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Search Results: 1 - 10 of 6509 matches for " Atala Anthony "
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Founding Editorial
Anthony Atala
The Scientific World Journal , 2004, DOI: 10.1100/tsw.2004.104
Tissue engineering, stem cells, cloning, and parthenogenesis: new paradigms for therapy
Hipp Jason,Atala Anthony
Journal of Experimental and Clinical Assisted Reproduction (JECAR) , 2004, DOI: 10.1186/1743-1050-1-3
Abstract: Background Patients suffering from diseased and injured organs may be treated with transplanted organs. However, there is a severe shortage of donor organs which is worsening yearly due to the aging population. Scientists in the field of tissue engineering apply the principles of cell transplantation, materials science, and bioengineering to construct biological substitutes that will restore and maintain normal function in diseased and injured tissues. Both therapeutic cloning (nucleus from a donor cell is transferred into an enucleated oocyte), and parthenogenesis (oocyte is activated and stimulated to divide), permit extraction of pluripotent embryonic stem cells, and offer a potentially limitless source of cells for tissue engineering applications. The stem cell field is also advancing rapidly, opening new options for therapy. The present article reviews recent progress in tissue engineering and describes applications of these new technologies that may offer novel therapies for patients with end-stage organ failure.
Steve J. Hodges,David Werle,Gordon McLorie,Anthony Atala
The Scientific World Journal , 2010, DOI: 10.1100/tsw.2010.54
Posterior Urethral Valves
Steve J. Hodges,Bhavin Patel,Gordon McLorie,Anthony Atala
The Scientific World Journal , 2009, DOI: 10.1100/tsw.2009.127
MYC Gene Delivery to Adult Mouse Utricles Stimulates Proliferation of Postmitotic Supporting Cells In Vitro
Joseph C. Burns, James J. Yoo, Anthony Atala, John D. Jackson
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048704
Abstract: The inner ears of adult humans and other mammals possess a limited capacity for regenerating sensory hair cells, which can lead to permanent auditory and vestibular deficits. During development and regeneration, undifferentiated supporting cells within inner ear sensory epithelia can self-renew and give rise to new hair cells; however, these otic progenitors become depleted postnatally. Therefore, reprogramming differentiated supporting cells into otic progenitors is a potential strategy for restoring regenerative potential to the ear. Transient expression of the induced pluripotency transcription factors, Oct3/4, Klf4, Sox2, and c-Myc reprograms fibroblasts into neural progenitors under neural-promoting culture conditions, so as a first step, we explored whether ectopic expression of these factors can reverse supporting cell quiescence in whole organ cultures of adult mouse utricles. Co-infection of utricles with adenoviral vectors separately encoding Oct3/4, Klf4, Sox2, and the degradation-resistant T58A mutant of c-Myc (c-MycT58A) triggered significant levels of supporting cell S-phase entry as assessed by continuous BrdU labeling. Of the four factors, c-MycT58A alone was both necessary and sufficient for the proliferative response. The number of BrdU-labeled cells plateaued between 5–7 days after infection, and then decreased ~60% by 3 weeks, as many cycling cells appeared to enter apoptosis. Switching to differentiation-promoting culture medium at 5 days after ectopic expression of c-MycT58A temporarily attenuated the loss of BrdU-labeled cells and accompanied a very modest but significant expansion of the sensory epithelium. A small number of the proliferating cells in these cultures labeled for the hair cell marker, myosin VIIA, suggesting they had begun differentiating towards a hair cell fate. The results indicate that ectopic expression of c-MycT58A in combination with methods for promoting cell survival and differentiation may restore regenerative potential to supporting cells within the adult mammalian inner ear.
GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes
Shi-Jiang Lu, Jennifer A Hipp, Qiang Feng, Jason D Hipp, Robert Lanza, Anthony Atala
Genome Biology , 2007, DOI: 10.1186/gb-2007-8-11-r240
Abstract: Large-scale transcriptional analysis was performed at distinct time points of hESC differentiation (undifferentiated hESCs, embryoid bodies, and blast cells, the last of which generates both hematopoietic and endothelial progenies). Identifying genes enriched in blast cells relative to hESCs revealed a genetic signature indicative of erythroblasts, suggesting that erythroblasts are the predominant cell type in the blast cell population. Because of the heterogeneity of blast cells, numerous comparisons were made to publicly available data sets in silico, some of which blast cells are capable of differentiating into, to assess and characterize the blast cell population. Biologically relevant comparisons masked particular genetic signatures within the heterogeneous population and identified genetic signatures indicating the presence of endothelia, cardiomyocytes, and hematopoietic lineages in the blast cell population.The significance of this microarray study is in its ability to assess and identify cellular populations within a heterogeneous population through biologically relevant in silico comparisons of publicly available data sets. In conclusion, multiple in silico comparisons were necessary to characterize tissue-specific genetic signatures within a heterogeneous hemangioblast population.The establishment of human embryonic stem cells (hESCs) raised the possibility of being able to treat/cure many human diseases that are nowadays untreatable. This therapeutic potential, however, largely relies on the efficient and controlled differentiation of hESCs towards a specific cell type and the generation of homogeneous cell populations. Many differentiation protocols utilize the formation of progenitors through a stepwise approach. Thus, characterizing and understanding mixed populations of progenitor stages will be of increasing importance in stem cell research.hESCs have been shown to be able to differentiate into a variety of cell types, including hematopoietic precur
Water transport and gas exchange in the non-vascular plant Dendroligotrichum dendroides (Brid. ex Hedw.)Broth. (Polytrichaceae, Bryophyta)
Gayana. Botánica , 2011, DOI: 10.4067/S0717-66432011000100008
Abstract: se midió la conductancia hidráulica específica (ks) y el intercambio de gases en individuos de d. dendroides (polytrichaceae, musci). ks fue más alta que en algunas coníferas y comparable con algunas angiospermas le?osas, pero la fotosíntesis (amax) fue relativamente baja. los resultados muestran que una planta no vascular puede alcanzar altos valores de ks, sugiriendo un funcionamiento "vascular" similar a las traqueófitas.
Water transport and gas exchange in the non-vascular plant Dendroligotrichum dendroides (Brid. ex Hedw.)Broth. (Polytrichaceae, Bryophyta) Transporte de agua e intercambio de gases en la planta no vascular Dendroligotrichum dendroides (Brid. ex Hedw.)Broth. (Polytrichaceae, Bryophyta)
Cristian Atala
Gayana. Botanica , 2011,
Abstract: Se midió la conductancia hidráulica específica (Ks) y el intercambio de gases en individuos de D. dendroides (Polytrichaceae, Musci). Ks fue más alta que en algunas coníferas y comparable con algunas angiospermas le osas, pero la fotosíntesis (Amax) fue relativamente baja. Los resultados muestran que una planta no vascular puede alcanzar altos valores de Ks, sugiriendo un funcionamiento "vascular" similar a las traqueófitas.
Self-Renewal and Differentiation Capacity of Urine-Derived Stem Cells after Urine Preservation for 24 Hours
Ren Lang, Guihua Liu, Yingai Shi, Shantaram Bharadwaj, Xiaoyan Leng, Xiaobo Zhou, Hong Liu, Anthony Atala, Yuanyuan Zhang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0053980
Abstract: Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) after optimal preservation of urine specimens for up to 24 hours. A total of 415 urine specimens were collected from 12 healthy men (age range 20–54 years old). About 6×104 cells shed off from the urinary tract system in 24 hours. At least 100 USC clones were obtained from the stored urine specimens after 24 hours and maintained similar biological features to fresh USCs. The stored USCs had a “rice grain” shape in primary culture, and expressed mesenchymal stem cell surface markers, high telomerase activity, and normal karyotypes. Importantly, the preserved cells retained bipotent differentiation capacity. Differentiated USCs expressed myogenic specific proteins and contractile function when exposed to myogenic differentiation medium, and they expressed urothelial cell-specific markers and barrier function when exposed to urothelial differentiation medium. These data demonstrated that up to 75% of fresh USCs can be safely persevered in urine for 24 hours and that these cells stored in urine retain their original stem cell properties, indicating that preserved USCs could be available for potential use in cell-based therapy or clinical diagnosis.
Protective Effect of Human Amniotic Fluid Stem Cells in an Immunodeficient Mouse Model of Acute Tubular Necrosis
Laura Perin,Sargis Sedrakyan,Stefano Giuliani,Stefano Da Sacco,Gianni Carraro,Liron Shiri,Kevin V. Lemley,Michael Rosol,Sam Wu,Anthony Atala,David Warburton,Roger E. De Filippo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009357
Abstract: Acute Tubular Necrosis (ATN) causes severe damage to the kidney epithelial tubular cells and is often associated with severe renal dysfunction. Stem-cell based therapies may provide alternative approaches to treating of ATN. We have previously shown that clonal c-kitpos stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system. Herein, we show for the first time the successful therapeutic application of hAFSC in a mouse model with glycerol-induced rhabdomyolysis and ATN. When injected into the damaged kidney, luciferase-labeled hAFSC can be tracked using bioluminescence. Moreover, we show that hAFSC provide a protective effect, ameliorating ATN in the acute injury phase as reflected by decreased creatinine and BUN blood levels and by a decrease in the number of damaged tubules and apoptosis therein, as well as by promoting proliferation of tubular epithelial cells. We show significant immunomodulatory effects of hAFSC, over the course of ATN. We therefore speculate that AFSC could represent a novel source of stem cells that may function to modulate the kidney immune milieu in renal failure caused by ATN.
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