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Search Results: 1 - 10 of 1271 matches for " Annette Moter "
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Co-Localized or Randomly Distributed? Pair Cross Correlation of In Vivo Grown Subgingival Biofilm Bacteria Quantified by Digital Image Analysis
Claudia Schillinger, Annett Petrich, Renate Lux, Birgit Riep, Judith Kikhney, Anton Friedmann, Lawrence E. Wolinsky, Ulf B. G?bel, Holger Daims, Annette Moter
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0037583
Abstract: The polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. A number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. To examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analysis tool of the software daime (digital image analysis in microbial ecology). This modification enabled the quantitative analysis of microbial co-localization in images of subgingival biofilm species, where the biomass was confined to fractions of the whole-image area, a situation common for medical samples. Selected representatives of the disease-associated red and orange complexes that were previously suggested to interact with each other in vitro (Tannerella forsythia with Fusobacterium nucleatum and Porphyromonas gingivalis with Prevotella intermedia) were chosen for analysis and labeled with specific fluorescent probes via fluorescence in situ hybridization. Pair cross-correlation analysis of in vivo grown biofilms revealed tight clustering of F. nucleatum/periodonticum and T. forsythia at short distances (up to 6 μm) with a pronounced peak at 1.5 μm. While these results confirmed previous in vitro observations for F. nucleatum and T. forsythia, random spatial distribution was detected between P. gingivalis and P. intermedia in the in vivo samples. In conclusion, we successfully employed spatial arrangement analysis on the single cell level in clinically relevant medical samples and demonstrated the utility of this approach for the in vivo validation of in vitro observations by analyzing statistically relevant numbers of different patients. More importantly, the culture-independent nature of this approach enables similar quantitative analyses for “as-yet-uncultured” phylotypes which cannot be characterized in vitro.
Filifactor alocis - involvement in periodontal biofilms
Sebastian Schlafer, Birgit Riep, Ann L Griffen, Annett Petrich, Julia Hübner, Moritz Berning, Anton Friedmann, Ulf B G?bel, Annette Moter
BMC Microbiology , 2010, DOI: 10.1186/1471-2180-10-66
Abstract: A species-specific oligonucleotide probe, FIAL, was designed and evaluated. A total of 490 subgingival plaque samples were submitted to PCR and subsequent dot blot hybridization to compare the prevalence of F. alocis in patients suffering from generalized aggressive periodontitis (GAP), chronic periodontitis (CP), and control subjects resistant to periodontitis. Moreover, a specially designed carrier system was used to collect in vivo grown subgingival biofilms from GAP patients. Subsequent topographic analysis was performed using fluorescence in situ hybridization.While the majority of patients suffering from GAP or CP harboured F. alocis, it was rarely detected in the control group. In the examined carrier-borne biofilms the organism predominantly colonized apical parts of the pocket in close proximity to the soft tissues and was involved in numerous structures that constitute characteristic architectural features of subgingival periodontal biofilms.F. alocis is likely to make a relevant contribution to the pathogenetic structure of biofilms accounting for periodontal inflammation and can be considered an excellent marker organism for periodontal disease.Periodontitis is a chronic inflammatory bacterial infection leading to destruction of periodontal ligaments and supporting bone of the tooth. Its aetiology has been a field of intensive research in the past decades. As periodontal pockets accommodate a multitude of bacterial phylotypes, it is difficult to differentiate between mere commensals and true pathogens. During the 1970's, 80's and early 90's, research focused mainly on a number of culturable bacteria like Porphyromonas gingivalis, Prevotella intermedia, Aggregatibacter (Actinobacillus)actinomycetemcomitans, Tannerella forsythia and Treponema denticola that proved to be associated with the disease [1]. Studies have determined their relative prevalences, interactions and virulence factors [2-7]. By the end of the 1980's, the development of novel, culture-in
Molecular Characterization of Borrelia persica, the Agent of Tick Borne Relapsing Fever in Israel and the Palestinian Authority
Gracia Safdie,Iba Y. Farrah,Reem Yahia,Esther Marva,Amos Wilamowski,Samer S. Sawalha,Naama Wald,Judith Schmiedel,Annette Moter,Ulf B. G?bel,Herve Bercovier,Ziad Abdeen,Marc V. Assous,Yolanta Fishman
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0014105
Abstract: The identification of the Tick Borne Relapsing Fever (TBRF) agent in Israel and the Palestinian Authority relies on the morphology and the association of Borrelia persica with its vector Ornithodoros tholozani. Molecular based data on B. persica are very scarce as the organism is still non-cultivable. In this study, we were able to sequence three complete 16S rRNA genes, 12 partial flaB genes, 18 partial glpQ genes, 16 rrs-ileT intergenic spacers (IGS) from nine ticks and ten human blood samples originating from the West Bank and Israel. In one sample we sequenced 7231 contiguous base pairs that covered completely the region from the 5′end of the 16S rRNA gene to the 5′end of the 23S rRNA gene comprising the whole 16S rRNA (rrs), and the following genes: Ala tRNA (alaT), Ile tRNA (ileT), adenylosuccinate lyase (purB), adenylosuccinate synthetase (purA), methylpurine-DNA glycosylase (mag), hypoxanthine-guanine phosphoribosyltransferase (hpt), an hydrolase (HAD superfamily) and a 135 bp 5′ fragment of the 23S rRNA (rrlA) genes. Phylogenic sequence analysis defined all the Borrelia isolates from O. tholozani and from human TBRF cases in Israel and the West Bank as B. persica that clustered between the African and the New World TBRF species. Gene organization of the intergenic spacer between the 16S rRNA and the 23S rRNA was similar to that of other TBRF Borrelia species and different from the Lyme disease Borrelia species. Variants of B. persica were found among the different genes of the different isolates even in the same sampling area.
A New Biological Strategy for Drug Delivery: Eucaryotic Cell-Derived Nanovesicles  [PDF]
Irène Tatischeff, Annette Alfsen
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2011, DOI: 10.4236/jbnb.2011.225060
Abstract: An efficient drug delivery is the prerequisite of the successful chemotherapeutic treatments of many human diseases. Despite a great number of approaches, the improvement of drug cell internalization remains an actual research challenge. We propose a new biological delivery system based on the extracellular vesicles released by a non-pathological eukaryotic microorganism, Dictyostelium discoideum. After a summary of the main characteristics of these extracellular vesicles, including of their lipid bilayer that appears as a good candidate for initiating membrane fusion, followed by delivery of their encapsulated drug, the capacity of these vesicles to convey drugs into human cells was demonstrated in vitro on two tumor cell lines, resistant leukaemia K562r and cervix carcinoma HeLa cells. A comparison with other extracellular vesicles, like exosomes or bacteria-derived particles, stresses the unique properties of Dictyostelium extracellular nanovesicles for drug delivery.
The Lipid Bilayer of Biological Vesicles: A Liquid-Crystalline Material as Nanovehicles of Information  [PDF]
Annette Alfsen, Irène Tatischeff
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2014, DOI: 10.4236/jbnb.2014.52013
Abstract:

The biological intracellular vesicles, formed from the cell membrane or from different cell organelles, play a main role in the intracellular transport, transfer and exchange of molecules and information. Extracellular vesicles are also detected in organisms belonging to any of the three main branches of evolution, Archaea, Bacteria and Eukarya. There is an increasing consensus that these vesicles are important mediators of intercellular communication. All the intracellular and extracellular vesicles present a characteristic lipid composition and organization that governs their formation, targeting and function. This paper gives an overview of the lipid chemical and physical structure, strongly related to their biological function. The properties and role of the different types of lipids from membranes and vesicles are described. Then, their physical structure is shown as self-associated in a bilayer and organized as a lyotropic liquid crystal. The present paper underlies the structural similarity between these biological vesicles and a new synthetic material, theliquid crystalline fullerodendrimers” obtained from the biological model. It is composed of a basket of carbon associated with a liquid crystalline material and has been shown to exhibit highly efficient properties of information transfer. Our observation stresses the essential role of the liquid crystalline structure of lipids in their function as biological nanovehicles of information. The comparison with the synthetic material contributes to a better understanding of the role of lipids for cell communication in living organisms.

A desigualdade invisível: o papel da classe social na cria??o dos filhos em famílias negras e brancas
Lareau, Annette;
Educa??o em Revista , 2007, DOI: 10.1590/S0102-46982007000200002
Abstract: although family life has an important impact on children's chances in life, the mechanisms through which parents transmit advantages are imperfectly understood. an ethnographic data set of white and black children around 10 years old shows the effects of social class on interactions at home. middle-class parents engage in concerted cultivation by attempting to foster children's talents through organized leisure activities and extensive reasoning. working-class and poor parents engage in the accomplishment of natural growth, providing the conditions under which children can grow, but leaving leisure activities to children themselves. these parents also use commands rather than reasoning. middle-class children, both white and black, gain an emerging sense of entitlement from their family life. race had much less impact than social class. also, differences in a cultural logic of raising children gave parents and their children differential resources to draw on in their interactions with professionals from dominant classes and other adults outside home. middle-class children gained individually insignificant but cumulatively important advantages. working-class and poor children did not display the same sense of entitlement or advantages. some areas of family life appeared immune from the effects of social class, however.
Review: "Seisoene" By Engela van Rooyen (2005)
Annette Jordaan
Tydskrif vir letterkunde , 2006,
Abstract: Kaapstad: Tafelberg 378 pp ISBN 0-62404-261-8 Tydskrif vir Letterkunde 43(1) 2006: 194-196
Review: "Bestemmings:, By Marita van der Vyver (2005)
Annette Jordaan
Tydskrif vir letterkunde , 2006,
Abstract: Kaapstad: Tafelberg 216 pp ISBN 0-624-04327-4 Tydskrif vir Letterkunde 43(2) 2006: 246-247
Virtue versus Piety
Pitschmann, Annette
Ars Disputandi : the Online Journal for Philosophy of Religion , 2012,
Abstract:
Die neue Hochschulbibliothek für Gesundheitswissenschaften der Hochschule für Gesundheit (hsg) Bochum [The new Academic Health Sciences, Nursing and Therapeutics Library of the Hochschule für Gesundheit (hsg) Bochum]
Kustos, Annette
GMS Medizin-Bibliothek-Information , 2012, DOI: 10.3205/mbi000257
Abstract: [english] October 2009 the first state-funded University of Applied Sciences referring the fields public health, therapeutics and occupational medicine, the “Hochschule für Gesundheit hsg”, was founded in the City of , North Rhine-Westfalia. The study of health care/nursing, physical therapy, ergotherapy, logopedics and midwifery puts emphasis on an academic training for improving healthcare and therapeutics in German hospitals, medical offices and home care. Therefore a library had to be established which offers both practical text and specialist books and electronic resources and research material such as special research databases and journals. Temporarily located in only three rooms the library started setting up these electronic services within an e-learning environment and providing students and teachers with the important print material. The library also developed an integrated concept of functions and services, a “library development plan”, to ensure that a strategic process for elaborating the services is initialized in addition to the daily performance of improvisation skills. As to the establishment of a high level subject and usage related collection, media concept etc. the librarian in this new combined field of health and therapeutics finds himself in an unknown territory. There are no completely fitting statistics or models of other libraries to lean on. But by evaluating the usage of the offered media and services and observing developments in health sciences and its information market within a testing period the “canon” will be identified.In 2014 the library will be one of the main attractions on the “Health Campus Bochum” where the university and cooperating health research institutes, health administration and health care companies will be situated. Therefore the new library area in the second floor of a new building has to be planned involving the topics library workflows, circulation by RFID, shelving, systematic order, learning areas with user seats, accessibility for handicapped people etc. [german] Im Oktober 2009 wurde die Hochschule für Gesundheit – hsg – University of Applied Sciences – als erste staatliche Hochschule für die Themenfelder ffentliche Gesundheitsversorgung, praktische Medizin, Pflege, Therapie in Bochum gegründet. Ein wissenschaftliches Studium der F cher Pflege, Physiotherapie, Ergotherapie, Logop die und Hebammenkunde soll auf der Grundlage des Prinzips der evidenzbasierten Medizin Gesundheitsversorgung und therapeutische Behandlungen in Krankenh usern, Praxen und in der h uslichen Betreuung professionalisiere
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