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Search Results: 1 - 10 of 6723 matches for " Alicia; Amérigo "
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Gastrointestinal stromal tumors (GISTs): role of CD 117 and PDGFRA Golgi-like staining pattern in the recognition of mutational status Tumores del estroma gastrointestinal (GISTs): patrón de tinción tipo Golgi de CD 117 Y PDGFRA en el reconocimiento del estado mutacional
Sara Jaramillo,María José Ríos-Moreno,Alicia Hernández,Joaquín Amérigo
Revista Espa?ola de Enfermedades Digestivas , 2012,
Abstract: Aims: determine whether potential correlations between CD117 to and PDGFRA might serve as an indication for targeted therapies. Material and methods: immunohistochemical expression of CD117 and PDGFRA was evaluated in 99 paraffin-embedded GISTs in conjunction with KIT and PDGFRA mutational status. Results: CD117-positive staining was noted in 93 out of 99 cases. The predominant staining pattern was cytoplasmic, either with or without membrane accentuation; in 44.5% of cases, a clear Golgi-like pattern was evident. Correlations were found be-tween KIT mutation and both CD117 expression (p = 0.006) and Golgi-like pattern (p = 0.026). Cytoplasmic PDGFRA-positive staining was detected in 87% of cases, both with and without membrane accentuation; in 8% cases an evident Golgi-like staining pattern was observed. A significant correlation was noted between PDGFRA mutations and Golgi-like staining pattern (p = 0.001). Moreover, 95% of PDGFRA-positive GISTs were also CD117-positive, suggesting that expression of the two markers is not mutually exclusive; most of these had mutations in KIT exon 11. PDGFRA-positive/CD117-negative tumors had mutations in PDGFRA, mainly in exon 18. PDGFRA-negative/CD117-negative staining was observed in 15% of cases, all of which displayed mutations in KIT exon 11. CD117-positive/PDGFRA-negative cases were characterized by mutations in KIT, mainly in exon 11. Conclusions: CD117 and PDGFRA staining are not exclusive, and the presence of a Golgi-like staining pattern for either, whilst not pathognomonic, is highly suggestive of KIT and PDGFRA mutated GISTs, respectively, and may be used with some reservations as an alternative indication for prescribing targeted therapies. Objetivo: determinar si las posibles correlaciones entre CD117 y PDGFRA podrían servir como una indicación de terapias dirigidas. Material y métodos: la expresión inmunohistoquímica de CD117 y PDGFRA se evaluó en 99 GIST incluidos en parafina en conjunción con el estado mutacional de KIT y PDGFRA Resultados: se observó tinción CD117-positivo en 93 de los 99 casos. El patrón de tinción predominante fue citoplasmático o de membrana; en el 44,5% de los casos, se evidencio patrón de tipo Golgi. Se encontraron correlaciones entre la mutación KIT tanto con la expresión de CD117 (p = 0,006) y con el patrón tipo Golgi (p = 0,026). Se detectó tinción citoplasmática PDGFRA-positiva en el 87% de los casos, con y sin acentuación de membrana, en el 8% se observó patrón de tinción tipo Golgi. Se observó una correlación significativa entre las mutaciones PDGFRA y el patrón de tinció
Gastrointestinal stromal tumors (GISTs): role of CD 117 and PDGFRA Golgi-like staining pattern in the recognition of mutational status
Jaramillo,Sara; Ríos-Moreno,María José; Hernández,Alicia; Amérigo,Joaquín; Trigo-Sánchez,Inmaculada; González-Cámpora,Ricardo;
Revista Espa?ola de Enfermedades Digestivas , 2012, DOI: 10.4321/S1130-01082012000300005
Abstract: aims: determine whether potential correlations between cd117 to and pdgfra might serve as an indication for targeted therapies. material and methods: immunohistochemical expression of cd117 and pdgfra was evaluated in 99 paraffin-embedded gists in conjunction with kit and pdgfra mutational status. results: cd117-positive staining was noted in 93 out of 99 cases. the predominant staining pattern was cytoplasmic, either with or without membrane accentuation; in 44.5% of cases, a clear golgi-like pattern was evident. correlations were found be-tween kit mutation and both cd117 expression (p = 0.006) and golgi-like pattern (p = 0.026). cytoplasmic pdgfra-positive staining was detected in 87% of cases, both with and without membrane accentuation; in 8% cases an evident golgi-like staining pattern was observed. a significant correlation was noted between pdgfra mutations and golgi-like staining pattern (p = 0.001). moreover, 95% of pdgfra-positive gists were also cd117-positive, suggesting that expression of the two markers is not mutually exclusive; most of these had mutations in kit exon 11. pdgfra-positive/cd117-negative tumors had mutations in pdgfra, mainly in exon 18. pdgfra-negative/cd117-negative staining was observed in 15% of cases, all of which displayed mutations in kit exon 11. cd117-positive/pdgfra-negative cases were characterized by mutations in kit, mainly in exon 11. conclusions: cd117 and pdgfra staining are not exclusive, and the presence of a golgi-like staining pattern for either, whilst not pathognomonic, is highly suggestive of kit and pdgfra mutated gists, respectively, and may be used with some reservations as an alternative indication for prescribing targeted therapies.
Anticuerpos anti-ribosomales como marcadores de actividad en el LES
Almeida,D.; Antolín,J.; Amérigo,M. J.; Cantabrana,A.; Roces,A.; Hayeck,M.;
Anales de Medicina Interna , 2002, DOI: 10.4321/S0212-71992002000200005
Abstract: objectives: a) to determine the prevalence of anti-ribosomal p antibodies in patients with esl in our setting; b) to determine if there are associations between clinical signs of esl and these autoantibodies; c) to analyze if there is any correlation between the presence of anti-p in patients with esl and the results of other routine lab tests; and d) to assess the usefulness of implementing as routine test the determination of anti-p antibodies. material and methods: the study included 60 patients diagnosed of esl and 61 healthy subjects as the control group. elisa was used to determine anti-ribosomal antibodies. chi-square, fisher and student t tests were used for the statistical analyses. results: of the 60 patients with sle, 29 (48%) had anti-p antibodies as determined by elisa. no association was observed between the presence of anti-p antibodies and psychosis, depression, hepatic failure, renal failure or any other clinical signs of esl. a correlation was found between the levels of anti-p antibodies as determined by elisa and anti-histone, ana and ama antibodies. conclusions: the prevalence of anti-p antibodies was high among our esl patients (48%). their presence was not significantly associated with any clinical sign; however, an association was found with other lab markers related to the presence of active disease.
Utilización de las TIC en el aprendizaje autónomo del estudiante: aplicación en la asignatura Fisioterapia Cardiocirculatoria Use of ICT in the Autonomous Learning Process: A Case Study in Cardiocirculatory Physiotherapy Utilització de les TIC en l'aprenentatge autònom de l'estudiant: aplicació en l'assignatura de Fisioteràpia Cardiocirculatoria
Maria àngels Cebrià i Iranzo,Enrique Ricardo Mora Amérigo,Celedonia Igual Camacho
@tic : Revista d'Innovació Educativa , 2009, DOI: 10.7203/attic.3.154
Abstract: El Espacio Europeo de Educación Superior (EEES) promueve un cambio metodológico en los estudios universitarios, al que sin duda contribuye el uso de las tecnologías de la información y la comunicación (TIC). En este artículo presentamos un material multimedia desarrollado en el contexto de la titulación de Fisioterapia, cuya finalidad es guiar y reforzar el aprendizaje del estudiante, tanto dentro como fuera del aula. La valoración del material por parte de los estudiantes en el aula mediante un estudio piloto puso de manifiesto tanto ventajas como inconvenientes en relación a las metodologías planteadas y el formato utilizado. The European Higher Education Area (EHEA) promotes a methodological change in the university degrees, improved with no doubt with the help of the information and communication technologies (ICT). In the following article we present a multimedia material developed in the context of the Physiotherapy degree, whose goal is to guide and reinforce the student’s learning, both inside and outside the classroom. The students’ assessment of the material in the classroom through a pilot study showed us advantages and improvements related to the applied methodologies and the chosen format. L’Espai Europeu d’Educació Superior (EEES) promou un canvi metodològic en els estudis universitaris, al qual contribueix sense dubte l’ús de les tecnologies de la informació i de la comunicació (TIC). En aquest article presentem un material multimèdia desenvolupat en el context de la titulació de Fisioteràpia, la finalitat del qual és guiar i refor ar l’aprenentatge de l’estudiant, tant dins com fora de l’aula. La valoració del material per part dels estudiants a l’aula mitjan ant un estudi pilot va fer palès tant avantatges com punts de millora, en relació a les metodologies plantejades i el format emprat.
Interactividad, información, promoción y valor de portal de las televisiones locales en la Red
Lic. Elvira García de Torres,Lic. María José Pou Amérigo
Revista Latina de Comunicación Social , 2000,
Abstract: Tomamos como referencia un trabajo preliminar, presentado en el congreso Comunicar en la Era Digital, que examina la interactividad y la información de las páginas web de las televisiones locales valencianas a partir de un programa de codificación de 72 variables, que se aplica a las páginas de nueve televisiones locales valencianas, la semana del 14 al 21 de febrero de 1999: TVV-Canal 9, Televisión de Elche, Tele-Safor, Televisión de Villena, Canal 55 de Benidorm, Canal Castelló, Valencia-TeVe, Televisión de Silla y Ribera Televisión (Alzira).
Comparación de dos métodos de fraccionamiento físico de la materia orgánica del suelo
Irizar,Alicia; Andriulo,Adrián; Cosentino,Diego; Améndola,Catalina;
Ciencia del suelo , 2010,
Abstract: regression analysis was used to compare particulate organic carbon (cop) and light carbon (cs) concentrations obtained by size (fraction > 53 μm) and density fractionation (liquor density = 2 g cm-3) methods, respectively. the soil samples were taken from a long-term experiment located at the eea-inta pergamino comparing four cropping sequences and two tillage systems. both methods were linearly and positively correlated to depths of 0-5 and 5-10 cm and the y-intercept did not differ from zero, indicating that both methods resulted in a similar estimate for labile c. as the slopes did not different from unity, the differences between the two methods remained constant throughout all the labile range measured. there was no correlation for a depth of 10-20 cm, and cs concentrations were higher and less variable than those of cop. the liquor density used might explain the obtention of similar or greater amounts of cs than of cop because of the important contribution of the organomineral complex to the light fraction.
Influenza. Vacunas clásicas y novedosas a las puertas de otra pandemia
Raiza Martínez,Nevis Amín,Alicia Aguilar,Frank Camacho
Vaccimonitor , 2006,
Abstract: Los virus de la influenza A, además del hombre, infectan otros mamíferos y una gran variedad de especies de aves. Desde 1997 se sabeque cepas aviares son capaces de infectar al hombre provocando una enfermedad generalmente leve. El brote actual de gripe aviarH5N1 en humanos ha puesto en alerta a la comunidad científica internacional, no solo por su elevada mortalidad sino por supotencialidad en la generación de una nueva pandemia. Las autoridades sanitarias han puesto en marcha un amplio programa para lapreparación ante esta contingencia, que abarca diferentes campos, desde el diagnóstico y la detección precoz de los brotes tanto enanimales como en humanos, la caracterización sistemática de las cepas circulantes, el sacrificio de aves infectadas, la producciónmasiva de antivirales y por supuesto el desarrollo y producción a gran escala de vacunas eficaces. En este último tema se trabajaintensamente tanto en el mejoramiento de las vacunas ya existentes como en la búsqueda de alternativas basadas en tecnologías denueva generación.
Cromatografía de exclusión molecular como metodología para la purificación de bacteriófagos
Frank Camacho,Yaquelín Puchades,Alicia Aguilar,Nevis Amín
Vaccimonitor , 2012,
Abstract: La cromatografía de exclusión molecular puede ser usada para purificar fagos filamentosos, el virus bacteriano más usado en la tecnología de presentación de péptidos y proteínas en la superficie de los fagos. El bacteriófago,luego de ser precipitado del sobrenadante de cultivo con polietilenglicol, fue aplicado a una columna de Sepharose CL-4B de 100 cm de altura, usando como fase móvil solución tampón fosfato (PBS). Se obtuvo un 61,3% de recobrado del fago total aplicado y, mediante el análisis por SDS-PAGE, se verificó la pureza de estas preparaciones.La cromatografía de exclusión molecular es una metodología atractiva para la obtención de preparaciones de bacteriófagos con alta pureza.
Comparación de dos métodos de fraccionamiento físico de la materia orgánica del suelo Comparison of two physical fractionation methods for soil organic matter
Alicia Irizar,Adrián Andriulo,Diego Cosentino,Catalina Améndola
Ciencia del Suelo , 2010,
Abstract: Se compararon las concentraciones de carbono orgánico particulado (COP) y carbono sobrenadante (Cs) obtenidas por los métodos de fraccionamiento granulométrico (fracción > 53 μm) y densimétrico (densidad de licor: 2 g cm-3), respectivamente, utilizando el análisis de regresión. Las muestras de suelo fueron tomadas de un ensayo de larga duración ubicado en la EEA-INTA Pergamino que compara cuatro secuencias de cultivo y dos sistemas de labranza. Los dos métodos estuvieron lineal y positivamente correlacionados para las profundidades 0-5 y 5-10 cm y las ordenadas al origen no difirieron de cero, indicando que ambos estimaron el carbono lábil de manera similar. Debido a que las pendientes no fueron distintas de uno, las diferencias entre los dos métodos fueron constantes en todo el rango de carbono lábil medido. Para la profundidad 10-20 cm, no hubo correlación y las concentraciones de Cs fueron superiores y menos variables que las de COP. La densidad del licor utilizada puede explicar la obtención de cantidades similares o superiores de Cs con respecto a COP, debido a una contribución importante del complejo órgano-mineral a la fracción sobrenadante. Regression analysis was used to compare particulate organic carbon (COP) and light carbon (Cs) concentrations obtained by size (fraction > 53 μm) and density fractionation (liquor density = 2 g cm-3) methods, respectively. The soil samples were taken from a long-term experiment located at the EEA-INTA Pergamino comparing four cropping sequences and two tillage systems. Both methods were linearly and positively correlated to depths of 0-5 and 5-10 cm and the y-intercept did not differ from zero, indicating that both methods resulted in a similar estimate for labile C. As the slopes did not different from unity, the differences between the two methods remained constant throughout all the labile range measured. There was no correlation for a depth of 10-20 cm, and Cs concentrations were higher and less variable than those of COP. The liquor density used might explain the obtention of similar or greater amounts of Cs than of COP because of the important contribution of the organomineral complex to the light fraction.
Coronary flow reserve in stress-echo lab. From pathophysiologic toy to diagnostic tool
Fausto Rigo
Cardiovascular Ultrasound , 2005, DOI: 10.1186/1476-7120-3-8
Abstract: Normally coronary blood flow can increase approximately four-to-six fold to meet increasing myocardial oxygen demands. This effect is mediated by vasodilation at the arteriolar bed, which reduces vascular resistance, thereby augmenting flow. The coronary reserve (CFR) represents the capacity of the coronary circulation to dilate following an increase in myocardial metabolic demands and can be expressed by the difference between the hyperemic flow and the resting flow curve (figure 1). In 1974, Lance K Gould [1] proposed the relationship between the anatomic condition and behaviour of coronary hyperaemic flow (figure2), whereby an inverse curvilinear relationship exists between the narrowing of lumen of coronary artery and hyperaemic capability, up to a completely abolished coronary reserve for stenosis >90%. This experimental paradigm can be accurately reproduced clinically in highly selected series of patients with single vessel disease, no myocardial infarction, no coronary collateral circulation, normal baseline function, no left ventricular hypertrophy, without evidence of coronary vasospasm, and off therapy at the time of testing. The perfect, predictable relationship found in the experimental animal or in a very selected patient population [2] is not so perfect in clinical practice [3,4], where many variables can modulate the imperfect match between epicardial coronary artery stenosis and coronary flow reserve (figure 2), such as the geometric characteristics of the stenosis, the presence of coronary collateral circulation, the microvascular component of coronary resistance, the presence of left ventricular hypertrophy modulating the myocardial extravascular component of coronary resistance, the viable or necrotic state of the myocardium distal to the stenosis, the presence of coronary macrovascular or microvascular spasm, and, last but not least, the presence of concomitant anti-ischemic therapy.Up to now, coronary flow reserve has been evaluated invasively i
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