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Search Results: 1 - 10 of 97 matches for " Biswanath Bhunia "
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Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042
Biswanath Bhunia,Apurba Dey
Enzyme Research , 2012, DOI: 10.1155/2012/905804
Abstract: The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitation technique. The HPLC analysis of dialyzed sample showed that the retention time is 1.84?min with 73.5% purity. This enzyme retained more than 92% of its initial activity after preincubation for 30?min at in the presence of 25%?v/v DMSO, methanol, ethanol, ACN, 2-propanol, benzene, toluene, and hexane. In addition, partially purified enzyme showed remarkable stability for 60?min at room temperature, in the presence of anionic detergent (Tween-80 and Triton X-100), surfactant (SDS), bleaching agent (sodium perborate and hydrogen peroxide), and anti-redeposition agents (Na2CMC, Na2CO3). Purified enzyme containing 10%?w/v PEG 4000 showed better thermal, surfactant, and local detergent stability. 1. Introduction The protease is ubiquitous in nature. It is found in all living organisms and required for cell growth and differentiation. Alkaline proteases are one of the most important groups of industrial enzymes. They are extensively used in leather, food, pharmaceutical, textile, organic chemical synthesis, wastewater treatment, and other industries [1]. Alkaline proteases hold a major share of the enzyme market with two-third share in detergent industry alone [2, 3]. Due to enhancement of such demand of proteases for specific properties, scientists are looking for newer sources of proteases. For effective use in industries, alkaline proteases need to be stable and active at high temperature and pH and in the presence of surfactants, oxidizing agents, and organic solvents [4–7]. Although there are many microbial sources available for protease production, only a few are considered as commercial producers [8]. Of these, species of Bacillus dominate in the industry [9]. Only large-scale production of alkaline protease can fulfill the demand and usefulness of the proteases in the industry. In industry, microbial protease production was carried out by fermentative process. It is necessary to improve the yield of protease without increasing the process cost through fermentative process. Rapid enzyme production can be achieved by manipulation of media composition and culture conditions. Thus, optimization of fermentation conditions is the most important step in the
Selection of Suitable Carbon, Nitrogen and Sulphate Source for the Production of Alkaline Protease by Bacillus licheniformis NCIM-2042
Biswanath BHUNIA,Debjani DUTTA,Surabhi CHAUDHURI
Notulae Scientia Biologicae , 2010,
Abstract: In this study, selection of suitable carbon, nitrogen and sulphate sources were carried out by one-variable-at-time approach for the production of alkaline protease enzyme by Bacillus licheniformis NCIM-2042. Maximum levels of alkaline protease were found in culture media supplemented with magnesium sulphate, starch and soybean meal as a good sulphate, carbon and nitrogen sources which influenced the maximum yield of this enzyme (137.69±4.57, 135.23±1.73 and 134.74±1.77, respectively) in comparison with the other sulphate, carbon and nitrogen sources.
A novel genetic approach for optimized biological sequence alignment  [PDF]
Gautam Garai, Biswanath Chowdhury
Journal of Biophysical Chemistry (JBPC) , 2012, DOI: 10.4236/jbpc.2012.32022
Abstract: Biological sequence alignment is one of the most important problems in computational biology. The objective of the alignment process is to maximize the alignment score between two given sequences of varying or equal length. The alignment score of two sequences is calculated based on matches, mismatches and gaps in the alignment. We have proposed a new genetic approach for finding optimized match between two DNA or protein sequences. The process is compared with two well known relevant sequence alignment techniques.
Preparation and Evaluation of Rapidly Disintegrating Fast Release Tablet of Diazepam-Hydroxypropyl-β-Cyclodextrin Inclusion Complex  [PDF]
Tapan Kumar Giri, Biswanath Sa
Pharmacology & Pharmacy (PP) , 2010, DOI: 10.4236/pp.2010.11003
Abstract: This study was undertaken to develop tablets of diazepam-hydroxypropyl-β-cyclodextrin inclusion complex that disintegrate within 3 minutes and release 85% of drug within 30 minutes to provide rapid action of the drug through oro-mucosal route. Formation of inclusion complex was verified using X-ray diffraction and differential scanning calorimetric studies. Enhanced of aqueous solubility, as evident from phase solubility study, and dissolution of the drug were related with the formation of inclusion complex. Among the various formulations, tablet containing inclusion complex of drug/hydroxypropyl-β-cyclodextrin in a molar ratio of 1:2, and a combination of microcrystalline cellulose/lactose in a ratio of 4:1 disintegrated in 13 seconds and released 85% drug within 9 minutes. Addition of 10% w/w polyvinyl pyrrolidone in the tablet formulation further enhanced the drug release. Accelerated stability study indicated that mean dissolution time of the drug from the tablet did not change significantly within 6 months.
Undergraduate medical education in India and scope of pediatric surgery teaching
Mukhopadhyay Biswanath
Journal of Indian Association of Pediatric Surgeons , 2009,
Iso-curvature fluctuations through axion trapping by cosmic string wakes
Biswanath Layek
Physics , 2005, DOI: 10.1103/PhysRevD.71.063527
Abstract: We consider wake-like density fluctuations produced by cosmic strings at the quark-hadron transition in the early universe. We show that low momentum axions which are produced through the radiation from the axionic string at an earlier stage, may get trapped inside these wakes due to delayed hadronization in these overdense regions. As the interfaces, bordering the wakes, collapse, the axions pick-up momentum from the walls and finally leave the wake regions. These axions thus can produce large scale iso-curvature fluctuations. We have calculated the detailed profile of these axionic density fluctuations and discuss its astrophysical consequences.
Effects of Slot Loading on Microstrip Patch Antennas
Sunandan Bhunia
International Journal of Wired and Wireless Communications , 2012,
Abstract: This study presents the theoretical and experimental investigation on the effect of rectangular slot on resonance frequency, return loss and band widths of a novel, single layer, single probe-fed rectangular microstrip patch antenna. The novel design is achieved by cutting the rectangular slots at the one radiating edge of the patch antenna. Compared to the conventional rectangular patch antenna, slot loading antennas give the better performance in resonant frequency, return loss in the cost of bandwidths. Resonance frequency reduction has also been achieved up to 36% as well as size reduction of more than 60%.
Polymethylmethacrylate Coated Alginate Matrix Microcapsules for Controlled Release of Diclofenac Sodium  [PDF]
Tapas Pal, Shubhajit Paul, Biswanath Sa
Pharmacology & Pharmacy (PP) , 2011, DOI: 10.4236/pp.2011.22007
Abstract: Polymethylmethacrylate (PMMA) coated microcapsules of diclofenac sodium (DFS) were prepared by a modified wa-ter-in-oil-in-water (W1/O/W2) emulsion solvent evaporation method using sodium alginate (SAL) as a matrix material in the internal aqueous phase (W1).Their performance with respect to controlled release of the drug in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) were evaluated, and compared with non-matrix microcapsules prepared by the conventional W1/O/W2 emulsion solvent evaporation method. Scanning electron micrographs (SEM) revealed that all the microcapsules were discrete and spherical in shape; however, the surface porosity of the matrix microcap-sules appeared to be less than that of the non-matrix microcapsules. In case of non-matrix microcapsules, an increase in the volume of water in W1 phase resulted in decrease in the drug entrapment efficiency (DEE) along with increase in release of the drug in both SGF and SIF. While in case of matrix microcapsules increase in the amount of SAL in W1 phase and concentration of the coating polymer in organic phase led to increase in DEE of the matrix microcapsules and considerable decrease in the drug release in both SGF and SIF. No interaction between the drug and any of the polymers used to prepare microcapsules was evident from Fourier transform infra-red (FTIR) analysis. The matrix microcapsules prepared using higher concentration of SAL and PMMA released the drug following zero order or Case-II transport model. The matrix microcapsules appeared to be suitable for releasing lesser amounts of DFS in SGF and providing extended release in SIF.
New Approaches for Selective Aes Towards Tackling Error Propagation Effect of Aes
Chandan T. Bhunia
Asian Journal of Information Technology , 2012,
Abstract: Advanced Encryption Standard (AES) has been developed to replace the Data Encryption Standard(DES). AES suffers from a major limitation of error propagation on its encryption process. To tackle this limitation two approaches are used: Redundancy based Technique and Byte Based Parity Technique. Redundancy based technique has some important advantages over parity based technique but at the cost of higher level of processing and lowering encryption speed. In this study we report the application of selective AES in redundancy based technique. Our technique gives better results in terms of higher throughput and higher speed of encryption over the conventional technique.
Compact Broad Band Dual Frequency Slot Loaded Microstrip Patch Antenna with Defecting Ground Plane for Wi-MAX and WLAN
Avisankar Roy,Sunandan Bhunia
International Journal of Soft Computing & Engineering , 2012,
Abstract: A dual frequency, compact microstrip patch antenna with enhanced bandwidth is presented in this paper. Microstrip antenna with bandwidth of 31% is also been designed for Wi-MAX application by defecting the ground plane The single layered antenna has been designed to resonate in dual frequencies for Wi-MAX and WLAN with enhanced bandwidth of more than 12%. Microstrip patch antenna with inset feed is simulated with the method of Moment based standard software.
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