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Search Results: 1 - 10 of 36501 matches for " Ana Raquel Mano Meinerz "
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CANDIDíASE CUT NEA EM CEBUS APELLA (MACACO PREGO) CUTANEOUS CANDIDIASIS IN A CEBUS APELLA (CAPUCHINS MONKEYS)
Marlete Brum Cleff,Mauro Pereira Soares,Isabel Martins Madrid,Ana Raquel Mano Meinerz
Ciência Animal Brasileira , 2008,
Abstract: Leveduras do gênero Candida têm sido freqüentemente isoladas de animais domésticos e silvestres, entretanto, candidíase n o tem sido reportada em primatas. Encaminhou-se à Faculdade de Veterinária, Departamento de Patologia Animal, um macaco-prego (Cebus apella) para necropsia, que vinha apresentando emagrecimento profundo e les es ulcerativas de pele e mucosas. Fragmentos de pele e órg os foram processados para histologia e corados com H.E. e Groccot. Para micologia, coletaram-se fragmentos de órg os, exsudato e crostas da pele, sendo realizado exame direto e cultivo a 37oC. Macroscopicamente, o animal apresentava alopecia, caquexia e ulcera es cutaneas de 1-4 cm. Histologicamente, nas ulcera es, a derme continha infiltrado de mononucleares e prolifera o fibroblástica. Mediante utiliza o de Groccot, encontraram-se hifas e/ou pseudo-hifas e blastoconídeos intralesionais. Em cultivos de crostas e exsudato, observaram-se col nias brilhantes, com superfície lisa e colora o branca a creme. A microscopia das col nias revelou células leveduriformes ovaladas ou alongadas, com brotamento unipolar, gram-positivas. No microcultivo em fubá, observaram-se blastoconídeos globosos terminais, com parede espessa e pseudomicélio abundante e ramificado, com forma o de tubo germinativo em albumina de ovo, sendo a levedura classificada como Candida albicans. O estudo aborda o risco da infec o por micoses oportunistas como a candidíase em animais silvestres em cativeiro. PALAVRAS-CHAVES: Candidiasis, Candida albicans, pele, Cebus apella. Candida spp has been frequently isolated from domestic and sylvan animals, however, Candidiasis has not been reported in primates. One Cebus apella, with progressive thinning and ulcerative skin lesions and mucous, was necropsied. Fragments of tissue were collected and processed for histology. Coloration of H.E. and Groccot was made. For mycology was collected tissue, exsudate, and skin crusts. Direct exam was made, and samples cultivated at 37oC in agar Sabouraud with cloranfenicol and agar corn. Macroscopically the animal presented; itself extremely thin with ulcerative lesions on the skin, ranging from 1 to 4 cm. Histologically, on dermis ulcerations, there was mononuclear infiltrate and fibroblastic proliferation. With Groccot, it was observed intralesionally hyphae and blastoconidia. The direct exam showed round and/or ovalate gram positive cells, compatible with yeast. The cultives showed shining colonies with a smooth surface, some of them with edges fringed and white-yellowish coloration. The colonies showed yeast cells wit
ESTUDO DA FREQUêNCIA DE Malassezia pachydermatis EM C ES COM OTITE EXTERNA NO RIO GRANDE DO SUL
Patrícia da Silva Nascente,Rosema Santin,Ana Raquel Mano Meinerz,Anelise Afonso Martins
Ciência Animal Brasileira , 2010,
Abstract: External otitis is a common auditory meatus infirmity diagnosed in dogs and cats sent to veterinarian clinics. The etiology varies according to combinations of predisposing, primary and perpetuating factors, responsible for the disease. Malassezia pachydermatis is considered a normal inhabitant of the skin and can occasionally become an opportunistic pathogen of the external canal of dogs and cats. This study aimed to investigate the presence of M. pachydermatis in canine external otitis. The presence of yeast in external otitis of dogs sent to 168 veterinary hospitals in the Federal Universities of Rio Grande do Sul and Pelotas, as well as to clinics and private kennels was investigated. The sampling was performed with a sterile swab moistened with saline solution, rubbing it in the ear canal. Direct examination was performed from swabs of the collected material, stained by Gram method and examined under optical microscopy (1000X). It was seeded on Sabouraud Dextrose Agar with chloramphenicol and incubated at 32°C for up to ten days when the yeasts were identified. From the 168 cases of external otitis, M. pachydermatis was isolated in 139 (82.7%) cases. In this study, there was no difference regarding gender and age of the animals. The frequency of isolation in samples with positive scores on direct examination was significantly higher (p <0.05) than in samples with negative score. It was verified that there is a seven times-higher probability of isolating M. pachydermatis from samples with positive direct examination. It was concluded that M. pachydermatis is commonly isolated in canine external otitis cases, without significant difference in relation to sex and age. Animals with ear shifts (eg. English Cocker Spaniel) are more affected by external otitis; however, there is no difference in proportion of the number of isolation of M. pachydermatis among the races.
Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR = Heterogeneidade molecular da Malassezia pachydermatis através de RAPD-PCR
Patrícia da Silva Nascente,Helen Silveira Coimbra,Ana Raquel Mano Meinerz,Mário Carlos Araújo Meireles
Acta Scientiarum : Biological Sciences , 2010,
Abstract: Several methodologies in molecular biology have been used in theinvestigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiologicalstudy of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A variation was observed in the number and arrangement of the bands among the 49 studied isolates, grouped into nine patterns. Isolate groupings were not found to be related to animal breed, age or sex. It was concluded that M. pachydermatis has differences in its molecular profile, as shown by the molecular technique (RAPD – PCR), which allows isolates to be classified into nine subtypes. Várias metodologias em biologia molecular têm sido aplicadas para estudar a M. pachydermatis diferenciando-a em subgrupos. Recentemente utiliza-se a investiga o molecular desta espécie isolada de otite externa e dermatite, e também de isolados da mesma de animais hígidos, para um estudo epidemiológico da levedura. O objetivo deste trabalho foi identificar diferen as moleculares entre isolados de M. pachydermatis obtidos de casos de otite externa canina. Para isto, amostras da levedura provenientes de c es com esta enfermidade foram estudadas através da técnica de Polimorfismo de DNA Amplificado aoAcaso - Rea o da Polimerase em Cadeia (RAPD–PCR) para pesquisa de heterogeneidade molecular. A extra o de DNA foi realizada no processo fenol-cloroformio e a técnica de RAPD foi estudada com o primer AGAATCCGCC. Pode-se observar com esta metodologia, varia o no número e posi o das bandas entre os 49 isolados estudados, podendo-se agrupá-los em nove padr es. Os agrupamentos formados pelos isolados n o apresentaram rela o com ra a, idade ou sexo do animal. Concluindo-se que a M. pachydermatis apresenta diferen as em seu perfil molecular, observado pela técnica molecular (RAPD-PCR) que permite classificar os isolados desta espécie em até nove subtipos.
FREQUENCY OF THE VIRUS OF THE FELINE LEUKEMIA (FeLV) IN DOMESTIC FELINES (Felis catus) SEMI-DOMICILED IN THE MUNICIPALITIES OF PELOTAS AND RIO GRANDE FREQUêNCIA DO Vírus da Leucemia Felina (VLFe) em FELINOS DOMéSTICOS (Felis catus) SEMIDOMICILIADOS NOS MUNICíPIOS DE PELOTAS E RIO GRANDE
Ana Raquel Mano Meinerz,Tatiana de ávila Antunes,Lorena Leonardo de Souza,Patrícia da Silva Nascente
Ciência Animal Brasileira , 2010,
Abstract: Considering the importance of FeLV in the feline clinic, as well as the likely agent spread from a symptomatic or asymptomatic feline bearer, this work has as objective the study of the frequency of FeLV in felines residents in the cities of the Pelotas and Rio Grande, municipalities located in the south area of Brazil. For that, the blood of 120 semi-domiciled animals was collected for the detection of the retrovirus through the Indirect Immunofluorescence technique (IFA). FeLV was detected in 38,3% (46/120) of the studied animals, representing a larger frequency considering other studies accomplished in other areas of Brazil, what confirms the importance of FeLV in the studied region. KEY WORDS: FeLV, felines, immunofluorescence, retrovirus. Considerando a importancia do VLFe na clínica felina, assim como a possível dissemina o do agente a partir de um felino portador sintomático ou assintomático, o estudo tem como objetivo verificar a frequência de viremia pelo VLFe em felinos residentes em Pelotas e Rio Grande, municípios situados na regi o sul do Brasil. Para isso foi coletado sangue de 120 animais semidomiciliados para a detec o do retrovírus através da técnica de imunofluorescência indireta (IFI). Detectou-se a viremia em 38,3% (46/120) dos animais estudados, representando uma frequência maior em rela o a outros estudos realizados no Brasil, o que confirma a importancia deste agente na regi o estudada. PALAVRAS-CHAVES: Felinos, imunofluorescência, retrovírus, VLFe.
AVALIA O DOS MéTODOS DE ETEST E MICRODILUI O EM CALDO PARA O ESTUDO DA SUSCETIBILIDADE DO Sporothrix schenckii COM O ITRACONAZOL
Ana Raquel Mano Meinerz,Marlete Brum Cleff,Patrícia da Silva Nascente,Luiza da Gama Osório
Ciência Animal Brasileira , 2010,
Abstract: The frequent occurrence of resistant isolated fungi againstantifungal drugs stimulated advances in the antifungigram techniques,which were standardized by CLSI. However, the methods have been inefficient and impractical to be executed in clinical laboratories. Within this context, commercial techniques have been developed, being ETEST one of them. ETEST has proved to be easier to execute when compared to the techniques approved by the CLSI. This study used the ETEST and the microdilution method, performed according to CLSI, for determining the in vitro susceptibility of isolates of Sporothrix schenckii against itraconazole. The CLSI uses RPMI 1640 medium and the reading of MIC after the period of incubation of 72h at 35oC. MIC was determined by the ETEST, being Sabouraud dextrose agar used as medium, and the reading performed after 72 hours of incubation at 35oC. The variance analysis, analyzed by T-paired test, did not demonstrate statistical differences among the CIM values obtained by the microdilution technique in broth (MIC among 0.219 and 0.875 μg/mL) and ETEST (MIC among 0.032 and 2.0 μg/mL). However, the correlation coefficient (R) was negative, probably because ofthe small number of samples. These results show the necessity offurther studies to assess the application of ETEST to evaluate thesusceptibility of S. schenckii against the itraconazol.
ESPOROTRICOSE FELINA: RELATO DE CASOS SCIENTIFIC REPORT:FELINE SPOROTRICHOSIS – CASE REPORTS
Ana Raquel Mano Meinerz,Patrícia da Silva Nascente,Luiz Filipe Damé Schuch,Renata Osório de Faria
Ciência Animal Brasileira , 2007,
Abstract: o felino doméstico com esporotricose representa um papel importante na transmiss o do S. schenckii a ou-tros animais e para o homem sendo crescente o aumento do número de casos zoonóticos da micose. o estudo des-creve três casos de esporotricose em gatos semidomicilia-dos, convivendo no mesmo bairro. os felinos apresenta-ram les es cutaneas ulceradas e crostosas, sendo realizada a confirma o do diagnóstico após a análise micológica com o isolamento do S. schenckii. o estudo ressalta a im-portancia dos felinos domésticos como propagadores da esporotricose para animais e para o homem. PALAVRAS-CHAVE: Esporotricose, zoonose, felino, Sporothrix schenckii. the domestic feline with sporotrichosis represents an important role in the transmission of the S. schenckii schenckii to other animal species and for the man being growing the increase of the number of zoonotic cases of the mycosis. the study reports three cases of feline sporo-trichosis in semi-domiciled cats, living together in the same neighborhood. the feline presented cutaneous ulcerated and crusted injuries being accomplished the confirmation of the diagnosis after the analysis micológica with the isolation of S. schenckii. the study emphasizes the importance of the domestic feline as diffuser of the sporotrichosis for other animals and for the man itself. KEY-WORDS: feline, sporotrichosis, zoonosis, Sporothrix schenckii.
Isolation of Sporothrix schenkii from the nails of healthy cats
Souza, Lorena Leonardo de;Nascente, Patrícia da Silva;Nobre, Márcia Oliveira;Meinerz, Ana Raquel Mano;Meireles, Mário Carlos Araújo;
Brazilian Journal of Microbiology , 2006, DOI: 10.1590/S1517-83822006000300031
Abstract: many cases of sporotrichosis in cats were diagnosed in the town of rio grande, rs, brazil, caused mainly by inoculation due to scratches. in this work, we evaluated the frequency of sporothrix schenckii in the nails of healthy cats living together with cats showing clinical sporotrichosis. the isolation of the fungus was carried out pressing unwashed nails of the forelimbs of 24 cats (48 paws) directly onto the surface of mycobiotic agar in petri dishes. s. schenckii was isolated from seven (29.1%) cats. the procedure for isolation of the fungus was simpler than methods that require sedation and cuts of the nails.
CLSI broth microdilution method for testing susceptibility of Malasseziapachydermatis to thiabendazole
Nascente, Patrícia da Silva;Meinerz, Ana Raquel Mano;Faria, Renata Osório de;Schuch, Luiz Filipe Damé;Meireles, Mário Carlos Araújo;Mello, Jo?o Roberto Braga de;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000200002
Abstract: thiabendazole, classified as antiparasitic and also used as an antifungal drug, can be found as otological solution indicated for treatment of parasitic and fungal external otitis in small animals. malassezia pachydermatis is a yeast recognized as a normal inhabitant on the skin and mucous membranes of dogs and cats. however, it is considered an opportunistic agent that causes external otitis and dermatitis in these animals. the aim of this study was to evaluate the in vitro effect of thiabendazole against 51 isolates of m. pachydermatis using the clsi broth microdilution method that has been adapted for this yeast species (nccls, 2002). based on this test, the minimum inhibitory concentrations (mic) of thiabendazol was calculated. subsequently, the susceptibility of each isolate against this antifungal was determined. it was observed that the mic of thiabendazole against m. pachydermatis ranged from 0.03 to > 4 μg/ml. a total of 13.7% of the isolates were found to be resistant, 47.1% were intermediate and 39.2% were sensitive to the drug. the rate of resistance of the yeasts against thiabendazole was similar to the results previously obtained with other antifungals, while the adapted broth microdilution technique used in this study proved to be efficient.
Efficacy of terbinafine and itraconazole on a experimental model of systemic sporotrichosis
Meinerz, Ana Raquel Mano;Xavier, Melissa O.;Cleff, Marlete Brum;Madrid, Isabel Martins;Nobre, Márcia Oliveira;Meireles, Mário Carlos Araújo;Mello, Jo?o Roberto Braga de;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000400025
Abstract: itraconazole is currently considered the drug of choice to treat the diverse clinical presentation of sporotrichosis. on the other hand terbinafine by virtue of its excellent in vitro activity is under comparative evaluation for its therapeutic potential for a wide range of fungal infections. in this study, our aim was to determine the in vivo efficacy of terbinafine and itraconazole on a experimental model of systemic sporotrichosis. 120 rats wistar received an injection of 2x103 s. schenckii cells by via the lateral tail vein. after 3 days the animals were treated with terbinafine (250mg/kg) and itraconazole (100 mg/kg) and their respective diluents. in our model, terbinafine and itraconazole were effective in reducing the number of clinical lesions and positive organ cultures. there was statistical difference between the groups treated with the antifungals in relation to the control groups (p<0,05) concerning the clinical alterations, anatomic-pathological findings and in the positive organ cultures of the agent, being that the treated animals resulted in the absence and/or reduction of all the evaluated parameters. as for the treatments, terbinafine showed similar or higher activity that itraconazole in the evaluation of the testicle alteration (p=0,0004), as well as in the positive organ cultures of microorganism from the organ (p=0,0142). with these results it is possible to conclude that the antifungals studied are effective in the treatment of experimental systemic sporotrichosis.
Efeitos de doses elevadas da terbinafina e itraconazol em ratos Wistar
Meinerz, Ana Raquel Mano;Cleff, Marlete Brum;Nascente, Patrícia da Silva;Nobre, Márcia de Oliveira;Schuch, Luiz Filipe Damé;Antunes, Tatiana de ávila;Xavier, Melissa O.;Meireles, Mário Carlos Araújo;Mello, Jo?o Roberto de Braga;
Revista Brasileira de Ciências Farmacêuticas , 2007, DOI: 10.1590/S1516-93322007000100013
Abstract: taking into account the importance of the itraconazole and terbinafine in the antifungal therapy, the study has as objective evaluate the effects of high itraconazole doses (100 mg/kg) and terbinafine (250 mg/kg) in rats albino wistar through the analyses of the hepatic enzymes (alt and alp), complete blood count and histopathologic study of different organs. the drugs were administered orally once a day, for a period of 30 days, when blood was collected and accomplished the necropsy of the experimental animals. the detected values of the hepatic enzymes and of the blood count were compatible to the physiologic indexes for the studied species with no statistical differences among the experimental groups. the histopathologic exam did not reveal any abnormality, however 25% of the treated with terbinafine died immediately after the administration of the drug. with those results we concluded that the administered doses of the drugs did not alter the appraised hepatic enzymes, as well as the blood count. however, more studies are needed to consider high doses of the terbinafine and itraconazole as viable treatment alternative for systemic mycosis.
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