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Search Results: 1 - 10 of 2993 matches for " Alicia Jorquera "
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Tratamiento. Enfermedad de Chagas
Alicia Jorquera
Biomédica , 2011,
La formación del investigador venezolano: Un tránsito a la luz del pensamiento complejo
Jorquera Fernández,Alicia;
Educere , 2009,
Abstract: with the theoretical-character group of elements reflectively tackled in this article, we face the challenge towards a new discourse fed by the intention of revolutionizing the possibilities for our usual pedagogical practices directed to the education of our new researchers. new educational thoughts, more related to social complexity, emerge by assuming the process of turning the man-individual into a man-social being, inviting us to be part of re-meaning scientists? academic competences within the referred contexts to appropriate and rescue the most unusual pedagogical relations that allow, sufficiently, open dialogs between ideas, considering the impact and importance of human affects and current insufficiencies to achieve a fairer comprehension of knowledge values within the area of our social reality.
Infectividad del perro (Canis familiaris) para Lutzomyia youngi en Trujillo, Venezuela
Hernández,Dalila; Rojas,Elina; Scorza,José Vicente; Jorquera,Alicia;
Biomédica , 2006,
Abstract: introduction. in trujillo, venezuela the prevalence for american tegumentary leishmaniasis (atl) is 38 per 100.000 inhabitants. objective. in a periurban, rural settlement of the capital city trujillo, we studied the potential capability of the domestic dog (canis familiaris) as a source of infection for lutzomyia youngi, a phlebotomine sand fly species abundant in the study area and whose domestic vectorial activity has been proven. materials and methods. dogs with dermal lesions suggestive of atl and parasitological confirmation of infection, were selected for xenodiagnosis by allowing sylvatic phlebotomines from a atl free area, to feed ad libitum over each animal′s entire body surface. the insects′ intestinal tracts were dissected 5 days after the blood meal in order to look for flagellate forms. when these were found, parasitological identification was performed by the multiplex-pcr technique. results. four hundred and fifty five sand flies engorged over two dogs in three different assays; promastigotes were found in 4 (0.88%) of the specimens on only one occasion. pcr identified dna of the leishmania viannia subgenus. conclusion. the household dog has the potential of being a domestic risk factor in the atl transmission cycle.
Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela
Jorquera, Alicia;González, Ricardo;Marchán-Marcano, Edgar;Oviedo, Milagros;Matos, Mercedes;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000100008
Abstract: we studied the natural infection of lutzomyia (lutzomyia) sp. with leishmania in endemic foci of cutaneous leishmaniasis in the paria peninsula, state of sucre, venezuela. sand flies were collected between march 2001 and june 2003, using shannon light-traps and human bait. of the 1291 insects captured, only two species of phlebotomines were identified: l. ovallesi (82.75%) and l. gomezi (17.42%). a sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-pcr assay for simultaneous detection of new word leishmaniaand viannia subgenera. the results showed a total of 8 pools (15.68%) infected; of these, 7 were l. ovallesi naturally infected with l. braziliensis (2 pools) and l. mexicana (5 pools) and 1 pool of l. gomezi infected by l. braziliensis.
Scorpion poisoning in the Acosta and Caripe Counties of Monagas State, Venezuela. part 1: characterization of some epidemiological aspects
De Sousa, Leonardo;Parrilla, Pedro;Tillero, Luis;Valdiviezo, Ana;Ledezma, Eliades;Jorquera, Alicia;Quiroga, Mercedes;
Cadernos de Saúde Pública , 1997, DOI: 10.1590/S0102-311X1997000100013
Abstract: scorpion poisoning was surveyed in acosta and caripe counties, located in the turimiquire subregion of monagas state, venezuela, aiming to expand information on stings by characterizing some epidemiological aspects. from 1987 to 1993, 298 cases of scorpion stings were recorded in acosta and caripe counties. acosta had 212 cases, with an annual incidence rate of 18.3?(cases per 10,000 inhabitants) and a period median incidence rate of 128?. the scorpion poisoning index is 4.18 times greater in acosta than in caripe, confirming the former as a hyperendemic zone for this type of injury, with a phenomenon of cyclical temporal fluctuations and an upward trend. this area is infested with scorpions of the genus tityus. our results show that the region surveyed in monagas state is endemic for scorpion stings, posing a major public health problem.
Seroprevalencia de la infección por Trypanosoma cruzi en la población rural de Miraflores, estado Monagas: Estabilidad y diferencia de reactividad de epimastigotes fijados
Berrizbeitia,Mariolga; Aguilera,Giovanna; Ward,Brian; Rodríguez,Jessicca; Jorquera,Alicia; Ndao,Momar;
Revista de la Sociedad Venezolana de Microbiología , 2010,
Abstract: the seroprevalence of anti-trypanosoma cruzi igg was evaluated in the rural population of miraflores, monagas state. the study included 106 individuals (ages: 4 to 79 years) of any sex. the serologic diagnosis was done through the elisa test using as antigen fixed t. cruzi epimastigote forms. the stability of the antigen used was evaluated, as well as the differences in reactivity of the two different t. cruzi strains used (the tulahuen-brasil complex, and the rg1 strains). seropositivity of anti-t. cruzi igg type antibodies with the rg1 strains and the mixture of tulahuen-brasil strains was 2.8%. there was a positive relationship between the age of patients and the optical density of the anti-t. cruzi igg type antibody determination shown by the elisa test for both strains. the tulahuen-brasil strains showed greater reactivity than the one seen with the rg1 strain. fixed antigens (mixture of tulahuen-brasil strains) maintained their stability and reactivity during a five-year period, which means that this antigen is an excellent candidate for diagnostic tests. the epidemiological risk variables for the reactivation of this infection were present in this region of venezuela.
Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease
Mariolga Berrizbeitia,Milagros Figueroa,Brian J. Ward,Jessicca Rodríguez,Alicia Jorquera,Maria A. Figuera,Leomerys Romero,Momar Ndao
Journal of Tropical Medicine , 2012, DOI: 10.1155/2012/875909
Abstract: An indirect enzyme-linked immunoabsorbent assay (ELISA) for Trypanosoma cruzi was developed using epimastigote secretion/excretion proteins (ESEA antigens) obtained from axenic culture supernatants. A panel of 120 serum samples from subjects with confirmed Chagas disease , healthy controls , and patients with other parasitic diseases was used to evaluate the new ESEA-based ELISA (ELISAESEA). This new test had excellent sensitivity (98%) and acceptable specificity (88%). Cross-reactivity was observed largely in sera from subjects with Leishmania and Ascaris infections. Using Western blotting and epimastigotes from two distinct T. cruzi isolates, several polypeptide bands with molecular masses ranging from 50 to 220?kDa were detected in pooled chagasic sera. However, the band pattern for each isolate was different. These data suggest that an inexpensive and technically simple ELISA based on ESEA antigens is a promising new tool for the diagnosis of Chagas disease. 1. Introduction Chagas disease, discovered by Dr. Carlos Chagas in 1909, is caused by the protozoan parasite, Trypanosoma cruzi. This parasite can be transmitted by triatomine vectors, blood transfusions, laboratory accidents, organ transplantation, or through vertical transmission. The disease is endemic in 18 Latin American countries. In 2008, the World Health Organization (WHO) estimates that ~8 million people were infected resulting in ~11?000 deaths per year [1]. Despite decades of effort, there is still no gold standard test for the diagnosis of Chagas disease and several international organizations have recently emphasized the need for improved serodiagnostic tests [1]. To this end, many companies and research groups have developed a range of parasitologic, serologic, and nucleic acid-based assays. The most common methods include the indirect immunofluorescence assay (IFA), indirect haemagglutination assay (IHA), and the enzyme-linked immunosorbent assay (ELISA) [2]. The target antigens in these latter assays have included whole fixed parasites [3], 67 and 90-kDa lectin purified proteins [4, 5], synthetic peptides [6], recombinant proteins [7–14], and trypomastigote excreted/secreted antigen (TESA). Of these antigens, TESA proteins have shown particular promise in different assay formats (ELISA-TESA, Western blot) and in several different laboratories and geographic settings [15–19]. Unfortunately, the production of TESA antigen requires adequate cell culture facilities and other sophisticated infrastructure that are not always available to laboratories in resource-poor settings. Thus, in
Hacia una política integral de juventud
Jimena Jorquera
Ultima Década , 2000,
On the entropy for group actions on the circle
Eduardo Jorquera
Mathematics , 2009,
Abstract: We show that for a finitely generated group of $C^2$ circle diffeomorphisms, the entropy of the action equals the entropy of the restriction of the action to the non-wandering set.
Trypanosoma cruzi Infection in an Indigenous Kari?a Community in Eastern Venezuela
Mariolga Berrizbeitia,Dairene Moreno,Brian J. Ward,Erika Gómez,Alicia Jorquera,Jessicca Rodríguez,Norys García,Melfran Herrera,Mercedes Marcano,Momar Ndao
Epidemiology Research International , 2012, DOI: 10.1155/2012/138259
Abstract: We investigated the seroprevalence of Trypanosoma cruzi infection in an indigenous Kari?a population in eastern Venezuela. A total of 175 serum samples were collected in the community of Pi?antal during February 2009. Interviews targeting socioeconomic and environmental factors associated with the T. cruzi transmission were also conducted. Samples were evaluated using trypomastigote excreted/secreted antigens (TESAs) in an ELISA format. TESA-ELISA positive samples were confirmed by indirect haemagglutination (HAI) (Wiener). A nonsystematic collection of vectors was also undertaken. T. cruzi seroprevalence was 7.43% according to both assays, and the mean age of infected patients was years (range 34 to 73 years). The vector infection rate was 20.00% (2/10). T. cruzi seropositivity was associated with a history of triatomine bites, the ability to recognize the vector and poor knowledge about Chagas disease, but no associations were found with gender, house type, knowledge of how the disease is transmitted, or the presence of vectors or animals inside dwellings. To our knowledge, this is the first study of the seroprevalence of T. cruzi in an indigenous population in eastern Venezuela. All of the epidemiological variables required for the establishment of active vectorial transmission of T. cruzi were present in this community. 1. Introduction The Southern Cone and Andean countries initiative proposed the reduction or interruption of the vectorial transmission of Chagas disease (CD) in several countries in Latin America such as Venezuela, Chile, Colombia, Uruguay, and regions of Brazil. This goal was achieved to the extent that some countries in South America have been declared free of the vectorial transmission of T. cruzi [1–3]. In Venezuela, the national Chagas Disease Control Program was started in 1966. This program reduced vectorial transmission through the use of insecticides, improvement of rural houses, education campaigns, and the screening of all public hospital blood banks for T. cruzi. In spite of these activities, several investigators raised the possibility that CD could reemerge in Venezuela [4, 5]. This fact has been demonstrated in several works: A?ez et al. [6] carried out a multicentric study where they evaluated 310 patients referred to a diagnostic center and reported 168 (54.19%) were seropositive for T. cruzi; of these, 75 (44.64%) were in the acute phase and 36.00% were children under 10 years old. Moreover, the serologic examination performed in 3,993 individuals from 75 rural areas in Venezuela showed 11.22% prevalence of T.
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