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Search Results: 1 - 10 of 209740 matches for " Antón Barreiro-Iglesias "
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Use of Fluorochrome-Labeled Inhibitors of Caspases to Detect Neuronal Apoptosis in the Whole-Mounted Lamprey Brain after Spinal Cord Injury
Antón Barreiro-Iglesias,Michael I. Shifman
Enzyme Research , 2012, DOI: 10.1155/2012/835731
Abstract: Apoptosis is a major feature in neural development and important in traumatic diseases. The presence of active caspases is a widely accepted marker of apoptosis. We report here the development of a method to study neuronal apoptotic death in whole-mounted brain preparations using fluorochrome-labeled inhibitors of caspases (FLICA). As a model we used axotomy-induced retrograde neuronal death in the CNS of larval sea lampreys. Once inside the cell, the FLICA reagents bind covalently to active caspases causing apoptotic cells to fluoresce, whereas nonapoptotic cells remain unstained. The fluorescent probe, the poly caspase inhibitor FAM-VAD-FMK, was applied to whole-mounted brain preparations of larval sea lampreys 2 weeks after a complete spinal cord (SC) transection. Specific labeling occurred only in identifiable spinal-projecting neurons of the brainstem previously shown to undergo apoptotic neuronal death at later times after SC transection. These neurons also exhibited intense labeling 2 weeks after a complete SC transection when a specific caspase-8 inhibitor (FAM-LETD-FMK) served as the probe. In this study we show that FLICA reagents can be used to detect specific activated caspases in identified neurons of the whole-mounted lamprey brain. Our results suggest that axotomy may cause neuronal apoptosis by activation of the extrinsic apoptotic pathway. 1. Introduction Studies in the basic neurosciences are heavily reliant upon rat and mouse models (for a review see [1]). Seventy-five percent of current research efforts are directed to rat mouse, and human brains, which represent 0.0001% of the nervous systems on the planet [1]. In recent decades, an increased number of studies have shown the usefulness of nonmammalian models for understanding developmental, pathological, and regenerative processes of the nervous system. Lampreys and fishes, for example, have proven to be valuable animal models for studying successful regeneration in the mature central nervous system (CNS) [2–5]. Lampreys occupy a key position close to the root of the vertebrate phylogenetic tree [6] and are thought to have existed largely unchanged for more than 500 million years, which makes them important animals from the standpoint of molecular evolution [7–10]. The unique evolutionary position of lampreys as early-evolved vertebrates, the sequencing of the sea lamprey (Petromyzon marinus L.) genome, and the adaptation and optimization of many established molecular biology and histochemistry techniques for use in this species have made it an emerging nonmammalian model organism
The Glutamatergic Neurons in the Spinal Cord of the Sea Lamprey: An In Situ Hybridization and Immunohistochemical Study
Blanca Fernández-López, Verona Villar-Cervi?o, Silvia M. Valle-Maroto, Antón Barreiro-Iglesias, Ramón Anadón, María Celina Rodicio
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0047898
Abstract: Glutamate is the main excitatory neurotransmitter involved in spinal cord circuits in vertebrates, but in most groups the distribution of glutamatergic spinal neurons is still unknown. Lampreys have been extensively used as a model to investigate the neuronal circuits underlying locomotion. Glutamatergic circuits have been characterized on the basis of the excitatory responses elicited in postsynaptic neurons. However, the presence of glutamatergic neurochemical markers in spinal neurons has not been investigated. In this study, we report for the first time the expression of a vesicular glutamate transporter (VGLUT) in the spinal cord of the sea lamprey. We also study the distribution of glutamate in perikarya and fibers. The largest glutamatergic neurons found were the dorsal cells and caudal giant cells. Two additional VGLUT-positive gray matter populations, one dorsomedial consisting of small cells and another one lateral consisting of small and large cells were observed. Some cerebrospinal fluid-contacting cells also expressed VGLUT. In the white matter, some edge cells and some cells associated with giant axons (Müller and Mauthner axons) and the dorsolateral funiculus expressed VGLUT. Large lateral cells and the cells associated with reticulospinal axons are in a key position to receive descending inputs involved in the control of locomotion. We also compared the distribution of glutamate immunoreactivity with that of γ-aminobutyric acid (GABA) and glycine. Colocalization of glutamate and GABA or glycine was observed in some small spinal cells. These results confirm the glutamatergic nature of various neuronal populations, and reveal new small-celled glutamatergic populations, predicting that some glutamatergic neurons would exert complex actions on postsynaptic neurons.
Early Development of the Cranial Nerves in a Primitive Vertebrate, the Sea Lamprey, Petromyzon Marinus L.
Petromyzon MarinusAntón Barreiro-Iglesias, María Pilar Gómez-López, Ramón Anadón and María Celina RodicioPetromyzon marinus
The Open Zoology Journal , 2008, DOI: 10.2174/1874336600801010037]
Abstract: The early development of the cranial nerves of the sea lamprey, Petromyzon marinus L., was studied in embryos and early prolarvae by immunocytochemical techniques with the marker for post-mitotic neurons acetylated α- tubulin. The trigeminal and facial nerves were first observed in embryos 9 days post fertilisation. The glossopharyngeal and vagal nerves appeared later, which indicates a rostrocaudal gradient in differentiation of branchiomeric nerves. The anterior and posterior lateral line, octaval and hypoglossal nerves also appeared in early developmental stages, but the ocular motor nerves were not observed in prolarvae. The present results indicate that, in comparison with cranial nerves and ganglia organisation reported in larval and adult lampreys, organisational changes occur in the cranial nerves between the prolarval and larval stages. One important change is the disappearance of the pharyngeal branch of the facial nerve, which was not previously reported to be present in larval and adult lampreys, whereas it had been observed in earlier developmental stages. Comparison of the present results with those from studies carried out in other vertebrate species, including the Japanese lamprey, suggests that the developmental pattern of the cranial nerves is conserved in agnathans and differs from that reported in other vertebrate groups. As maturation of lamprey eyes and extraocular muscles is completed at metamorphosis, there appears to be a correlation between the late development of eye-related cranial nerves in lampreys and the anatomical structures that they innervate.
Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes
Mónica Barriuso-Iglesias, Daniela Schluesener, Carlos Barreiro, Ansgar Poetsch, Juan F Martín
BMC Microbiology , 2008, DOI: 10.1186/1471-2180-8-225
Abstract: The cytoplasmatic and membrane proteome of Corynebacterium glutamicum ATCC 13032 at different pH conditions (6.0, 7.0 and 9.0) was analyzed by classical 2D-electrophoresis, and by anion exchange chromatography followed by SDS-PAGE (AIEC/SDS-PAGE). A few cytoplasmatic proteins showed differential expression at the three pH values with the classical 2D-technique including a hypothetical protein cg2797, L-2.3-butanediol dehydrogenase (ButA), and catalase (KatA). The AIEC/SDS-PAGE technique revealed several membrane proteins that respond to pH changes, including the succinate dehydrogenase complex (SdhABCD), F0F1-ATP synthase complex subunits b, α and δ (AtpF, AtpH and AtpA), the nitrate reductase II α subunit (NarG), and a hypothetical secreted/membrane protein cg0752. Induction of the F0F1-ATP synthase complex β subunit (AtpD) at pH 9.0 was evidenced by Western analysis. By contrast, L-2.3-butanediol dehydrogenase (ButA), an ATPase with chaperone activity, the ATP-binding subunit (ClpC) of an ATP-dependent protease complex, a 7 TMHs hypothetical protein cg0896, a conserved hypothetical protein cg1556, and the dihydrolipoamide acyltransferase SucB, were clearly up-regulated at pH 6.0.The observed protein changes explain the effect of the extracellular pH on the growth and physiology of C. glutamicum. Some of the proteins up-regulated at alkaline pH respond also to other stress factors suggesting that they serve to integrate the cell response to different stressing conditions.Since its discovery [1], Corynebacterium glutamicum ATCC 13032 is one of the most important organisms used industrially to produce not only various amino acids, but also vitamins, organic acids, proteins and diverse raw chemicals [2-4]. This strain is a facultative anaerobic Gram-positive soil bacterium with a high G+C content. Recently, the entire genome sequence of C. glutamicum ATCC 13032 has been determined in parallel in Japan [5] and in Germany [6]. Availability of the genome sequence allowed
Paediatric out-of-hospital resuscitation in an area with scattered population (Galicia-Spain)
Pilar Fernández, Luis Sánchez-Santos, Antonio Rodríguez-Nú?ez, José Iglesias-Vázquez, María Cegarra-García, Maria Barreiro-Díaz
BMC Emergency Medicine , 2007, DOI: 10.1186/1471-227x-7-3
Abstract: All children (aged from newborn to 16 years old) who suffered an out-of-hospital CRA in Galicia and were assisted by the Public Foundation Medical Emergencies of Galicia-061 staff, from June 2002 to February 2005, were included in the study. Data were prospectively recorded following the Utstein's style guidelines.Thirty-one cases were analyzed (3.4 CRA annual cases per 100.000 paediatric population). The arrest was respiratory in 16.1% and cardiac in 83.9% of cases. CRA occurred at home in 58.1% of instances. Time CRA to initiation of CPR was shorter than 10 minutes in 32.2% and longer than 20 minutes in 29.0% of cases. 22.6% of children received bystander CPR. The first recorded rhythm was asystole in 67.7% of cases. Bag-mask ventilation was used in 67.7% and in 83.8% oro-tracheal intubation was done. A peripheral venous access was achieved in 67.7% and intraosseous access was used in 16.1% of patients. 93.5% of children were treated with adrenaline. After initial CPR, sustained restoration of spontaneous circulation was achieved in 38.7% of cases. Six children (19.4%) survived until hospital discharge. Four of 5 children with respiratory arrest survived, whereas only 2 of 26 children with cardiac arrest survived until hospital discharge.Despite the handicap of a highly disseminated population, paediatric CRA characteristics and CPR results in Galicia are comparable to references from other communities. Programs to increase bystander CPR, equip laypeople with basic CPR skills and to update life support knowledge of health staff are needed to improve outcomes.Cardiorespiratory arrest (CRA) is a rare event during childhood that needs early and adequate treatment in order to achieve survival without neurological damage [1-6]. In addition to previous retrospective data, recent studies have increased our knowledge about characteristics and prognosis of CRA in children [1-7]. The Pediatric Utstein style has facilitated systematic recording and evaluation of data and per
Cost-efficiency assessment of Advanced Life Support (ALS) courses based on the comparison of advanced simulators with conventional manikins
José Iglesias-Vázquez, Antonio Rodríguez-Nú?ez, Mónica Penas-Penas, Luís Sánchez-Santos, Maria Cegarra-García, Maria Barreiro-Díaz
BMC Emergency Medicine , 2007, DOI: 10.1186/1471-227x-7-18
Abstract: Two hundred fifty primary care physicians and nurses were admitted to ten ALS courses (25 students per course). Students were distributed at random in two groups (125 each). Group A candidates were trained and tested with standard ALS manikins and Group B ones with new generation emergency and life support integrated simulator systems.In group A, 98 (78%) candidates passed the course, compared with 110 (88%) in group B (p < 0.01). The total cost of conventional courses was €7689 per course and the cost of the advanced simulator courses was €29034 per course (p < 0.001). Cost per passed student was €392 in group A and €1320 in group B (p < 0.001).Although ALS advanced simulator systems may slightly increase the rate of students who pass the course, the cost-effectiveness of ALS courses with standard manikins is clearly superior.Cardiopulmonary resuscitation (CPR) training needs specific courses built on experienced instructors, adequate lectures, skill-stations and the simulation of realistic scenarios. The availability of specifically designed manikins and the implementation of learning systems and course regulations, following Resuscitation Council's recommendations, have allowed for the effective training of a huge number of health care professionals [1-8].Simulation is a general term for an interactive educational strategy that has been shown to be highly useful in the qualification of professionals working in emergency conditions (like flight pilots and physicians) [9-12]. Similarly, new simulation systems, including sophisticated manikins and computers, represent an important step ahead in technology as well as in medical training possibilities.The theoretical advantages of "simulation system manikins" include: real-time records of the scenario (including responses or treatments applied by the student to the patient) allowing for a more accurate debriefing, more sophisticated manikin features (central and peripheral pulses, real cardiac and respiratory sounds,
Metástasis en partes blandas por un carcinoma micropapilar de vejiga: Metastatic disease
San Miguel Fraile,P.; Antón Badiola,I.; Ortiz Rey,J.A.; Fernández Fernández,G.; Iglesias Rodríguez,B.; Zungri Telo,E.;
Actas Urológicas Espa?olas , 2007, DOI: 10.4321/S0210-48062007000700017
Abstract: micropapillary transitional cell carcinoma is a rare (incidence of 0.7%) and highly aggressive variant of bladder carcinoma. morphologically, it is characterized by small tight clusters of neoplastic cell floating in clear spaces resembling lymphatic channels. its usual presentation is like a high grade and stage carcinoma and most often is associated with a variable component of conventional carcinoma or other variants. the usual sites of bladder cancer metastases are the lymph nodes, lungs, bone and liver. soft tissues metastases from transitional cell carcinoma of the bladder occur infrequently. we report the cases of a 77-year-old man presenting with an abdominal soft tissue mass a six years after local excision of a micropapillary bladder carcinoma.
Expresión de CDX2 en lesiones de vejiga urinaria y uretra
Ortiz-Rey,J.A.; Antón Badiola,I.; San Miguel Fraile,P.; álvarez álvarez,C.; Iglesias Rodríguez,B.; Zungri-Telo,E.;
Actas Urológicas Espa?olas , 2004, DOI: 10.4321/S0210-48062004000200005
Abstract: background: cdx1 and cdx2 are transcription factors involved in the development and maintenance of the intestinal epithelial cell. expression of cdx2 has been reported in normal and metaplastic intestinal epithelium, and in those adenocarcinomas with that cellular origin. we have analized the expression of this marker in reactive and tumoral lesions arising in urinary bladder, urethra and urachus. method: cdx2 was investigated through immunohistochemistry on paraffin-embedded tissue, using the labelled streptavidin-biotin method (lsab2, dako) with a monoclonal antibody (cdx2-88, biogenex). results: expression of cdx2 was observed in intestinal-type cistitis glandularis, intestinal metaplasia of urinary bladder, bladder adenocarcinoma, mucinous urothelial-type carcinoma of prostatic urethra and urachal mucinous carcinoma. cdx2 was not detected in normal urothelium and prostatic glandular epithelium, von brunn nests, typical-type cistitis glandularis, glandular adenosis and transitional carcinoma. conclusions: lesions, both benign and malignant, with enteric-cell morphological features show positivity for cdx2. expression of this marker is not organ-specific but is just related to a cellular phenotype. reactivity for cdx2 in an adenocarcinoma can be consistent with an origin in urinary tract or urachus.
Expresión de CD10 y del marcador de carcinoma renal en el carcinoma renal de células claras: Análisis en matrices tisulares
Ortiz-Rey,J.A.; Gómez de María,C.; Peláez Boismorand,E.; Fernández Costas,A.; Barbosa Barreiro,M.J.; Antón Badiola,I.;
Actas Urológicas Espa?olas , 2006, DOI: 10.4321/S0210-48062006000300005
Abstract: objectives: cd10 and renal cell carcinoma (rcc) marker antibodies react against proteins of the epithelium of the renal proximal tubule, being expressed by renal cell carcinomas. the frequence and pattern of expression of both markers are analysed in a series of clear cell renal cell carcinomas. method: two tissue arrays were used, which were composed of cylinders obtained with a 16g needle from 40 paraffin blocks that corresponded to clear cell renal cell carcinomas. the labeled streptavidin-biotin technique was performed (lsab2, dako) using cd10 and rcc monoclonal antibodies (novocastra), testing different antigen retrieval methods for rcc. immunoreactivity was evaluated as + (isolated cells or focal staining); ++ (moderate) and +++ (extense). results: thirty cases (75%) were positive for cd10: 12 +; 5 ++ and 13 +++. the best antigen retrieval method for rcc was a double enzyme digestion (trypsin + protease). twenty cases (50%) were positive for rcc: 7 +; 5 ++ and 8 +++. four cases out of the 20 immunoreactive for rcc were negative for cd10. the 16 remaining cases also expressed cd10. conclusions: cd10 and rcc are often expressed by clear cell renal cell carcinomas, and they may be useful markers to suggest a renal origin of carcinomas. rcc is less sensitive than cd10. staining for both of them is usually focal, and thus sensitivity of these techniques decreases when small samples are investigated, such as tissue arrays. the antigen retrieval method is essential for rcc immunohistochemical detection, obtaining the best results with the use of proteolytic enzymes.
Amistades, sentimientos y drogas: Tras cesar el abuso
Millán Barreiro,Ma Pilar;
Index de Enfermería , 2011, DOI: 10.4321/S1132-12962011000300013
Abstract: drug addiction is a great problem at current society. we must not lose the individual perspective to take in consideration this social and public health problem, since thanks to the statements of them who live with drug addiction, we will have the possibility of being able design prevention programmes and strategies. it does a phenomenological qualitative study through the interview in depth to an informant, who voluntarily provided to tell us his experience, how he started, what he felt and how he left it.
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