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Search Results: 1 - 10 of 7305 matches for " Angelo Michele Carella "
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Angelo Michele Carella
Mediterranean Journal of Hematology and Infectious Diseases , 2012, DOI: 10.4084/mjhid.2012.
Abstract: Hodgkin lymphoma is one of the most curable human tumors. Despite this, about 30% of these patients relapsed or are primary refractory to the first line treatment. Autografting is generally considered the standard of care for these patients. Alternative salvage strategies have been evaluated such as high dose sequential and tandem autografting strategies. In younger patients, refractory or early relapsed after autografting, allogeneic stem cell transplantation has been employed but this approach has been followed by significant concerns since the treatment related mortality often exceeded 40-50%, and relapses were not uncommon. It is clear that patient selection remains an issue in all allografting reports. At the end, new drugs and novel treatment strategies, that are based on our understanding of the disease biology and signaling pathways, are needed to improve treatment outcome for these patients. The two leading compounds Brentuximab Vedotin and Panobinostat, are currently under evaluation in several clinical trials.
High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas
Paola Parrella,Antonella la Torre,Massimiliano Copetti,Vanna M. Valori,Raffaela Barbano,Angelo Notarangelo,Michele Bisceglia,Antonietta Pia Gallo,Teresa Balsamo,Maria Luana Poeta,Massimo Carella,Domenico Catapano,Salvatore Parisi,Bruno Dallapiccola,Evaristo Maiello,Vincenzo D'Angelo,Vito Michele Fazio
Journal of Biomedicine and Biotechnology , 2009, DOI: 10.1155/2009/531692
Abstract: Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (=.000009 Mann Whitney Test) and frequencies (=.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93%; CI95%: 84%–100%) as compared with MSP (64%; 95%CI: 46%–82%). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
Promoter methylation correlates with reduced NDRG2 expression in advanced colon tumour
Ada Piepoli, Rosa Cotugno, Giuseppe Merla, Annamaria Gentile, Bartolomeo Augello, Michele Quitadamo, Antonio Merla, Anna Panza, Massimo Carella, Rosalia Maglietta, Annarita D'Addabbo, Nicola Ancona, Saverio Fusilli, Francesco Perri, Angelo Andriulli
BMC Medical Genomics , 2009, DOI: 10.1186/1755-8794-2-11
Abstract: The validation assay was performed in a different set of 8 patients with colorectal cancer (CRC) by means quantitative reverse-transcriptase polymerase chain reaction analysis. The differential RNA expression profiles of three CRC cell lines before and after 5-aza-2'-deoxycytidine treatment were compared to identify the hypermethylated genes. The DNA methylation status of these genes was evaluated by means of bisulphite genomic sequencing and methylation-specific polymerase chain reaction (MSP) in the 3 cell lines and in tumour tissues from 30 patients with CRC.Data from our previous genome search have received confirmation in the new set of 8 patients with CRC. In this validation set six genes showed a high induction after drug treatment in at least two of three CRC cell lines. Among them, the N-myc downstream-regulated gene 2 (NDRG2) promoter was found methylated in all CRC cell lines. NDRG2 hypermethylation was also detected in 8 out of 30 (27%) primary CRC tissues and was significantly associated with advanced AJCC stage IV. Normal colon tissues were not methylated.The findings highlight the usefulness of combining gene expression patterns and epigenetic data to identify tumour biomarkers, and suggest that NDRG2 silencing might bear influence on tumour invasiveness, being associated with a more advanced stage.Colorectal cancer (CRC) is the third most common cancer in men and women, accounting for 11% of all cancer-related deaths. The majority of cases are diagnosed in advanced stages when a curative treatment is less likely to occur and chemotherapy is the only option [1]. The identification of the molecular, genetic, and epigenetic changes underlying the adenoma-carcinoma sequence [2] leading to CRC has been the focus of many researches [3]. It is now widely accepted that sporadic CRC frequently arises from preneoplastic lesions through the activation of proto-oncogenes, such as K-ras, and the inactivation of tumor suppressor genes (TSG), such as APC, p53, DCC,
Differences in Gene Expression and Cytokine Release Profiles Highlight the Heterogeneity of Distinct Subsets of Adipose Tissue-Derived Stem Cells in the Subcutaneous and Visceral Adipose Tissue in Humans
Sebastio Perrini, Romina Ficarella, Ernesto Picardi, Angelo Cignarelli, Maria Barbaro, Pasquale Nigro, Alessandro Peschechera, Orazio Palumbo, Massimo Carella, Michele De Fazio, Annalisa Natalicchio, Luigi Laviola, Graziano Pesole, Francesco Giorgino
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0057892
Abstract: Differences in the inherent properties of adipose tissue-derived stem cells (ASC) may contribute to the biological specificity of the subcutaneous (Sc) and visceral (V) adipose tissue depots. In this study, three distinct subpopulations of ASC, i.e. ASCSVF, ASCBottom, and ASCCeiling, were isolated from Sc and V fat biopsies of non-obese subjects, and their gene expression and functional characteristics were investigated. Genome-wide mRNA expression profiles of ASCSVF, ASCBottom and ASCCeiling from Sc fat were significantly different as compared to their homologous subsets of V-ASCs. Furthermore, ASCSVF, ASCCeiling and ASCBottom from the same fat depot were also distinct from each other. In this respect, both principal component analysis and hierarchical clusters analysis showed that ASCCeiling and ASCSVF shared a similar pattern of closely related genes, which was highly different when compared to that of ASCBottom. However, larger variations in gene expression were found in inter-depot than in intra-depot comparisons. The analysis of connectivity of genes differently expressed in each ASC subset demonstrated that, although there was some overlap, there was also a clear distinction between each Sc-ASC and their corresponding V-ASC subsets, and among ASCSVF, ASCBottom, and ASCCeiling of Sc or V fat depots in regard to networks associated with regulation of cell cycle, cell organization and development, inflammation and metabolic responses. Finally, the release of several cytokines and growth factors in the ASC cultured medium also showed both inter- and intra-depot differences. Thus, ASCCeiling and ASCBottom can be identified as two genetically and functionally heterogeneous ASC populations in addition to the ASCSVF, with ASCBottom showing the highest degree of unmatched gene expression. On the other hand, inter-depot seem to prevail over intra-depot differences in the ASC gene expression assets and network functions, contributing to the high degree of specificity of Sc and V adipose tissue in humans.
Stacks of trigonal curves
Michele Bolognesi,Angelo Vistoli
Mathematics , 2009,
Abstract: In this paper we give a presentation of the stack of trigonal curves as a quotient stack, and we compute its Picard group.
Portal hypertensive enteropathy diagnosed by capsule endoscopy and demonstration of the ileal changes after transjugular intrahepatic portosystemic shunt placement: a case report
Alessandro Pezzoli, Nadia Fusetti, Loredana Simone, Angelo Zelante, Viviana Cifalà, Alessandra Carella, Sergio Gullini
Journal of Medical Case Reports , 2011, DOI: 10.1186/1752-1947-5-90
Abstract: An 83-year-old Caucasian woman was admitted to our hospital for anemia and a positive fecal occult blood test. An upper gastrointestinal endoscopy revealed small varices without bleeding signs and hypertensive gastropathy. Colonoscopy was negative. To rule out any other cause of bleeding, capsule endoscopy was performed; capsule endoscopy revealed severe hyperemia of the jejunum-ileal mucosa with active bleeding. Because of the persistence of anemia and the frequent blood transfusions, not responding to β-blocker drugs or octreotide infusion, a transjugular intrahepatic portosystemic shunt was performed. Anemia improved quickly after the transjugular intrahepatic portosystemic shunt, and no further blood transfusion was necessary in the follow-up. The patient developed portal encephalopathy two months later and was readmitted to our department. We repeated the capsule endoscopy that showed a significant improvement of the gastric and ileal mucosa without any signs of bleeding.Hypertensive enteropathy is a rare condition, but it seems more common with the introduction of capsule endoscopy in clinical practice. This case shows that the jejunum can be a source of bleeding in cirrhosis patients, and this is the first demonstration of its resolution after transjugular intrahepatic portosystemic shunt placement.Changes in the gastric mucosa are a well-known aspect of cirrhosis in patients with portal hypertension [1]. Some data suggest that similar abnormalities can also occur in the duodenum, jejunum, and distal ileum [2-4]. With the introduction of capsule endoscopy (CE) in clinical practice, these changes can be detected easily, and the so-called "portal hypertensive enteropathy" [5] seems more common [6-9]. We present a case of portal hypertensive enteropathy (PHE) in a cirrhosis patient shown by CE and the effect of transjugular intrahepatic portosystemic shunt (TIPS) placement on the ileal pictures.An 83-year-old Caucasian woman with cirrhosis was admitted several t
The “Wireless Sensor Networks for City-Wide Ambient Intelligence (WISE-WAI)” Project
Paolo Casari,Angelo P. Castellani,Angelo Cenedese,Claudio Lora,Michele Rossi,Luca Schenato,Michele Zorzi
Sensors , 2009, DOI: 10.3390/s90604056
Abstract: This paper gives a detailed technical overview of some of the activities carried out in the context of the “Wireless Sensor networks for city-Wide Ambient Intelligence (WISEWAI)” project, funded by the Cassa di Risparmio di Padova e Rovigo Foundation, Italy. The main aim of the project is to demonstrate the feasibility of large-scale wireless sensor network deployments, whereby tiny objects integrating one or more environmental sensors (humidity, temperature, light intensity), a microcontroller and a wireless transceiver are deployed over a large area, which in this case involves the buildings of the Department of Information Engineering at the University of Padova. We will describe how the network is organized to provide full-scale automated functions, and which services and applications it is configured to provide. These applications include long-term environmental monitoring, alarm event detection and propagation, single-sensor interrogation, localization and tracking of objects, assisted navigation, as well as fast data dissemination services to be used, e.g., to rapidly re-program all sensors over-the-air. The organization of such a large testbed requires notable efforts in terms of communication protocols and strategies, whose design must pursue scalability, energy efficiency (while sensors are connected through USB cables for logging and debugging purposes, most of them will be battery-operated), as well as the capability to support applications with diverse requirements. These efforts, the description of a subset of the results obtained so far, and of the final objectives to be met are the scope of the present paper.
Modification of Cul1 regulates its association with proteasomal subunits
Joanna Bloom, Angelo Peschiaroli, George DeMartino, Michele Pagano
Cell Division , 2006, DOI: 10.1186/1747-1028-1-5
Abstract: We found that the binding of SCF complexes to proteasomes is conserved in higher eukaryotes. The Cul1 subunit associated with both sub-complexes of the proteasome, and high molecular weight forms of Cul1 bound to the 19S proteasome. Cul1 is ubiquitylated in vivo. Ubiquitylation of Cul1 promotes its binding to the S5a subunit of the 19S sub-complex without affecting Cul1 stability.The association of ubiquitylating enzymes with proteasomes may be an additional means to target ubiquitylated substrates for degradation.The significance of ubiquitylation for the proteasomal degradation of many proteins has been well established. Studies using cell-lines that are temperature-sensitive for the E1 ubiquitin-activating enzyme indicate that the bulk of proteasomally degraded proteins accumulate at the non-permissive temperature, suggesting that ubiquitylation is necessary for their proteolysis [1]. However, whether ubiquitylation is the sole signal for recognition and subsequent degradation of substrates by the 26S proteasome is still unclear. The S5a subunit of the 19S proteasome (called Rpn10 in yeast) has affinity for ubiquitin chains [2]; however, the19S subunit Rpt5/S6' has been shown to recognize ubiquitylated proteins [3]. Deletion of rpn10 in yeast does not disrupt the degradation of the majority of short-lived proteins [4], suggesting there is some redundancy in targeting ubiquitylated substrates for degradation. It is possible that additional proteasomal subunits are capable of recognizing ubiquitylated substrates, and/or other cellular factors may be involved in the delivery of substrates from the ubiquitylation machinery to the proteasome (reviewed in [5]).Several studies have shown that the ubiquitylation of specific substrates is coupled to degradation by the 26S proteasome through the interaction of their respective ubiquitin ligases (E3s) with the proteasome. In particular, two E3s in yeast, Ubr1 and Ufd4, have been shown to associate with subunits of the regul
Effect of different sperm concentrations on the post-thaw viability and motility of turkey spermatozoa cryopreserved by the pellet method
Nicolaia Iaffaldano,Angelo Manchisi,Mario Gambacorta,Michele Di Iorio
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2009.s2.760
Abstract: The effects of different prefreezing semen concentrations on post-thawing quality of turkey semen cryopreserved with the pellet method were investigated. Ten pooled semen samples were each divided into 6 subsamples and diluted with Tselutin extender to obtain a final concentration of 0.5, 1, 2, 3, 4 and 5x109/mL respectively. Subsamples were cooled, added with 8% of dimethylaceta- mide as cryoprotectant and, after 5 minutes of equilibration, seminal aliquots of 80 μL were directly dropped into a liquid nitrogen bath to form frozen pellets. Thawing was performed in few seconds at 75°C. Sperm motility (Accudenz swim-down test), viability (SyBr-Propidium Iodide staining) and sperm susceptibility to osmotic stress (Hyposmotic-water test) were assessed. Cryopreservation caused an overall loss of sperm quality, however differences in seminal parameters due to the different sperm concentration were observed in turkey spermatozoa after thawing: spermatozoa diluted to 4x109/mL showed significant higher values in mobility, viable and osmotic resistant spermatozoa compared to the other concentrations. This study showed that the post-thaw quality of turkey semen cryopreserved by pellets method was affected differently in relation to prefreezing sperm concentration.
Honeypot-powered Malware Reverse Engineering
Michele Bombardieri,Salvatore Castanò,Fabrizio Curcio,Angelo Furfaro
Computer Science , 2015,
Abstract: Honeypots, i.e. networked computer systems specially designed and crafted to mimic the normal operations of other systems while capturing and storing information about the interactions with the world outside, are a crucial technology into the study of cyber threats and attacks that propagate and occur through networks. Among them, high interaction honeypots are considered the most efficient because the attacker (whether automated or not) perceives realistic interactions with the target machine. In the case of automated attacks, propagated by malwares, currently available honeypots alone are not specialized enough to allow the analysis of their behaviors and effects on the target system. The research presented in this paper shows how high interaction honeypots can be enhanced by powering them with specific features that improve the reverse engineering activities needed to effectively analyze captured malicious entities.
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