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Mild hydrochloric acid hydrolysis of i-carrageenan
from Eucheuma spinosum yielded two
oligosaccharides of sulfated tetrasaccharide structure. These were
characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear
Magnetic Resonance (NMR) and Electrospray Ionization Mass Spectrometry (ESIMS).
Both oligosaccharides have structure of b-D-galactopyranose(Galp)4S-(1→4)-α-D-AnGalp2S-(1→3)-b-D-galactopyranose Galp)4S-(1→4)-α-D-AnGalp2S-(1→3). Application of the resulting oligosaccharides on protein
delivery system in terms of encapsulation efficiency was performed.