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Search Results: 1 - 10 of 13715 matches for " Anna Grzanka "
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Actin filament reorganization in HL-60 leukemia cell line after treatment with G-CSF and GM-CSF.
Alina Grzanka,Magdalena Izdebska,Anna Litwiniec,Dariusz Grzanka
Folia Histochemica et Cytobiologica , 2007, DOI: 10.5603/4523
Abstract: Currently, information regarding the influence of growth factors on the cytoskeleton, including G-CSF and GMCSF, remains limited. In the present study we show alterations in F-actin distribution and cell cycle progression in HL-60 promyelocytic leukemia cells, resulting from treatment with these cytokines in vitro. We found that both agents caused F-actin reorganization. Although multiple potential effects of various growth factors have been described previously, in our experimental conditions, we observed some rather subtle differences between the effects of G-CSF and GM-CSF on studied cells. The presence of these cytokines in the cell environment caused not only increased F-actin labeling in the cytoplasm, but also a weaker intensity of peripheral ring staining in comparison with control cells. In spite of the fact that HL60 cells exposed to G-CSF and GM-CSF contained different F-actin structures such as aggregates and F-actin network, the rate of actin polymerization was not significantly enhanced. Moreover, alterations were mainly related to considerable changes in the relative proportion of these different structures, what might be reflected by specific features of the differentiation process, with regard to the kind of stimulating factor used. Thus, reorganization of F-actin and other results obtained in our experimental conditions, might reflect unique characteristics of the differentiation process in HL-60 cells, involving low apoptosis frequency, the G1 to S phase transition in the cell cycle, as well as possible alternative ways of the cell death.
Rho proteins the key regulators of cytoskeleton in the progression of mitosis and cytokinesis
Anna Klimaszewska,Anna Stenzel,Jakub Marcin Nowak,Alina Grzanka
Post?py Higieny i Medycyny Do?wiadczalnej , 2011,
Abstract: The Rho proteins are members of the Ras superfamily of small GTPases. They are thought to be crucial regulators of multiple signal transduction pathways that influence a wide range of cellular functions, including migration, membrane trafficking, adhesion, polarity and cell shape changes. Thanks to their ability to control the assembly and organization of the actin and microtubule cytoskeletons, Rho GTPases are known to regulate mitosis and cytokinesis progression. These proteins are required for formation and rigidity of the cortex during mitotic cell rounding, mitotic spindle formation and attachment of the spindle microtubules to the kinetochore. In addition, during cytokinesis, they are involved in promoting division plane determination, contractile ring and cleavage furrow formation and abscission. They are also known as regulators of cell cycle progression at the G1/S and G2/M transition. Thus, the signal transduction pathways in which Rho proteins participate, appear to connect dynamics of actin and microtubule cytoskeletons to cell cycle progression. We review the current state of knowledge concerning the molecular mechanisms by which Rho GTPase signaling regulates remodeling of actin and microtubule cytoskeletons in order to control cell division progression.
Systemic Thrombolysis for Intraoperative, High Risk Pulmonary Embolism, Clinical Case  [PDF]
Anna Grzanka, Pilar Reboto, Alfredo Plaza, Patricia Gaite
Open Access Library Journal (OALib Journal) , 2019, DOI: 10.4236/oalib.1105411
A 68-year-old woman with intercondylar fracture of the femur suffers massive (high risk) pulmonary embolism (PE) during surgery and is suc-cessfully treated with systemic thrombolysis. After limb exsanguinations with Esmarch bandage, the patient presents with sudden oxygen desaturation (99% to 80%) with subsequent dyspnea and hypotension. She is intubated and requires continuous adrenalin perfusion while the surgery is finished and until she can be transferred to the reanimation ward. We decide to perform transthoracic echocardiogram (her general condition impedes transfer to tomography) which confirms right ventricular overload and pulmonary hypertension. These findings justify systemic thrombolysis, which is performed with good results. Surgery, and in particular orthopedic surgery, increase the risk of PE. When considering high risk PE, guidelines recommend primary reperfusion strategy through systemic thrombolysis (which can be contraindicated in surgery patients) or catheter-assisted thrombus removal (less widely available). Lately, surgical pulmonary embolectomy is being discussed as a treatment option for patients with contraindication to thrombolysis, but this practice is still uncommon.
Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells. Does the response involve senescence? The possible role of vimentin
Anna Litwiniec, Lidia Gackowska, Anna Helmin-Basa, Agnieszka ?ury?, Alina Grzanka
Cancer Cell International , 2013, DOI: 10.1186/1475-2867-13-9
Abstract: After treatment with etoposide, selected biochemical and morphological parameters were examined, including: the activity of senescence-associated Ss-galactosidase, SAHF formation, cell cycle progression, the induction of p21Cip1/Waf1/Sdi1 and cyclin D1, DNA strand breaks, the disruption of cell membrane asymmetry/integrity and ultrastructural alterations. Vimentin and G-actin cytoskeleton was evaluated both cytometrically and microscopically.Etoposide induced a senescence-like phenotype in the population of A549 cells. Morphological alterations were nevertheless not directly coupled with other senescence markers including a stable cell cycle arrest, SAHF formation or p21Cip1/Waf1/Sdi1 induction. Instead, a polyploid, TUNEL-positive fraction of cells visibly grew in number. Also upregulation of cyclin D1 was observed. Here we present preliminary evidence, based on microscopic analyses, that suggest a possible role of vimentin in nuclear alterations accompanying polyploidization-depolyploidization events following genotoxic insults.
Modelling beam transport and biological effectiveness to develop treatment planning for ion beam radiotherapy
Leszek Grzanka
Physics , 2014,
Abstract: Radiation therapy with carbon ions is a novel technique of cancer radiotherapy, applicable in particular to treating radioresistant tumours at difficult localisations. Therapy planning, where the medical physicist, following the medical prescription, finds the optimum distribution of cancer cells to be inactivated by their irradiation over the tumour volume, is a basic procedure of cancer radiotherapy. The main difficulty encountered in therapy planning for ion radiotherapy is to correctly account for the enhanced radiobiological effectiveness of ions in the Spread Out Bragg Peak (SOBP) region over the tumour volume. In this case, unlike in conventional radiotherapy with photon beams, achieving a uniform dose distribution over the tumour volume does not imply achieving uniform cancer cell inactivation. In this thesis, an algorithm of the basic element (kernel) of a treatment planning system (TPS) for carbon ion therapy is developed. The algorithm consists of a radiobiological part which suitably corrects for the enhanced biological effect of ion irradiation of cancer cells, and of a physical beam transport model. In the radiobiological component, Katz's track structure model of cellular survival is applied, after validating its physical assumptions and improving some aspects of this model. The Katz model offers fast and accurate predictions of cell survival in mixed fields of the primary carbon ions and of their secondary fragments. The physical beam model was based on available tabularized data, prepared earlier by Monte Carlo simulations. Both components of the developed TPS kernel are combined within an optimization tool, allowing the entrance energy-fluence spectra of the carbon ion beam to be selected in order to achieve a pre-assumed uniform (flat) depth-survival profile over the SOBP region, assuring uniform cancer cell inactivation over the tumour depth.
Organelle Cross-Talk in Apoptotic and Survival Pathways
Lina Ghibelli,Alina Grzanka
International Journal of Cell Biology , 2012, DOI: 10.1155/2012/968586
Organelle Cross-Talk in Apoptotic and Survival Pathways
Lina Ghibelli,Alina Grzanka
International Journal of Cell Biology , 2012, DOI: 10.1155/2012/968586
Vowel recognition of patients after total laryngectomy using Mel Frequency Cepstral Coefficients and mouth contour
Pietruch Rafal W.,Grzanka Antoni D.
Journal of Automatic Control , 2010, DOI: 10.2298/jac1001033p
Abstract: The paper addresses a problem of isolated vowels recognition in patients following total laryngectomy. The visual and acoustic speech modalities were separately incorporated in the machine learning algorithms. The authors used the Mel Frequency Cepstral Coefficients as acoustic descriptors of a speech signal. A lip contour was extracted from a video signal of the speaking faces using OpenCV software library. In a vowels recognition procedure the three types of classifiers were used for comparison purposes: Artificial Neural Networks, Support Vector Machines and Naive Bayes. The highest recognition rate was evaluated using Support Vector Machines. For a group of the laryngectomees having a different quality of speech the authors achieved 75% for acoustic and 40% for visual recognition performances. The authors obtained higher recognition rate than in a previous research where 10 cross-sectional areas of a vocal tract were estimated. Using presented image processing algorithm the visual features can be extracted automatically from a video signal.
TGF-β1 in bronchoalveolar lavage fluid in diffuse parenchymal lung diseases and high-resolution computed tomography score
Artur Szlubowski,Jerzy Soja,Piotr Grzanka,Romana Tomaszewska
Polish Archives of Internal Medicine , 2010,
Abstract: INTRODUCTION: In the pathogenesis of diffuse parenchymal lung diseases (DPLDs), growth factors, including transforming growth factor β1 (TGF-β1), are responsible for cell proliferation, apoptosis, chemotaxis, and angiogenesis, and also for the production and secretion of some components of the extracellular matrix. OBJECTIVES: The aim of the study was to evaluate correlations in DPLDs between TGF-β1 levels in bronchoalveolar lavage (BAL) fluid and high-resolution computed tomography (HRCT) score. PATIENTS AND METHODS: The study was performed in 31 DPLD patients in whom a selection of lung segments with high and low intensity of abnormalities was estimated by HRCT score. All patients underwent BAL with TGF-β1 measured by an enzyme immunoassay in BAL fluid and video-assisted thoracic surgery lung biopsy from both selected segments. RESULTS: All 31 patients were diagnosed, and based on histopathology, they were classified into 2 groups: idiopathic interstitial pneumonia (usual interstitial pneumonia – 12, nonspecific interstitialpneumonia – 2, cryptogenic organizing pneumonia – 2, and desquamative interstitial pneumonia – 1) and granulomatous disease (sarcoidosis – 7, extrinsic allergic alveolitis – 5, and histiocytosis X – 2). The final analysis was performed in 28 patients who showed nonhomogenous distribution on HRCT. TGF-β1 levels in BAL fluid were significantly higher in the areas with high intensity of abnormalities assessed by HRCT score (P = 0.018, analysis of variance). These levels were not different between the groups, but a trend towards higher levels in idiopathic interstitial pneumonia was observed. CONCLUSIONS: The results confirm that TGF-β1 may be a good but not specific marker of fibrosis in DPLDs. A significant positive correlation between TGF-β1 levels in BAL fluid and the HRCT score was observed.
Leczenie miejscowe tr dziku
Waldemar Placek,Krystyna Romańska-Gocka,Aleksandra Grzanka
Przegl?d Dermatologiczny , 2011,
Abstract: Acne vulgaris is a common disease of adolescence that occurs in approximately80% of the population aged 11-30 years. The disease greatlyreduces well-being and self-esteem. In the pathogenesis of acne vulgarismany factors take part: genetic, hormonal, sebaceous gland hyperplasiawith seborrhoea, changes in the composition of sebum, comedonesformation, Propionibacterium acnes colonization and inflammation.In most cases, the disease does not require systemic treatment, and theavailable causal therapy and topical acne skin care are sufficient toimprove skin condition. In acne vulgaris, local systematic treatmentadjusted to the form of the disease, as monotherapy or combinationtherapy, is required. Keratolytic and anti-comedone drugs are salicylicacid, retinoids and benzoyl peroxide. Some antibiotics, such as clindamycin, erythromycin and erythromycini cyclocarbonas, as well as benzoylperoxide, have anti-bacterial properties. Antibiotics such asmacrolides and tetracyclines, benzoyl peroxide, retinoids, azelaic acid,and vitamin B3 have anti-inflammatory properties. Only azelaic acidhas a weak anti-seborrhoeic effect. 17β-oestradiol and zinc ions havethe action of anti-androgens by competitive action on the receptors.The only substances that theoretically affect all elements of the aetiopathogenesisare triethyl citrate, linolan acetate and azelaic acid. Thearticle describes the mechanisms of action of topical preparations andthe main indications for their use according to the form of the acne.
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