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Search Results: 1 - 10 of 18 matches for " Bajoghli Baubak "
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Side chain modified peptide nucleic acids (PNA) for knock-down of six3 in medaka embryos
Dorn Sebastian,Aghaallaei Narges,Jung Gerlinde,Bajoghli Baubak
BMC Biotechnology , 2012, DOI: 10.1186/1472-6750-12-50
Abstract: Background Synthetic antisense molecules have an enormous potential for therapeutic applications in humans. The major aim of such strategies is to specifically interfere with gene function, thus modulating cellular pathways according to the therapeutic demands. Among the molecules which can block mRNA function in a sequence specific manner are peptide nucleic acids (PNA). They are highly stable and efficiently and selectively interact with RNA. However, some properties of non-modified aminoethyl glycine PNAs (aegPNA) hamper their in vivo applications. Results We generated new backbone modifications of PNAs, which exhibit more hydrophilic properties. When we examined the activity and specificity of these novel phosphonic ester PNAs (pePNA) molecules in medaka (Oryzias latipes) embryos, high solubility and selective binding to mRNA was observed. In particular, mixing of the novel components with aegPNA components resulted in mixed PNAs with superior properties. Injection of mixed PNAs directed against the medaka six3 gene, which is important for eye and brain development, resulted in specific six3 phenotypes. Conclusions PNAs are well established as powerful antisense molecules. Modification of the backbone with phosphonic ester side chains further improves their properties and allows the efficient knock down of a single gene in fish embryos.
Rapid identification of PAX2/5/8 direct downstream targets in the otic vesicle by combinatorial use of bioinformatics tools
Mirana Ramialison, Baubak Bajoghli, Narges Aghaallaei, Laurence Ettwiller, Sylvain Gaudan, Beate Wittbrodt, Thomas Czerny, Joachim Wittbrodt
Genome Biology , 2008, DOI: 10.1186/gb-2008-9-10-r145
Abstract: Here we present an original bioinformatics pipeline composed of comparative genomics, database querying and text mining tools, which is designed to rapidly and specifically discover PAX2/5/8 direct downstream targets involved in inner ear development. We provide evidence supported by experimental validation in medaka fish that brain 2 (POU domain, class 3, transcription factor 2), claudin-7, secretory pathway component sec31-like and meteorin-like precursor are novel direct downstream targets of PAX2/5/8.This study illustrates the power of extensive mining of public data repositories using bioinformatics methods to provide answers for a specific biological question. It furthermore demonstrates how the usage of such a combinatorial approach is advantageous for the biologist in terms of experimentation time and costs.The pax genes encode a family of transcription factors that have been conserved through evolution and play different roles in early development. This family is defined by the presence of a highly conserved motif of 128 amino acids, the paired-domain, which does not have any obvious sequence homology with other known protein domains. Nine members of the pax gene family have been isolated in vertebrates, which are grouped into four distinct subfamilies, based on sequence similarity and structural domains [1-3]. The subfamily consisting of PAX2, PAX5 and PAX8 (PAX2/5/8) encodes transcription regulators that bind DNA via the amino-terminal paired-domain, whereas the carboxy-terminal region is required for trans-activation or repression of target genes. Detailed DNA binding studies led to the definition of a consensus recognition sequence that is bound by all members of this subfamily [4,5]. The pax2/5/8 genes are expressed in a spatially and temporally overlapping manner in the brain, eye, kidney and inner ear in several model organisms [6-9]. Particularly, the members of this subfamily are the earliest known genes that are involved in inner ear development.
CANCER PREDISPOSITION SYNDROMES IN CHILDREN
M. Bajoghli
Iranian Journal of Radiology , 2012,
Abstract: The term cancer predisposition syndrome (CPS)includes several familial cancers in which a clear mode of inheritance may be established, although a specific gene defect has not been described in all cases.Advance in genetics and the development of new imaging have led to better understanding and early detection of these syndromes and offer the diagnosis of any associated tumors. As a result, imaging has become an essential component to management of CPSs and the care ofchildren with neurofibromatosis type I, Beckwith- Wiedemann syndrome, Von Hippel-Lindau (VHL), multiple endocrine neoplasia (MEN), familial adenomatous polyp and other syndromes. A radiologist should be familiar with these syndromes, their common associated tumors and thenew imaging techniques that are available to optimize the assessment of affected children.Of course recent advance in genetics has led to better understanding and early recongnition of these diseases and proper genetic counseling helps these patients.
The Neonate with Abdominal Mass
M. Bajoghli
Iranian Journal of Radiology , 2008,
Abstract: Neonatal abdominal masses have broad spectrum of pathology, ranging from small lesions found incidentally to large masses occupying the entire of peritoneal cavity. These tumors are benign to malignant, and from unilocular cysts to complex solid lesions. Many of these lesions identified and will treat before delivery. Others are discovered during routine examination. These lesions may be life threatening, or cause persistent morbidity. Some of these have no sequel."nDiagnosis began with history. Characteristic of the mass which must be note include location, size, shape, texture, mobility and tenderness. Other findings should be in mind to find out nature of mass, for example hypoplasia of chest wall with oligohydramnios due to GU tract obstruction (potter sequwnce), a bulging hymen due to hydrometrocolpus, skin metastasis due to neuroblastoma. Radiography is the next step that shows organomegaly and calcification. Next step is US which is an excellent screening tool. CT and MRI are occasionally indicated."nThe purpose of this presentation is to review the diagnosis of abdominal masses in neonates.
Classic Signs in Imaging in Pediatric Disease
M. Bajoghli
Iranian Journal of Radiology , 2008,
Abstract: The language of radiology is rich with description of imaging findings, often metaphorical, which have found common usages in the practice of pediatric radiology. These "classic signs" give confidence in our diagnosis. Some of the signs have become so familiar to us and often bring an impression of the image to mind, and make specific diagnostic and pathologic finding . Description of observation made from images is the radiologist's stock in trade. This article uses classic signs accumulated from the literature to review variety pathologic conditions in pediatric radiology.
All the Information the Neonate and Infant Skull
M. Bajoghli
Iranian Journal of Radiology , 2008,
Abstract: The art of skull interpretation slowly being lost and trainees in radiology see few plane film of skull. However, skull radiography still provides significant information that is helpful in finding pathologic conditions. Abnormality of skull may be reflected as variation in density, size and shape of the skull, as well as skull defect. Skull dysplasia may manifest as decreased calvarial density (hypophosphatasia, osteogenesis imferfecta), or increased calvarial density (osteopetrosis), or facial increase in density (frontometaphyseal dysplasia). Diffusely decreased or increased clavarial density is more associated with process that affects entire skull. Decrease density of cranial vault at birth seen more in torch and fetal alcoholic. Macrocephaly may be due to skeletal sysplasia, or hydrocephalus. The aim of this review is to evaluate plane film of skull in neonate and infants.
Primary Cutaneous CD30-Positive Large T-Cell Lymphoma in an 80-Year-Old Man: A Case Report
Rehan Hussain,Amir Bajoghli
ISRN Dermatology , 2011, DOI: 10.5402/2011/634042
Abstract:
Second Malignancy in Pediatric Patients: Imaging Findings and Differential Diagnosis
M. Bajoghli,N. Tayari
Iranian Journal of Radiology , 2010,
Abstract: Therapeutic advances in the treatment of pediatric neoplasms have improved the prognosis but have also increased the risk of developing rare second malignancies."nPrimary neoplasms which are often associated with second malignancies include lymphoma, retinoblas-toma, medulloblastoma and leukemia. The most common second malignancies are central nervous system (CNS) tumors, sarcomas, thyroid and parathyroid gland carcinoma and leukemia, particularly acute myeloblastic leukemia. Genetic predisposition, chemotherapy, and especially radiation therapy are included as pathogenic factors in second malignancies. We know all survivors of childhood cancer should have lifelong follow-up, preferably with US, magnetic resonance imaging and other procedures with no ionizing radiation. A new progressive lesion may represent recurrence of the primitive neoplastic process, late radiation injury, or more infrequently, a second malignancy. Differential diagnosis may be very difficult and the outcome is often fatal. "nTreatment protocols should be modified to reduce the risk of second malignancies without compromising the effectiveness of initial therapy. "nClinicians should individualize treatment for patients who are genetically predisposed. In addition, radiologists should be familiar with the long-term consequences of antineoplastic therapy."nIn a report of new England journal of medicine in 2007, 357:227-2284 by Dr. Brenner and Hall, 2% of all carcinomas in U.S.A are due to more use of CT exam and children are three to four times more sensitive to ionization radiation. "nSo all the radiologists and clinicians should be aware of the complications and should recommend follow up exams in children who have had previous treatments for such carcinomas.
Effects of cigarette smoking on priapism induced by quetiapine: a case report
Seyed Hosseini, Hafez Bajoghli, Padideh Ghaeli
DARU Journal of Pharmaceutical Sciences , 2012, DOI: 10.1186/2008-2231-20-55
Abstract: The etiology of priapism may be idiopathic, drug-induced, or related to some medical problems like sickle cell disease or leukemia [1]. Priapism has been notenoted as an adverse effect of both first- and second-generation anti psychotic medications [2]. Peripheral alpha-1 adrenergic blockade has been considered as the main cause of priapism [3]. Trazodone with its alpha-1 antagonistic properties, has been reported to induce priapism [4]. Antipsychotics are accounted for 15-26% of priapism cases that are induced by medications [5]. Previous case reports of priapism associated with quetiapine were those with a total daily dose of 600mg or greater [4,6-9]. One case involved a single overdose of 675mg [8]. Presently, we report a case of priapism associated with 25mg use of quetiapine [10].A 30 year old single unemployed man was presented to our out-patient psychiatry clinic because of a recurrence of the psychotic episode. The patient did not have any comorbidity with medical diseases or substance abuse disorders in the past. Recurrence of the psychotic episode was due to the self-discontinuation of quetiapine secondary to recurrent priapism. He was diagnosed with paranoid schizophrenia since 10 years ago. His main symptoms included delusions of control, persecutory delusion, and auditory hallucination. His symptoms were relatively controlled. He had several recurrences and all of them were associated with drug discontinuation due to the patient's lack of compliance. He did not have any major stressors before recurrences. He was under treatment of first- and second-generation antipsychotics. He developed drug-induced Parkinsonism several times which was resolved with dose-adjustment and anti cholinergic therapy. The patient experienced priapism secondary to use of some antipsychotic medications like risperidone, olanzapine, clozapine and finally quetiapine. The diagnosis of priapism was confirmed by a urologic consult. All medical causes of priapism were ruled-out by a
To Determine and Compare the Average Amounts of Resistance of Four Features of Preparation on First Mandibular Molar Full Metal Crowns
farshad - bajoghli,p maleki,r mehrvarzan
Journal of Isfahan Dental School , 2006,
Abstract: Introduction: Full coverage of teeth chosen correctly, could be the best treatment, but because of different steps involved in crown fabrication, dentists must try to make crowns with noticeable duration. The purpose of this study was to evaluate the effect of auxillary preparation elements and occlusal surface modifications on the resistance form of a complete lower molar crown.Methods and materials: This study was of experimental-laboratory type. Ivorine tooth was prepared with 20 degree TOC (Total Occlusal Convergence), 3 mm occlusocervical dimension and shoulder finishing line (First group=control group). Ten standard metal dies were replicated from this model. Standard metal crowns were made for all samples. After the cementation of metal crowns to metal dies with resin cement, the resistance of each samples against lateral forces were determined by instron machine. Next groups consisted of interproximal boxes+ buccolingual grooves (2nd group), occlusal isthmus (3rd group) and reduced TOC at cervical area (4th group) prepared on control group. The other procedures were done the same as for the control group. Data from 4 groups were evaluated by ANOVA and Post Hoc tests.Results: Statistical analysis of the results showed the most significant differences were in the 4th group and then the second group (239.1 and 178.5 kgf respectively). While no statistical differences were found between groups "1&3" (93.8 and 94.7 kgf respectively).Conclusion: The best axial preparation to increase the resistance form was reduced TOC. Preparing boxes+grooves were of second impatance. However lack of axial preparation (group "A") or auxiliary features without favorable effects such as occlusal isthmus (group "C") on teeth with insufficient resistance form does not have any positive effect.Key words: Resistance, Axial preparation, Total occlusal convergence.
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