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Search Results: 1 - 10 of 20827 matches for " Amirul Al-Ashraf; Noor "
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PCR assembly of synthetic human erythropoietin gene
Bustami,Yazmin; Yahya,Ahmad Ramli Mohd; Muhammad,Tengku Sifzizul Tengku; Shu-Chien,Alexander Chong; Abdullah,Amirul Al-Ashraf; Noor,Mohd Azizan Mohd; Arip,Yahya Mat;
Electronic Journal of Biotechnology , 2009,
Abstract: human erythropoietin (huepo) is a glycoprotein with important physiological functions, such as erythropoiesis, angiogenesis, and wound healing. a therapeutic protein, huepo is commonly used to treat patients suffering from renal and non-renal anemia. recombinant human erythropoietin (rhuepo) and endogenous huepo are similar with respect to their biological and chemical properties. in this study, we describe the construction of synthetic huepo gene to produce rhuepo. the synthetic huepo gene was constructed by overlapping oligonucleotides assembly and amplified by polymerase chain reaction (pcr). twenty oligonucleotide sets, covering the huepo gene sequence and two newly introduced restriction enzyme sites, were pulled together and amplified using pfu dna polymerase to produce the expected dna products with sizes of ~500bp and ~600bp. the pcr products were ligated into pgem-t plasmid vector to facilitate dna sequencing process of the constructed huepo gene and downstream cloning manipulation. dna sequence analysis showed correctly assembled oligonucleotide sets, representing the huepo gene sequence albeit with minor base mutations. hence, oligonucleotides assembly and pcr amplification provide a convenient and speedy method for the synthesis of huepo gene without depending on mrna isolation and reverse transcription or the need to have a genomic library.
An Intelligent Assessment Tool for Students’ Java Submissions in Introductory Programming Courses  [PDF]
Fatima Al Shamsi, Ashraf Elnagar
Journal of Intelligent Learning Systems and Applications (JILSA) , 2012, DOI: 10.4236/jilsa.2012.41006
Abstract: This paper presents a graph-based grading system for Java introductory programming courses, eGrader. This system grades submission both dynamically and statically to ensure a complete and through grading job. While dynamic analysis is based on JUnit framework, the static analysis is based on the graph representation of the program and its quality which is measured by software metrics. The graph representation is based on the Control Dependence Graphs (CDG) and Method Call Dependencies (MCD). eGrader outperforms existing systems in two ways: the ability of grading submission with semantic-errors, effectively, and generating reports for students, as a feedback on their performance, and instructors on the overall performance of the class. eGrader is well received by instructors not only for saving time and effort but also for its high success rate that is measured by four performance indicators which are sensitivity (97.37%), specificity (98.1%), precision (98.04%) and accuracy (97.07%).
Study of Various Sized Leaf Vascular Bundles and Surrounding Tissues of Six Sugarcane Varieties
Nosheen Noor Elahi,M. Ashraf
Pakistan Journal of Biological Sciences , 2001,
Abstract: Three sized vascular bundles and significant difference in the distance between these vascular bundles and different types of motor cells in the leaf blades of all six sugarcane varieties (i.e., CP84-1198, CP85-1491, CP88-1165, CP89-846, TCP86-3368, CP77-400) were noted and these results indicated the existence of relationship between leaf cytology and morphological characters like curling habit. Large vascular bundles were characterized by the presence of large metaxylem vessels on either side of the protoxylem. The cellular composition of large vascular bundles indicated that the phloem loading in large vascular bundle is an apoplastic step. In some varieties large motor cells were observed. Cytological differences in leaf blade indicated the difference in morpho-physiological characters in all the varieties
A Logarithmic-Complexity Algorithm for Nearest Neighbor Classification Using Layered Range Trees  [PDF]
Ibrahim Al-Bluwi, Ashraf Elnagar
Intelligent Information Management (IIM) , 2012, DOI: 10.4236/iim.2012.42006
Abstract: Finding Nearest Neighbors efficiently is crucial to the design of any nearest neighbor classifier. This paper shows how Layered Range Trees (LRT) could be utilized for efficient nearest neighbor classification. The presented algorithm is robust and finds the nearest neighbor in a logarithmic order. The proposed algorithm reports the nearest neighbor in , where k is a very small constant when compared with the dataset size n and d is the number of dimensions. Experimental results demonstrate the efficiency of the proposed algorithm.
Colluding Jamming Attack on a Grand Coalition by Aggrieved Nodes  [PDF]
Ashraf Al Sharah, Taiwo Oyedare, Sachin Shetty
Communications and Network (CN) , 2016, DOI: 10.4236/cn.2016.82007
Abstract: Mobile Ad-Hoc Networks (MANETs) are highly vulnerable to insider jamming attacks. Several approaches to detect insider jammers in MANET have been proposed. However, once the insider jammer is detected and removed from the network, it is possible for the insider jammer to leverage the knowledge of insider information to launch a future attack. In this paper, we focus on collaborative smart jamming attacks, where the attackers who have been detected as insider jammers in a MANET, return to attack the MANET based on the knowledge learned. The MANET uses a reputation-based coalition game to detect insider jammers. In the collaborative smart jamming attack, two or more smart jammers will form a coalition to attack the coalitions in the MANET. The smart jammers were detected and then excluded from their initial coalition, they then regrouped to start their own coalition and share previously gained knowledge about legitimate nodes in their erstwhile coalition with the aim of achieving a highly coordinated successful jamming attack on the legitimate coalition. The success of the attack largely depends on the insider jammer’s collective knowledge about the MANET. We present a technique to appropriately represent knowledge gathered by insider jammers which would lead to a successful attack. Simulation results in NS2 depict that coalition of jammers can leverage past knowledge to successfully attack MANET.
A Comparative Study on Numerical Solutions of Initial Value Problems (IVP) for Ordinary Differential Equations (ODE) with Euler and Runge Kutta Methods  [PDF]
Md. Amirul Islam
American Journal of Computational Mathematics (AJCM) , 2015, DOI: 10.4236/ajcm.2015.53034
Abstract: This paper mainly presents Euler method and fourth-order Runge Kutta Method (RK4) for solving initial value problems (IVP) for ordinary differential equations (ODE). The two proposed methods are quite efficient and practically well suited for solving these problems. In order to verify the ac-curacy, we compare numerical solutions with the exact solutions. The numerical solutions are in good agreement with the exact solutions. Numerical comparisons between Euler method and Runge Kutta method have been presented. Also we compare the performance and the computational effort of such methods. In order to achieve higher accuracy in the solution, the step size needs to be very small. Finally we investigate and compute the errors of the two proposed methods for different step sizes to examine superiority. Several numerical examples are given to demonstrate the reliability and efficiency.
Evaluation of Exotic Sugarcane Germplasm for Agronomic Characters and Productivity
Maqbool Akhtar,Nosheen Noor Elahi,M. Ashraf
Pakistan Journal of Biological Sciences , 2001,
Abstract: Six exotic sugarcane varieties were evaluated for their agronomic characters and productivity. All exotic sugarcane varieties had similar germination percentage except standard variety CP77-400 which had higher germination. Significant differences were found in number of tillers ha -1, stalk height, girth and total soluble solids. Minimum (102.3 thousands ha -1) number of millable canes were found in variety CP84-1198 and the maximum (160 thousands ha -1) in CP85-1491. Cane yield ranged from the minimum of 70.67 t ha -1 in variety CP84-1198 to the maximum of 131.3 t ha -1 in variety TCP86-3368. Sugar recovery was maximum (13.78 %) in variety CP85-1491 and minimum (11.82%) in the variety TCP86-3368. Sugarcane variety CP84-1198 produced the minimum (9.26 t ha -1) sugar yield. Purity of juice was also significantly different in most of the varieties. All the varieties except CP 84-1198 had better cane and sugar yield potentials than the standard variety.
Morphological Characters of Some Exotic Sugarcane Varieties
Maqbool Akhtar,Nosheen Noor Elahi,M. Ashraf
Pakistan Journal of Biological Sciences , 2001,
Abstract: Six exotic varieties of sugarcane (CP84-1198, CP85-1491, CP88-1165, CP77-400, CP89-846, TCP86-3368) planted at the National Agricultural Research Centre during 1998-99 were described for their morphological characters. All the varieties greatly varied in different characters. Mean leaf length, number of green leaves and width of leaf lamina/blade was different in different varieties. These varieties also differed in leaf sheath colour, carriage, and other leaf characters. Shape of the dewlap, its size and colour, shape of ligule and ligular process or auricle was also different. All the varieties varied in diameter of the stalks, colour of the stalks, shape of internode, bud groove characters, width and colour of growth ring, width and colour of root zone and wax band characters. Ivory markings were present on stalks of all the varieties. All the varieties were similar in bud size, but different in shapes of the bud, some having flanges and some without flanges. Position of the germ pore on the bud was also different in all the varieties.
Path Analysis and Relationship Among Quantitative Traits in Chickpea ( Cicer arietinum L.)
Falak Noor,Muhammad Ashraf,Abdul Ghafoor
Pakistan Journal of Biological Sciences , 2003,
Abstract: Phenotypic and genotypic variances, heritability in broad sense (h2), genetic advance (GA), correlation and path coefficient analyses were conducted for yield and yield components in 30 genotypes of chickpea under rainfed conditions. Medium to high genetic variance was observed for days to flowering, maturity, secondary branches and 100-seeds weight, whereas for other characters, low to medium heritablity (broad sense) was observed along with low to high genetic advance. Improvement of these traits thorough direct selection could be limited from germplasm used in the present study. Days to flowering, days to maturity and 100-seed weight exhibited high heritablity coupled with high genetic advance, hence crop improvement through these traits could be possible by simple selection. It was concluded that to improve grain yield emphasis should be given on development of chickpea cultivars with higher seed weight and biological yield. To break undesirable linkage of biological yield Vs harvest index and grain yield Vs harvest index, bi-parental mating among selective parents is suggested for further improvement. Both correlation and path analyses indicated that pods per plant and 100-seed weight were potent contributors to grain yield through direct effects. Seven elite accessions which produced higher grain yield than both the checks were selected and suggested for further evaluation under various ecological zones for adaptation.
Two solutions for the BVP of a rotating variable-thickness solid disk  [PDF]
Ashraf M. Zenkour, Suzan A. Al-Ahmadi
Natural Science (NS) , 2011, DOI: 10.4236/ns.2011.32021
Abstract: This paper presents the analytical and numerical solutions for a rotating variable-thickness solid disk. The outer edge of the solid disk is considered to have free boundary conditions. The governing equation is derived from the basic equations of the rotating solid disk and it is solved analytically or numerically using finite difference algorithm. Both analytical and numerical results for the distributions of stress function and stresses of variable-thickness solid disks are obtained. Finally, the distributions of stress function and stresses are presented and the appropriate comparisons and discussions are made at the same angular velocity.
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