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Search Results: 1 - 10 of 233782 matches for " Ana Caroline P. Gandara "
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Blood Meal-Derived Heme Decreases ROS Levels in the Midgut of Aedes aegypti and Allows Proliferation of Intestinal Microbiota
Jose Henrique M. Oliveira,Renata L. S. Gon?alves,Flavio A. Lara,Felipe A. Dias,Ana Caroline P. Gandara,Rubem F. S. Menna-Barreto,Meredith C. Edwards,Francisco R. M. Laurindo,Mário A. C. Silva-Neto,Marcos H. F. Sorgine,Pedro L. Oliveira
PLOS Pathogens , 2011, DOI: 10.1371/journal.ppat.1001320
Abstract: The presence of bacteria in the midgut of mosquitoes antagonizes infectious agents, such as Dengue and Plasmodium, acting as a negative factor in the vectorial competence of the mosquito. Therefore, knowledge of the molecular mechanisms involved in the control of midgut microbiota could help in the development of new tools to reduce transmission. We hypothesized that toxic reactive oxygen species (ROS) generated by epithelial cells control bacterial growth in the midgut of Aedes aegypti, the vector of Yellow fever and Dengue viruses. We show that ROS are continuously present in the midgut of sugar-fed (SF) mosquitoes and a blood-meal immediately decreased ROS through a mechanism involving heme-mediated activation of PKC. This event occurred in parallel with an expansion of gut bacteria. Treatment of sugar-fed mosquitoes with increased concentrations of heme led to a dose dependent decrease in ROS levels and a consequent increase in midgut endogenous bacteria. In addition, gene silencing of dual oxidase (Duox) reduced ROS levels and also increased gut flora. Using a model of bacterial oral infection in the gut, we show that the absence of ROS resulted in decreased mosquito resistance to infection, increased midgut epithelial damage, transcriptional modulation of immune-related genes and mortality. As heme is a pro-oxidant molecule released in large amounts upon hemoglobin degradation, oxidative killing of bacteria in the gut would represent a burden to the insect, thereby creating an extra oxidative challenge to the mosquito. We propose that a controlled decrease in ROS levels in the midgut of Aedes aegypti is an adaptation to compensate for the ingestion of heme.
An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus
José M. C. Ribeiro ,Fernando A. Genta,Marcos H. F. Sorgine,Raquel Logullo,Rafael D. Mesquita,Gabriela O. Paiva-Silva,David Majerowicz,Marcelo Medeiros,Leonardo Koerich,Walter R. Terra,Clélia Ferreira,André C. Pimentel,Paulo M. Bisch,Daniel C. Leite,Michelle M. P. Diniz,Jo?o Lídio da S. G. V. Junior,Manuela L. Da Silva,Ricardo N. Araujo,Ana Caroline P. Gandara,Sébastien Brosson,Didier Salmon,Sabrina Bousbata,Natalia González-Caballero,Ariel Mariano Silber,Michele Alves-Bezerra,Katia C. Gondim,Mário Alberto C. Silva-Neto,Georgia C. Atella,Helena Araujo,Felipe A. Dias,Carla Polycarpo,Raquel J. Vionette-Amaral,Patrícia Fampa,Ana Claudia A. Melo,Aparecida S. Tanaka,Carsten Balczun,José Henrique M. Oliveira,Renata L. S. Gon?alves,Cristiano Lazoski,Rolando Rivera-Pomar,Luis Diambra,Günter A. Schaub,Elói S. Garcia,Patrícia Azambuja,Glória R. C. Braz ,Pedro L. Oliveira
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002594
Abstract: The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas' disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as pro-enzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet.
Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle
Felipe de Almeida Dias?,Barbara Guerra?,Larissa Rezende Vieira?,Hugo Diego Perdomo?,Ana Caroline Paiva Gandara,Raquel Juliana Vionette do Amaral?,Renata Estebanez Vollú?,Suzete Araujo Oliveira Gomes?,Flavio Alves Lara?,Marcos Henrique Ferreira Sorgine
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0004186
Abstract: Background Here we report the monitoring of the digestive tract colonization of Rhodnius prolixus by Trypanosoma cruzi using an accurate determination of the parasite load by qPCR coupled with fluorescence and bioluminescence imaging (BLI). These complementary methods revealed critical steps necessary for the parasite population to colonize the insect gut and establish vector infection. Methodology/Principal Findings qPCR analysis of the parasite load in the insect gut showed several limitations due mainly to the presence of digestive-derived products that are thought to degrade DNA and inhibit further the PCR reaction. We developed a real-time PCR strategy targeting the T. cruzi repetitive satellite DNA sequence using as internal standard for normalization, an exogenous heterologous DNA spiked into insect samples extract, to precisely quantify the parasite load in each segment of the insect gut (anterior midgut, AM, posterior midgut, PM, and hindgut, H). Using combined fluorescence microscopy and BLI imaging as well as qPCR analysis, we showed that during their journey through the insect digestive tract, most of the parasites are lysed in the AM during the first 24 hours independently of the gut microbiota. During this short period, live parasites move through the PM to establish the onset of infection. At days 3–4 post-infection (p.i.), the parasite population begins to colonize the H to reach a climax at day 7 p.i., which is maintained during the next two weeks. Remarkably, the fluctuation of the parasite number in H remains relatively stable over the two weeks after refeeding, while the populations residing in the AM and PM increases slightly and probably constitutes the reservoirs of dividing epimastigotes. Conclusions/Significance These data show that a tuned dynamic control of the population operates in the insect gut to maintain an equilibrium between non-dividing infective trypomastigote forms and dividing epimastigote forms of the parasite, which is crucial for vector competence.
Relationship between Symptoms, Use of PPE and Habits Related to Occupational Exposure to BTEX Compounds in Workers of Gas Stations in Bahia, Brazil  [PDF]
Lidmary P. Alves, Daniela S. P. Vieira, Luís Sérgio S. Nunes, Lícia P. S. Cruz, Ana Caroline S. Reis, ícaro V. S. Gomes, Sameque R. Luz, Akácia V. S. Santos, Mabel B. Esteves
Journal of Environmental Protection (JEP) , 2017, DOI: 10.4236/jep.2017.85042
Abstract: To show the risks and harm caused by the occupational exposure to the benzene, toluene, ethylbenzene and xylenes compounds (BTEX), the working conditions of 113 persons employed at gas stations in the cities of Riach?o do Jacuipe, Feira de Santana and Salvador in the state of Bahia, Brazil were investigated by experts who correlated protection measures required by law and those applied to the workers. Data collection was performed by help of a questionnaire and non-participant observation record. The age groups of the workers in the three cities surveyed were between 18 to 25 years (30.08%) and 26 to 33 years (34.51%) of the total. The study revealed an association between occupational habits of workers at gas stations and the prevalence of symptoms due to the exposure to the BTEX compounds. Regarding the use of Personal Protective Equipment (PPE) most workers wear boots. The data show that these workers reported symptoms such as headache, rhinitis, leg pain, drowsiness, cough, fatigue, insomnia, tremors and palpitations. These disorders are related back to previous exposure to BTEX compounds which is associated with inadequate occupational habits of the workers which potentiate chemical contamination in their respective environment.
Reda??o científica e a qualidade dos artigos: em busca de maior impacto
Cáceres, Ana Manhani;Gandara, Juliana Perina;Puglisi, Marina Leite;
Jornal da Sociedade Brasileira de Fonoaudiologia , 2011, DOI: 10.1590/S2179-64912011000400019
Abstract: given the latent concern of scientists and editors on the quality of scientific writing, the aim of this paper was to present topics on the recommended structure of peer-reviewed papers. we described the key points of common sections of original papers and proposed two additional materials that may be useful for scientific writing: one particular guide to help the organization of the main ideas of the paper; and a table with examples of non desirable and desirable structures in scientific writing.
Synthesis of the C1-C9 fragment of the potent antitumor agent dictyostatin
Dias, Luiz C.;Sant'Ana, Danilo P.;Vieira, Ygor W.;Gon?alves, Caroline C. S.;Lima, Dimas J. P.;
Journal of the Brazilian Chemical Society , 2012, DOI: 10.1590/S0103-50532012000200022
Abstract: we describe herein a short and efficient synthesis of the c1-c9 fragment of the potent antitumor agent dictyostatin. notable features of this approach include a sharpless asymmetric epoxidation followed by epoxide opening under myashita's conditions to introduce the stereogenic centers at c6 and c7, and a horner-wadsworth-emmons type reaction under ando's conditions to construct the z-double bond of the 1,3-(z,e)-diene system.
Significados do feminino no discurso de alcoolistas e a interface com a saúde mental
Lima, Helder de Pádua;Braga, Violante Augusta Batista;Carvalho, Luciana Vieira de;Morais, Ana Caroline de Oliveira;
Texto & Contexto - Enfermagem , 2010, DOI: 10.1590/S0104-07072010000300011
Abstract: given the impact caused by alcohol to individual and collective health, we reflect that in the society to which we belong, a series of repercussions involving the female gender with regard to mental health may be revealed. the main objective in this study is to capture from the speech of alcoholics the meanings of the feminine and their interface with mental health. this is a descriptive study with a qualitative approach, involving 20 alcoholics anonymous participants from fortaleza, ceará, brazil. the data was collected through individual interviews using semi-structured questions and analyzed according to the content analysis method. from such research, the following meanings emerged: social support; suffering and illness; passiveness; and submission to the male. the study concludes that perceived senses constitute relevant factors in the patterns of health risks. it is also noted that social processes related to the female gender cause repercussions in mental health.
Helder de Pádua Lima,Violante Augusta Batista Braga,Luciana Vieira de Carvalho,Ana Caroline de Oliveira Morais
Texto & Contexto - Enfermagem , 2010,
Abstract: Al tener en cuenta el impacto causado por el alcoholismo para la salud individual y colectiva, se puede reflexionar que en la sociedad a la cual pertenecemos, una serie de efectos relacionados con la mujer pueden ser desencadenados, incluso en lo que se refiere a la salud mental. El objetivo principal de este estudio es captar en el discurso de los alcohólicos los significados de lo femenino y su interfaz con la salud mental. Se trata de un estudio descriptivo con enfoque cualitativo, realizado con 20 participantes de un grupo de Alcohólicos Anónimos en la ciudad de Fortaleza, Ceará, Brazil. Las informaciones se recolectaron a través de entrevistas individuales, con preguntas semi-estructuradas, y se analizaron según el método del análisis de contenido. De la investigación surgieron los siguientes significados: apoyo social; sufrimiento y enfermedad; pasividad y sumisión a lo masculino. Se concluye que los significados captados son factores relevantes en el patrón de riesgos para la salud. Se observa también que los procesos sociales relacionados con el género femenino tienen impacto en la salud mental.
Choosing Higher Education: Educationally Ambitious Chicanos and the Path to Social Mobility
Patricia Gandara
Education Policy Analysis Archives , 1994,
Abstract: This is a study of high academic achievement found in the most unlikely places: among low-income Mexican Americans from homes with little formal education. It examines the backgrounds of 50 persons, male and female from one age cohort, who met most of the predictors for school failure or "dropping out." All came from families in which neither parent completed high school or held a job higher than skilled labor; the average father finished grade four and most were sons and daughters of farmworkers and other unskilled laborers. Most began school with Spanish as their primary language, yet all completed doctoral-level educations from the country's most prestigious institutions. This study investigates the forces that conspire to create such anomalies. Its aim is to suggest how such outcomes might be the product of design rather than accident.
Evaluation of culture media for counts of Bifidobacterium animalis in the presence of yoghurt bacteria
Moriya, Juliana;Fachin, Luciano;Gandara, Ana Lourdes Neves;Viotto, Walkiria Hanada;
Brazilian Journal of Microbiology , 2006, DOI: 10.1590/S1517-83822006000400021
Abstract: much attention has been paid to the use of bifidobacterium sp. in yoghurts due to their excellent therapeutic and nutritional properties. however, in order to present health properties, it is recommended that each commercial product should indicate the minimum daily amount required for it to confer health benefit(s) based on in vitro and human studies. besides the inherent low growth of bifidobacterium sp in milk, there is a need for a reliable method for counting bifidobacterium sp in the presence of yoghurt bacteria. this study evaluated the use of the media m-mrs, mrs-nnlp and rcpb ph5 aimed at counting the number of bifidobacterium animalis subesp. lactis in the presence of streptococcus thermophilus and lactobacillus delbrueckii subsp bulgaricus after yoghurt fermentation. the m-mrs medium was not selective, allowing for the growth of l. delbrueckii subsp bulgaricus. mrs-nnlp medium presented a good selectivity for b. animalis bb12 with a slight reduction in the cell count of this microrganism when compared it to the standard mrs medium in pure culture. mrs-nnlp medium was considered a good option to enumerate b. animalis bb12 although the reduction found in pure culture due to the low difference between the counts. the medium rcpb ph5 presented differentiated growth of b. animalis bb12 in relation to the yoghurt bacteria and a cell recovery equal to that of the standard mrs, being considered the best option to enumerate bifidobacterium sp in the presence of yoghurt bacteria.
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