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Search Results: 1 - 10 of 39145 matches for " Beatriz Martínez Salgueiro "
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Lepto tek lateral flow: un método para el diagnóstico rápido de la leptospirosis humana en Cuba Lepto tek lateral flow: a method for the rapid diagnosis of human leptospirosis in Cuba
Beatriz Martínez Salgueiro,Ana Margarita Obregón Fuentes,Carmen Fernández Molina,José Rodríguez
Revista Cubana de Higiene y Epidemiología , 2005,
Abstract: La técnica de Lepto tek lateral flow (LF) en el diagnóstico serológico y rápido de casos graves con sospecha clínica y epidemiológica de leptospirosis humana se introdujo y se aplicó en el Laboratorio Nacional de Referencia de Leptospiras (LNRL). Se emplearon las técnicas convencionales de laboratorio (microaglutinación [MAT] y hemaglutinación indirecta [HA]) en la confirmación de los casos en estudio. Se estudiaron 40 muestras de sueros correspondientes a 33 pacientes sospechosos de leptospirosis humana, de las cuales se obtuvo positividad en 21 de ellas mediante LF, que representa un 52,5 %. Al aplicar comparativamente las técnicas de LF y HA en 26 monosueros y 7 pares de sueros, se obtuvieron 11 y 5 casos positivos respectivamente. La coincidencia entre ambas fue de un 73 % al estudiar los monosueros y del 71,0 al estudiar los pares de sueros. Al aplicar la MAT en 9 casos sospechosos, resultaron 7 positivos. Los serogrupos más frecuentes encontrados fueron: icterohaemorrhagiae, y canicola. Se detectaron solo 5 casos positivos por las 3 técnicas aplicadas. La coincidencia entre las técnicas de MAT y LF fue del 89 % y la de HA y la MAT del 100,0. La aplicación de la MAT y la HA ofreció confiabilidad en los resultados obtenidos y se evidenció la gran sensibilidad del LF. Se demostró la importancia del uso de sueros pareados para el diagnóstico certero de esta enfermedad. The Lepto Tek Lateral Flow technique (LF) in the serological and rapid diagnosis of severe cases with clinical and epidemiological suspicion of human leptospirosis was introduced in and applied by the National Laboratory of Leptospirosis Reference (NLLR). Conventional lab techniques (microagglutination MAT and indirect hemagglutination HA ) were used in the confirmation of cases under study, 40 serum samples corresponding to 33 patients suspected of human leptospirosis were studied. Of them, 21 samples proved to be positive by LF, accounting for 52.5 %. On comparatively applying the LF and HA techniques to 26 monosera and 7 pairs of serum, 11 and 5 positive cases were obtaind, respectively. The coincidence between them was 73 % on studying monosera and 71.0 on studying the pairs of serum. On applying MAT to 9 suspected cases, 7 of them yielded positive. The most frequently found serogroups were: Icterohaemorrhagiae and Canicola. Only 5 positive cases were detected by the 3 applied techniques. The coincidence between MAT and LF techniques was 89 % and that between HA and MAT was 100.0. The application of MAT and HA offered reliability to the obtained results and it was evidenced the high
Falsos biológicos positivos por VDRL en el diagnóstico serológico de la sífilis Biological false positive VDRL results in the serological diagnosis of syphilis
Islay Rodríguez González,Carmen Fernández Molina,María Beatriz Martínez Salgueiro
Revista Cubana de Medicina Tropical , 2006,
Abstract:
Lepto tek lateral flow: un método para el diagnóstico rápido de la leptospirosis humana en Cuba
Martínez Salgueiro,Beatriz; Obregón Fuentes,Ana Margarita; Fernández Molina,Carmen; Rodríguez,José; Rodríguez,Islay;
Revista Cubana de Higiene y Epidemiolog?-a , 2005,
Abstract: the lepto tek lateral flow technique (lf) in the serological and rapid diagnosis of severe cases with clinical and epidemiological suspicion of human leptospirosis was introduced in and applied by the national laboratory of leptospirosis reference (nllr). conventional lab techniques (microagglutination mat and indirect hemagglutination ha ) were used in the confirmation of cases under study, 40 serum samples corresponding to 33 patients suspected of human leptospirosis were studied. of them, 21 samples proved to be positive by lf, accounting for 52.5 %. on comparatively applying the lf and ha techniques to 26 monosera and 7 pairs of serum, 11 and 5 positive cases were obtaind, respectively. the coincidence between them was 73 % on studying monosera and 71.0 on studying the pairs of serum. on applying mat to 9 suspected cases, 7 of them yielded positive. the most frequently found serogroups were: icterohaemorrhagiae and canicola. only 5 positive cases were detected by the 3 applied techniques. the coincidence between mat and lf techniques was 89 % and that between ha and mat was 100.0. the application of mat and ha offered reliability to the obtained results and it was evidenced the high sensitivity of lf. the importance of the use of matched sera for the accurate diagnosis of this disease was demonstrated.
GENDER, SOCIAL PARTICIPATION, ENVIRONMENTAL AWARENESS AND REMEDIATION IN NATURAL DISASTERS, IN AN INDIGENOUS TOWN, CUETZALAN, PUEBLA
Beatriz Martínez-Corona
Ra Ximhai , 2012,
Abstract: In this work, it discusses the importance of gender equal perspective in the prevention and mitigation of natural disasters. We asked about the perceptions and reactions differential by gender about natural disasters and the effects of environmental changes in the territory of members of local organizations in Cuetzalan, Puebla. In particular nahuatl women organized in the Masehualsiuamej Mosenyolchiauanij, who developed remedial action from the effects of natural disaster in 1999. We asked about the perception of people about their production practices, natural disaster and involvement strategies followed by local social organizations to external projects. The methodology used in-depth interviews were conducted, participant observation and documental review. It also identified recent local actions denoting the generation of a model of social participation of local co-management, where members of civil society organizations, local authorities and indigenous social organizations seeking to implement an ecological management of their territory.
Some experiences related to innovation methodologies within the university classroom
Beatriz Amante García,María Martínez Martínez
Journal of Technology and Science Education , 2012, DOI: 10.3926/jotse.43
Abstract: In this second year of our Journal JOTSE our main challenge is to publish experiences related to innovation methodologies within the university classroom. Thus, allowing for the implementation and/or evaluation of competences throughout students’ learning process and, especially, in the scientific and technological fields. We understand competences as the combination of knowledge, skills and attitudes necessary to perform a task efficiently. Thereby, demonstrating abilities in action and developing them through activities that integrate all these aspects. In the area of higher studies in the scientific and/or technological fields it is rather common that the methodologies developed have a very practical component and, in addition, they are closely linked to the professional career our students are being trained for. Particularly, in the last academic years and at the end of their studies it is when students attend more applied subjects, such as Projects in the case of Engineering Studies that together with the Degree’s Final Project (PFC) allow to integrate a wide range of generic or cross-curricular competences and specific ones within the field. These types of subjects have shown to be very efficient to make students become closer to the professional reality that they will face at the end of their studies and where they will have to provide a solution for problematic situations or to meet the needs nowadays society demands. (Dochy et al. (2003), Prince (2004),Prince and Felder (2006)) Furthermore, with the incorporation to Bologna process, targeting a more active role of students during their learning process, it is even more relevant that students face real problems from the very beginning of their studies so that they gradually acquire generic competences, which are vital for their training both as individuals and as professionals in our present society. It is within this context that Problem-Based Learning (PBL) has proved its usefulness to reach such an objective. In this sense, several authors have shown that PBL can be successfully implemented in Engineering studies and as early as in the first year (Del Canto, 2011) by integrating teamwork among other competences.
Teaching innovation and its role crafting the classroom of today and the future
Beatriz Amante García,María Martínez Martínez
Journal of Technology and Science Education , 2011, DOI: 10.3926/jotse.18
Abstract: The Journal of Technology and Science Education (JOTSE) has been created as a contribution to the development and improvement of scientific and technological education by constituting a common space to share experiences to all those who, somehow, are involved in the teaching and learning processes of engineering studies, in all its modalities.
Towards methodological improvement in the Spanish studies
Beatriz Amante García,María Martínez Martínez
Journal of Technology and Science Education , 2012, DOI: 10.3926/jotse.53
Abstract: The European Higher Education Area (EHEA) has triggered many changes in the new degrees in Spanish universities, mainly in terms of methodology and assessment. However, in order to make such changes a success it is essential to have coordination within the teaching staff as well as active methodologies in use, which enhance and encourage students’ participation in all the activities carried out in the classroom. Most of all, when dealing with formative and summative evaluation, in which students become the ones responsible for their own learning process (López-Pastor, 2009; Torre, 2008). In this second issue of JOTSE we have included several teaching innovation experiences related to the above mentioned methodological and assessment changes.
Proposal for improve your evaluation skills
Beatriz Amante García,María Martínez Martínez
Journal of Technology and Science Education , 2013, DOI: 10.3926/jotse.73
Abstract: In this third year of our Journal JOTSE our main challenge is to publish experiences related to evaluation of competences and new strategies within the university classroom. As we have mentioned in the past, we understand competences as the combination of knowledge, skills and attitudes necessary to perform a task efficiently. One of the key skills for the professional world of engineers is ethics. In general, professional ethics instruction is introduced to students (around the world) using a case study approach.
Good learning practices in the field of science and technology
Beatriz Amante García,María Martínez Martínez
Journal of Technology and Science Education , 2011, DOI: 10.3926/jotse.27
Abstract: The European Higher Education Area (EHEA) scenario offers a new framework in which the role of universities can be rethought, regardless of the field of new degrees and postgraduate Studies involved. Therefore, the roles of teachers and students might differ significantly as the student would be obliged to request teacher’s guidance in order to acquire not only knowledge, which could be easily obtained elsewhere (books, internet etc. ), but also personal skills and, especially, those related to their future professional career. By the term “Good learning practices” we refer to those activities asociated with professorship itself ,such as those of facilitating and guiding the student learning process, or rather, those activities which are aimed at the student′s thorough learning of specific (related to the field of study ) and generic skills. It is now a common occurrence to describe the students of a given classroom as being little motivated and as having great interest in passing but not in actually learning. This fact is quite concerning, as it suggests that the student sees the university as a mere transaction by which they can obtain a degree, certifying that they are apt for the professional world, where they consider the “real” learning will take place. A good classroom environment is essential for the generation of teaching-learning processes. It is precisely because of this that we are able to raise the issue of effective practices among teachers who foster a suitable classroom dynamics facilitating, then, the targeted learning experience. Within this context, there are some authors who discuss good practices by professors especially concerned on how to perform assessment and feedback to enhance student’s learning activity. Thus, providing them with a deep and lasting impact. Generally speaking, the activities in question are those which enable the student to execute a learning process that will continue throughout their professional and personal lives. In the present issue we have selected a number of articles related to good practices taking place in the context of new studies plan implementation (EHEA ) in several Spanish Universities. In the first paper: Reflections and conclusions on the work developed by the group of teaching innovation in electronics at the university of Valladolid ,they present the main conclusions and results obtained over the three last academic years in the Electronics and Automatic Industrial Engineering degree. As a result of cooperative work of the group, an improvement in activity coordination and provis
Isolation of L. interrogans Serovar Pomona in 14 Human Cases and an African Lion, All with Chronic Leptospirosis  [PDF]
Beatriz Rivas-Sánchez, Oscar Velasco-Castrejón, Jesús Jimenez-Martínez
Open Journal of Medical Microbiology (OJMM) , 2016, DOI: 10.4236/ojmm.2016.64021
Abstract: Depending on its time of evolution, leptospirosis presents two phases: acute and chronic. The chronic leptospirosis is widely accepted in animals and, until recently, this was denied in humans. Since the 1990s, we have studied dozens of cases demonstrating the chronicity of leptospirosis, including an asymptomatic or undetermined form that may remain so throughout life time or may evolve into a definitive chronic disease and/or worsen, becoming severe cases. A fundamental part for its demonstration is the isolation and characterization of the etiologic agent. In this work, the first 14 isolations of Leptospira sp. are reported from human chronic leptospirosis and an African lion diagnosed with chronic leptospirosis, obtained between 2002 and 2006, in urine, blood, and skin microbiopsies. From the 14 isolations from patients with chronic leptospirosis, one of the cases is apparently congenital and two are from patients with chronic asymptomatic leptospirosis. The diagnosis was performed by means of videorecording in darkfield microscopy in blood and urine, specific culture media, and MAT serology. Argentic impregnation and detection of specific antigen by immunostaining in blood were performed in seven patients; in two patients, skin microbiopsies and biopsies were performed. All cases were positive by video-recording in darkfield, one of them MAT was 1:160, and the rest were positive at low titers (≤1:80). The two skin biopsies were positive by argentic impregnation and immunostaining. Eleven isolates were obtained from blood, two from skin, and one from urine. The isolates were characterized by monoclonal antibodies as Leptospira serovar Pomona. L. Pomona is considered of low virulence and high adaptability to the host, and is frequently associated to chronic leptospirosis in animals. In our study, it was the only isolated serovar, including the one isolated from the lion, which would seem the most common one in our country and responsible for a large portion of
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