Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99


Any time

2019 ( 10 )

2018 ( 37 )

2017 ( 26 )

2016 ( 26 )

Custom range...

Search Results: 1 - 10 of 4006 matches for " Aya Takahashi "
All listed articles are free for downloading (OA Articles)
Page 1 /4006
Display every page Item
Effect of exonic splicing regulation on synonymous codon usage in alternatively spliced exons of Dscam
Aya Takahashi
BMC Evolutionary Biology , 2009, DOI: 10.1186/1471-2148-9-214
Abstract: Codon Bias Indices (CBI) in the 3' boundary regions were reduced compared to the rest of the exonic regions among 48 and 33 ASEs of exon 6 and 9 clusters, respectively. These regional differences in CBI were affected by splicing frequency and distance from adjacent exons. Synonymous divergence levels between the 3' boundary region and the remaining exonic region of exon 6 ASEs were similar. Additionally, another sensitive comparison of paralogous exonic regions in recently retrotransposed processed genes and their parental genes revealed that, in the former, the differences in CBI between what were formerly the central regions and the boundary regions gradually became smaller over time.Analyses of the multiple ASEs of Dscam allowed direct tests of the effect of splice-related factors on synonymous codon usage and provided clear evidence that synonymous codon usage bias is restricted by exonic splicing signals near the intron-exon boundary. A similar synonymous divergence level between the different exonic regions suggests that the intensity of splice-related selection is generally weak and comparable to that of translational selection. Finally, the leveling off of differences in codon bias over time in retrotransposed genes meets the direct prediction of the tradeoff model that invokes conflict between translational superiority and splicing regulation, and strengthens the conclusions obtained from Dscam.Genomic data from many different organisms indicate that synonymous codon usage is typically biased. This bias is an intriguing property that reflects a composite of different evolutionary forces such as mutational bias, genetic drift, and natural selection [1-3]. In many species, including Drosophila, one of the dominant forces affecting the bias is thought to be natural selection for translational efficiency or accuracy, or both (reviewed in [4]). The most abundant codons are preferred because of the abundance of the cognate tRNAs [5-9], and thus the use of the cod
Telemonitoring of Patients with Implantable Cardiac Devices to Manage Heart Failure: An Evaluation of Tablet-PC-Based Nursing Intervention Program  [PDF]
Aya Umeda, Tomoko Inoue, Takuri Takahashi, Hidetoshi Wakamatsu
Open Journal of Nursing (OJN) , 2014, DOI: 10.4236/ojn.2014.44028

Background: With the rapid aging of society, the number of patients with heart failure has also increased. Implantable devices for heart failure have become standardized. Remote monitoring using cardiac devices has grown in popularity for medical efficiency and the early detection of abnormalities. Our first aim was to develop a tablet-PC-based nursing intervention program for patients requiring remote monitoring of implantable cardiac devices. The second purpose was to evaluate the efficacy of the program by using mixed-methods research. Methods: The study consisted of two phases. In phase 1, we designed a tablet-PC-based nursing intervention program, on the basis of a literature review and qualitative data collected via semi-structured interviews. In phase 2, we conducted a randomized controlled trial that served as a preliminary investigation of the program. The outcome measures were readmission, unexpected visits to the clinic for heart problems, quality of life, self-care behavior, and self-efficacy. After the study, we interviewed each participant about his or her experiences with the program. Interviews were audio recorded, coded, and thematically analyzed. Results: The 33 patients with heart failure were randomized into two groups as follows: 17 patients in the telenursing group and 16 in the control group. During 6 months of follow-up, the readmission for heart failure occurred in 11% of the intervention group and 18% of the control group. There were no statistically significant differences between the groups at any outcome measures. Three themes were extracted via qualitative analysis: “getting a sense of safety,” “triggering a health behavior change,” and “feeling like a burden.” Conclusion: No improvements in rates of rehospitalization or unexpected clinic visits were seen in the quantitative study. However, signs of behavior modification were seen in the qualitative study. This program has the possibility of improving patient outcomes.

Therapeutic Comparison between Sun Irradiation vs. Narrowband UVB Phototherapy along with Concomitant Topical Tacalcitol for Vitiligo Vulgaris  [PDF]
Atsushi Tanemura, Aya Takahashi, Yuriko Ueki, Hiroyuki Murota, Yuji Yamaguchi, Ichiro Katayama
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2012, DOI: 10.4236/jcdsa.2012.22020
Abstract: In addition to therapeutic options such as steroid ointment, immunosuppressive drug and ultraviolet phototherapy, recent reports have demonstrated the clinical efficacy of topical vitamin D3 for vitiligo vulgaris. However, there seems to be little clinical effect of vitamin D3 without UV exposure. In the present study, we evaluated the clinical difference of sun irradiation vs. narrowband UVB in combination with tacalcitol assessing the change of lesion size and color tone by a spectrophotometer. Thirty-three vitiligo patients were composed of 19 treated with sun illumination and 14 treated with narrowband UVB in combination with topical use of tacalcitol. The mean % size reduction was higher in the group of sunbathing than narrowband UVB (29% vs. 23%). Delta L score, which represented the difference of whiteness between lesional and perilesional skin, was significantly improved after treatment in the group of sun irradiation instead of narrowband UVB (p = 0.0023). Therefore, we consider that sun illumination along with tacalcitol may be able to induce natural repigmentation and be an alternative therapeutic option for vitiligo vulgaris.
Erratum: Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons
Tatsuya Yoshikawa,Bumpei Samata,Aya Ogura,Jun Takahashi
Frontiers in Cellular Neuroscience , 2013, DOI: 10.3389/fncel.2013.00116
Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell–derived dopaminergic neurons
Tatsuya Yoshikawa,Bumpei Samata,Aya Ogura,Jun Takahashi
Frontiers in Cellular Neuroscience , 2013, DOI: 10.3389/fncel.2013.00011
Abstract: Cell replacement therapy using embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is a promising strategy for the treatment of neurologic diseases such as Parkinson's disease (PD). However, a limiting factor for effective cell transplantation is the low survival rate of grafted cells, especially neurons. In this study, we modified the host environment and investigated whether the simultaneous administration of soluble factors can improve the survival and differentiation of murine iPSC-derived dopaminergic (DA) neurons in host brains. With the goal of applying this technology in clinical settings in the near future, we selected drugs that were already approved for clinical use. The drugs included two commonly used anti-convulsants, valproic acid (VPA) and zonisamide (ZNS), and estradiol (E2), also known as biologically active estrogen. Following neural induction of murine iPSCs, we collected neural progenitor cells (NPCs) by sorting PSA-NCAM+ cells, then treated the PSA-NCAM+ cells with drugs for 4 days. An immunofluorescence study revealed that 0.01 mM and 0.1 mM of VPA and 10 nM of E2 increased the percentage of tyrosine hydroxylase+ (TH: a DA neuron marker) cells in vitro. Furthermore, 0.1 mM of VPA increased the percentage of TH+ cells that simultaneously express the midbrain markers FOXA2 and NURR1. Next, in order to determine the effects of the drugs in vivo, the iPSC-derived NPCs were transplanted into the striata of intact SD rats. The animals received intraperitoneal injections of one of the drugs for 4 weeks, then were subjected to an immunofluorescence study. VPA administration (150 mg/kg/daily) increased the number of NeuN+ post-mitotic neurons and TH+ DA neurons in the grafts. Furthermore, VPA (150 mg/kg/daily) and ZNS (30 mg/kg/daily) increased the number of TH+FOXA2+ midbrain DA neurons. These results suggest that the systemic administration of VPA and ZNS may improve the efficiency of cell replacement therapy using iPSCs to treat PD.
X-ray investigation of the diffuse emission around plausible gamma-ray emitting pulsar wind nebulae in Kookaburra region
Tetsuichi Kishishita,Aya Bamba,Yasunobu Uchiyama,Yasuyuki Tanaka,Tadayuki Takahashi
Physics , 2012, DOI: 10.1088/0004-637X/750/2/162
Abstract: We report on the results from {\it Suzaku} X-ray observations of the radio complex region called Kookaburra, which includes two adjacent TeV $\gamma$-ray sources HESS J1418-609 and HESS J1420-607. The {\it Suzaku} observation revealed X-ray diffuse emission around a middle-aged pulsar PSR J1420-6048 and a plausible PWN Rabbit with elongated sizes of $\sigma_{\rm X}=1^{\prime}.66$ and $\sigma_{\rm X}=1^{\prime}.49$, respectively. The peaks of the diffuse X-ray emission are located within the $\gamma$-ray excess maps obtained by H.E.S.S. and the offsets from the $\gamma$-ray peaks are $2^{\prime}.8$ for PSR J1420-6048 and $4^{\prime}.5$ for Rabbit. The X-ray spectra of the two sources were well reproduced by absorbed power-law models with $\Gamma=1.7-2.3$. The spectral shapes tend to become softer according to the distance from the X-ray peaks. Assuming the one zone electron emission model as the first order approximation, the ambient magnetic field strengths of HESS J1420-607 and HESS J1418-609 can be estimated as 3 $\mu$G, and $2.5 \mu$G, respectively. The X-ray spectral and spatial properties strongly support that both TeV sources are pulsar wind nebulae, in which electrons and positrons accelerated at termination shocks of the pulsar winds are losing their energies via the synchrotron radiation and inverse Compton scattering as they are transported outward.
Cuticular Hydrocarbon Content that Affects Male Mate Preference of Drosophila melanogaster from West Africa
Aya Takahashi,Nao Fujiwara-Tsujii,Ryohei Yamaoka,Masanobu Itoh,Mamiko Ozaki,Toshiyuki Takano-Shimizu
International Journal of Evolutionary Biology , 2012, DOI: 10.1155/2012/278903
Abstract: Intraspecific variation in mating signals and preferences can be a potential source of incipient speciation. Variable crossability between Drosophila melanogaster and D. simulans among different strains suggested the abundance of such variations. A particular focus on one combination of D. melanogaster strains, TW1(G23) and Mel6(G59), that showed different crossabilities to D. simulans, revealed that the mating between females from the former and males from the latter occurs at low frequency. The cuticular hydrocarbon transfer experiment indicated that cuticular hydrocarbons of TW1 females have an inhibitory effect on courtship by Mel6 males. A candidate component, a C25 diene, was inferred from the gas chromatography analyses. The intensity of male refusal of TW1 females was variable among different strains of D. melanogaster, which suggested the presence of variation in sensitivity to different chemicals on the cuticle. Such variation could be a potential factor for the establishment of premating isolation under some conditions. 1. Introduction Drosophila exhibits complex mating behavior with frequent wing vibration and copulation attempts by males. The successful mating is achieved by communications between males and females using chemical, acoustic, and visual signals (reviewed in [1]). Subtle differences in these signals may accumulate during or after the formation of reproductive isolation. Once reproduction isolation is established to a certain extent, the correct mate recognition is essential to avoid costly hybridization and wasting time on unsuccessful courtship. Indeed, a certain degree of premating isolation or mating incompatibility is commonly observed between closely related species of Drosophila [2, 3]. In some cosmopolitan species of Drosophila, for example, D. ananassae [4] and D. elegans [5, 6], widely observed mating incompatibilities between populations from different locations exist. The degree of incompatibility is variable among sampled strains in these species. Another cosmopolitan species, D. melanogaster, also harbors incompatible combinations of populations [7–11]. The degree of incompatibility between populations is also variable, and many intermediate strains are typically observed. These within species incompatibilities suggest that there are many intraspecific variations in mating signals and preferences. Those variations could either fix in isolated populations or become targets of sexual selection under some conditions and consequently result in divergent mating-associated characters among different populations. It is
Investigation of Spatial Distribution of Radiocesium in a Paddy Field as a Potential Sink
Kazuya Tanaka, Hokuto Iwatani, Yoshio Takahashi, Aya Sakaguchi, Kazuya Yoshimura, Yuichi Onda
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0080794
Abstract: Surface soils, under various land uses, were contaminated by radionuclides that were released by the Fukushima Daiichi Nuclear Power Plant accident. Because paddy fields are one of the main land uses in Japan, we investigated the spatial distribution of radiocesium and the influence of irrigation water in a paddy field during cultivation. Soil core samples collected at a paddy field in Fukushima showed that plowing had disturbed the original depth distribution of radiocesium. The horizontal distribution of radiocesium did not show any evidence for significant influence of radiocesium from irrigation water, and its accumulation within the paddy field, since the original amount of radiocesium was much larger than was added into the paddy field by irrigation water. However, it is possible that rainfall significantly increases the loading of radiocesium.
The effectiveness of a multidisciplinary QI activity for accidental fall prevention: Staff compliance is critical
Sachiko Ohde, Mineko Terai, Aya Oizumi, Osamu Takahashi, Gautamn A Deshpande, Miwako Takekata, Ryoichi Ishikawa, Tsuguya Fukui
BMC Health Services Research , 2012, DOI: 10.1186/1472-6963-12-197
Abstract: This observational study was conducted from July 2004 through December 2010 at St. Luke’s International Hospital in Tokyo, Japan. The QI activity for in-patient falls prevention consisted of: 1) the fall risk assessment tool, 2) an intervention protocol to prevent in-patient falls, 3) specific environmental safety interventions, 4) staff education, and 5) multidisciplinary healthcare staff compliance monitoring and feedback mechanisms.The overall fall rate was 2.13 falls per 1000 patient days (350/164331) in 2004 versus 1.53 falls per 1000 patient days (263/172325) in 2010, representing a significant decrease (p?=?0.039). In the first 6?months, compliance with use of the falling risk assessment tool at admission was 91.5% in 2007 (3998/4368), increasing to 97.6% in 2010 (10564/10828). The staff compliance rate of implementing an appropriate intervention plan was 85.9% in 2007, increasing to 95.3% in 2010.In our study we observed a substantial decrease in patient fall rates and an increase of staff compliance with a newly implemented falls prevention program. A systematized QI approach that closely involves, encourages, and educates healthcare staff at multiple levels is effective.
Quantification of BCR-ABL mRNA in Plasma/Serum of Patients with Chronic Myelogenous Leukemia
Miwako Narita, Anri Saito, Aya Kojima, Minami Iwabuchi, Naoya Satoh, Takayoshi Uchiyama, Akie Yamahira, Tatsuo Furukawa, Hirohito Sone, Masuhiro Takahashi
International Journal of Medical Sciences , 2012,
Abstract: Quantification of tumor-associated mRNA extracted from blood cells/tissues containing tumor cells is used for evaluation of treatment efficacy or residual tumor cell burden in tumors including leukemia. However, this method using tumor cell-containing blood/tissue is difficult to evaluate the whole tumor cell burden in the body. In order to establish an efficient method to evaluate the whole tumor cell burden in the body, we tried to quantify tumor-associated mRNA existing in plasma/serum instead of leukemia cell-containing blood cells in patients with chronic myelogenous leukemia (CML) and compared the levels of BCR-ABL mRNA between plasma/serum and peripheral blood cells. mRNA of BCR-ABL, WT1 or GAPDH (control molecule) was detected by real-time RT-PCR using RNA extracted from plasma/serum of almost all the patients with CML. Copy numbers of BCR-ABL mRNA were significantly correlated between plasma/serum and peripheral blood cells. However, levels of BCR-ABL mRNA extracted from serum were low compared with those extracted with peripheral blood cells. The present findings suggest that although real-time RT-PCR of mRNA existing in plasma/serum could be used for evaluating the whole tumor cell burden in the body, it's required to establish an efficient method to quantify plasma/serum mRNA by nature without degrading during the procedure.
Page 1 /4006
Display every page Item

Copyright © 2008-2017 Open Access Library. All rights reserved.