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Evaluations of antidepressant activity of Anacyclus pyrethrum root extract
Badhe S,Badhe R,Ghaisas M,Chopade V
International Journal of Green Pharmacy , 2010,
Abstract: The present study was designed to screen antidepressant activity of Anacyclus pyrethrum (AP) root extract. An experiment was designed by different method such as Locomotor activity, Haloperidol-induced catalepsy, Forced swim test (FST), Tail suspension test (TST), Clonidine-induced hypothermia and Reserpine-induced hypothermia on Swiss male albino mice. Standard root extract of Anacyclus pyrethrum (AP root extract) showed an increase in ambulatory behaviour indicating a stimulant effect of the photoactometer. AP root extract produces a significant antidepressant effect in both FST and TST as they reduced the immobility. AP root extract was found to be effective in reversing hypothermia produced by clonidine and reserpine. In our study, we found that AP root extract inhibited haloperidol-induced catalepsy. These study suggest that AP root extract might produce antidepressant effect by interaction with adrenergic and dopamine receptor thereby increasing the level of noradrenaline and dopamine in brains of mice.
The action of red scorpion (Mesobuthus tamulus coconsis, pocock) venom and its isolated protein fractions on blood sodium levels
Badhe, R. V.;Thomas, A. B.;Deshpande, A. D.;Salvi, N.;Waghmare, A.;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2007, DOI: 10.1590/S1678-91992007000100006
Abstract: red scorpion (mesobuthus tamulus or buthus tamulus) venom samples were collected at different regions of india: western (chiplun and ahmednagar from maharashtra state) and southern (ratnagiri and chennai from tamil nadu state). the action of whole venoms on the blood sodium levels of mice was assessed using flame photometry. seven peptides were common to all venom samples. they were separated using the native polyacrylamide gel electrophoresis (page) technique and their activities were also studied using flame photometry. there was a decrease in the concentration of sodium ions in the serum, which suggested the blockage of such ions by scorpion venom toxins. among the 10 protein bands isolated, the band at 79.6 kda presented maximum activity in decreasing serum sodium ions concentration. whole venom from chiplun region also showed maximum activity. the western blotting technique demonstrated that the anti-scorpion venom sera produced by haffkine biopharmaceuticals corporation ltd., india, neutralized all four venom samples.
Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India
Badhe, R. V.;Thomas, A. B.;Harer, S. L.;Deshpande, A. D.;Salvi, N.;Waghmare, A.;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2006, DOI: 10.1590/S1678-91992006000400008
Abstract: red scorpions mesobuthus tamulus (coconsis, pocock) were obtained from different regions of west and south india (ratnagiri, chiplun and ahmednagar from maharashtra and chennai from tamil nadu, respectively). their venoms composition was analyzed using gel electrophoresis (sds-page). all venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kda molecular weights. bands of 115 kda and 51.5 kda were characteristic of venoms obtained from red scorpions of chiplun region, and the 26kda band was absent in scorpion venom from tamil nadu. the separated protein band patterns suggest that the venoms from ratnagiri, ahmednagar and tamil nadu had high similarities in their biochemical composition but differed from that of chiplun region. these data were also supported by the jaccard (j) index. the j value was 0.33 for venom obtained from ratnagiri-ahmednagar, 0.31 for venom from ratnagiri-tamil nadu, and 0.3 for venom from ratnagiri-chiplun region. this suggests the existence of genetic variation among the different strains of red scorpion in western and southern india. the antiserum produced by haffkine biopharmaceuticals corporation ltd. completely neutralized proteins of venoms from all the regions studied.
Effect of Aqueous Extract and Essential Oils of Ginger and Garlic as Decontaminat in Chicken Meat
S. Sudarshan,Nadeem Fairoze,S. Wilfred Ruban,Shekar R. Badhe,B.V. Raghunath
Research Journal of Poultry Sciences , 2012, DOI: 10.3923/rjpscience.2010.58.61
Abstract: A study was conducted to evaluate efficiency of aqueous extract and essential oils of ginger and garlic as decontaminating agent in chicken meat was carried out. Two concentrations of Aqueous Extract-AE (100 and 75%) and three concentrations of Essential oil-EO (1:150, 1:250 and 1:500) of ginger and garlic were for the study. Results revealed that Aqueous extract of ginger at 100 and 75% concentration did not have any effect in terms of viable log reduction with respect to the bacterial pathogens whereas essential oil of ginger at 1:150 and 1:250 concentrations brought about a significant decrease in bacterial count compared to 1:500. Based on the results it was observed that 1:150 concentration of EO of ginger was best among the different treatments used. A highly significant (p<0.01) reduction in bacterial counts by one log with respect to TVC, Staphylococcal counts, E. coli and Salmonella was observed following dipping of chicken meat in AE of garlic at 100% concentration. The efficiency of decontamination ability of AE of garlic was found better compared to EO. The order of various concentrations in causing bacterial reduction was 100% followed by 75% AE, 1:150, 1:250 and 1:500 concentration of EO. Aqueous extract of garlic was found to be better in decreasing the microbial load compared to other treatments.
An Evaluation of Antibacterial Activity of Abelmoschus esculentus on Clinically Isolated Infectious Disease Causing Bacterial Pathogen from Hospital
Yogesh Chaudhari,Dr. E. P. Kumar,Manisha Badhe,Hardik R. Mody
International Journal of Pharmaceutical and Phytopharmacological Research , 2012,
Abstract: Six different organic solvents such as n-butanol, petroleum ether, methanol, ethyl acetate and chloroform were used to extract the bioactive compounds from the fruits of Abelmoschus esculentus to screen the antibacterial activity against infectious disease causing bacterial pathogens such as Bacillus subtilis, Streptococcus pyogens, Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Proteus mirabillis and Pseudomonas aeruginosa by paper disc method. The butanolic extract of Abelmoschus esculentus was more active against almost 90% of the organism tested. It was followed by Ethyl acetate, Methanol, Petroleum ether, Chloroform in inhibiting the growth of organism tested
An intravenous organophosphate poisoning with intermediate syndrome: An unusual way of intoxication
Badhe Ashok,Sudhakar S
Indian Journal of Critical Care Medicine , 2006,
Abstract: Organophosphate (OP) poisoning is very common form of poisoning in Indian population because of its availability and easy access. Intoxication occurs following the absorption of OP agents through gastrointestinal tract, skin and respiratory tract and rarely by intramuscular or intravenous route. The clinical features depend on the amount of the poison consumed, it′s concentration, the route of administration and the time of instituting therapy. We are reporting a case of an intravenous monochrotophos poisoning, an unusual way of intoxication, which was managed in our ICU.
Inhaled nitroglycerin in the treatment of pulmonary hypertension
Parida Satyen,Badhe Ashok
Annals of Cardiac Anaesthesia , 2011,
Abstract:
Nephroblastomatosis--pathologic and imaging characteristics.
Merchant S,Badhe P
Journal of Postgraduate Medicine , 1995,
Abstract:
Large optic nerve glioma.
Badhe P,Desai A
Journal of Postgraduate Medicine , 1996,
Abstract: Twelve cases of optic nerve glioma seen over a 28 year period are analysed herein in this autopsy study.
Xanthogranulomatous pyelonephritis--(a clinicopathological study of 15 cases)
Mittal B,Badhe B
Journal of Postgraduate Medicine , 1989,
Abstract: The clinico-pathological features of 15 patients with xanthogranulomatous pyelonephritis (XGP) are described and the probable histogenesis is discussed. Based on our data and the review of literature, we believe that XGP should be regarded as a destructive and at times tumefactive inflammatory process that may complicate chronic pyelonephritis. The initiation of this process remains obscure, but the features commonly associated with XGP are pelvi-calyceal obstruction, ulceration of the pelvic urothelium with collection of necrotic material and bacterial infection.
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