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Search Results: 1 - 10 of 10 matches for " Alongkorn Yoosamran "
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Recent Electrochemical and Optical Sensors in Flow-Based Analysis
Orawon Chailapakul,Passapol Ngamukot,Alongkorn Yoosamran,Weena Siangproh,Nattakarn Wangfuengkanagul
Sensors , 2006, DOI: 10.3390/s6101383
Abstract: Some recent analytical sensors based on electrochemical and optical detectioncoupled with different flow techniques have been chosen in this overview. A briefdescription of fundamental concepts and applications of each flow technique, such as flowinjection analysis (FIA), sequential injection analysis (SIA), all injection analysis (AIA),batch injection analysis (BIA), multicommutated FIA (MCFIA), multisyringe FIA(MSFIA), and multipumped FIA (MPFIA) were reviewed.
Review on Micro- and Nanolithography Techniques and Their Applications
Alongkorn Pimpin,Werayut Srituravanich
Engineering Journal , 2012, DOI: 10.4186/ej.2012.16.1.37
Abstract: This article reviews major micro- and nanolithography techniques and their applications from commercial micro devices to emerging applications in nanoscale science and engineering. Micro- and nanolithography has been the key technology in manufacturing of integrated circuits and microchips in the semiconductor industry. Such a technology is also sparking a magnificent transformation of nanotechnology. The lithography techniques including photolithography, electron beam lithography, focused ion beam lithography, soft lithography, nanoimprint lithography and scanning probe lithography are discussed. Furthermore, their applications are reviewed and summarized into four major areas: electronics and microsystems, medical and biotech, optics and photonics, and environment and energy harvesting.
NSP2 gene variation of the North American genotype of the Thai PRRSV in central Thailand
Roongtham Kedkovid, Suparlark Nuntawan Na Ayudhya, Alongkorn Amonsin, Roongroje Thanawongnuwech
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-340
Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen causing major economic losses in the swine industry worldwide. PRRSV is an enveloped virus with an ssRNA genome of positive polarity belonging to the order Nidovirales, family Arteriviridae, genus Arterivirus. PRRSV has been genetically divided into 2 genotypes, the European (Type 1) and North American (Type 2) genotype, respectively. Both genotypes are highly diverse, sharing only approximately 60% nucleotide identity [1,2]. However, genetic variations within each genotype are also highly observed [3,4]. The PRRSV genome is approximately 15 kb in length and comprises 9 open reading frames (ORFs), ORF1a, ORF1b, ORF2a, ORF2b, ORF3, ORF4, ORF5, ORF6 and ORF7. ORF1a and ORF1b (~12 kb) encode 12 non-structural proteins (nsp), nsp1 - nsp12, which play major roles in viral replication [5-7]. The remaining ORFs encode structural proteins [8,9].The nsp2-coding region is genetically the most variable area and crucial for viral replication due to its protease activity [10]. For nsp generation, as shown with the equine arteritis virus (EAV, a prototype virus of the genus Arterivirus) model, ORF1 is primarily translated into ORF1a and ORF1b polyprotein, and these 2 proteins are then cleaved into nsp1 - 8 and nsp1 - 12, respectively [11].Recently, Type 2 PRRSV with a nucleotide deletion in the nsp2 coding region has been identified in USA, China, Japan, Denmark and Vietnam [4,12-15]. Following the outbreaks of swine high fever (SHF) syndrome in China, many genetic variants of the virus have been isolated. A novel nucleotide deletion in nsp2 found in those Chinese isolates was initially linked to the virulence of the virus [14]. The objective of this study was to investigate the deletion patterns of Type 2 PRRSV found in Thailand. Nine recent Thai isolates of Type 2 PRRSV (2007-2008), 07NP2, 07NP4, 78/51, 8NP46, 8NP154, 08RB1, 8NP147, 8NP148 and 8NP59 and one previous Thai isolate (01CS1/2) obta
Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes)
Alongkorn Amonsin, Roongtham Kedkovid, Suphasawatt Puranaveja, Piya Wongyanin, Sanipa Suradhat, Roongroje Thanawongnuwech
Virology Journal , 2009, DOI: 10.1186/1743-422x-6-143
Abstract: 01CB1 and 01NP1 contain 14,943 and 15,412 nucleotides, respectively. The viruses compose 2 untranslated regions (5' UTR and 3' UTR) and 8 open reading frames (ORFs) designated as ORF1a, ORF1b and ORF2-7. Phylogenetic analysis of full length of the viruses also showed that the 01CB1 and 01NP1 were grouped into the EU and US genotype, respectively. In order to determine the genetic variation and genetic relatedness among PRRSV isolates, the complete nucleotide sequences of PRRSV isolated in Thailand, 01CB1 and 01NP1 were compared with those of 2 EU strains (Lelystad, and EuroPRRSV), 6 US strains (MLV, VR2332, PA8, 16244B, SP and HUN4). Our results showed that the 01CB1 genome shares approximately 99.2% (Lelystad) and 95.2% (EuroPRRSV) nucleotide identity with EU field strains. While, the 01NP1 genome has 99.9% nucleotide identity with a live vaccine strain (MLV) and 99.5% and 98.5% nucleotide identity with 2 other US isolates, VR2332 and 16244B, respectively. In addition, ORF5 nucleotide sequences of 9 PRRS viruses recovered in Thailand during 2002-2008 were also included in this study. Phylogenetic analysis of ORF5 showed high similarity among EU and US genotypes of the recent Thai PRRS viruses (2007-2008 viruses) with 01CB1 and 01NP1.Overall, the results suggested that the Thai EU isolate (01CB1) may evolve from the EU prototype, Lelystad virus, whereas the Thai US isolate (01NP1) may originate and evolve from the vaccine virus or its derivatives. Interestingly, the US-MLV vaccine was not available in the Thai market in 2001. The Vaccine-like virus might have persisted in the imported pigs or semen and later spread in the Thai swine industry. This report is the first report of complete nucleotide sequences of the Thai PRRS viruses both EU and US genotypes.Porcine reproductive and respiratory syndrome virus (PRRSV) belonging to the genus Arterivirus in the family Arteriviridae in the order Nidovirales is a major swine virus causing economic losses in the swine indust
Genetic characterization of avian influenza subtype H4N6 and H4N9 from live bird market, Thailand
Trong Wisedchanwet, Manoosak Wongphatcharachai, Supanat Boonyapisitsopa, Napawan Bunpapong, Pravina Kitikoon, Alongkorn Amonsin
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-131
Abstract: Live-bird markets (LBMs) are the places where wild birds, pet birds, meat birds and domestic poultry are sold to households. In Asia including Thailand, due to the cultural preference of consuming freshly slaughtered poultry, LBMs are located in both suburban areas and center of the communities. In the markets, thousands of birds from different sources are sold in wire stacked cages containing densely packed and mixed bird populations. These conditions provide excellent environments for animal to animal and animal to human influenza virus transmissions and may result in an outbreak of influenza A virus in both animals [1,2] and humans [3,4]. Therefore, LBMs are considered a major source of influenza A virus dissemination and potential influenza A virus reassortment [5,6].Up to date, many studies on influenza A in LBMs from various countries have been reported. During 2000-2001, 6 subtypes (9 genotypes) of low-pathogenic avian influenza (LPAI) were identified in LBMs in China [7]. Apart from Asian countries, in the US, H5N2 low pathogenic avian influenza (LPAI) viruses have been isolated from LBMs in several states in the 80 s [8]. In Thailand, only one study of influenza A viruses recovered from LBMs has ever been reported [9]. In that study, highly pathogenic avian influenza (HPAI) H5N1 viruses were isolated from both bird carcasses and healthy birds during the 2006-2007 LBM and local food market (LFM) surveillance program. The findings suggested that animal movement from H5N1 outbreak areas may introduce the virus into the markets and play an important role in emergence or re-emergence of influenza A in animals in Thailand [9]. Since LBMs play an important role in the dissemination of avian influenza virus, active surveillance of influenza A virus in LBMs is important in order to develop an early warning system and implement prevention and control strategies for influenza A outbreaks. In this study, a one year active surveillance program for influenza A viruses am
Use of Repetitive Sequence-Based Polymerase Chain Reaction to Characterize Streptococcus equi Subspecies Equi Isolates Cultured from Horses Experiencing Post-Vaccinal Reaction
Ghanem M. Al-Ghamdi,Alongkorn Amonsen,Connie J. Gebhart,Vivek Kapur,Trevor R. Ames
Journal of Animal and Veterinary Advances , 2012,
Abstract: Strangles caused by Streptococcus equi subspecies equi is a continuous challenge to horse owners and practitioners. This led to great effort in developing vaccines against this disease. The goal of this study was to characterize S. equi isolates obtained from horses suffering from post vaccinal reactions following the use of an intranasal vaccine Pinnacle INTM, Fort Dodge Animal Health. Fifteen S. equi isolates obtained from several distinct post vaccinal reaction episodes in Colorado, Florida, Kansas, Kentucky, Minnesota and Ohio, USA. Clinical signs in these post vaccinal reactions episodes included fever, localized neck abscesses, strangles, or purpura hemorrhagica. S. equi was cultured from neck abscesses and/or lymph nodes. Repetitive element based polymerase chain reaction (rep-PCR) was performed on the post vaccinal reaction isolates and the vaccine strain. DNA analysis showed that all of the post vaccinal reaction isolates except one obtained from various geographic locations were identical to the intranasal vaccine strain. One isolate was found to be genetically distinct from the intranasal vaccine strain using the rep-PCR. These findings clearly suggest that the horses exhibited complication as a result of vaccination except in one incidence. Whether these complications occurred from a return to virulence by the organism, immune reaction of the host or inappropriate administration of the vaccine is unclear.
Comparative analysis of Mycobacterium avium subsp. paratuberculosis isolates from cattle, sheep and goats by short sequence repeat and pulsed-field gel electrophoresis typing
Iker Sevilla, Lingling Li, Alongkorn Amonsin, Joseba M Garrido, Maria V Geijo, Vivek Kapur, Ramón A Juste
BMC Microbiology , 2008, DOI: 10.1186/1471-2180-8-204
Abstract: A total of nineteen different multi-locus SSR (SSR1_SSR8) types were identified amongst the 268 isolates compared to the 37 multiplex profiles differentiated by the SnaBI-SpeI PFGE. SSR type 7_4 was the predominant genotype (51.2% of all isolates and 54.3% of cattle isolates), but combined with PFGE results the abundance of the most prevalent genotype (7_4&{2-1}) dropped down to 37.7%. SSR types 7_3 and 14_3 were significantly spread amongst isolates recovered from small ruminants. The comparison of SSR1_SSR8 and SnaBI-SpeI PFGE typing of these isolates has shown that both methods perform at similar discriminatory level. These were 0.691 and 0.693, respectively for SSR and PFGE as indicated Simpson's Index of Diversity, and 0.82 when calculated for combined SSR and PFGE genotypes. Overall, SSR1_SSR8 analysis seemed to detect higher levels of within-farm strain diversity and seemed to give higher year-related information. Combination of both typing methods revealed 20 multi-type farms out of the 33 bovine farms studied with more than one isolate.The particular SSR and PFGE typing approaches described here are in general agreement but they showed some discrepancies that might reflect differing evolutionary processes of Map strains. Both methods are able to reciprocally complement their results and neither should be replaced with the other if sufficient material and time is available. Overall, the results of our comparative analyses suggest that, based on current methodologies available, a combined approach that includes SSR and PFGE seems to provide the highest level of discrimination for Map strain typing with meaningful epidemiological information.Mycobacterium avium subsp. paratuberculosis (Map) is the causative agent of paratuberculosis, a chronic digestive disease affecting mainly bovine, ovine, caprine and cervine livestock. Although the aetiology of Crohn's Disease has been subject of strong controversy [1,2], recent information seems to confirm an association
Genetic variations of nucleoprotein gene of influenza A viruses isolated from swine in Thailand
Nattakarn Thippamom, Donreuthai Sreta, Pravina Kitikoon, Roongroje Thanawongnuwech, Yong Poovorawan, Apiradee Theamboonlers, Kamol Suwannakarn, Sujira Parchariyanon, Sudarat Damrongwatanapokin, Alongkorn Amonsin
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-185
Abstract: Twelve influenza A virus specimens were isolated from Thai swine. All samples were subjected to nucleotide sequencing of the complete NP gene. Phylogenetic analysis was conducted by comparing the NP gene of swine influenza viruses with that of seasonal and pandemic human viruses and highly pathogenic avian viruses from Thailand (n = 77). Phylogenetic analysis showed that the NP gene from different host species clustered in distinct host specific lineages. The NP gene of swine influenza viruses clustered in either Eurasian swine or Classical swine lineages. Genetic analysis of the NP gene suggested that swine influenza viruses circulating in Thailand display 4 amino acids unique to Eurasian and Classical swine lineages. In addition, the result showed 1 and 5 amino acids unique to avian and human lineages, respectively. Furthermore, nucleotide substitution rates showed that the NP gene is highly conserved especially in avian influenza viruses.The NP gene sequence of influenza A in Thailand is highly conserved within host-specific lineages and shows amino acids potentially unique to distinct NP lineages. This information can be used to investigate potential interspecies transmission of influenza A viruses. In addition, the genetic variations of the NP gene will be useful for monitoring the viruses and preparing effective prevention and control strategies for potentially pandemic influenza outbreaks.Influenza A virus poses a serious threat to public health worldwide, particularly the virus circulating in humans and animal species such as birds, pigs and horses. Influenza A subtypes H1-3 and N1-2 have been circulating in the human population, while Influenza A subtypes H1 and 3 and N1-2 have been reported in swine. On the other hand, all H1-16 and N1-9 can be found in avian species [1,2]. The virus genome contains 8 segments of single-stranded RNA that encode 10-11 proteins. Among those genes, the NP gene plays a major role with regard to host range or host species barri
Genetic diversity and multiple introductions of porcine reproductive and respiratory syndrome viruses in Thailand
Hein M Tun, Mang Shi, Charles LY Wong, Suparlark NN Ayudhya, Alongkorn Amonsin, Roongroje Thanawonguwech, Frederick CC Leung
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-164
Abstract: PRRSV is a major swine disease virus causing economic losses in swine industry worldwide including Thailand. This disease was first reported during almost concurrent epidemics in the North American countries (late 1980s) [1,2] and in the European countries (early 1990) [3]. The causative agent, porcine reproductive and respiratory syndrome virus (PRRSV) belongs to the family Arteriviridae in the order Nidovirales [4]. It is an enveloped virus containing a positive-sense RNA genome of approximately 15 kb in length, encoding at least nine open reading frames (ORFs) including ORF1a, 1b, 2a, 2b, and 3-7 [5]. Among them, ORF5 encoding the major envelope protein is often used for phylogenetic analysis because of its high variability. PRRSV can be divided into two major genotypes: Type 1 (European strains) and Type 2 (North American strains). Both genotypes are found to be genetically and antigenically heterogeneous [6,7]. According to the recent reports for PRRSV classification, Type 1 PRRSV is divided into 3 subtypes. Among them, the cosmopolitan Subtype I was further divided into 12 different clades [8,9]. For Type 2 PRRSV, 9 well-defined lineages have been described [8,10].PRRSV has been circulating in Thailand for a long time. Seropositive animals could be traced back to as early as 1989, and seropositive rate increased annually from 1991 to 2002 [11]. In 1996, the first Thai PRRSV was isolated and was identified as Type 2 PRRSV [12]. A few years later, Type 1 PRRSV was also reported in Thailand, and some was found to be co-circulating with Type 2 PRRSV within the same herd [11]. Despite of the effort on characterizing PRRSV diversity in Thailand, the origin and epidemiological history of the viruses remain unknown. This study re-analyzes previously characterized Thai sequences in the context of global PRRSV diversity, which helps shed lights on the potential origin and prevalence of different variants of PRRSVs in Thailand.All Thai PRRS viruses were isolated from nur
Genetic characterization of 2008 reassortant influenza A virus (H5N1), Thailand
Alongkorn Amonsin, Jiradej Lapkuntod, Kamol Suwannakarn, Pravina Kitikoon, Sanipa Suradhat, Rachod Tantilertcharoen, Supanat Boonyapisitsopa, Napawan Bunpapong, Manoosak Wongphatcharachai, Trong Wisedchanwet, Apiradee Theamboonlers, Yong Poovorawan, Jiroj Sasipreeyajan, Roongroje Thanawongnuwech
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-233
Abstract: H5N1 influenza A virus has caused avian influenza (AI) outbreaks worldwide. In Thailand, 7 major AI outbreaks have been reported since early 2004 [1-3]. In January 2008, outbreaks of H5N1 virus occurred in two provinces, Nakhon Sawan and Phichit. The outbreak in Nakhon Sawan affected 60,000 birds in a broiler farm and chicken in nearby backyards, while the outbreak in Phichit occurred among backyard chicken. In November 2008, H5N1 outbreaks were also reported in two provinces, Sukhothai and Uthai Thani. Both outbreaks occurred among backyard poultry in villages (Fig 1). Currently, at least two clades of influenza A virus (H5N1) have been reported in Thailand including clade1 viruses which are predominant in lower-north and central Thailand and clade2.3.4 viruses which are predominant in northeast Thailand [1,3,4]. Clade1 H5N1 viruses in Thailand have been further divided into 3 distinct subclades including the original clade1 (CUK2-like), clade1.p1 (PC168-like) and clade1.p2 (PC170-like) [3,5]. One study has documented evidence of genetic reassortment of H5N1 viruses in Thailand in 2007 [6]. In this study, we have comprehensively characterized the 2008 H5N1 viruses recovered during the 6th and 7th waves of AI outbreaks in Thailand. The 2008 H5N1 viruses were compared with H5N1 isolates obtained from each wave of AI outbreaks in Thailand. The whole genome sequences of the viruses were analyzed for nucleotide identity, genetic relatedness, virulence determinants, and possible sites of reassortment among H5N1 viruses.Eight H5N1 viruses were isolated from Nakhon Sawan (n = 3), Phichit (n = 1), Sukhothai (n = 2) and Uthai Thani (n = 2) (Table 1 and Fig 1). The viruses were isolated by embryonated egg inoculation [7]. All 8 viruses were confirmed as Influenza A virus subtype H5N1 by real-time-RT-PCR [8]. Whole genome sequences were obtained as previously described [9]. Phylogenetic and genetic relatedness analyses were conducted using the MEGA 4.0 program applying the nei
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