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Search Results: 1 - 10 of 68 matches for " Asmita Prabhune "
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Gold Nanoparticles for Colorimetric detection of hydrolysis of antibiotics by penicillin G acylase  [PDF]
Neha R. Tiwari, Ambrish Rathore, Asmita Prabhune, Sulabha K. Kulkarni
Advances in Bioscience and Biotechnology (ABB) , 2010, DOI: 10.4236/abb.2010.14042
Abstract: A simple inexpensive method of monitoring hydrolysis of an antibiotic penicillin G (pen G) and subsequent enzyme detection using gold nanoparticles is presented. Gold nanoparticles capped with Cetyl trimethyl ammonium bromide (CTAB) are synthesized using chemical route. The particles could be used for detection of Penicillin G acylase (PGA) enzyme by incorporating hydrolysis reaction with pen G. This hydrolysis reaction leads to a shift in the surface plasmon band of gold nanoparticles from 527 nm to 545 nm accompanied by a visual colorimetric change in the solution from red to blue. The process is attributed to aggregation of nanoparticles caused due to displacement of CTAB bilayer by byproducts of the hydrolysis reaction. It is proposed that the presence of 0.007 mg/ml of PGA can be detected by a color change of gold nanoparticles solution without requiring any complicated instrument or highly trained operator to conduct the test. The method could also identify the presence of different penicillins by showing different spectral shifts. Thus the work presented here would be useful not only for the detection of the pharmaceutically important drug Pen G, but also represents a general methodology for the detection of enzymes, eg PGA.
Jatropha Oil Derived Sophorolipids: Production and Characterization as Laundry Detergent Additive
Kasturi Joshi-Navare,Poonam Khanvilkar,Asmita Prabhune
Biochemistry Research International , 2013, DOI: 10.1155/2013/169797
Abstract: Sophorolipids (SLs) are glycolipidic biosurfactants suitable for various biological and physicochemical applications. The nonedible Jatropha oil has been checked as the alternative raw material for SL synthesis using C. bombicola (ATCC22214). This is useful towards lowering the SL production cost. Through optimization of fermentation parameters and use of resting cell method, the yield 15.25?g/L could be achieved for Jatropha oil derived SL (SLJO) with 1% v/v oil feeding. The synthesized SL displayed good surfactant property. It reduced the surface tension of distilled water from 70.7?mN/m to 33.5?mN/m with the Critical Micelle Concentration (CMC) value of 9.5?mg/L. Keeping the prospective use of the SL in mind, the physicochemical properties were checked along with emulsion stability under temperature, pH stress, and in hard water. Also antibacterial action and stain removal capability in comparison with commercial detergent was demonstrated. SLJO enhanced the detergent performance. Based on the results, it can be said that SLs have utility as fabric cleaner with advantageous properties such as skin friendly nature, antibacterial action, and biodegradability. Therefore SLs are potential green molecules to replace synthetic surfactants in detergents so as to reduce harm caused to environment through detergent usage. 1. Introduction In terms of production volume, surfactants belong to the most important classes of industrial chemicals with a current total world production exceeding 13 million tonnes per year [1]. About half that volume is used in household and laundry detergents and the other half in a wide variety of industrial sectors, particularly the chemical, textile, food, and paper industry, cosmetics, personal, and health care, agriculture, and so forth. The majority of the currently used surfactants are petroleum-based and are produced by chemical means. These compounds are often toxic to the environment, and their use may lead to significant ecological problems, particularly in washing applications as these surfactants inevitably end up in the environment after use [2]. The ecotoxicity, bioaccumulation, and biodegradability of surfactants are therefore issues of increasing concern. Phosphates are being recognized as one of the essential nutrients contributing to the eutrophication and detergents are one of the many sources of phosphate discharged to the environment [3]. Therefore attempts should be made to reduce the detergent load in to the environment. In this scenario the biosurfactants are promising alternatives to synthetic surfactants as
Ameliorative potentials of Syzygium jambolanum extract against arsenic-induced stress in L6 cells in vitro
Asmita Samadder
Zhong Xi Yi Jie He Xue Bao , 2012,
Abstract: OBJECTIVE: To determine the ameliorative potentials of Syzygium jambolanum (SJ) extract in L6 skeletal muscle cells in regard to arsenic-induced impairment of optimum glucose homeostasis and improper functioning of mitochondria.METHODS: Several study parameters like glucose level and mitochondrial functioning through indexes of pyruvate kinase, glucokinase and mitochondrial membrane potential were assessed. The expression of the relevant marker proteins and mRNAs like glucose transporter 4 (GLUT4), insulin receptor substrate 1 (IRS1), IRS2 and glucokinase for tracking down the signalling cascade were critically analyzed.RESULTS: Introduction of SJ extract could bring about positive modulation of various markers, by acting on GLUT4, thereby bringing about an attenuation of the arsenite-induced toxic conditions in L6 cells.CONCLUSION: Syzygium jambolanum extract has considerable ameliorating potentials against arsenic-induced glucose imbalance and stress and has possibility of therapeutic use in the management of arsenic-induced toxicity including hyperglycemia.
Conservation-induced displacement: A comparative study of two Indian protected areas
Kabra Asmita
Conservation & Society , 2009,
Abstract: Attempts at ′preservation via displacement′ are an extreme manifestation of the ′fortress′ or an exclusionary conservation paradigm, support for which has increased lately due to escalating conservation threats. While the policies and processes emanating from this paradigm have produced positive conservation outcomes for some Protected Areas, livelihood outcomes for the displaced people have seldom been as positive. This article examines whether the impoverishment risks arising from conservation-induced displacement tend to vary with the degree of marginalisation of the displaced community. In this light, this article examines in detail the impact on livelihood of conservation-induced displacement in two Protected Areas (PAs) of India. The article posits that understanding the dynamic livelihood context of displaced communities, especially the ecological base of their livelihoods, is critical to any assessment of their pre- and post-displacement livelihood strategies and livelihood outcomes (such as income, poverty, food security and health). A variety of livelihood parameters, including compensation received, consumption flows, agricultural production, monetary income, food security, headcount ratio of poverty and overall poverty indices have been studied, to understand the extent to which key livelihood risks arising out of displacement are addressed by the rehabilitation package and process in the two PAs. The Sahariya is a forest-dependent Adivasi community living in and around the Kuno Wildlife Sanctuary in the semi-arid tropical region of Madhya Pradesh. The Sahariya Adivasis of the Kuno Sanctuary were a socially, politically and economically marginalised community, whose lives and livelihoods were intricately linked to their ecological base. We found that inadequate attention was paid to this factor while designing and implementing a suitable rehabilitation package for the 1650 Sahariya households displaced from this PA. As a result, their material condition deteriorated after displacement, due to loss of livelihood diversification opportunities and alienation from their natural resource base. Displacement thus resulted in rapid proletarianisation and pauperisation of these households, and their ′integration′ into the national ′mainstream′ occurred at highly disadvantageous terms. The 430 odd households displaced from the Bhadra Wildlife Sanctuary in the Western Ghats (a biodiversity hotspot in the Southern Indian state of Karnataka) consisted of relatively less marginalised social groups like the Gowdas and the Shettys, both of whom occupy a prom
Power Corrections in Deep Inelastic Scattering and Non-perturbative Light-front Dynamics
Asmita Mukherjee
Physics , 2001,
Abstract: In this thesis, we have investigated the higher twist structure functions in the recently developed method based on light-front Hamiltonian QCD. Because of various special properties of light-front QCD, this is a more intuitive approach towards deep inelastic scattering but has a well defined field theoretic calculational procedure. Because our method is different from the conventional one, we have obtained various new results. Also, it is easier to explore many aspects which are difficult to understand in the conventional way. The structure functions, twist four $F_L$ and $g_T$ contain non-trivial interaction dependence in the operator structure and therefore involve quark-gluon dynamics. We have shown that the intergrals of these are directly related to the light-front Hamiltonian density and transverse spin operator respectively. These are non-perturbative relations. We have also renormalized the twist four longitudinal structure function and the full transverse spin operator respectively upto one loop in light-front Hamiltonian perturbation theory. We have investigated twist four $F_L$ for bound states like a meson in 1+1 dimensional QCD and for a positronium in 3+1 dimensional QED.
A Field Theoretic Investigation of Spin in QCD
Asmita Mukherjee
Physics , 2000, DOI: 10.1016/S0920-5632(00)00867-7
Abstract: Utilizing the kinematical boost in light-front formalism one can address the issue of relativistic spin operators in an arbitrary reference frame. In the gauge $A^+=0$, the interaction dependent transverse spin operators can be separated into three parts. In analogy with the helicity sum rule, we propose a transverse spin sum rule. We perform a one loop renormalization of the transverse spin operator and show that the counterterm needed is the same as the linear mass counterterm in the light-front QCD Hamiltonian.
Twist Four Longitudinal Structure Function for a Positronium-like Bound State in Light-Front QED
Asmita Mukherjee
Physics , 2001, DOI: 10.1016/S0370-2693(01)00954-6
Abstract: To have an analytic understanding of the higher-twist structure functions, we calculate twist four longitudinal structure function for a positronium-like bound state in weak coupling light-front QED. We find that in the weakly coupled system, the fermionic part of $F_L$ is related to the kinetic energy of the fermions and not to the interaction. We verify a previously proposed sum rule in this limit, which in this case reduces to a relation connecting the kinetic and the potential energies to the binding energy of positronium. Using the analytic form of the wave function of positronium in this limit, we show that the constituent counting rule does not hold for $x \to 1$. The twist four $F_L$ in this limit is similar in form to a widely used phenomenological ansatz.
Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro
Meenakshi Prabhune,Florian Rehfeldt,Christoph F. Schmidt
Physics , 2015,
Abstract: Microtubule structure and functions have been widely studied in vitro and in cells. Research has shown that cysteines on tubulin play a crucial role in the polymerization of microtubules. Here, we show that blocking sulfhydryl groups of cysteines in taxol-stabilized polymerized microtubules with a commonly used chemical crosslinker prevents temporal end-to-end annealing of microtubules in vitro. This can dramatically affect the length distribution of the microtubules. The crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, sulfo-SMCC, consists of a maleimide and a N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Interestingly, addition of a maleimide dye alone does not show the same prevention of annealing in stabilized microtubules. This study shows that the sulfhydryl groups of cysteines of tubulin that are vital for the polymerization are also important for the subsequent annealing of microtubules.
Extending cell cycle synchrony and deconvolving population effects in budding yeast through an analysis of volume growth with a structured Leslie model  [PDF]
Chris C. Stowers, Asmita M. Boczko
Journal of Biomedical Science and Engineering (JBiSE) , 2010, DOI: 10.4236/jbise.2010.310129
Abstract: Budding yeast are a fundamental organism at the center of systems biology research. Understanding the physiology and kinetics of their growth and division is fundamental to the design of models of gene regulation and the interpretation of experimental measurements. We have developed a Leslie model with structured volume and age classes to understand population growth and cell cycle synchrony in budding yeast. The model exhibits broad agreement with a variety of experimental data. The model is easily annotated with volume milestones and cell cycle phases and at least three distinct goals are realizable: 1) One can investigate how any single cell property manifests itself at the population level. 2) One can deconvolve observed population averages into individual cell signals structured by volume and age. 3) One can investigate controllability of the population dynamics. We focus on the latter question. Our model was initially designed to answer the question: Can continuous volume filtration extend synchrony? To date, most general experimental methods can produce an initially synchronous population whose synchrony decays rapidly over three or four cell cycles. Our model predicts that continuous volume filtration can extend this maintenance of synchrony by an order of magnitude. Our data inform the development of simple fluidic devices to extend synchrony in continuous culture at all scales from nanophysiometers to bioreactors.
Trend and Periodicity Analysis in Rainfall Pattern of Nira Basin, Central India  [PDF]
Asmita Ramkrishna Murumkar, Dhyan Singh Arya
American Journal of Climate Change (AJCC) , 2014, DOI: 10.4236/ajcc.2014.31006
Abstract:

Seasonal and annual rainfall data of the stations: Akluj, Baramati, Bhor and Malsiras stations located in Nira Basin, Central India, were analyzed for studying trend and periodicity using 104 years’ rainfall data. The analysis was carried out by using Mann-Kendall (MK), Modified Mann-Kendall (MMK) and Theil and Sen’s slope estimator tests describing rising trend at all the stations. However, it is statistically significant at Akluj and Bhor stations at 10% significance level. Bhor station showed the maximum increase in percentage change i.e. 0.28% in annual rainfall. Monsoon and post-monsoon seasonal rainfall shows a rising trend while the summer and winter seasonal rainfall shows a falling trend. Wavelet analysis showed prominent annual rainfall periods ranging from 2 to 8 years at all the stations after 1960s resulting in describing more changes in the rainfall patterns after 1960s.

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