oalib

OALib Journal期刊

ISSN: 2333-9721

费用:99美元

投稿

匹配条件: “Bentancor” ,找到相关结果约18条。
列表显示的所有文章,均可免费获取
第1页/共18条
每页显示
DIOS, dioses y diositos: Una lectura de la primera parte del Popol Vuh en comparación con los primeros capítulos del Génesis
Bentancor,Milton Hernán;
Enfoques , 2012,
Abstract: summary in the following paper we want to observe, from a literary point of view, the story of creation in the popol vuh told by the mayas - quiches indigenous and compare it with the biblical story of genesis. finding the commonalities and differences between both texts will approach us to the idea of divinity that they present. besides, we will be able to see the cosmogony and the literary stiles used in each case. as there are more differences (in quantity and in depth) than similarities, this analysis will let us go deeper in the idea of perfection and organization of the biblical god, as opposed to the almost human imperfection of the pre - columbian american gods.
Erwinia carotovora elicitors and Botrytis cinerea activate defense responses in Physcomitrella patens
Inés Ponce de León, Juan Oliver, Alexandra Castro, Carina Gaggero, Marcel Bentancor, Sabina Vidal
BMC Plant Biology , 2007, DOI: 10.1186/1471-2229-7-52
Abstract: B. cinerea and E.c. carotovora were found to readily infect Physcomitrella gametophytic tissues and cause disease symptoms. Treatments with B. cinerea spores or cell-free culture filtrates from E.c. carotovoraSCC1 (CF(SCC1)), resulted in disease development with severe maceration of Physcomitrella tissues, while CF(SCC3193) produced only mild maceration. Although increased cell death was observed with either the CFs or B. cinerea, the occurrence of cytoplasmic shrinkage was only visible in Evans blue stained protonemal cells treated with CF(SCC1) or inoculated with B. cinerea. Most cells showing cytoplasmic shrinkage accumulated autofluorescent compounds and brown chloroplasts were evident in a high proportion of these cells. CF treatments and B. cinerea inoculation induced the expression of the defense-related genes: PR-1, PAL, CHS and LOX.B. cinerea and E.c. carotovora elicitors induce a defense response in Physcomitrella, as evidenced by enhanced expression of conserved plant defense-related genes. Since cytoplasmic shrinkage is the most common morphological change observed in plant PCD, and that harpins and B. cinerea induce this type of cell death in vascular plants, our results suggest that E.c. carotovora CFSCC1 containing HrpN and B. cinerea could also induce this type of cell death in Physcomitrella. Our studies thus establish Physcomitrella as an experimental host for investigation of plant-pathogen interactions and B. cinerea and elicitors of E.c. carotovora as promising tools for understanding the mechanisms involved in defense responses and in pathogen-mediated cell death in this simple land plant.Plants are continuously subjected to pathogen attack and respond by activating a range of defense mechanisms. Recognition of the pathogen or elicitors derived either from the pathogen or released from the plant cell wall is accompanied with the production of molecular signals including salicylic acid [1], jasmonic acid [2] and ethylene [3] that lead to the ind
Contaminación de carne molida con cepas de Escherichia coli shigatoxigénico (STEC) provenientes de comercios minoristas de San Martín, Buenos Aires, categorizados según nivel socioeconómico
Miccio,L; Rumi,M.V; Llorente,P; Bentancor,A.B;
InVet , 2011,
Abstract: shiga toxin-producing escherichia coli (stec) causes foodborne diseases, from mild diarrhea to hemolytic uremic syndrome which impact in argentina. many human infections were acquired from eating undercooked contaminated beef. the contamination of ground beef and their relation to socio-economic strata has not been studied. the aim of this study was to identify stec in meat samples and establish virulence profile, considering the socio-economic area of origin and persistence of contamination in local sales. it was selected 30% of the butcher shop of each socioeconomic level. it was analyzed 72 samples from 36 butcher shop of san martín, buenos aires, in two independent sampling using multiplex pcr for stx1/stx2 and immunocapture for o157 at screening. eleven strains were obtained from 26 suspect samples, 7% of the isolates were stec o157. the weighted proportion of contamination was higher in the middle and low strata. contamination from butcher shops was not persistent in both sampling. the degree of contamination by stec in meat and the virulence of isolates were high for the study area. in view of this it is necessary to implement programs which help to reduce risks in public health.
Factores de riesgo de infección por cepas de Escherichia coli shigatoxigénicas en gatos y perros
Bentancor,A; Agostini,A; Rumi,M.V; Degregorio,O.J;
InVet , 2008,
Abstract: hemolytic uremic syndrome caused by shiga toxin-producing escherichia coli, stec, is endemic in argentina. although the disease is classified as a food transmitted disease, personto- person infection has been postulated to have a high impact in our country. given the close relationship between pets and their owners, the pet-owner transmission route has been analyzed in urban centers. the aim of this work is to communicate the risk-factor analysis in pets that carry stec in buenos aires, argentina, providing useful information to evaluate the risk of transmission of this pathogen. rectal swabs were collected from 149 cats and 450 dogs from april 2005 to march 2006. a questionnaire was filled in for each animal. bacteria from samples were cultured and screened for shiga-toxin genes (stx) by pcr. stec isolated were recovered from stx-positive samples, in this study, all isolated strains were stx2+. of the 113 healthy cats sampled, 4 (2.7%) were stx+. of the 373 healthy dogs, 4 (1.1%) were stx+, while 1 of 29 (3.4%) dogs with diarrhea was infected. statistical analyses of 12 parameters revealed several riskfactors for the presence of stec: potentially contaminated food, age of the pets, frequent pet-topet contact and season. no significant differences between healthy or diarrheic pets concerning risks for carrying stec were observed.
Detección de la actividad de desoxiribonucleasa (DNasa) en cepas de Clostridium chauvoei
Carloni,G.H.; Bentancor,L.D.; De Torres,R.A.;
Revista argentina de microbiolog?-a , 2005,
Abstract: beta toxin of c. chauvoei has desoxiribonuclease (dnase) activity which is regarded as one of its virulence factors. the production of dnase was detected in strains isolated from bovines, using as controls c. chauvoei atcc 10092, and c. perfringens type a and c. septicum, both laboratory isolates. the enzyme activity was made evident on a dna substrate observing the macroscopic degradation. a simple methodology was developed using a commercial medium for dnase test, with the incorporation of sterile horse serum. each strain was streaked on the surface of the medium, incubated in anaerobic atmosphere at 37 °c for 48 hours. the plates were revealed with hcl 1n. the appearance of a clear and transparent zone around and under the microbial growing was considered a positive reaction. enzyme activity was detected in 10 of 12 strains and also in the controls. the serum addition to the commercial basal medium allows the optimum development of the microorganism showing the enzymatic digestion zone.
Actualización en el tratamiento del síndrome urémico hemolítico endémico: Patogénesis y tratamiento de la complicación sistémica más grave de las infecciones por Escherichia coli productor de toxina Shiga Update on the treatment of endemic hemolytic uremic syndrome: Pathogenesis and treatment of the most severe systemic complication of infections by Shiga toxin-producing Escherichia coli
Romina J. Fernández-Brando,Leticia V. Bentancor,Maria Pilar Mejías,Analía C. Panek
Medicina (Buenos Aires) , 2011,
Abstract: La forma típica o post-diarreica del síndrome urémico hemolítico (SUH) es la complicación más grave de las infecciones por cepas de Escherichia coli productoras de toxina Shiga (STEC). En la Argentina el SUH es un problema crítico de salud pública, ya que representa la principal causa de falla renal aguda en la infancia, la segunda causa de falla renal crónica, y aporta el 20% de los casos de transplante renal durante la infancia y la adolescencia. A pesar de los avances en el conocimiento de su patogénesis, el único tratamiento actual de los pacientes con SUH es de sostén, y no existen terapias específicas ni preventivas. En la presente revisión expondremos los conocimientos básicos de los mecanismos patogénicos y discutiremos los enfoques terapéuticos tradicionales e innovadores, con especial foco en la situación nacional y los aportes hechos por grupos de la Argentina. The typical form of hemolytic uremic syndrome (HUS) is the major complication of Shiga toxin-producing Escherichia coli (STEC) infections. HUS is a critical health problem in Argentina since it is the main cause of acute renal failure in children and the second cause of chronic renal failure, giving account for 20% of renal transplants in children and adolescents in our country. In spite of the extensive research in the field, the mainstay of treatment for patients with HUS is supportive therapy, and there are no specific therapies preventing or ameliorating the disease course. In this review, we present the current knowledge about pathogenic mechanisms and discuss traditional and innovative therapeutic approaches, with special focus in national status and contributions made by Argentinean groups.
Factores de riesgo de infección por cepas de Escherichia coli shigatoxigénicas en gatos y perros Risk factors for Shiga toxin-producing Escherichia coli infection in cats and dogs
A Bentancor,A Agostini,M.V Rumi,O.J Degregorio
InVet , 2008,
Abstract: El Síndrome Urémico Hemolítico causado por Escherichia coli shigatoxigénico, STEC, es endémico en Argentina. Si bien es una enfermedad de Transmisión Alimentaria, se postula que el contagio persona-persona tiene alto impacto en nuestro país. Dada la estrecha relación personasanimales de compa ía en los centros urbanos, esta ruta ha sido evaluada. El objetivo del trabajo es comunicar los factores de riesgo asociados a la portación-infección por STEC en animales de compa ía de Buenos Aires, Argentina, proporcionando información útil para evaluar el riesgo de transmisión de este patógeno. Se recolectaron hisopados rectales de 149 gatos y 450 perros desde abril de 2005 a marzo de 2006. Se elaboró una ficha epidemiológica para cada animal. Se realizó rastrillaje de los genes de Shigatoxina (stx) en los cultivos bacterianos por PCR. Todas las cepas aisladas en los cultivos stx+ fueron stx2+. De los 113 gatos clínicamente sanos evaluados, 4 (2,7%) fueron stx+. De los 373 perros clínicamente sanos 4 (1,1%) fueron stx+ mientras que de 29 perros con diarrea 1 fue positivo (3,4%). El análisis de 12 parámetros, evidenció algunos factores de riesgo de presencia de STEC: comida potencialmente contaminada, edad del animal, contacto frecuente con otras mascotas y estación del a o. No se determinaron diferencias significativas entre los animales clínicamente sanos o diarreicos respecto al riesgo de portación de cepas STEC. Hemolytic Uremic Syndrome caused by Shiga toxin-producing Escherichia coli, STEC, is endemic in Argentina. Although the disease is classified as a Food Transmitted Disease, personto- person infection has been postulated to have a high impact in our country. Given the close relationship between pets and their owners, the pet-owner transmission route has been analyzed in urban centers. The aim of this work is to communicate the risk-factor analysis in pets that carry STEC in Buenos Aires, Argentina, providing useful information to evaluate the risk of transmission of this pathogen. Rectal swabs were collected from 149 cats and 450 dogs from April 2005 to March 2006. A questionnaire was filled in for each animal. Bacteria from samples were cultured and screened for Shiga-toxin genes (stx) by PCR. STEC isolated were recovered from stx-positive samples, in this study, all isolated strains were stx2+. Of the 113 healthy cats sampled, 4 (2.7%) were stx+. Of the 373 healthy dogs, 4 (1.1%) were stx+, while 1 of 29 (3.4%) dogs with diarrhea was infected. Statistical analyses of 12 parameters revealed several riskfactors for the presence of STEC: potentially con
Contaminación de carne molida con cepas de Escherichia coli shigatoxigénico (STEC) provenientes de comercios minoristas de San Martín, Buenos Aires, categorizados según nivel socioeconómico Contamination of Shiga toxin-producing Escherichia coli (STEC) in fresh ground beef from butcher shops in San Martin, Buenos Aires Province, among different socioeconomic strata
L Miccio,M.V Rumi,P Llorente,A.B Bentancor
InVet , 2011,
Abstract: Escherichia coli shigatoxigénico (STEC) produce enfermedades de transmisión alimentaria, desde diarreas leves a Síndrome Urémico Hemolítico, enfermedad de impacto en Argentina. Dentro de los alimentos implicados se destaca la carne bovina insuficientemente cocida. La contaminación de la carne molida y su relación con los estratos socioeconómicos no ha sido estudiada. El objetivo de este trabajo fue identificar en muestras de carne cepas STEC y establecer su perfil de virulencia, considerando la zona socioeconómica de procedencia y la persistencia de la contaminación en el local de venta. Se seleccionó el 30% de las bocas de expendio habilitadas en cada nivel socioeconómico. Se analizaron 72 muestras provenientes de 36 carnicerías de San Martín, Buenos Aires, en dos muestreos independientes utilizando PCR múltiple para stx1/stx2 e inmunocaptura para O157 al tamizaje. Se obtuvieron 11 cepas de 26 muestras sospechosas, 7% de los aislamientos fueron STEC O157. La proporción en la contaminación ponderada fue mayor en las zonas media y baja. No se comprobó persistencia. El grado de contaminación por STEC en carne para el área estudiada fue elevado y las cepas aisladas fueron altamente virulentas. En consideración a ello es necesario implementar programas de capacitación y control para reducir los riesgos para la salud pública. Shiga toxin-producing Escherichia coli (STEC) causes foodborne diseases, from mild diarrhea to hemolytic uremic syndrome which impact in Argentina. Many human infections were acquired from eating undercooked contaminated beef. The contamination of ground beef and their relation to socio-economic strata has not been studied. The aim of this study was to identify STEC in meat samples and establish virulence profile, considering the socio-economic area of origin and persistence of contamination in local sales. It was selected 30% of the butcher shop of each socioeconomic level. It was analyzed 72 samples from 36 butcher shop of San Martín, Buenos Aires, in two independent sampling using multiplex PCR for stx1/stx2 and immunocapture for O157 at screening. Eleven strains were obtained from 26 suspect samples, 7% of the isolates were STEC O157. The weighted proportion of contamination was higher in the middle and low strata. Contamination from butcher shops was not persistent in both sampling. The degree of contamination by STEC in meat and the virulence of isolates were high for the study area. In view of this it is necessary to implement programs which help to reduce risks in public health.
Detección de la actividad de desoxiribonucleasa (DNasa) en cepas de Clostridium chauvoei Desoxiribonuclease activity detection in Clostridium chauvoei strains
G.H. Carloni,L.D. Bentancor,R.A. De Torres
Revista argentina de microbiolog?-a , 2005,
Abstract: La toxina beta de Clostridium chauvoei posee actividad de desoxiribonucleasa (DNasa) y se considera uno de sus principales factores de virulencia. Se detectó su producción a partir de cepas provenientes de aislamientos bovinos, empleando como controles C. chauvoei American Type Culture Collection (ATCC) 10092 y C. septicum y C. perfringens tipo A, ambos aislamientos de laboratorio. La actividad enzimática se evidenció sobre un sustrato sólido con el agregado de DNA y verificando macroscópicamente su degradación. Se desarrolló una metodología sencilla empleando agar comercial para ensayo de DNasa, con agregado de suero equino estéril. Cada cepa se sembró sobre la superficie del medio de cultivo, incubando en atmósfera anaerobia hasta 48 horas a 37 °C. Los cultivos se sometieron a la acción de ácido clorhídrico (HCl) 1N. La aparición de una zona clara y transparente alrededor y por debajo del desarrollo microbiano se consideró reacción positiva. La actividad enzimática se detectó en 10 de 12 cepas estudiadas y en los controles. El agregado de suero al medio base comercial permitió el desarrollo óptimo de los microorganismos evidenciando con claridad la zona de digestión enzimática. Beta toxin of C. chauvoei has desoxiribonuclease (DNase) activity which is regarded as one of its virulence factors. The production of DNase was detected in strains isolated from bovines, using as controls C. chauvoei ATCC 10092, and C. perfringens Type A and C. septicum, both laboratory isolates. The enzyme activity was made evident on a DNA substrate observing the macroscopic degradation. A simple methodology was developed using a commercial medium for DNase test, with the incorporation of sterile horse serum. Each strain was streaked on the surface of the medium, incubated in anaerobic atmosphere at 37 °C for 48 hours. The plates were revealed with HCl 1N. The appearance of a clear and transparent zone around and under the microbial growing was considered a positive reaction. Enzyme activity was detected in 10 of 12 strains and also in the controls. The serum addition to the commercial basal medium allows the optimum development of the microorganism showing the enzymatic digestion zone.
Characterization of non -Shiga toxin- producing Escherichia coli O157 strains isolated from dogs Caracterización de cepas de Escherichia coli O157 no productoras de toxina Shiga aisladas de perros
A. Bentancor,D. A. Vilte,M. V. Rumi,C. C. Carbonari
Revista argentina de microbiolog?-a , 2010,
Abstract: Shiga toxin-negative Escherichia coli O157 strains of various H types have been associated with diarrhea in children and are considered potentially pathogenic for humans. In this study, we describe non-Shiga toxin-producing E. coli O157 E. coli strains previously obtained from dogs in Argentina. Different E. coli phylogenetic lineages corresponding to flagellar types H16, H29 and H45 were identified. E. coli serotypes O157:H16 and O157:H45 contained intimin subtypes e and a1, respectively. Serotype O157:H45 carried the bfp gene encoding the bundle-forming pilus. Localized adherence-like patterns to HEp-2 cells were observed in O157:H16 strains, while O157:H45 adhered in a typical localized pattern. A total of eight different XbaI-pulse field electrophoresis patterns with more than 74 % similarity were identified among the nine E. coli O157:H16 strains. Our data emphasized the fact that dogs may harbor human pathogenic E. coli O157 which do not correspond to Shiga toxin-producing strains and whose potential human health hazard should not be underestimated. Cepas de E. coli O157 no productoras de toxina Shiga (Stx) que presentan diversos antígenos flagelares han sido aisladas de ni os con diarrea y se consideran potencialmente patógenas para humanos. En el presente trabajo se describen cepas Stx-negativas de E. coli O157 de distintos tipos flagelares previamente aisladas de perros en Argentina. Los tipos flagelares H16, H29 y H45 correspondieron a diferentes grupos filogenéticos. Los serotipos O157:H16 y O157:H45 presentaron los subtipos de intimina e y a1, respectivamente. En el serotipo O157:H45 se detectó la presencia del gen bfp, codificante de la fimbria bundle-forming pili. El patrón de adherencia en células HEp-2 correspondió al tipo simil a localizada para E. coli O157:H16, mientras que O157:H45 mostró adherencia localizada típica. Dentro de las 9 cepas de E. coli O157:H16 estudiadas se detectaron 8 patrones de XbaI-PFGE con más del 74% de similitud. Nuestros datos confirman que los perros pueden ser portadores de E. coli O157 patógenas no productoras de Stx, las que representan un riesgo para la salud pública que no debe ser subestimado.
第1页/共18条
每页显示


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.