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Search Results: 1 - 10 of 53354 matches for " Ana Laura Ibá?ez "
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Aspectos reproductivos de una población del charal Menidia jordani (Woolman) del Lago de Metztitlán, Hidalgo
Ibá?ez, Ana Laura;García Calderón, José Luis;Torres-Orozco B., Roberto;
Hidrobiológica , 2008,
Abstract: menidia jordani is a silverside species naturally distributed in the mexican and lerma drainage areas and has in metztitlán lake it most eastern area of distribution in a lentic system. the analysis of the reproductive biology of this species showed that even metztitlán lake is the extreme frontier of their distribution conserved its reproduction phenology in relation with other populations. the highest standard length was 58 mm and the length at first sexual maturation was at 43 mm. sexual ratio inequality was manifested in mayor proportion of females during all collects with exception of january of 2004.
Aspectos reproductivos de una población del charal Menidia jordani (Woolman) del Lago de Metztitlán, Hidalgo Reproductive features of the "charal" Menidia jordani (Woolman) from Lake of Metztitlán, Hidalgo
Ana Laura Ibá?ez,José Luis García Calderón,Roberto Torres-Orozco B.
Hidrobiológica , 2008,
Abstract: Menidia jordani es una especie de charal que se distribuye de manera natural en las cuencas de México y del Río Lerma, y tiene en el Lago de Metztitlán, Hidalgo, el límite oriental de su área de distribución en ambientes lénticos. Los resultados del análisis de algunos aspectos de la biología reproductiva de la población de M. jordani que habita en el Lago de Metztitlán mostraron que, a pesar de vivir en un ambiente ubicado en la frontera de su área de distribución, conserva una fenología reproductiva similar a la de otras poblaciones. La máxima longitud estándar observada fue de 58 mm y la talla a la primera madurez sexual fue de 43 mm. Una notable desigualdad en la proporción de sexos a favor de las hembras se observó en todas las colectas, con excepción de la de enero del 2004. Menidia jordani is a silverside species naturally distributed in the Mexican and Lerma drainage areas and has in Metztitlán Lake it most eastern area of distribution in a lentic system. The analysis of the reproductive biology of this species showed that even Metztitlán Lake is the extreme frontier of their distribution conserved its reproduction phenology in relation with other populations. The highest standard length was 58 mm and the length at first sexual maturation was at 43 mm. Sexual ratio inequality was manifested in mayor proportion of females during all collects with exception of January of 2004.
Assessing the Performance of Two Hydrologic Models for Forecasting Daily Streamflows in the Cazones River Basin (Mexico)  [PDF]
Fernando González-Leiva, Rodrigo Valdés-Pineda, Juan B. Valdés, Laura A. Ibá?ez-Castillo
Open Journal of Modern Hydrology (OJMH) , 2016, DOI: 10.4236/ojmh.2016.63014
Abstract: Floods have caused significant human and economic losses in the Cazones River Basin, located on the Gulf of Mexico. Despite this knowledge, steps towards the design and implementation of an early warning system for the Cazones are still a pending task. In this study we contributed by establishing a hydrological scheme for forecasting mean daily discharges in the Cazones Basin. For these purposes, we calibrated, validated and compared the HyMod model (HM) which is physics-based, and an autoregressive-based model coupled with the Discrete Kalman Filter (ARX-DKF). The ability of both models to accurately predict discharges proved satisfactory results during the validation period with RMSEHYMOD = 2.77 [mm/day]; and RMSEARX-DKF = [2.38 mm/day]. Further analysis based on a Streamflow Assimilation Ratio (SAR) revealed that both models underestimate the discharges in a similar proportion. This evaluation also showed that, under the most common conditions, the simpler stochastic model (ARX-DKF) performs better; however, under extreme hydrological conditions the deterministic HM model reveals a better performance. These results are discussed under the context of future applications and additional requirements needed to implement an early warning hydrologic system for the Cazones Basin.
Utilidad de la ferritina sérica para evaluar depósitos de hierro maternos en el post parto inmediato Usefulness of serum ferritin to assess maternal iron stores in the early postpartum period
Silvia Haydée Langini,Silvana Fleischman,Laura Beatriz López,Laura Moratal Ibá?ez
Acta bioqu?-mica cl?-nica latinoamericana , 2004,
Abstract: La ferritina sérica (FS), indicadora de depósitos de hierro (Fe), es cuestionable en el puerperio, mientras que el receptor soluble de transferrina (RsT) parecería más confiable. Por ello, se estudió la FS en las 24 h post parto en 88 mujeres atendidas en el Hospital Diego Paroissien. Se determinó: Hematocrito (Hto), Hemoglobina (Hb), recuento de glóbulos rojos (GR) y blancos (GB); en suero: RsT, FS y Proteína C-Reactiva. Se analizó sensibilidad y especificidad de FS mediante el modelo del operador-receptor (ROC), considerando al RsT como "estándar de oro". Los valores (media ± DE y rangos) fueron: Hto (%) 35 ± 5 (22 - 47); Hb (g/L) 112 ± 17 (61 - 150); GR x 103/mm3 3.843 ± 530 (2.500 - 5.300); GB/mm3 9.644 ± 2.599 (5.300 - 21.000); RsT (mg/L) 4,93 ± 2,93 (0,54 - 14,77); FS (μg/L) 33 ± 39 (0 - 210); PCR (+) en 92,4% de los casos. FS evidenció un área bajo la curva de 0,795 con elevada sensibilidad (83%) y especificidad de 63% para un punto de corte de 25 μg/L. Además, FS correlacionó con RsT (r = -0,436) y con el cociente RsT/FS (r = -0,919). Estos resultados sugieren que FS sería de utilidad en el post parto inmediato, siendo aconsejable utilizar el punto de corte de 25 μg/L. Serum ferritin (SF) may not be a reliable indicator of iron (Fe) stores during the puerperium. Therefore, it was compared to the soluble transferrin receptor (sTfR) in 24 h post partum women (n = 88), assisted at Diego Paroissien Hospital (Buenos Aires, Argentina). Hematocrit (Hct), hemoglobin (Hb), red blood cells (RBC) and white blood cells (WBC) were determined in fasting blood samples using an electronic counter (Mega); SF by ELISA (IMx Ferritina, Abbott); sTfR by ELISA (Orion Diagnostica) and C-Reactive Protein (PCR-Latex, Wiener lab). Statistical analysis (Receiver Operating Characteristics, ROC) were performed using the sTfR as "gold standard". Results: (mean ± SD) (range): Hct (%) 35 + 5 (22 - 47); Hb (g/L) 112 ± 17 (61 - 150); RBC x 103/mm3 3843 ± 530 (2500-5300); WBC/mm3 9644±2599 (5300 -21000); sTfR (mg/L) 4.93 ± 2.93 (0.54 - 14.77); SF(μg/L) 33 ± 39 (0 - 210); PCR (+) in 92.4%. Sensitivity and specificity of SF were, respectively: 83% and 63% for a cut-off point of 25 μg /L (area under ROC plot 0.795, 95% reference interval: 0.694 - 0.897). SF correlated inversely with sTfR (r = -0.436) and with the sTfR/SF ratio (r = -0.919). These results suggest that SF may be a useful indicator of maternal Fe stores during the early perinatal period, although its cost may limit rutinary laboratory use.
A Single Amino Acid Change Converts the Sugar Sensor SGLT3 into a Sugar Transporter
Laura Bianchi,Anaez-Sampedro
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0010241
Abstract: Sodium-glucose cotransporter proteins (SGLT) belong to the SLC5A family, characterized by the cotransport of Na+ with solute. SGLT1 is responsible for intestinal glucose absorption. Until recently the only role described for SGLT proteins was to transport sugar with Na+. However, human SGLT3 (hSGLT3) does not transport sugar but causes depolarization of the plasma membrane when expressed in Xenopus oocytes. For this reason SGLT3 was suggested to be a sugar sensor rather than a transporter. Despite 70% amino acid identity between hSGLT3 and hSGLT1, their sugar transport, apparent sugar affinities, and sugar specificity differ greatly. Residue 457 is important for the function of SGLT1 and mutation at this position in hSGLT1 causes glucose-galactose malabsorption. Moreover, the crystal structure of vibrio SGLT reveals that the residue corresponding to 457 interacts directly with the sugar molecule. We thus wondered if this residue could account for some of the functional differences between SGLT1 and SGLT3.
Impact of Cadmium Exposure during Pregnancy on Hepatic Glucocorticoid Receptor Methylation and Expression in Rat Fetus
Paula Castillo, Freddy Ibá?ez, Angélica Guajardo, Miguel N. Llanos, Ana M. Ronco
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044139
Abstract: Adverse fetal environment due to maternal undernutrition or exposure to environmental chemicals alters glucocorticoid (GC) metabolism increasing the risk of metabolic disorders in adulthood. In this study, we investigated the effects of maternal exposure to cadmium (Cd, 50 ppm) during pregnancy in the methylation of fetal hepatic glucocorticoid receptor promoter (GR) and the correlation with its expression and that of the DNA methyltransferases (DNMT1a and 3a). We also studied the expression of liver phosphoenolpyruvate carboxykinase (PEPCK) and acyl-CoA oxidase (AOX), two enzymes involved in the metabolism of carbohydrates and lipids respectively. The methylation of the rat GR gene exon 110 (GR110) in nucleotides -2536 to -2361 was analyzed by pyrosequencing. Quantitative real time PCR was used to assess hepatic GR, PEPCK and AOX mRNA, and their protein levels using Western blotting analysis. Differential methylation was noted across groups at all CpG sites in the GR exon 110 in a sex-dependent manner. In males, CpG were more methylated than the controls (185±21%, p<0.001) but only CpG sites 1,6,7 and 9 showed a significantly different extent of methylation. In addition, a lower expression of GR (mRNA and protein) was found. On the contrary, in females, CpG were less methylated than the controls (62±11%, p<0.05) and overexpressed, affecting PEPCK and AOX expression, which did not change in males. The GR methylation profile correlates with DNMT3a expression which may explain epigenetic sex-dependent changes on GR110 promoter induced by Cd treatment. In conclusion, Cd exposure during pregnancy affects fetal liver DNMT3a resulting in sex-dependent changes in methylation and expression of GR110. Although these effects do not seem to be directly involved in the low birth weight and height, they may have relevant implications for long-term health.
High throughput SNP discovery and genotyping in grapevine (Vitis vinifera L.) by combining a re-sequencing approach and SNPlex technology
Diego Lijavetzky, José Cabezas, Ana Ibá?ez, Virginia Rodríguez, José M Martínez-Zapater
BMC Genomics , 2007, DOI: 10.1186/1471-2164-8-424
Abstract: In order to develop a large SNP set of wide application in grapevine we followed a systematic re-sequencing approach in a group of 11 grape genotypes corresponding to ancient unrelated cultivars as well as wild plants. Using this approach, we have sequenced 230 gene fragments, what represents the analysis of over 1 Mb of grape DNA sequence. This analysis has allowed the discovery of 1573 SNPs with an average of one SNP every 64 bp (one SNP every 47 bp in non-coding regions and every 69 bp in coding regions). Nucleotide diversity in grape (π = 0.0051) was found to be similar to values observed in highly polymorphic plant species such as maize. The average number of haplotypes per gene sequence was estimated as six, with three haplotypes representing over 83% of the analyzed sequences. Short-range linkage disequilibrium (LD) studies within the analyzed sequences indicate the existence of a rapid decay of LD within the selected grapevine genotypes. To validate the use of the detected polymorphisms in genetic mapping, cultivar identification and genetic diversity studies we have used the SNPlex? genotyping technology in a sample of grapevine genotypes and segregating progenies.These results provide accurate values for nucleotide diversity in coding sequences and a first estimate of short-range LD in grapevine. Using SNPlex? genotyping we have shown the application of a set of discovered SNPs as molecular markers for cultivar identification, linkage mapping and genetic diversity studies. Thus, the combination a highly efficient re-sequencing approach and the SNPlex? high throughput genotyping technology provide a powerful tool for grapevine genetic analysis.Single nucleotide polymorphisms (SNP) and insertions/deletions (INDELs) are the most abundant type of DNA sequence polymorphisms and can be theoretically found within every genomic sequence [1,2]. They can be used as genetic markers for many genetic applications such as cultivar identification, construction of genetic
First record of parasites of Dosidicus gigas (d' Orbigny, 1835) (Cephalopoda: Ommastrephidae) from the Humboldt Current system off Chile
Pardo-Gandarillas,M. Cecilia; Lohrmann,Karin B; Valdivia,Ana L; Ibá?ez,Christian M;
Revista de biología marina y oceanografía , 2009, DOI: 10.4067/S0718-19572009000200013
Abstract: the parasitic fauna of 124 jumbo squids (dosidicus gigas) caught from oceanic and coastal waters off central chile (32os-40os) was described and quantified. the 97% of the squids harboured at least one parasitic taxon. prevalence of infection and abundance were established for each parasite species. each parasitic taxon was described based on light microscopy and scanning electron microscopy images. the parasitic fauna was composed of larval cestodes: hepatoxylon trichiuri (abundance: 2.6; prevalence: 70.2%), tentacularia coryphaenae (abundance: 0.1; prevalence: 5.6%), a tetraphyllidean plerocercoid (abundance: 9.1; prevalence: 83.1%), pelichnibothrium speciosum and the nematodes anisakis type i (abundance: 0.06; prevalence: 6.5%) and anisakis type ii (abundance: 0.52; prevalence: 17.7%). around nine taxa have been reported in d. gigas smaller than 40 cm mantle length, five of them were found in this study (tetraphyllidean plerocercoid, tentacularia coryphaenae, pelichnibothrium speciosum, and anisakis type i and ii), however, in bigger squids. the parasite h. trichiuri is a new record for d. gigas in these latitudes of the south pacific, comprising the southern limit of its distribution range. the tetraphyllidea plerocercoid could correspond to phyllobothrium based on the shape and tissues of its apical organ, which is very similar to the accesory suckers. the nematodes anisakis type i and ii can only be distinguished by the tail shape and presence-absence of a mucron. it is suggested to carry out molecular analysis for diagnosis of larval stages when morphological characters are lacking.
Vértigo y ficción, una historia contada con imágenes. Noticieros de televisión en Colombia 1954-1970
Carrillo Hernández,Adriana María; Monta?a Ibá?ez,Ana María;
Signo y Pensamiento , 2006,
Abstract: this document compiles the history of tv news programs in colombia from two main perspectives: on the one hand, the newsreel as a legacy of the cinema and other media, in terms of technology, format, and staff; on the other, the actual effect of new technologies in the work routines and changes suffered by the 'news' concept. we intend to show how the first stages of colombian television were mainly an experimental activity whereby the tv journalist's profession was developed by the empirical performing of tasks, since the first journalism and communication schools only came into being in the 60's. we conclude that technology plays an essential role in the way events are narrated, and in how the 'news' concept is conceived in each of the historical moments dealt with herein.
Síndrome de Currarino y enfermedad de Hirschsprung: Una asociación poco frecuente Currarino′s syndrome and Hirschsprung′s disease: A rare combination
David Ibá?ez M,Ana Vela M,Beatriz Izquierdo H,Carolina Muel G
Revista Chilena de Radiología , 2012,
Abstract: El Síndrome de Currarino consiste en agenesia sacra parcial de herencia genética autosómica dominante parcial, que afecta las vértebras sacras S2 a S5, preservándose S1. En la forma más severa del síndrome, a la agenesia sacra se asocian masa presacra y malformaciones anorrectales y urogenitales. La enfermedad de Hirschsprung es una malformación congénita del intestino grueso debida a la ausencia de células ganglionares intrínsecas parasimpáticas de la submucosa y de los plexos mientéricos. Presentamos el caso de un ni o de 3 a os con Síndrome de Currarino asociado a enfermedad de Hirschsprung. The Currarino syndrome is an autosomal dominant partial sacral agenesis involving sacral vertebrae S2 to S5, with presevation of the S1 vertebrae. In the most severe form of the syndrome, the sacral agenesis is associated to presacral mass, anorrectal and urogenital malformations. Hirschsprung′s disease is a congenital malformation of the hindgut determined by the absence if parasympathetic intrinsic ganglion cells in the submucosal and myenteric plexuses. We present a case of a 3 years old male child affected from Currarino syndrome associated to Hirschprung′s disease.
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