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Search Results: 1 - 10 of 386 matches for " Antje Rusch "
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Molecular Tools for the Detection of Nitrogen Cycling Archaea
Antje Rusch
Archaea , 2013, DOI: 10.1155/2013/676450
Abstract:
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Antje Rusch
Archaea , 2013, DOI: 10.1155/2013/676450
Abstract: Archaea are widespread in extreme and temperate environments, and cultured representatives cover a broad spectrum of metabolic capacities, which sets them up for potentially major roles in the biogeochemistry of their ecosystems. The detection, characterization, and quantification of archaeal functions in mixed communities require Archaea-specific primers or probes for the corresponding metabolic genes. Five pairs of degenerate primers were designed to target archaeal genes encoding key enzymes of nitrogen cycling: nitrite reductases NirA and NirB, nitrous oxide reductase (NosZ), nitrogenase reductase (NifH), and nitrate reductases NapA/NarG. Sensitivity towards their archaeal target gene, phylogenetic specificity, and gene specificity were evaluated in silico and in vitro. Owing to their moderate sensitivity/coverage, the novel nirB-targeted primers are suitable for pure culture studies only. The nirA-targeted primers showed sufficient sensitivity and phylogenetic specificity, but poor gene specificity. The primers designed for amplification of archaeal nosZ performed well in all 3 criteria; their discrimination against bacterial homologs appears to be weakened when Archaea are strongly outnumbered by bacteria in a mixed community. The novel nifH-targeted primers showed high sensitivity and gene specificity, but failed to discriminate against bacterial homologs. Despite limitations, 4 of the new primer pairs are suitable tools in several molecular methods applied in archaeal ecology. 1. Introduction Archaea have been detected in virtually all types of extreme and moderate environments. They play multiple ecological roles, colonizing certain newly emerging habitats [1, 2], interacting with animals such as corals [3, 4], sponges [5, 6], termites [7], or ruminants, forming part of microbe-microbe symbioses [8–10], and driving numerous processes in the biogeochemical C, N, S, and Fe cycles. In addition to relatively well-studied isolates of extremophilic or methanogenic Archaea, uncultured representatives have been detected by their 16S rRNA genes or by metabolic genes that classify their owners into the guilds of sulfate reducers, diazotrophs, ammonia oxidizers, or methanogens. Despite their widespread occurrence, a mere handful of nonmethanogenic Archaea has been isolated from moderate habitats [11–13]. While such isolates are indispensable for insight into archaeal ecophysiology, they have been recalcitrant to cultivation efforts, so that our current ecological research on mesophilic Archaea largely depends on cultivation-independent methods. Molecular
IRT models with relaxed assumptions in eRm: A manual-like instruction
REINHOLD HATZINGER,THOMAS RUSCH
Psychology Science Quarterly , 2009,
Abstract: Linear logistic models with relaxed assumptions (LLRA) as introduced by Fischer (1974) are a flexible tool for the measurement of change for dichotomous or polytomous responses. As opposed to the Rasch model, assumptions on dimensionality of items, their mutual dependencies and the distribution of the latent trait in the population of subjects are relaxed. Conditional maximum likelihood estimation allows for inference about treatment, covariate or trend effect parameters without taking the subjects' latent trait values into account. In this paper we will show how LLRAs based on the LLTM, LRSM and LPCM can be used to answer various questions about the measurement of change and how they can be fitted in R using the eRm package. A number of small didactic examples is provided that can easily be used as templates for real data sets. All datafiles used in this paper are available from http://eRm.R-Forge.R-project.org/
“Elude” – the making of an impossible game
Doris C. Rusch
E-Beratungsjournal , 2011,
Abstract: Right after the Games for Health Conference in 2009, Doris C. Rusch and Attila Ceranoglu decided to work on a game about depression: A game that actually intends to make the feelings of depression itself experientially tangible to players. They decided to use the internet and virtual reality to reach the digital natives easily, and to raise awareness of the difficulties and problems of depression amongst young people (and therefore become more willing to either seek help for themselves or others). But how to create such a game about depression? How to create a game that would be no fun? Rusch and Ceranoglu found the answers and now Elude“ is done!
Throwing the dice for the diagnosis of vaginal complaints?
Andreas Schwiertz, David Taras, Kerstin Rusch, Volker Rusch
Annals of Clinical Microbiology and Antimicrobials , 2006, DOI: 10.1186/1476-0711-5-4
Abstract: We evaluated the misjudgement rate of the aetiology of vaginal complaints. A total of 220 vaginal samples from women with a vaginal complaint were obtained and analysed for numbers of total lactobacilli, H2O2-producing lactobacilli, total aerobic cell counts and total anaerobic cell counts including bifidobacteria, Bacteroides spp., Prevotella spp. Additionally, the presence of Atopobium vaginae, Gardnerella vaginalis, Candida spp. and Trichomonas vaginalis was evaluated by DNA-hybridisation using the PCR and Affirm VPIII Microbial Identification Test, respectively.The participating physicians diagnosed Bacterial vaginosis (BV) as origin of discomfort in 80 cases, candidiasis in 109 cases and mixed infections in 8 cases. However, a present BV, defined as lack of H2O2-lactobacilli, presence of marker organisms, such as G. vaginalis, Bacteroides spp. or Atopobium vaginae, and an elevated pH were identified in only 45 cases of the women examined. Candida spp. were detected in 46 cases. Interestingly, an elevated pH corresponded solely to the presence of Atopobium vaginae, which was detected in 11 cases.Errors in the diagnosis of BV and candida vulvovaginitis (CV) were high. Interestingly, the cases of misjudgement of CV (77%) were more numerous than that of BV (61%). The use of Amsel criteria or microscopy did not reduce the number of misinterpretations. The study reveals that the misdiagnosis of vaginal complaints is rather high.The microbiology of the vagina is complex, containing 109 bacterial colony forming units per gram of secretions and potentially dozens of species. It is mainly dominated by members of the genus Lactobacillus, which are capable of H2O2-production and lactic acid, thereby maintaining the generally acidic vaginal pH. Age, phase of the menstrual cycle, sexual activity, contraceptive choice, pregnancy, presence of necrotic tissue or foreign bodies, and use of hygienic products or antibiotics can disrupt this ecosystem. A disturbed vaginal microbiot
Microfauna–Macrofauna Interaction in the Seafloor: Lessons from the Tubeworm
Antje Boetius
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0030102
Abstract:
Report on the conference on 'Men, Women, and Medicine: A New View of the Biology of Sex/Gender Differences and Aging' held in Berlin, 24–26th February 2006
Antje Kampf
Philosophy, Ethics, and Humanities in Medicine , 2006, DOI: 10.1186/1747-5341-1-11
Abstract: This three-day conference, the first world-wide symposium on the topic of gender-specific medicine, attracted a wide range of distinguished researchers and clinicians. The focus of the Conference was to report on the latest research on sex and/or gender difference and its impact on physiological function, treatment procedure and patient outcomes. The presentations ranged beyond the topic of reproduction – the most obvious field of sex difference – to encompass the entire human organism, with the scheduled 92 papers and 98 poster presentations covering diverse medical sub-specialities. Individual papers can be accessed as abstracts in the journal Gender & Medicine, 3, Supplement A, 2006, or on the website [1]. This report focuses on three issues that emerged during the Conference: gender mainstreaming, concepts of sex and gender difference, and men's health.The concept of gender mainstreaming was originally established by OSAGI in 1997, and was later also adopted by the WHO. Gender mainstreaming calls for equitable attention to male and female examples in clinical research, treatment, education, and indeed the whole health care system. It aims to ensure recognition and understanding of gender in social policy processes, and thereby counteract gender inequality [2]. Gender mainstreaming has been widely discussed, particularly by experts in health policy, and the literature is considerable. For a critical overview of the concept and implementation of gender mainstreaming, see, for example [3]. The purpose of gender-specific medicine is to achieve these goals – in addition to real benefits to patient care. Yet gender-specific medicine is still used as a euphemism for women's health alone, and the distinct concepts of sex and gender are often not accepted as scientific categories in medical research and practice, despite their substantial relevance [4]. Nevertheless, the array of newly-emerging research, and the range of the papers presented at this conference, indicates
Imaging in cochlear implant patients []
Aschendorff, Antje
GMS Current Topics in Otorhinolaryngology, Head and Neck Surgery , 2012, DOI: 10.3205/cto000080
Abstract: [english] Imaging procedures are a mainstream tool in the daily ENT workflow. Cochlear Implant patients are representing a special population with specific demands for imaging. There are different imaging techniques available for pre-operative evaluation, surgery and postoperative controls with different indications and consequences. High-resolution computed tomography and magnetic resonance imaging are mainly used in the evaluation process. New procedures, as digital volume tomography, are increasingly used intra- and postoperatively. Especially the intracochlear positioning in malformations of the inner ear, eventually added with radiological assisted navigation, can be considered a standard of modern cochlear implant surgery. In addition, digital volume tomography may serve as a quality control tool focusing on the evaluation of the intracochlear electrode position. The range of applications, indications and current results are illustrated.
conservation biogeography forum: Neil D. Burgess
Antje Ahrends
Frontiers of Biogeography , 2009,
Abstract:
Sprach(wissenschaft)liche Kulturen. Pl doyer für eine linguistische Partizipation an einem konstruktivistisch begründeten, kulturwissenschaftlichen Projekt transdisziplin rer Forschung am Beispiel der Interkulturellen Kommunikation
Hornscheidt, Antje
Linguistik Online , 2003,
Abstract: In my article I take a constructivist perspective as a starting point. Giving some introductory remarks on the role of language and the constitution of meaning within a constructivist framework, the article discusses the role and relevance of Critical Discourse Analysis and Cognitive Linguistics within this frame for cultural studies. It is argued that a transdisciplinary approach is most valuable for a research taking the importance of linguistic processes for cultural analysis into account. Following especially Fauconnier (1999) and Verschueren (1999), the importance of a pragmatic and cognitive-linguistic approach to language is underlined. To exemplify the consequences that a transdisciplinary approach could have, the article focuses on the way intercultural communication has been conceptualized within linguistics so far. The critical analysis of traditional research on and conceptualization of intercultural communication shows, how a constructivist perspective can widen the scope of linguistic research.
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