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Search Results: 1 - 10 of 25081 matches for " Bing Qin1 "
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MicroRNA let-7c inhibits Bcl-xl expression and regulates ox-LDL-induced endothelial apoptosis
Bing Qin1, Bo Xiao1, Desheng Liang2, Ye Li1, Ting Jiang1 & Huan Yang1,*
BMB Reports , 2012,
Abstract: Endothelial cells (ECs) apoptosis induced by oxidizedlow-density lipoprotein (ox-LDL) is thought to play a criticalrole in atherosclerosis. MicroRNAs (miRNAs) are a class ofnoncoding RNAs that posttranscriptionally regulate the expressionof genes involved in diverse cell functions, includingdifferentiation, growth, proliferation, and apoptosis. MiRNAlet-7 family is known to be involved in the regulation of cellapoptosis. However, the function of let-7 in ox-LDL inducedECs apoptosis and atherosclerosis is still unknown. Here, weshow that let-7c expression was markedly up-regulated inox-LDL induced apoptotic human umbilical cord veinendothelial cells (HUVECs). Let-7c over-expression enhancedapoptosis in ECs whereas inhibition of let-7c could partlyalleviate apoptotic cell death mediated by ox-LDL. Searchingfor how let-7c affected apoptosis, we discovered that antiapoptoticprotein Bcl-xl was a direct target of let-7c in ECs. Ourdata suggest that let-7c contributes to endothelial apoptosisthrough suppression of Bcl-xl.
Blank Holder Force Control System Driven by Servo-Motor  [PDF]
Siji Qin, Li Yang, Bing Yang
Intelligent Control and Automation (ICA) , 2011, DOI: 10.4236/ica.2011.24051
Abstract: Blank holder force (BHF) control is used to prevent wrinkles of sheet metal in deep drawing process. Based on a novel conception of BHF control technique driven by servo-motor, a new BHF device with six-bar linkage mechanism has been designed and manufactured. Whole control system of the new BHF technique was developed, and the basic structure of the hardware configuration of the system was given. Software analysis, implementation and division of the functional modules have been done. Also, the control software in data acquisition and processing module has been developed in the relevant technology of the BHF control system for the requirements of real-time, stability and accuracy. By the new BHF device combined with the hardware and the software system, the BHF can be regulated accurately variation with the predefined BHF profile in deep drawing process.
Quantitative Analysis of ATP Sulfurylase and Selenocysteine Methyltransferase Gene Expression in Different Organs of Tea Plant (Camellia sinensis)  [PDF]
Shaoqiang Tao, Juan Li, Xungang Gu, Yanan Wang, Qiang Xia, Bing Qin, Lin Zhu
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.31004
Abstract: Tea plant (Camellia sinensis) has unique biological features for the study of cellular and molecular mechanisms, an evergreen broad-leaved woody plant which can accumulate selenium in soil abundant of Selenium. Expression of the genes related to Selenium (Se) metabolism is an adaptation to the soil environment for a long period. The purpose of the present study was to explore if there exist differences of expression about these genes in tea plant between growing in Selenium-abundant and normal soil. A quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) assay was done for quantification of ATP sulfurylase (APS) and selenocysteine methyltransferase (SMT) mRNA normalized to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene in tea plant. Young leaves, mature leaves and tender roots from tea plants growing in soil abundant of Selenium were respectively obtained from Shitai County, Anhui Province, and also the relevant materials of the selenium un-enriched tea plant planted at agricultural garden of Ahui Agriculture University were taken as control for real-time PCR analysis. The results showed that APS1, APS2 and SMT expression levels for either young or mature leaves in selenium-enriched tea plant were lower than that in ordinary (selenium un-enriched) tea plant. In contrast, the APS1, APS2 and SMT expression level of roots in selenium-enriched tea plant were all higher than that in ordinary tea plant. APS1 gene expression level of roots in selenium-enriched tea plant was about 1.6 times higher than that in the ordinary tea plant, APS2 gene expression level was about 4.8-fold higher than that in the ordinary tea plant, SMT gene expression level was about 3.3 times higher than that in the ordinary tea plant. Among various tissues of selenium-enriched tea plant, APS1 gene relative expression level of young leaves was similar to or slightly higher than mature leaves, and the one of roots was the lowest among them; APS2 gene relative expression level of young leaves was similar to or slightly higher than the roots, and the one of mature leaves was the lowest among them; SMT gene relative expression level of young leaves was similar to or slightly higher than mature leaves, and the one of roots was the highest among them. Our results suggest that there existed correlation between selenium and expression levels of these genes.
Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats
Xin-bing SUI,Qin ZHANG
Zhong Xi Yi Jie He Xue Bao , 2007,
Abstract: Objective: To observe the effects of salvianolate on rats with postoperative intestinal adhesion and to explore the prevention mechanism.Methods: Forty SD male rats with intestinal adhesion were randomly divided into four groups: untreated group, low-dose salvianolate-treated group (12 mg/kg), medium-dose salvianolate-treated group (24 mg/kg) and high-dose salvianolate-treated group (48 mg/kg), with another ten SD male rats as normal control. Intraperitoneal injection of glucose was administered to the rats in the normal control group and the untreated group, and intraperitoneal injection of salvianolate was administered to the rats in the low-, medium- and high-dose salvianolate-treated groups. They were all treated for 8 days and once a day. On the eighth day after surgery the blood samples of each group were collected. Grades of intestinal adhesion were ranked by macroscopic observation. The adhesive tissues between viscera and belly wall were taken for pathological observation. The levels of interleukin-1β (IL-1β), interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) were determined by enzyme linked immunosorbent assay.Results: Salvianolate can significantly reduce the extent of postoperative intestinal adhesion, obviously decrease the levels of IL-1β, TNF-α and inhibit the hyperplasy of fibrous connective tissue. However, there was no significant impact on the level of IL-4.Conclusion: Salvianolate can reduce the extent of postoperative intestinal adhesion, decrease the expression of IL-1β and TNF-α and inhibit the hyperplasy of fibrous connective tissue. This may be the mechanism of salvianolate in preventing intestinal adhesion
Bilateral Thalamic Glioma –A Rare Tumour
Vivek Rauniyar,Bing Qin,Huan Yang
Journal of Nobel Medical College , 2014, DOI: 10.3126/jonmc.v3i1.10059
Abstract: We report a case of 48-year old woman who presented with dementia, progressive mental decline, personality change, paresis of the right lower extremity, and gait ataxia. Magnetic resonance imaging (MRI) T1-weighted image revealed bilateral thalamic swelling with homogenous low signal intensity and mild contrast enhancement with gadolinium. The T2- weighted image showed high intensity lesions in the bilateral thalamus, septum pellucidum and fornix. A CT-guided stereotactic biopsy of the lesion revealed fibrillary astrocytoma grade III as per WHO classification. Bilateral thalamic gliomas are very rare tumours and when present may resemble other benign diseases having similar clinical presentations DOI: http://dx.doi.org/10.3126/jonmc.v3i1.10059 ? Journal of Nobel Medical College Vol.3(1) 2014; 71-73
Comparative analysis of current and magnetic multipole graphical models  [PDF]
Shi-Qin Jiang, Lu Bing, Jia-Ming Dong, Ming Chi, Wei-Yuan Wang, Lei Zhang
Journal of Biomedical Science and Engineering (JBiSE) , 2009, DOI: 10.4236/jbise.2009.28088
Abstract: In recent year, a multipole graphical model, which is constructed by using individual MCG measurements based on the equivalent current dipole (ECD) or equivalent magnetic dipole (EMD) source model, has been developed with the aim of instead of the volume conductor model in the inverse solution of cardiac source estimation. In this paper, two graphical models known as the double magnetic dipole source model (DMD) and the dual current dipole source model (DCD) are introduced. The simulation results and the comparison of two evaluation criteria, i.e. average GOF (Goodness of Fit) and average RMSE (Root Mean Square Error), indicated that both multipole graphical models can provide a good representation of dynamic magnetic field from the noninvasively detected MCG- recordings, even when the heart is of the dilation. The time-averaged sources localization error and the RMSE for both models are demonstrated, and the characteristic of two multipole models is discussed.
Role of Asp37 in metal-binding and conformational change of ciliate Euplotes octocarinatus centrin  [PDF]
Wen Liu, Lian Duan, Bing Zhao, Ya-Qin Zhao, Ai-Hua Liang, Bin-Sheng Yang
Health (Health) , 2010, DOI: 10.4236/health.2010.23037
Abstract: Centrin is a member of the EF-hand super family that plays critical role in the centrosome duplication and separation. To investigate the role of Asp37 in the process of metal-binding and conformational change of ciliate Euplotes octocarinatus centrin (EoCen), the mutant D37K, in which aspartic acid 37 had been replaced by lysine, was first obtained by the site-directed mutagenesis. Then 2-p-toluidinylnaphthalene-6- sulfonate (TNS) was used as a fluorescence probe to detect the conformational change of the protein. The results show that the metal- binding capability of the site I of EoCen was lost by the mutation of Asp37→Lys. In comparison the Tb3+-saturated EoCen, the hydrophobic surface of D37K, which is exposed by the binding of Tb3+, has shrunk sharply, suggesting that Asp37 plays an important role in maintaining the proper conformation of EoCen in the presence of Tb3+. Meanwhile, the conditional binding constants of TNS with Tb3+-loaded EoCen and D37K were obtained, K(Tb3+-EoCen-TNS)=(7.38 ±0.25)×105 M-1 and K(Tb3+-D37K-TNS)=(1.16± 0.05)×106 M-1.
Analysis of the Bacterial Communities in Lime Concretion Black Soil upon the Incorporation of Crop Residues  [PDF]
Shao-Qiang Tao, Qiang Xia, Lin Zhu, Jing-jing Chen, Ya- Nan Wang, Bing Qin
Open Journal of Soil Science (OJSS) , 2012, DOI: 10.4236/ojss.2012.23037
Abstract: To analyze the bacterial communities in lime concretion black soil upon the incorporation of crop residues for two years in wheat-maize system, total DNA was directly extracted and PCR-amplified with the F357GC and R518 primers targeting the 16S rRNA genes of V3 region. The amplified fragments were analyzed by perpendicular DGGE. Analyzing of species richness index S and Shannon diversity index H revealed that there was a high diversity of soil bacterial community compositions among all treatments after incorporation of crop residues and fertilizing under field conditions. Eleven DGGE bands recovered were re-amplified, sequenced. Phylogenetic analysis of the representative DGGE fingerprints identified four groups of the prokaryotic communities in the soil by returning wheat residues and fertilizing under field conditions. The bacterial communities belonged to gamma proteobacterium, Cupriavidus sp, halophilic eubacterium, Acidobacterium sp, Sorangium sp, delta proteobacterium, Streptococcus sp and Streptococcus agalactiae were main bacterial communities. Principal Component Analysis (PCA) showed that there were the differences in DNA profiles among the six treatments. It showed that wheat residue returning, maize residue returning and fertilizing all can improve bacterial diversity in varying degrees. As far as improvement of bacterial diversity was concerned, wheat residue returning was higher than fertilizing, and fertilizing higher than maize residue returning.
In Silico Analysis of a MRP Transporter Gene Reveals Its Possible Role in Anthocyanins or Flavonoids Transport in Oryze sativa  [PDF]
Qin-Long Zhu, Xian-Rong Xie, Jia Zhang, Ge Xiang, Yun Li, Hai-Bing Wu
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.43072
Abstract:

There are many studies on enzymatic pathways of anthocyanin biosynthesis, but little is known about the anthocyanins transport in Oryze sativa. In silico analysis, the OsMRP15 (LOC_Os06g06440), an orthologous gene of mazie anthocyanin transporter ZmMRP3, has been identified in rice. The OsMRP15 contained a 4425bp open reading frame (ORF) encoding a 1475 amino acid protein, belonging to a MRP subfamily of ABC transporters, and has a high sequence identity, very similar protein structure, and the same arrangement of domains to ZmMRP3, but the genomic structure of OsMRP15 was significant difference with ZmMRP3. The prediction promoter of OsMRP15 has many presumed anthocyanin regulatory sites. The phylogenetic analysis of MRPs in rice, mazie and Arabidopsis showed that OsMRP15 and ZmMRP3 belonged to the same subbranch. The expression pattern indicated that OsMRP15 was co-expression with two anthocyanin transcription factors. These analysis results implied that as an ortholog of ZmMRP3, the function of OsMRP15 was possibly as a membrane-bound transporter required for vacuolar uptake of anthocyanins in rice.

Bis(μ-2-phenylquinoline-4-carboxylato)-κ3O,O′:O;κ3O:O,O′-bis[(2,2′-bipyridine-κ2N,N′)(2-phenylquinoline-4-carboxylato-κ2O,O′)cadmium(II)]
Mei-Qin Zha,Xing Li,Yue Bing
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536810049640
Abstract: The neutral binuclear title complex, [Cd2(C16H10NO2)4(C10H8N2)2], is centrosymmetric, with the inversion center generating the central (μ-O)2Cd2 bridge. The CdII ion is in a strongly distorted CdN2O5 pentagonal-bipyramidal geometry, defined by two N atoms from one 2,2′-bipyridine ligand and five O atoms from three 2-phenylquinoline-4-carboxylate ligands, one monodentate, two bidentate. Weak intermolecular π–π interactions [centroid–centroid distance = 3.712 (3) ] help to establish the packing of the structure.
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