preceding Winters for the Summer Drought and Flooding in the Yangtze And Huaihe River Valley

Sun Bomin,Sun Shuqing,
Sun Bomin
,Sun Shuqing

大气科学进展 , 1994,
Abstract: Based on the annual variation of the rainfall departure percentage in summer in the lower-middle reaches of the Yangtze River and the Huaihe River valley, 7 cases for the abnormal drought and flooding summers (the drought years: 1981, 1984, 1985; the flooding years: 1980, 1982, 1983, 1987) are selected, First we analyse the general circula-tion characteristics of the summer drought and flooding, and then the evolution processes of the general circulation patterns from preceding winters to summers are studied. It is found that during the two kinds of preceding winters for the drought and flooding summer, not only the general circulation patterns in the high-mid latitudes, the local Hadley cells in East Asia but also the activities of the cold surge in the lower latitude are different obviously. Spring, especially April, is the turning period of the general circulation in preceding winter for the drought or flooding sum-mer evolution towards opposite direction. Hereafter, the drought or flooding circulation pattern is established and developed.
Numerical Tests for Predicting the Precipitation and Vortices over the Tibetan Plateau in Summer

Chen Bomin,Qian Zhengan,

大气科学 , 1995,
Abstract: This paper first analyzes the differences of the wind and relative humidity fields of FGGE level-IIIb data set for the period from June to July 1979 over the Tibetan Plateau from the truth and reveals the necessity to revise the data set over the west of the Plateau.Then,the initial wind and relative humidity fields in a regional model is corrected,parts of its physical processes modified,and its horizontal resolution inereased.Six predicting experiments are designed.A set of 24-h forecasts of two Plateau vortex/shear line precipitation cases are carried out.The results show that:the precipitation forecast over the Plateau can be clearly improved by modifying thd initial field and model Physics.The now field can also be improved to some extent.This shows that the above modification schemes are feasible.However,the nesting scheme of the model over the Plateau remains to be modified.The next efforts should focus on improving the forecast skill for the Plateau vortex now field.
Higher order Voronoi based mobile coverage control
Bomin Jiang,Zhiyong Sun,Brian D. O. Anderson
Computer Science , 2014,
Abstract: Most current results on coverage control using mobile sensors require that one partitioned cell is the sole responsibility of one sensor. In this paper, we consider a class of generalized Voronoi coverage control problems by using higher order Voronoi partitions, motivated by applications that more than one senor is required to monitor and cover onecell. We introduce a framework depending on a coverage performance function incorporating higher order Voronoi cells and then design a gradient-based controller which allows the multi-sensor system to achieve a local equilibrium in a distributed manner. In addition, we provide a number of real world scenarios where our framework can be applied. Simulation results are also provided to show the controller performance.
Simultaneous Velocity and Position Estimation via Distance-only Measurements with Application to Multi-Agent System Control
Bomin Jiang,Mohammad Deghat,Brian D. O. Anderson
Computer Science , 2014,
Abstract: This paper proposes a strategy to estimate the velocity and position of neighbor agents using distance measurements only. Since with agents executing arbitrary motions, instantaneous distance-only measurements cannot provide enough information for our objectives, we postulate that agents engage in a combination of circular motion and linear motion. The proposed estimator can be used to develop control algorithms where only distance measurements are available to each agent. As an example, we show how this estimation method can be used to control the formation shape and velocity of the agents in a multi agent system. Simulation results are provided to illustrate the performance of the proposed algorithm.
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