oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

4 ( 1 )

2020 ( 2 )

2019 ( 243 )

2018 ( 318 )

Custom range...

Search Results: 1 - 10 of 234843 matches for " Ana Luisa; Díaz Torres "
All listed articles are free for downloading (OA Articles)
Page 1 /234843
Display every page Item
Definición de caso y clasificación de la infección por VIH y SIDA
Díaz Torres,Héctor Manuel; Lubián Caballero,Ana Luisa;
Revista Cubana de Medicina , 1998,
Abstract: the case definition as well as the systems of classification of the infection due to hiv and aids used up to date were reviewed. the limitations of their application in the clinical practice were also mentioned since the first systems were created to establish an epidemiological surveillance of the infection and because of the appareance of aids. the role of the definition and of the simplified classification systems that are utilized in countries with limitations of resources where it is not possible to use adequate diagnostic aids is also analyzed here. it was stressed that the utilization of theses systems in medical attention and research leads to the necessity of complementing clinical criteria with the use of efficient progression markers that help to estimate the prognosis and to evaluate the efficiency of the antiretroviral therapeutics. finally, it was assessed the impact of the alternate immunological and virological markers on the improvement of classification systems.
Definición de caso y clasificación de la infección por VIH y SIDA
Héctor Manuel Díaz Torres,Ana Luisa Lubián Caballero
Revista Cubana de Medicina , 1998,
Abstract: Se revisó la definición de caso y los sistemas de clasificación de la infección por VIH y SIDA empleados hasta la fecha y se mencionaron sus limitaciones para su aplicación en la práctica clínica porque los primeros sistemas fueron creados con fines de vigilancia epidemiológica de la infección y la aparición de casos de SIDA. Se comentó el papel de la definición y sistemas de clasificación simplificados que se usan en países con limitaciones de recursos, donde no es posible emplear medios de diagnóstico adecuados. Se hizo énfasis en que el uso de estos sistemas en la atención médica y la investigación implica la necesidad de complementar los criterios clínicos con el uso de marcadores de progresión eficientes, que ayuden a estimar el pronóstico y permitan evaluar la eficacia de la terapéutica antirretroviral. Finalmente se valoró el impacto de los marcadores sustitutivos inmunológicos y virológicos en el perfeccionamiento de los sistemas de clasificación. The case definition as well as the systems of classification of the infection due to HIV and AIDS used up to date were reviewed. The limitations of their application in the clinical practice were also mentioned since the first systems were created to establish an epidemiological surveillance of the infection and because of the appareance of AIDS. The role of the definition and of the simplified classification systems that are utilized in countries with limitations of resources where it is not possible to use adequate diagnostic aids is also analyzed here. It was stressed that the utilization of theses systems in medical attention and research leads to the necessity of complementing clinical criteria with the use of efficient progression markers that help to estimate the prognosis and to evaluate the efficiency of the antiretroviral therapeutics. Finally, it was assessed the impact of the alternate immunological and virological markers on the improvement of classification systems.
Vigilancia del personal de laboratorio expuesto al VIH y otros virus de transmisión sanguínea
Regalado Alfonso,Lázaro; Díaz Torres,Héctor; Lubián Caballero,Ana Luisa; Martín García,Regina Zonia;
Revista Cubana de Medicina Tropical , 2002,
Abstract: the results of 15 years of surveillance of the laboratory personnel with different levels of exposure to hiv and other bloodborne viruses like hcv, hbv and htlv-1 were presented. various biosafety measures were applied and elisa was used as the main lab technique. 628 serum samples were analyzed for hiv whereas 354 for the rest of the viruses but no lab-acquired infection was detected. this indicates the low incidence of hiv infection in health care workers at risk and the great importance of biosafety measures in the prevention and early detection of these infections.
Empleo del AMPLICOR HIV-1 DNA test v 1.5 en el diagnóstico de la infección perinatal por el VIH-1 en Cuba
Machado Zaldívar,Liuber Y.; Blanco de Armas,Madeline; Lubián Caballero,Ana Luisa; Díaz Torres,Héctor M.;
Revista Cubana de Medicina Tropical , 2010,
Abstract: introduction: hiv-1 proviral dna amplification is the preferential method for hiv diagnosis infection in infants and it has been used in cuba since 1992. amplicor hiv-1 dna kit is an in vitro qualitative assay for the detection of hiv-1 proviral dna in the whole blood. objective: this paper showed the results of the use of this kit for the first time in cuba for the perinatal diagnosis of hiv-1 infection. methods: three hundred forty six whole blood samples from children of hiv seropositive women were analyzed by the amplicor hiv-1 dna kit in the period 2005-2007. results: among the tested samples, six were positive, and 340 negative. conclusions: the assay was reproducible under the cuban conditions and the achieved results made the diagnosis and follow up of children of hiv-1 seropositive mothers possible.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Ana Luisa Lubián Caballero,Héctor Manuel Díaz Torres,Eladio Silva Cabrera,María Teresa Pérez Guevara
Revista Cubana de Medicina , 1998,
Abstract: Se estudiaron, en el período comprendido desde 1991 hasta 1996, 26 352 muestras de suero procedentes de diferentes grupos de riesgo y donantes de sangre para conocer la seroprevalencia de anticuerpos contra el virus linfotrópico de las células T humanas tipo I (HTLV-I) y como continuación de investigaciones realizadas entre 1989 y 1990. Se empleó el sistema de ELISA DAVIH-HTLV-I para el pesquisaje de anticuerpos y como prueba confirmatoria, el Western Blot DAVIH-Blot HTLV-I, ambos de Laboratorios DAVIH, Cuba. Se confirmó la presencia de anticuerpos anti HTLV-I en 10 personas y en la mayoría de ellas el estudio epidemiológico logró esclarecer la vía probable de contagio. El índice de seropositividad observado fue de 0,037 %, lo que en comparación con las tasas de seroprevalencia reportadas para el área del Caribe resulta bajo. In the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human T-lymphotropic virus type I (HTLV-I), and also as a continuation of researches carried out between 1989 and 1990. The ELISA DAVIH-HTLV-I system was used for the antibodies investigation, and as a certifying test, the Western Blot DAVIH-Blot HTLV-I was used too, both from the Cuban DAVIH Laboratories. The presence of anti HTLV-I antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. The seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the Caribbean area.
Empleo del AMPLICOR HIV-1 DNA test v 1.5 en el diagnóstico de la infección perinatal por el VIH-1 en Cuba Use of Amplicor HIV-1 DNA test v.1.5 in diagnosis of perinatal infection from HIV-1 in Cuba
Liuber Y. Machado Zaldívar,Madeline Blanco de Armas,Ana Luisa Lubián Caballero,Héctor M. Díaz Torres
Revista Cubana de Medicina Tropical , 2010,
Abstract: INTRODUCCIóN: la amplificación del ADN proviral del VIH-1, constituye un método preferencial para el diagnóstico perinatal, empleado en Cuba desde 1992. El estuche AMPLICOR HIV-1 DNA es un ensayo cualitativo in vitro para la detección del ADN proviral de VIH-1 en sangre total. OBJETIVO: en el presente trabajo se reportan los resultados del empleo de este estuche por primera vez en Cuba para el diagnóstico perinatal de VIH-1. MéTODOS: entre 2005 y 2007 se trabajaron mediante el estuche AMPLICOR HIV-1 DNA, 346 muestras de sangre total de ni os nacidos de madres seropositivas al VIH-1. RESULTADOS: del total de muestras trabajadas, 6 resultaron positivas y 340 fueron negativas. CONCLUSIONES: el estuche fue reproducible en las condiciones cubanas y los resultados obtenidos permitieron realizar el diagnóstico y seguimiento de ni os nacidos de madres seropositivas. INTRODUCTION: HIV-1 proviral DNA amplification is the preferential method for HIV diagnosis infection in infants and it has been used in Cuba since 1992. AMPLICOR HIV-1 DNA kit is an in vitro qualitative assay for the detection of HIV-1 proviral DNA in the whole blood. OBJECTIVE: this paper showed the results of the use of this kit for the first time in Cuba for the perinatal diagnosis of HIV-1 infection. METHODS: three hundred forty six whole blood samples from children of HIV seropositive women were analyzed by the AMPLICOR HIV-1 DNA kit in the period 2005-2007. RESULTS: among the tested samples, six were positive, and 340 negative. CONCLUSIONS: the assay was reproducible under the Cuban conditions and the achieved results made the diagnosis and follow up of children of HIV-1 seropositive mothers possible.
Diagnóstico serológico del VIH-1 en muestras de sangre seca en papel de filtro por el sistema DAVIH Dot VIH-1
René Grana Sánchez,María Teresa Pérez Guevara,Ana Luisa Lubián Caballero,Héctor Díaz Torres
Revista Cubana de Medicina Tropical , 1998,
Abstract: Se evaluaron muestras de sangre seca en papel de filtro por el sistema DAVIH Dot VIH-1 de Laboratorios DAVIH (La Habana, Cuba). Las muestras utilizadas, 103 positivas y 105 negativas, estaban confirmadas de forma previa por DAVIH Blot de Laboratorios DAVIH. Para utilizar la sangre seca en papel de filtro, se realizaron modificaciones al procedimiento original en cuanto a los tiempos de incubación de las muestras y conjugado, y la agitación en ambas etapas. Se obtuvieron resultados de sensibilidad y especificidad relativas de 100 y 99,05 %, respectivamente. Dried blood specimens spotted on filter paper were evaluated by the HIV-1 DAVIH Dot system from DAVIH Laboratories (Havana, Cuba). The samples used, 103 positive and 105 negative, were previously confirmed by DAVIH Blot from DAVIH Laboratories. In order to use the dried blood on filter paper some modifications were made to the original procedure regarding the times of incubation of the samples and conjugate, and the agitation in both stages. Relative sensitivity and specificity of 100 and 99.05 %, respectively, were attained.
Vigilancia del personal de laboratorio expuesto al VIH y otros virus de transmisión sanguínea
Lázaro Regalado Alfonso,Héctor Díaz Torres,Ana Luisa Lubián Caballero,Regina Zonia Martín García
Revista Cubana de Medicina Tropical , 2002,
Abstract: Se reportaron los resultados de 15 a os de vigilancia del personal de laboratorio con diferentes grados de exposición al virus de inmunodeficiencia humana (VIH) y otros virus de transmisión sanguínea como el virus de hepatitis C, virus de hepatitis B y el virus linfotrópico de células T humanas (HTLV-I). Se emplearon diferentes medidas de bioseguridad y como técnica de laboratorio fundamentalmente el ELISA. De 628 muestras analizadas para VIH y 354 para el resto de los virus no se encontraron infecciones adquiridas del laboratorio, lo que confirma la baja incidencia de la infección por VIH en trabajadores de la salud expuestos y el gran valor de las medidas de bioseguridad en la prevención y detección temprana de estas infecciones. The results of 15 years of surveillance of the laboratory personnel with different levels of exposure to HIV and other bloodborne viruses like HCV, HBV and HTLV-1 were presented. Various biosafety measures were applied and ELISA was used as the main lab technique. 628 serum samples were analyzed for HIV whereas 354 for the rest of the viruses but no lab-acquired infection was detected. This indicates the low incidence of HIV infection in health care workers at risk and the great importance of biosafety measures in the prevention and early detection of these infections.
Análisis de los polimorfismos G199A, Ncol del gen ANK1 y Memphis I del gen SLC4A1 en Individuos sanos y pacientes mexicanos con esferocitosis hereditaria
Camacho-Torres, Ana Luisa;Sánchez-López, Josefina Yoaly;Mesa-Cornejo, Viviana Matilde;Ibarra, Bertha;Javier Perea-Díaz, Francisco;
Gaceta médica de México , 2006,
Abstract: background. in mexico, hereditary spherocytosis (hs) is the main cause of hereditary hemolytic anemia, due to mutations of one or more genes involved in the erythrocyte membrane, making it difficult to identify the primary gene. objective. with the purpose of estimating the use of the polymorphisms g199a and ncol of ankl gene, and memphis i of slc4a1 gene, as genetic markers to screen this disease, we searched the allelic and genotypic frequencies in 45 dna samples of hs patients and 28 from healthy individuals. results. allelic and genotypic frequencies were similar in both studied groups for the g199a and memphis i polymorphisms, with low frequency of heterozygosis showing its limited use as a genetic marker. the allelic and genotypic frequencies of the ncol polymorphism were also similar in both groups, however a higher heterozygote frequency was observed (0.49 and 0.43 in patients and healthy individuals), a feature that may turn it into a useful genetic marker. conclusions. since there are other genes implicated in the molecular pathology of the hs, we consider it necessary to continue analyzing other polymorphisms of the genes involved in hereditary spherocytosis among the mexican population,
Infección por el virus de la inmunodeficiencia humana tipo2: Presentación de 1 caso
González del Valle González,Zulema; Díaz Torres,Héctor Manuel; Vázquez Vigoa,Alfredo; Lubián Caballero,Ana Luisa; álvarez García,Alejandro;
Revista Cubana de Medicina , 2003,
Abstract: the case of a 69 year-old patient from the municipality of centro habana that was admitted at "hermanos ameijeiras" hospital for presenting a clinical picture of fever, upper diarrheas and weight loss, is reported. the presence of lesions suggestive of oral candidiasis was proved on the physical examination. accelerated erythrosedimentation, increase of lactic acid dehydrogenase and severe reduction of the total count of lymphocytes cd4+ were found in the complementary studies. the presence of antibodies against the hiv-2 was confirmed by serological study in 2 samples of blood taken on different dates. this case corresponds to the tenth diagnosis of hiv-2 infection made in cuba, and it is of interest that in spite of the long period of incubation, it was made at an advanced stage of the infection due to the clinical suspicion of immunodeficiency and not as a result of previous screening studies.
Page 1 /234843
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.