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Search Results: 1 - 10 of 143146 matches for " Bal K Joshi "
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Association Mapping for Improvement of Quantitative Traits in Plant Breeding Populations
Umesh R Rosyara,Bal K Joshi
Nepal Journal of Biotechnology , 2012, DOI: 10.3126/njb.v2i1.5686
Abstract: DNA-based molecular markers have been extensively utilized for mapping of genes and quantitative trait loci (QTL) of interest based on linkage analysis in mapping populations. This is in contrast to human genetics that use of linkage disequilibrium (LD)-based mapping for fine mapping of QTLs using single nucleotide polymorphisms. LD based association mapping (AM) has promise to be used in plants. Possible use of such approach may be for fine mapping of genes / QTLs, identifying favorable alleles for marker aided selection and cross validation of results from linkage mapping for precise location of genes / QTLs of interest. In the present review, we discuss different mapping populations, approaches, prospects and limitations of using association mapping in plant breeding populations. This is expected to create awareness in plant breeders in use of AM in crop improvement activities.
Genetic Relationship among Nepalese Rice Landraces and Cultivars based on RAPD Markers
Bal K Joshi,Hari P Bimb,David Kansakar,Ekta Ghimire
Nepal Journal of Biotechnology , 2012, DOI: 10.3126/njb.v2i1.5635
Abstract: Genetic information of any genotype is necessary to manage and utilize them in conservation and breeding program. A total of 28 RAPD markers were used to relate the genetic structure among 50 Nepalese rice genotypes consisting of 29 landraces, 12 breeding lines and 9 released cultivars. Some of them are aromatic and blast resistance. Only four primers (P41, P60, P109 and P141) amplified the DNA of these genotypes with scorable bands. Primer 60 produced the highest number of bands (8). The highest number of present bands (6) was shown by primer 41 in 10 rice genotypes. Grouping of these genotypes based on the adaptation to agro-climatic zone was not observed, probably due to low percentage coverage of genome by four primers.? Most of the genotypes grouped in two clusters. Kali Marsi and IR-24 formed separate individual cluster. Mansara and Jarneli were the most similar landraces (0.96). Churenodhan and Pranpyuri were the most closely related with Masuli. Only one genotype NR-285-18 has fallen in the first quadrant by principal component (PC) analysis and the fourth quadrant was empty. The highest contribution in PC1 was from the second band of primer 41. This RAPD information can be used for selecting lines and for blast resistance breeding.
Hybridization Technique in Tartary Buckwheat (Fagopyrum tataricum Gaertn)
Bal K. Joshi,Hari P. Bimb,Kazutoshi Okuno
Nepal Agriculture Research Journal , 2009, DOI: 10.3126/narj.v9i0.11650
Abstract: N/A Nepal Agric. Res. J. Vol. 9, 2009, pp. 118-128 DOI: http://dx.doi.org/10.3126/narj.v9i0.11650 ?
Wheat gene pool and its conservation in Nepal
Bal K Joshi,Ashok Mudwari,Madan R Bhatta
Conservation Science , 2013, DOI: 10.3126/cs.v1i1.9584
Abstract: Aim This paper explores diversity of wheat gene pool present in the Nepalese bread wheat cultivars and landraces, and discusses their conservation initiatives. Location Nepal. Material and Methods This study is carried out using an extensive literature survey on distribution of landraces and wild relatives of wheat in Nepal. Key findings The results showed that there were 35 improved wheat cultivars, 540 landraces and 10 wild relatives of wheat in Nepal. Mexico, India and Nepal were the countries of origin for 35 cultivars. A total of 89 ancestors of wheat originated from 22 countries were used to develop 35 cultivars. The highest number of ancestors was from India. Ancestors of both aestivum and durum species having winter, spring and intermediate growth habit indicated that these species were of wide gene pool. The genetic erosion in wheat gene pool is the main conservation challenge of landraces due to introduction of improved varieties. Conservation implications Genetic diversity of wheat is indispensible for sustainable wheat production. Therefore, on-farm and ex-situ conservations of cultivars, landraces and wild varieties of wheat and their use in breeding programs are necessary for maintaining existing genetic diversity.? ? DOI: http://dx.doi.org/10.3126/cs.v1i1.9584 ? Conservation Science 2013 1(1), 39-46
RAPD Markers Linked to Late Blight Resistance in Tomato
Bal K Joshi,Dilip R Panthee,Frank J Louws,G Craig Yencho,Bryon Sosinski,Consuelo Arellano
Nepal Journal of Science and Technology , 2013, DOI: 10.3126/njst.v14i1.8871
Abstract: Identification of marker is the prerequisite for marker assisted selection (MAS) and is also very effective for gene pyramiding. Bulked Segregant Analysis (BSA) technique was used to identify RAPD markers linked to the late blight disease (caused by Phytopthora infestans (Mont. de Bary) resistance in tomato using F 2 population generated by crossing tomato inbred lines, NC 85L-1W(2007) which is resistant to late blight and NC 839-2(2007)-1 which is susceptible to it. A total of 250 F 2 plants, and 10 plants each of the parents and F 1 were used for BSA. Transgressive segregation was observed for late blight resistance. The segregation of susceptible and resistance perfectly fit the expected ratio of 3:1, that means resistance is governed by single recessive gene. Only 34 RAPD primers (17.26%) were found polymorphic between parents. Sixteen RAPD primers (47%) out of 34 gave polymorphic bands between resistant and susceptible bulks of the late blight. Four RAPD primers, namely MRTOMR-026, MRTOMR-031, MRTOMR-038 and MRTOMR-046 were identified as linked markers to loci related to late blight disease. Among those, two were linked to susceptible and two to resistance. Because of low reproducibility and dominant nature of RAPD, these markers need to be converted to SCAR markers. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 1-14 DOI: http://dx.doi.org/10.3126/njst.v14i1.8871
Heterosis for Yield and Yield Components in Rice
Bal Kumar Joshi
Nepal Agriculture Research Journal , 2000, DOI: 10.3126/narj.v4i0.4857
Abstract: It is important to know the degree and direction of hybrid vigor for its commercial exploitation. Heterobeltiosis and standard heterosis were studied in 14 crosses between rice ( Oryza sativa L.) cultivars (improved and landraces) and three wild aborted male sterile parents. These crosses showed marked variations in the expression of heterobeltiosis and standard heterosis for yield and yield components. Grain yield manifested highly significant heterobeltiosis and standard heterosis in five crosses. Heterobeltiosis ranging from –55 to 139% and standard heterosis from –11 to 369% were observed. Highest heterotic effect among the yield components was for panicle number plant-1 followed by spikelet number and panicle length. With appropriate choice of parental lines, it is possible to develop F1 rice hybrid possessing distinct yield superiority over the best-inbred lines. Key words: F1; Heterobeltiosis; Hybrid vigor; Oryza sativa ; Standard heterosis DOI: http://dx.doi.org/10.3126/narj.v4i0.4857 Nepal Agriculture Research Journal Vol. 4&5, 2001/2002 Page: 6-12 Uploaded date: 8 June, 2011
Measles deaths in Nepal: estimating the national case-fatality ratio
Joshi,Anand B; Luman,Elizabeth T; Nandy,Robin; Subedi,Bal K; Liyanage,Jayantha BL; Wierzba,Thomas F;
Bulletin of the World Health Organization , 2009, DOI: 10.1590/S0042-96862009000600014
Abstract: objective: to estimate the case-fatality ratio (cfr) for measles in nepal, determine the role of risk factors, such as political instability, for measles mortality, and compare the use of a nationally representative sample of outbreaks versus routine surveillance or a localized study to establish the national cfr (ncfr). methods: this was a retrospective study of measles cases and deaths in nepal. through two-stage random sampling, we selected 37 districts with selection probability proportional to the number of districts in each region, and then randomly selected within each district one outbreak among all those that had occurred between 1 march and 1 september 2004. cases were identified by interviewing a member of each and every household and tracing contacts. bivariate analyses were performed to assess the risk factors for a high cfr and determine the time from rash onset until death. each factor's contribution to the cfr was determined through multivariate logistic regression. from the number of measles cases and deaths found in the study we calculated the total number of measles cases and deaths for all of nepal during the study period and in 2004. findings: we identified 4657 measles cases and 64 deaths in the study period and area. this yielded a total of about 82 000 cases and 900 deaths for all outbreaks in 2004 and a national cfr of 1.1% (95% confidence interval, ci: 0.5-2.3). cfr ranged from 0.1% in the eastern region to 3.4% in the mid-western region and was highest in politically insecure areas, in the ganges plains and among cases < 5 years of age. vitamin a treatment and measles immunization were protective. most deaths occurred during the first week of illness. conclusion: to our knowledge, this is the first cfr study based on a nationally representative sample of measles outbreaks. routine surveillance and studies of a single outbreak may not yield an accurate ncfr. increased fatalities associated with political insecurity are a challenge for healt
Synthesis and Characterization of Some Trivalent and Tetravalent Derivatives of 2- Hydroxy-1-naphthoic Acid
Deepti Joshi,T. K. Joshi
Journal of Chemistry , 2004, DOI: 10.1155/2004/405086
Idiopathic pulmonary haemosiderosis with mineralizing pulmonary elastosis: A case report
Amanjit Bal, Ashish Bhalla, Kusum Joshi
Journal of Medical Case Reports , 2008, DOI: 10.1186/1752-1947-2-65
Abstract: The case presented here is of an adult man with idiopathic pulmonary haemosiderosis with mineralizing pulmonary elastosis.Pathologists are generally not familiar with this histologic reaction pattern associated with iron encrustation of pulmonary elastic tissue.Diffuse pulmonary haemosiderosis is characterized by repeated episodes of intra-alveolar haemorrhage leading to abnormal accumulation of iron as haemosiderin in alveolar macrophages with subsequent pulmonary fibrosis and severe anaemia [1,2]. Pulmonary haemosiderosis (PH) occurs either primarily as a disease of the lungs or secondary to systemic diseases. Idiopathic PH, first described by Ceelen in 1931, is characterized by a clinical triad of haemoptysis, anaemia and pulmonary infiltrates. Eighty percent of cases of PH occur in children [2]. We report a case of an adult man with idiopathic PH with mineralizing pulmonary elastosis.A 32-year-old, non-smoking, male farmer presented with a history of fever, intermittent episodes of mild haemoptysis and cough with expectoration for the previous six months. There was associated loss of appetite and loss of weight. He started experiencing respiratory distress 8 days prior to hospital admission. Two years earlier he was diagnosed with pulmonary tuberculosis based on X-ray findings whereupon he was commenced on anti-tubercular treatment, however the level compliance is not known. On physical examination his respiratory rate and jugular venous pressure were elevated, and he had clubbing. On chest auscultation there were bilateral coarse crepitations. Pulmonary function tests were consistent with restrictive ventilatory defect. Laboratory investigations revealed iron deficiency anaemia and there were negative results for antinuclear antibodies (ANA) and antineutrophilic cytoplasmic antibodies (ANCA). On ultrasound examination of the abdomen there was evidence of hepatosplenomegaly. The clinical impression was of disseminated tuberculosis. The patient was placed on ambu
Influence of TCSC on social welfare and spot price - A comparative study of PSO with classical method
S Joshi, K Pandya
International Journal of Engineering, Science and Technology , 2010,
Abstract: In this paper, Particle Swarm Optimization (PSO) based algorithm has been suggested to find optimal location and setting of Thyristor Controlled Series Compensator (TCSC) to maximize Social Benefit (SB), considering its installation cost in competitive electricity market. PSO has simultaneously optimized generators’ active powers output, generators’ bus voltages, TCSC reactance and its location. In addition, the influence of optimally located TCSC on the magnitude of real power spot price, reactive power spot price, wheeling charges and bilateral transaction matrix has been investigated. The effectiveness of the proposed approach has been tested on IEEE 6 bus system and results obtained are compared with those obtained from various classical methods.
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