oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

3 ( 1 )

2019 ( 44 )

2018 ( 72 )

2017 ( 95 )

Custom range...

Search Results: 1 - 10 of 43904 matches for " Antonio; Santiesteban Torres "
All listed articles are free for downloading (OA Articles)
Page 1 /43904
Display every page Item
Evaluación bioquímico-inmunológica de infectados crónicos por el virus de la hepatitis B: Comportamiento de la respuesta inmune en sus contactos familiares
Santiesteban Torres,Jorge Arturo; Alerm González,Alina; González Griego,Antonio;
Revista Cubana de Investigaciones Biom??dicas , 2000,
Abstract: chronic infection by hbv is a clear and important cause of morbidity and mortality worldwide. an important problem, for the control and eradication of this disease is the existance of chronic hbv carriers and their family contacts. a biochemical and immunological study of chronic hbv reservoirs were conducted to determine the relation between the concentrations of hbv surface antigen, detected and quantified by the elisa method, and the serum viral dna concentrations; as well as the correlation between the levels of antigenemia and the transaminase values (alat). a direct linking was proved between viral dna and hbsag levels and also between antigen concentration and alat values. a significant percent of carriers with high viral charges and normal enzymatic levels was observed. the performance of immune response to hbv in those persons living with the chronic hbv-infested subjects was analyzed and the frequency of occurence of immune, sensitive and infected persons was estimated resulting in a high number of infected persons and persons living with the chronic patients without protection. qualitative and quantitative elisa were used to assess the response of antibodies to hbsag, induced by vaccines or by normal contacts of blood related and non-related persons in the family, but there was no difference among them. the hbsag concentration of the carrier was associated with levels of antibodies to hbsag; after completion of the vaccination program in blood related and non-related subjects, then the association was found to be negative in the former and the existance of shared gens linked to the control of anti-hbsag response and viral clearing were infered
Redise?o y estandarización de un método inmunoenzimático para detectar anticuerpos anti-estreptoquinasa en muestras de suero humano
López Matilla,Lien; González Griego,Antonio; Santiesteban Torres,Jorge Arturo; Alerm González,Alina;
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: a sandwich, heterogenous, qualitative, doble antigen immunoenzimatic method was standardized to detect anti-streptokinase antibodies in pure samples of human serum. different concentrations of the recovering antigen and conjugate dilutions, as well as various times and temperatures of incubation of the samples were assayed. the cut-off value of the assay and its detection limit were established and it was proved if there were some unspecificities. 0.1 mg/ml was determined as an optimal recovering concentration for a conjugate dilution of 1:100 000. the optimum conditions of incubation of the samples were 30 min at lab temperature and there were no interferences of other analytes with the technique.the optical densities between 0.282 and 0.654 were established as normal values of the technique.
Estudio del transporte madre-hijo de anticuerpos antiestreptoquinasa
Lien López Matilla,Antonio González Griego,Alina Alerm González,Arturo Santiesteban Torres
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: Se estudió cómo se comporta el transporte de anticuerpos anti-estreptoquinasa entre la madre y el recién nacido y cuáles son los factores que influyen en este. Se colectaron 125 muestras sanguíneas de puérperas, por la vena antecubital y de sus respectivos hijos a partir del cordón umbilical. Se confeccionaron encuestas para la recolección de datos a partir de las historias clínicas de las madres y los recién nacidos. Se empleó un método inmunoenzimático, heterogéneo, cualitativo, tipo sandwich de doble antígeno para la detección de anticuerpos anti-estreptoquinasa. A partir de los resultados obtenidos se concluyó que existe un transporte activo de los anticuerpos anti-estreptoquinasa de la madre al feto y que este transporte se ve influenciado por la edad cronológica de la madre, la edad gestacional, el estado nutricional de la madre y el peso del recién nacido. The behavior of the transport of streptokinase antibodies from the mother to the newborn infant, as well as the factors influencing on it, were studied here. 125 blood samples were obtained from the antecubital vein of the puerperas and from the umbilical cord of their children. Surveys were done to collect data from the medical histories of the mothers and the newborn infants. An immunoenzimatic, heterogenous and qualitatitve sandwich doble antigen method was used to detect anti-streptokinase antibodies. According to the results, it was concluded that there is an active transport of anti-streptokinase antibodies from the mother to the fetus and that this transport is influenced by the chronological age of the mother, the gestational age, the mother’s nutritional status and the birth weight.
Redise o y estandarización de un método inmunoenzimático para detectar anticuerpos anti-estreptoquinasa en muestras de suero humano
Lien López Matilla,Antonio González Griego,Jorge Arturo Santiesteban Torres,Alina Alerm González
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: Se procedió a estandarizar un método inmunoenzimático, heterogéneo, cualitativo, tipo sandwich de doble antígeno para la detección de anticuerpos contra la estreptoquinasa en muestras puras de suero humano. Se ensayaron diferentes concentraciones del antígeno de recubrimiento y diluciones del conjugado, así como diferentes tiempos y temperaturas de incubación de las muestras. Se estableció el valor de corte del ensayo y su límite de detección y se comprobó si existían inespecificidades. Se determinó como concentración óptima de recubrimiento 0,1 μg/mL para una dilución del conjugado de 1:100 000. Las condiciones óptimas de incubación de las muestras fueron 30 min a temperatura de laboratorio y no se comprobaron interferencias de otros analitos con la técnica. Se establecieron como valores normales de la técnica las densidades ópticas que se encuentran entre 0,282 y 0,654. A sandwich, heterogenous, qualitative, doble antigen immunoenzimatic method was standardized to detect anti-streptokinase antibodies in pure samples of human serum. Different concentrations of the recovering antigen and conjugate dilutions, as well as various times and temperatures of incubation of the samples were assayed. The cut-off value of the assay and its detection limit were established and it was proved if there were some unspecificities. 0.1 mg/mL was determined as an optimal recovering concentration for a conjugate dilution of 1:100 000. The optimum conditions of incubation of the samples were 30 min at lab temperature and there were no interferences of other analytes with the technique.The optical densities between 0.282 and 0.654 were established as normal values of the technique.
Evaluación bioquímico-inmunológica de infectados crónicos por el virus de la hepatitis B: Comportamiento de la respuesta inmune en sus contactos familiares
Jorge Arturo Santiesteban Torres,Alina Alerm González,Antonio González Griego
Revista Cubana de Investigaciones Biom??dicas , 2000,
Abstract: La infección crónica por el virus de la hepatitis B (VHB) es una clara e importante causa de morbilidad y mortalidad en el mundo. Un importante problema para el control y la erradicación de la enfermedad es la exisniveles de anti-AgsHB, después del esquema completo de vacunación en consanguíneos y no consanguíneos, resultó ser de tipo negativa en los primeros e infirió la existencia de genes compartidos relacionados con el control de la respuesta anti-AgsHB y con el aclaramiento viral Chronic infection by HBV is a clear and important cause of morbidity and mortality worldwide. An important problem, for the control and eradication of this disease is the existance of chronic HBV carriers and their family contacts. A biochemical and immunological study of chronic HBV reservoirs were conducted to determine the relation between the concentrations of HBV surface antigen, detected and quantified by the ELISA method, and the serum viral DNA concentrations; as well as the correlation between the levels of antigenemia and the transaminase values (ALAT). A direct linking was proved between viral DNA and HbsAg levels and also between antigen concentration and ALAT values. A significant percent of carriers with high viral charges and normal enzymatic levels was observed. The performance of immune response to HBV in those persons living with the chronic HBV-infested subjects was analyzed and the frequency of occurence of immune, sensitive and infected persons was estimated resulting in a high number of infected persons and persons living with the chronic patients without protection. Qualitative and quantitative ELISA were used to assess the response of antibodies to HbsAg, induced by vaccines or by normal contacts of blood related and non-related persons in the family, but there was no difference among them. The HbsAg concentration of the carrier was associated with levels of antibodies to HbsAg; after completion of the vaccination program in blood related and non-related subjects, then the association was found to be negative in the former and the existance of shared gens linked to the control of anti-HbsAg response and viral clearing were infered
Estudio del transporte madre-hijo de anticuerpos antiestreptoquinasa
López Matilla,Lien; González Griego,Antonio; Alerm González,Alina; Santiesteban Torres,Arturo; águila Setién,Sonia; Wanton,Amada; Bancoul Moya,Edna; álvarez Díaz,María Herminia;
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: the behavior of the transport of streptokinase antibodies from the mother to the newborn infant, as well as the factors influencing on it, were studied here. 125 blood samples were obtained from the antecubital vein of the puerperas and from the umbilical cord of their children. surveys were done to collect data from the medical histories of the mothers and the newborn infants. an immunoenzimatic, heterogenous and qualitatitve sandwich doble antigen method was used to detect anti-streptokinase antibodies. according to the results, it was concluded that there is an active transport of anti-streptokinase antibodies from the mother to the fetus and that this transport is influenced by the chronological age of the mother, the gestational age, the mother?s nutritional status and the birth weight.
Estudio de seguridad, fase I de ensayo clínico, de la gammaglobulina hiperinmune anti-hepatitis B administrada unisitio y junto con la vacuna AgsHB recombinante: resultados preliminares
Acosta Acosta,Josué?; González Griego,Antonio; Caro Machado,Ramón; González Ramírez,Victoria E; García Castillo,Elsa; Santiesteban Torres,Jorge A; Alerm González,Alina; Montenegro,Adonis;
Revista Cubana de Investigaciones Biom??dicas , 2004,
Abstract: the vaccine preparation of the recombinant hbsag vaccine with the hyperimmune antihepatitis b gammaglobulin (passive immunization of antibodies) was used and the safety (phase i of clinical trials) of gammaglobulin was evaluated on being administered in one site together with the antihepatitis b vaccine. 25 sound volunteers aged 20-50 were immunized with the vaccine preparation of hyperimmune gammaglobulin (200 ui/ml) and with antihepatitis b vaccine (20 g hbsag/dose), which were administered in only one site and as a whole in the deltoid region in the time 0-1 month within the immunization scheme of the vaccine (0-1-3 months). no undesirable local or systemic effects were detected in any of the participants. the inocuity observed makes possible to go on with further phases of clinical studies
Expresión lingüística e identidad en los latinos de los Estados Unidos
Antonio Torres
Confluenze. Rivista di Studi Iberoamericani , 2009,
Abstract: This article analyses the historical and demographical circumstances of the population of Hispanic origin in the United States, this population’s use of Spanish, English, and also hybrid linguistic forms, as well as the literary reflection of mixed identities. We assert that, in this unstable situation, the conjunction of Spanish and English, resulting in infinite manifestations, is the channel for the emergence of aspects of identity and of means of expression which are applauded by some and combated by most, but which, irrespective of those reactions, are the unavoidable consequence of this linguistic and cultural contact Se analizan las circunstancias históricas y demográficas que se relacionan con la población de origen hispanohablante de los Estados Unidos, su uso del espa ol, del inglés y de formas lingüísticas híbridas, así como el reflejo literario de identidades mixtas. Se argumenta que, en esta situación inestable, la conjunción del espa ol y del inglés, con infinitas manifestaciones, canaliza la emergencia de facetas de identidad y de cauces de expresión aplaudidos por unos y combatidos por los más que, no obstante, son producto ineluctable del contacto de lenguas y de culturas.
Generation and Control of Turbulences in a Wind Tunnel  [PDF]
América Torres, Roberto Tapia, Antonio Ramos
Open Journal of Fluid Dynamics (OJFD) , 2016, DOI: 10.4236/ojfd.2016.64033
Abstract: In this paper, the generation and control of turbulences in a wind tunnel, for design, analysis and testing of test objects are proposed. Modifications to the wind tunnel are made in order to generate the turbulences in the test chamber. Specifically, for the turbulence analysis, the reflective symmetry concept is used in order to group the different turbulences cases generated in the wind tunnel test chamber. The Ansys software is used in order to model and analyze the wind tunnel. Specific platform or module known as Fluent is used for the analysis of the turbulence generated in the wind tunnel. To design proposal of a wind tunnel capable to generate and controlling turbulences is exposed in this paper. For this, the controller design and verification are performed by means of simulations. To obtain the control law structure, the inversion concept is used. The proposed control law is validated via a co-simulation implemented in the Simplorer module, with the aim of combining the power electronics part, controller and engine, with the proposed model wind tunnel.
Conservación de cepas vacunales de Leptospira en nitrógeno líquido
Borrero Maura,Reinier; Batista Santiesteban,Niurka; Blain Torres,Kirenia; Valdés Abreu,Yolanda;
Revista Cubana de Medicina Tropical , 2010,
Abstract: introduction: leptospira interrogans strains of cuban vaccine vax-spiral?are preserved in fletcher's semi-solid medium, which guarantees the preservation of the cell viability for a few months. objective: to evaluate the cryopreservation at liquid nitrogen of 6 different cryoprotectors as a long-term preservation method for leptospira interrogans strains used as antigens in vaccine vax-spiral? against leptospirosis. methods: viability was systematically evaluated according to the cell yield of the recently thawed strains in emjh medium. virulence stability was estimated in hamsters and antigenicity was evaluated by microscopic agglutination test using reference antisera from vaccinal serogroups. results: the use of 2.5% and 5% dimethyl sulfoxide, and 2.5% glycerol allowed quick recovery of the three strains without virulence or antigenicity depletion after 19 months of cryopreservation. conclusions: the results showed the feasibility of the cryopreservation at liquid nitrogen using suitable cryoprotectant as a preservation method for vaccinal strains of leptospira.
Page 1 /43904
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.