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Search Results: 1 - 10 of 20504 matches for " Anderson Messias Rodrigues "
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Messias Rodrigues
Messias Rodrigues
Revista Dental Press de Ortodontia e Ortopedia Facial , 2005, DOI: 10.1590/s1415-54192005000600004
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Gregory Gegembauer,Leticia Mendes Araujo,Edy Firmina Pereira,Anderson Messias Rodrigues,Anamaria Mello Miranda Paniago,Rosane Christine Hahn,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002986
Abstract: Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
Uma estrutura de classifica??o com enfoque na cultura amaz?nica
Rodrigues, Anderson;
Ciência da Informa??o , 2005, DOI: 10.1590/S0100-19652005000200005
Abstract: this work intends to develop a classification framework that aims to create a thesaurus that will focus on the amazon culture of pará state. some narrative cultural terms were collected from chove nos campos de cachoeira, a novel by dalcídio jurandir. this novel was read with the intention of identifying, analyzing, selecting, defining and registering its cultural terms into a terminological log. after that, relationships among concepts were established, giving rise to classes from their common characteristics. a total of 512 cultural terms were collected from the narrative and 912 conceptual unities emerged (including classes, subclasses, equivalent and related terms). taking into account that all organized information has an expectation of generating new knowledge, it is believed that the production of this thesaurus may be helpful to preserve amazon cultural memory.
Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks
Anderson Messias Rodrigues,Marcus de Melo Teixeira,G. Sybren de Hoog,Tania Maria Pacheco Schubach,Sandro Antonio Pereira,Geisa Ferreira Fernandes,Leila Maria Lopes Bezerra,Maria Sueli Felipe,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002281
Abstract: Sporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. In order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1α) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Paraná (PR, n = 4), S?o Paulo (SP, n = 3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. The genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. The occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil.
Proteomics-Based Characterization of the Humoral Immune Response in Sporotrichosis: Toward Discovery of Potential Diagnostic and Vaccine Antigens
Anderson Messias Rodrigues,Geisa Ferreira Fernandes?,Leticia Mendes Araujo?,Paula Portella Della Terra?,Priscila Oliveira dos Santos?,Sandro Antonio Pereira?,Tania Maria Pacheco Schubach?,Eva Burger?,Leila Maria Lopes-Bezerra?,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0004016
Abstract: Background Sporothrix schenckii and associated species are agents of human and animal sporotrichosis that cause large sapronoses and zoonoses worldwide. Epidemiological surveillance has highlighted an overwhelming occurrence of the highly pathogenic fungus Sporothrix brasiliensis during feline outbreaks, leading to massive transmissions to humans. Early diagnosis of feline sporotrichosis by demonstrating the presence of a surrogate marker of infection can have a key role for selecting appropriate disease control measures and minimizing zoonotic transmission to humans. Methodology We explored the presence and diversity of serum antibodies (IgG) specific against Sporothrix antigens in cats with sporotrichosis and evaluated the utility of these antibodies for serodiagnosis. Antigen profiling included protein extracts from the closest known relatives S. brasiliensis and S. schenckii. Enzyme-linked immunosorbent assays and immunoblotting enabled us to characterize the major antigens of feline sporotrichosis from sera from cats with sporotrichosis (n = 49), healthy cats (n = 19), and cats with other diseases (n = 20). Principal Findings Enzyme-linked immunosorbent assay-based quantitation of anti-Sporothrix IgG exhibited high sensitivity and specificity in cats with sporotrichosis (area under the curve, 1.0; 95% confidence interval, 0.94–1; P<0.0001) versus controls. The two sets of Sporothrix antigens were remarkably cross-reactive, supporting the hypothesis that antigenic epitopes may be conserved among closely related agents. One-dimensional immunoblotting indicated that 3-carboxymuconate cyclase (a 60-kDa protein in S. brasiliensis and a 70-kDa protein in S. schenckii) is the immunodominant antigen in feline sporotrichosis. Two-dimensional immunoblotting revealed six IgG-reactive isoforms of gp60 in the S. brasiliensis proteome, similar to the humoral response found in human sporotrichosis. Conclusions A convergent IgG-response in various hosts (mice, cats, and humans) has important implications for our understanding of the coevolution of Sporothrix and its warm-blooded hosts. We propose that 3-carboxymuconate cyclase has potential for the serological diagnosis of sporotrichosis and as target for the development of an effective multi-species vaccine against sporotrichosis in animals and humans.
The Difference of the Impact of Elective Cholecystectomy Surgery in the Immune Response  [PDF]
Rosamaria Rodrigues Gomes, Manoel Messias Lima Neto, Ayrton Silva Alves, Cristiane Monteiro da Cruz
Surgical Science (SS) , 2018, DOI: 10.4236/ss.2018.98029
Abstract: Introduction: The inflammatory response is essential to initiate the healing process, and in this response there is an increase in white blood cells and pro-inflammatory compounds. Creactive proteins (CRP), together with the blood leukocyte count, have been used to study the immune response. Due to the risk of infection and exacerbated inflammatory response of the patients undergoing surgical procedure, it is relevant to investigate the pre and postoperative inflammatory response of those individuals. Objective: To determine the difference in the impact of elective cholecystectomy surgery on the patient’s immune response. Methods: The present study was cross-sectional, where a group included patients who underwent elective cholecystectomy procedure. Patients who were pregnant, under 18 years, of indigenous communities or with other conditions that depress the immune system, as well as those on immunosuppressive medications, were excluded. Results: CRP and leukocyte counts indicated a mean increase of 5.97-fold (95% CI 3.76 to 8.34, SD 6.98) and 1.97-fold (95% CI 1.71 to 2.24, SD 1.97) respectively, both being evaluated before and after surgery. Discussion: In the present study, to avoid a possible methodological bias, it was chosen to evaluate the patients submitted to videolaparoscopic cholecystectomy only and the hypothesis was confirmed that there is an increase in CRP and leukocyte count as a response to surgical trauma. Conclusion: There was an elevation of the inflammatory markers in patients submitted to surgical trauma when comparing the exams collected in the preoperative period and after the surgical aggression.
A sele??o conceitual na organiza??o de domínios de conhecimento nas ciências humanas e sociais: o caso da cultura
Rodrigues, Anderson Luiz Cardoso;
Perspectivas em Ciência da Informa??o , 2011, DOI: 10.1590/S1413-99362011000200009
Abstract: this paper appproaches the conceptual selection in the organization of knowledge domains in the human and social science is approached, focusing on the culture concept and its peculiar complexity. conceptual selection is an initial stage in system construction in the organization of knowledge and its importance is shown for a final quality result regarding the recovery of information. to show the complexity of the culture domain, the etymological origins of amazon words are analyzed, where a wide cultural diversity in original non-native african, european and asian languages. the theory of the faceted classification by ranganathan and the theory of the integrated levels from the classification research group were suggested as theorical and methodological basis to deal with complex domains of this nature.
A Complexidade da cultura amaz nica e seus reflexos para a organiza o e representa o da informa o
Anderson Luiz Cardoso Rodrigues
AtoZ : Novas Práticas em Informa??o e Conhecimento , 2012,
Abstract: Introdu o: Explora e analisa o domínio ‘cultura amaz nica’ no contexto da organiza o e representa o da informa o. Apresenta conceitos de cultura com o objetivo de embasar as reflex es sobre o domínio estudado.Método: O referencial teórico de apoio é centrado na Teoria da Classifica o Facetada de Ranganathan e na Teoria dos Níveis Integrativos do Classification Research Group, como base teórico-metodológica para a elabora o de um modelo de estrutura classificatória. A sele o dos conceitos foi realizada tomando por base os termos resultantes de pesquisa oral de três localidades geográficas da Amaz nia: as cidades de Bragan a, Castanhal e o arquipélago do Marajó. Resultados: A análise das origens etimológicas das palavras indicou a influência das culturas estrangeiras dos continentes europeu, africano, asiático e americano na linguagem amaz nica. Conclus es: A análise dos resultados revelou a viabilidade de se desenvolver uma metodologia de trabalho para delinear uma estrutura de classifica o e de um conjunto de conceitos para representar o domínio ‘cultura amaz nica’ nas suas raízes basilares como estrutura para a constru o de sistemas de organiza o do conhecimento.
Bragantia , 1998, DOI: 10.1590/S0006-87051998000200005
Abstract: the snap bean cultivars alessa, hab 52 e hab 198 and dry bean breeding lines bac-6 and a-794 and their diallel crosses were evaluated for pod number, seed number, pod lenght, pod diameter, fiber content and plant height. these evaluations were made per plant and diallel analysis was conducted using griffing's model. general combining ability (gca) was significant for all characters evaluated. specific combining ability (sca) was significant for pod diameter, fiber content and plant height. bac-6, a-794 and 'alessa' were considered superior genotypes for pod number per plant, seed number per plant, pod lenght and fiber content. non-additive effects were predominant in pod reaction and plant height. the best combinations were 'alessa' x bac-6, 'alessa' x a-794 and 'hab 52' x bac-6.
Bragantia , 1998,
Abstract: Os cultivares de feij o-de-vagem Alessa, Hab 52 e Hab 198, as linhagens de feij o comum Bac-6 e A-794 e os dez possíveis híbridos F1s dialélicos foram avaliados quanto ao número de vagens e de sementes por planta, comprimento, diametro e teor de fibra da vagem e altura de planta. A análise dialélica foi feita com base no método II, modelo 1 de Griffing. A capacidade geral de combina o foi significativa para todos os caracteres avaliados. Efeitos significativos para a capacidade específica de combina o foram observados para diametro e teor de fibra da vagem e altura de planta. Constatou-se que os efeitos de aditividade foram superiores aos de dominancia para número de vagens e de sementes por planta, comprimento e teor de fibra da vagem, identificando-se os genótipos Bac-6, A-794 e 'Alessa' como os mais promissores. Para os demais caracteres, os efeitos de dominancia se sobrepuseram aos de aditividade, permitindo-se destacar as combina es 'Alessa' x Bac-6, 'Alessa' x A-794 e 'Hab 52' x Bac-6.
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