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Search Results: 1 - 10 of 3074 matches for " Aparna Chandra Sekhar "
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Isolation and Identification of Shoot-Tip Associated Endophytic Bacteria from Banana cv. Grand Naine and Testing for Antagonistic Activity against Fusarium oxysporum f. sp. cubense  [PDF]
Aparna Chandra Sekhar, Pious Thomas
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.67101
Abstract: Endophytic bacteria colonizing the shoot-tips of banana cv. Grand Naine were isolated and tested for the antagonistic activity against the Panama wilt pathogen Fusarium oxysporum f. sp. cubense (Foc). Pre-isolation, the suckers were given extensive disinfection treatments and the homogenate from the excised shoot-tip portion was plated on nutrient agar (NA) and trypticase soy agar (TSA). This yielded altogether 47 isolates: 26 on NA and 21 on TSA, respectively, from the 10 suckers collected during August to February. The number of bacterial isolates obtained per sucker varied from one to 15 based on colony characteristics registering up to 10 distinct species per shoot-tip based on 16S rRNA sequence analysis. The 47 isolates belonged to 19 genera and 25 species under the phylogenetic classes of Actinobacteria, α- and γ-Proteobacteria and Firmicutes. Actinobacteria constituted the predominant phylum (55% isolates) with the constituent genera of Arthrobacter, Brevibacterium, Corynebacterium, Curtobacterium, Kocuria, Kytococcus, Micrococcus, Naumanella, Rothia and Tessaracoccus spp. and an unidentified isolate belonging to the family Frankiaceae. Proteobacteria constituted the second major phylum (Brevundimonas, Enterobacter, Klebsiella, Pseudomonas, Serratia and Sphingomonas spp.) followed by Firmicutes (Bacillus and Staphylococcus spp.). Antagonistic activity of the endophytes against Foc was tested through agar plate assays (pit and spot applications on fungal lawn) employing potato dextrose agar and NA. Endophytic Pseudomonas aeruginosa (isolate GNS.13.2a) which was associated with a single sucker showed significant growth
Statistical Inference for a two station tandem queue with atmost one customer to wait between the stations
Vaidya Nathan,Chandra Sekhar
Pakistan Journal of Statistics and Operation Research , 2012, DOI: 10.1234/pjsor.v8i2.338
Abstract: A maximum likelihood estimator (MLE), a consistent asymptotically normal (CAN) estimator and asymptotic confidence limits for the expected number of customers in the system for a sequential two station, single server system with Poisson input and exponential service, where no queue is allowed in front of station 1 and atmost one customer is allowed to wait between the stations and with blocking are obtained.
Practical Approach for Master Data Management
Chandra Sekhar Bhagi
World of Computer Science and Information Technology Journal , 2011,
Abstract: Any organization typically has data on Customers, Financials (Chart of accounts, profits, Cost), Products, Business Partners (Suppliers, Distributors), Employees, Locations, Sales Contacts, Physical assets, Claims or Policies (Insurance). These data items or business entities are referred as Master Data. The process and technology involved in acquiring Master Data from multiple domains across enterprise and thereby maintaining a single consistent view is Master Data Management (MDM). MDM is getting popularity in Health & Life sciences, Medical Device Manufacturing, Financial Services, Insurance, Manufacturing & Technology, Retail, Consumer Packaged Goods, Telecommunications, Information services & Media, Aerospace, Defense and Government. The current article illustrates the technical solution to implement enterprise Master Data Management in domain specific companies.
Analysis of Transaction and Concurrency Mechanism in Two Way Waiting Algorithm for different Databases
International Journal of Computer Science and Communication Networks , 2012,
Abstract: Database is the well-organized collection of data in a meaningful way that user can perform the transaction. Transaction is a sequence of many actions considered to be atomic. In the coming months or years you will probably be implementing applications for multiple users to use across a network. Client/server environments are designed specifically for this purpose. Traditionally, a server (in this case, a database server) supports multiple network connections to it. As often happens with technology, this newfound flexibility adds a new degree of complexity to the environment. The throughput of the transactions can be increased by performing the transactions in parallel. Transactions are performed in parallel without infringing the data integrity. When the transactions are executing in parallel then this will lead to concurrency in database and deadlock of the transaction. This work will analyze how and is done to prevent the occurrence of deadlock and also improves system performance by shrink the number of restarts transaction which also saves the time and cost for performing the transaction in the system. So, it improves the efficiency of the distributed system.
Chandra Sekhar.k*
International Journal of Pharmacy and Technology , 2010,
Abstract: Background: The Government of India, WHO and World Bank together reviewed the NTP in the year 1992. The revised strategy was introduced in the country as a Pilot Project since 1993 in a phasedmanner as Pilot Phase I, Pilot Phase II and Pilot Phase III. By the end of 1998, only 2 per cent of the total population of India was covered by RNTCP.Objectives:1. To know the sputum conversion rate among the sputum positive tuberculosis patients.2. To find the treatment outcome of smear positive tuberculosis patients.Methodology: This Hospital based descriptive study was carried out among the out patients attending Medicine, Surgery, OBG, ENT, Dermatology, TB and Chest Department of MNR Medical College during the period of January 1st to December 2006. Oral questionnaire prepared and applied to all the individuals (377 TB suspects) those who visited for sputum examination at DMC.Results: Out of 138 TB Cases 67.3% cases were pulmonary and 32.7% were extra pulmonary. Out of 58 sputum positive cases, 49 cases returned for sputum examination at successive months and rest were transfer out. Of these 49 cases, 46 cases have converted negative at 2, 4, 6 months respectively and remaining 3 cases had sputum conversion at 3,5, 7 months. Tuberculosis prevalence increased withage i.e. above 15 years of age group and significant association was found (P< 0.001). HIV- TB Co-Infection was found in 2 cases (1.4%) of the total TB (138) cases.Conclusions: Out of 58 sputum positive cases, 4 cases (4/58) not traced and 5 cases (5/58) transferred out to neibouring districts. There is a need to increase RNTCP network to minimize the dropouts.
Advanced Secure Dynamic-ID Based Remote User Authentication Scheme
chandra sekhar vorugunti
Journal of Global Research in Computer Science , 2012,
Abstract: With large scale deployment of E-Commerce applications, security of data over insecure communication channels becomes crucial challenge. To ensure authenticity of a remote user to access server resources, smart card based remote user authentication schemes have been widely adopted. In 2004, Das et al [9] proposed first of its kind of protocol for remote user authentication with smart cards using Dynamic Id to protect user anonymity. Various researchers like Awasthi and lal[4] had pointed various issues in Das et al scheme. They had shown that Das et al scheme is completely insecure and it is equivalent to open server access without password. In 2006 Bindu et al[13] claimed that Das et al scheme doesn’t provides mutual authentication and various other issues. Bindu et al proposed an improved scheme which overcomes the weaknesses of Das et al scheme and achieves mutual authentication. In this paper we will show that Bindu et al scheme cannot achieve Mutual authentication under their assumption. In addition their scheme is vulnerable to user-impersonation attack, server-masquerading attack, Man in the Middle attack, password guessing attack, DoS attack, Revealing of server secret information to users and fails to preserve user anonymity which is most important requirement for E-Commerce applications. We then present our improved scheme to overcome the vulnerabilities stated in Bindu et al scheme while preserving all the merits of their scheme.
Journal of Science and Arts , 2012,
Abstract: n this note, we have obtained a novel result which is stated in the form of a theorem on mixed trilateral generating relations involving modified Laguerre polynomials. As a particular case, we obtain an interesting result, which is worthy of notice.
Influence of Bavistin, Cefotoxime, Kanamycin and Silver Thiosulphate on Plant Regeneration of Solanum viarum (Dunal)—An Important Anticancer Medicinal Plant  [PDF]
M. D. Mahadev, Chandra Sekhar Panathula, C. V. Naidu
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.53053

The effects of bavistin, cefotoxime and kanamycin along with the ethylene inhibitor silver thiosulphate on in vitro regeneration using the axillary buds of Solanum viarum (D.) have been investigated. Axillary bud explants when cultured on MS medium supplemented with bavistin (100 mg/l) maximum number of shoots (4.21 ± 0.83) were observed. In a separate experiment, bavistin along with growth regulators such as BAP and TDZ was added to MS medium containing sucrose (3%). Maximum number of shoots was obtained (5.55 ± 0.28) in MS medium supplemented with bavistin (100 mg/l) and BAP (2.0 mg/l). Cefotoxime and kanamycin (10-150 μM/L) were added to MS medium supplemented with BAP (2.0 mg/l) with sucrose (3%) having shown the maximum shoot regeneration (4.38 ±

Impact of Different Carbohydrates and Their Concentrations on in Vitro Regeneration of Solanum viarum (Dunal)—An Important Anticancer Medicinal Plant  [PDF]
Manikyam Doraiswamy Naidu Mahadev, Chandra Sekhar Panathula, ChallagundlaVaradarajulu Naidu
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.51026

In this present study, the effect of various carbon sources such as sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used. Among the different concentrations (1%-6%) of carbohydrates studied, the maximum number of shoots (22.6 ± 0.50) and shoot length (5.92 ± 0.13 cm) was obtained on MS medium supplemented with 4% (w/v) fructose. The least number of shoots (1.5 ± 0.32) was obtained on 6% maltose and the least shoot length (1.2 ± 0.23) was observed on 6% glucose. Among the four types of carbon sources that were employed in the present study, fructose at 4%

The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on In Vitro Plant Regeneration of Centella asiatica (L.)—An Important Antijaundice Medicinal Plant  [PDF]
Chandra Sekhar Panathula, Manikyam Doraiswamy Naidu Mahadev, Challagundla Varadarajulu Naidu
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.53038

Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 μM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 μM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0

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