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Search Results: 1 - 10 of 428322 matches for " Ana M. Amor Vigil "
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Importancia del estudio del quimerismo en el trasplante alogénico de médula ósea
Amor Vigil,Ana M; Martínez Antu?a,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2003,
Abstract: since the hematopoietic transplantation was performed for the first time, the importance of knowing chimerism was underlined. the present paper updates the concepts of chimerism, its classification and the different ways to determine it. the most recommended is the highly polymorphic area amplification in the dna using the polymerase chain reaction technique. this technique has allowed carrying out studies on the evolution of chimera, to relate the set level of chimerism with the graft behaviour and the graft-versus-host disease under different conditioning. it has also made it possible to early detect relapse of transplanted patients and the prompt administration of additional immunotherapy. finally, the paper presents the recommendations of the american society of blood and bone marrow transplantation for standardizing the study of chimerism in the therapy centers
Importancia del estudio del quimerismo en el trasplante alogénico de médula ósea IMPORTANCE OF THE STUDY OF CHIMERISM IN THE ALLOGENEIC BONE MARROW TRANSPLANTATION
Ana M Amor Vigil,Gisela Martínez Antu?a
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2003,
Abstract: Desde que se comenzó a realizar el trasplante hematopoyético se reconoció la importancia de conocer el establecimiento de quimerismo. El presente trabajo actualiza los conceptos de quimerismo, su clasificación y las diferentes vías para su determinación. Se destaca como la más recomendada, la amplificación de zonas altamente polimórficas en el ADN por la técnica de la reacción en cadena de la polimerasa. La utilización de esta técnica ha permitido realizar estudios de la evolución de la quimera, relacionar el grado de quimerismo establecido con el comportamiento del injerto y de la enfermedad de injerto contra hospedero en los diferentes regímenes de acondicionamiento. También ha posibilitado la detección precoz de la recaída en los pacientes trasplantados y la administración oportuna de inmunoterapia adicional. Finalmente, se presentan las recomendaciones de la Sociedad Americana de Trasplante de Sangre y Médula ósea para estandarizar el estudio del quimerismo en los diferentes centros de tratamiento Since the hematopoietic transplantation was performed for the first time, the importance of knowing chimerism was underlined. The present paper updates the concepts of chimerism, its classification and the different ways to determine it. The most recommended is the highly polymorphic area amplification in the DNA using the polymerase chain reaction technique. This technique has allowed carrying out studies on the evolution of chimera, to relate the set level of chimerism with the graft behaviour and the graft-versus-host disease under different conditioning. It has also made it possible to early detect relapse of transplanted patients and the prompt administration of additional immunotherapy. Finally, the paper presents the recommendations of the American Society of Blood and Bone Marrow Transplantation for standardizing the study of chimerism in the therapy centers
Diagnóstico de linfomas cutáneos mediante detección de clonalidad por PCR-heterodúplex
Amor Vigil,Ana M.; Matos Borges,Rafael; Martínez Antu?a,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: the clonicity detection in the lymphoproliferative syndromes by studying the rearrangement of the immunoglobulin genes and of the t-receptor cells is used to make clear if a proliferation or infiltrate of lymphocytes is malignant or not. this type of study is particularly useful in the presence of cutaneous lesions whose lymphoid or dermatological origin is difficult to define. by the pcr-heteroduplex technique, the genes of the immunoglobulin heavy chain and of the t-cell receptor chain were studied in 10 patients that presented dermatological manifestations attributable to some kind of cutaneous lymphoma. clonal rearrangement was observed in 7 patients, which allowed to confirm the diagnosis of mycosis fungoides and other types of cutaneous lymphomas. it was not possible to confirm a lymphoid process of malignant character by this technique in 3 patients who did not show clonal rearrangement. the usefulness of the study was proved when in the presence of a skin affection, it was difficult to differentiate a dermatological process from a proliferative syndrome with cutaneous manifestations.
Diagnóstico de linfomas cutáneos mediante detección de clonalidad por PCR-heterodúplex Diagnosis of cutaneous lymphomas by clonicity detection through PCR-heteroduplex technique
Ana M. Amor Vigil,Rafael Matos Borges,Gisela Martínez Antu?a
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: La detección de clonalidad en los síndromes linfoproliferativos mediante el estudio del reordenamiento de los genes de las inmuglobulinas y del receptor de células T, es utilizada para esclarecer si una proliferación o infiltrado de linfocitos es maligno o no. Este tipo de estudio es de particular utilidad en presencia de lesiones cutáneas cuyo origen linfoide o dermatológico resulta difícil de definir. Mediante la técnica de PCR-heterodúplex se estudiaron los genes de la cadena pesada de las inmunoglobulinas y de la cadena gamma del receptor de las células T, en 10 pacientes que presentaban manifestaciones dermatológicas atribuibles a algún tipo de linfoma cutáneo. Se observó reordenamiento clonal en 7 pacientes, lo cual permitió confirmar el diagnóstico de micosis fungoide y otros tipos de linfomas cutáneos. En 3 pacientes que no mostraron reordenamiento clonal, no fue posible confirmar por esta técnica un proceso linfoide de carácter maligno. Se demostró la utilidad del estudio cuando en presencia de una afección en la piel, es difícil diferenciar un proceso dermatológico de un síndrome linfoproliferativo con manifestaciones en piel. The clonicity detection in the lymphoproliferative syndromes by studying the rearrangement of the immunoglobulin genes and of the T-receptor cells is used to make clear if a proliferation or infiltrate of lymphocytes is malignant or not. This type of study is particularly useful in the presence of cutaneous lesions whose lymphoid or dermatological origin is difficult to define. By the PCR-heteroduplex technique, the genes of the immunoglobulin heavy chain and of the T-cell receptor chain were studied in 10 patients that presented dermatological manifestations attributable to some kind of cutaneous lymphoma. Clonal rearrangement was observed in 7 patients, which allowed to confirm the diagnosis of mycosis fungoides and other types of cutaneous lymphomas. It was not possible to confirm a lymphoid process of malignant character by this technique in 3 patients who did not show clonal rearrangement. The usefulness of the study was proved when in the presence of a skin affection, it was difficult to differentiate a dermatological process from a proliferative syndrome with cutaneous manifestations.
Transcripto BCR-ABL atípico en un paciente con leucemia mieloide crónica Atypical BCR-ABL transcript in a patient with chronic myeloid leukemia
Ana María Amor Vigil,Viviana Cristo Pérez,Yarienis González Medina,Ania Hernández Cabezas
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: Se presenta un paciente con leucemia mieloide crónica (LMC) y un transcripto BCR-ABL atípico. Mediante el análisis cualitativo de los productos de la reacción en cadena de la polimerasa, y por comparación con los pesos moleculares del producto de amplificación de muestras de pacientes positivos a los puntos de ruptura más frecuentes e13a2 (b2a2) y e14a2 (b3a2), se determinó que el gen quimérico BCR-ABL de este paciente debe corresponder al transcripto e13a3 (b2a3) que aparece con muy poca frecuencia en la LMC. El paciente se encuentra en tratamiento con imatinib (400 mg diarios) desde el inicio de la enfermedad. A los 6 meses no se detectó presencia del gen quimérico BCR-ABL; 2 estudios moleculares posteriores, al a o y a los 2 a os de tratamiento, resultaron positivos, pero esto no implicó modificación del tratamiento. La evolución favorable del paciente, a pesar de la recaída molecular, concuerda con lo esperado descrito en la literatura en relación con la presencia del transcripto b2a3 en la LMC. The case of a patient with chronic myeloid leukemia and atypical BCR-ABL transcript was presented. By using the qualitative analysis of the polymerase chain reaction products and the comparison with the molecular weights of the amplification product from the samples taken from those patients positive to the most frequent rupture points e13a2 (b2a2) and e14a2 (b3a2), it was determined that chimeric gen BCR-ABL of this patient should correspond to transcript e13a3 (b2a3) that rarely occurs in CML. The patient is being treated with Imatinib (400 mg daily) since the onset of his disease. After six months, the chimeric gen BCR-ABL was not detected. Two molecular studies carried out one year and two years after the treatment yielded positive results, but this did not imply any change to the treatment. Despite the molecular relapse, the favorable recovery of the patient agreed with the expected result described in the literature in terms of the presence of transcript b2a3 in CML.
Transcripto BCR-ABL atípico en un paciente con leucemia mieloide crónica
Amor Vigil,Ana María; Cristo Pérez,Viviana; González Medina,Yarienis; Hernández Cabezas,Ania; Pavón Morán,Valia; Martínez Antu?a,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: the case of a patient with chronic myeloid leukemia and atypical bcr-abl transcript was presented. by using the qualitative analysis of the polymerase chain reaction products and the comparison with the molecular weights of the amplification product from the samples taken from those patients positive to the most frequent rupture points e13a2 (b2a2) and e14a2 (b3a2), it was determined that chimeric gen bcr-abl of this patient should correspond to transcript e13a3 (b2a3) that rarely occurs in cml. the patient is being treated with imatinib (400 mg daily) since the onset of his disease. after six months, the chimeric gen bcr-abl was not detected. two molecular studies carried out one year and two years after the treatment yielded positive results, but this did not imply any change to the treatment. despite the molecular relapse, the favorable recovery of the patient agreed with the expected result described in the literature in terms of the presence of transcript b2a3 in cml.
Frecuencia de los transcriptos BCR-ABL en pacientes cubanos con leucemia mieloide crónica Frequency of BCR-ABL transcripts in Cuban patients with chronic myeloid leukemia
Ana María Amor-Vigil,Yarienis González-Medina,Niubys Cayado-Gutiérrez,Gisela Martínez-Antu?a
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2012,
Abstract:
Resultados relevantes en biología molecular, enzimopatías y citogenética en el período 1996-2010 Relevant results in molecular Biology, enzymopathies and citogenetics during 1996-2010
Ana María Amor-Vigil,Gisela Martínez-Antu?a,Kalia Lavaut-Sánchez,Graciela Pérez-Diez de los Ríos
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: El Departamento de Biología Celular y Molecular del Instituto de Hematología e Inmunología incluye 4 áreas: biología molecular, hemoglobinopatías, enzimas y membrana eritrocitaria y citogenética. Se presentan aspectos relevantes del trabajo desarrollado en los últimos a os que comprende investigaciones en las talasemias que permitieron conocer las bases moleculares de estas hemoglobinopatías en nuestra población. En el campo de la biología molecular se desarrolló inicialmente la técnica del southern blot; posteriormente, la de la reacción en cadena de la polimerasa y la transcripción inversa, que han sido muy útiles en el diagnóstico y seguimiento de pacientes con hemopatías malignas. La presencia de alteraciones citogenéticas (monosomías, trisomías, translocaciones, inversiones y deleciones) ha sido ampliamente evaluada en enfermedades hematológicas, con gran utilidad también en el diagnóstico, pronóstico y seguimiento de los pacientes. En cuanto a las enzimopatías, se pudo conocer la alteración molecular de variantes deficientes de glucosa-6-fosfato deshidrogenasa y con el desarrollo de las técnicas para el estudio de las proteínas de la membrana eritrocitaria, se determinó la alteración bioquímica de enfermos con esferocitosis hereditaria (EH). The Department of Cellular and Molecular Biology of the Institute of Hematology and Immunology includes 4 areas: molecular biology, hemoglobinopathies, enzymes and erythrocytic and cytogenetic membrane. Authors show relevant features of work developed in past years including researches of thalassemia allowing us to know the molecular bases of these hemoglobinopathies in our population. In the field of molecular biology initially we developed the Southern Blot technique; later, that of the polymerase chain reaction (PCR) and the inverse transcription, both very useful in the diagnosis and follow-up of patients presenting with malignant hemopathies. The presence of cytogenetic alterations (monosomies, trisomies, translocations, inversions and deletions) has been fully assessed in hematologic diseases and also with a great usefulness in the diagnosis, prognostic and follow-up of the patients. As regards the enzymopathies, it was possible to know the molecular alteration of variants lacking in glucose-6-phosphate dehydrogenase and with the development of the techniques for the study of proteins of erythrocytic membrane it was possible to determine the biochemical alteration of patients presenting with hereditary spherocytosis (HS).
Resultados relevantes en biología molecular, enzimopatías y citogenética en el período 1996-2010
Amor-Vigil,Ana María; Martínez-Antu?a,Gisela; Lavaut-Sánchez,Kalia; Pérez-Diez de los Ríos,Graciela; Hernández-Martínez,Ana; Estrada-del Cueto,Marianela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: the department of cellular and molecular biology of the institute of hematology and immunology includes 4 areas: molecular biology, hemoglobinopathies, enzymes and erythrocytic and cytogenetic membrane. authors show relevant features of work developed in past years including researches of thalassemia allowing us to know the molecular bases of these hemoglobinopathies in our population. in the field of molecular biology initially we developed the southern blot technique; later, that of the polymerase chain reaction (pcr) and the inverse transcription, both very useful in the diagnosis and follow-up of patients presenting with malignant hemopathies. the presence of cytogenetic alterations (monosomies, trisomies, translocations, inversions and deletions) has been fully assessed in hematologic diseases and also with a great usefulness in the diagnosis, prognostic and follow-up of the patients. as regards the enzymopathies, it was possible to know the molecular alteration of variants lacking in glucose-6-phosphate dehydrogenase and with the development of the techniques for the study of proteins of erythrocytic membrane it was possible to determine the biochemical alteration of patients presenting with hereditary spherocytosis (hs).
Algunas variables clínicas y de laboratorio en pacientes adultos con leucemia mieloide crónica tratados con interferón alfa recombinante + citosina arabinósido
Espinosa Martínez,Edgardo; Díaz Durán,Carmen Virginia; ávila Cabrera,Onel; Hernández Padrón,Carlos; Rodríguez,Luis Ramón; Izquierdo Cano,Lisset; Amor Vigil,Ana María; Lavaut Sánchez,Kalia; Espinosa Estrada,Edgardo; Wade Mateo,Maura; Estrada del Cueto,Marianela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2011,
Abstract: chronic myeloid leukemia is the most frequent myeloproliferative syndrome in adults. in a longitudinal retrospective study performed between january 1985 - december 2009, 46 patients in chronic phase diagnosed at the institute of hematology and immunology were evaluated. they received cytoreductor agent as first treatment followed by interferon ?2 + cytosar. forty one percent showed high risk sokal prognosis score. the most frequent clinical manifestations at diagnosis were asthenia (37 %), splenomegaly (31 %) and weigh lost (28.3 %). the partial and complete hematological response was of 26,8 % and 65.9 % after 6 months and the complete cytogenetic and molecular response was of 9.1 % and 16.3 %. the most frequent adverse reactions were: fever (34.9 %), thrombocytopenia (26.3 %) and general syndrome (23.8 %). resistance or intolerance to inf?2 was found in 47.8 % of the patients and 90.0 % died due to progression of the disease. the 5 year overall survival was of 63.8 % and the 3 years free event survival was of 68.9 %. according to sokal prognosis score the overall survival showed significant difference between groups (p= 0.005) but there was no significant difference for free event survival (p= 0.165). the inf?2 treatment in our patients showed better results than those obtained in different developed countries and is an effective therapeutic option in cuba.
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